Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 83592055

GI number: 83592055

Start: 842843

End: 844156

Strand: Reverse

Name: purB [H]

Synonym: Rru_A0716

Alternate gene names: 83592055

Gene position: 844156-842843 (Counterclockwise)

Preceding gene: 83592056

Following gene: 83592054

Centisome position: 19.39

GC content: 65.53

Gene sequence:

>1314_bases
ATGATTCCCCGCTATAGCCGCGACCGCATGGCCGCTATTTGGGCCCCGGAAAACCGCTTCCGCATCTGGTTCCAGATCGA
AGCCCATGCCTGCGACGCCCTGGCGGCGATCGGCGTGATTCCGGCCGAGGCGGCGAAGACCGTCTGGGAAAAGGGCGACT
TGCCCTATACGCCCGAGCGCGTGGCGCGCATCGACGCCATCGAAGCCGAGGTCAAGCACGACGTCATCGCTTTCCTGACC
GAGCTTTCCGAACATGTCGGCGAGCCGGCGCGCTTCATCCATCAGGGCATGACCAGTTCGGACGTGCTCGATACCTGTCT
GGCCGTTCAGCTTACCCAGGCCGCCGATCTTCTGCTCGAGGATATCGACGCCCTGCTCGCCGCCCTGCGCACCCGCGCCT
TCGAGCATAAGATGACCGTCTGCATGGGCCGCAGCCACGGCATCCATGCCGAACCGGTGACCATGGGCCTGAAGTTCGCC
GGTTTCCACGCCGAGTTCCAGCGCGCCCGCACCCGCTTGGTCGCCGCCCGGGGCGAAATCGCCACCTGCGCCATCTCGGG
CGCCGTCGGCACCTTCGCCAATATCGATCCGCGGGTCGAAGAGCATGTCGCGGCCAAGCTGGGCTTGCTGCCCGAACCGC
TGTCGACCCAGGTGATCCCGCGTGATCGCCACGCCCAGTTCTTCGCCGTGCTCGGCGTCATCGCCAGTTCGGTCGAACGC
GCCGCCCTTGAAATCCGCCATCTCCAGCGCACCGAAGTGCGCGAGGTCGAGGAATTCTTCTCGAAGGGCCAGAAGGGCAG
CTCGGCCATGCCCCATAAGCGCAACCCGGTGCTGACCGAGAACCTGACCGGCCTTGCCCGCATGGTCCGCGCCTATGCCC
TGCCGGCGATGGAAAACGTCGCCCTCTGGCACGAGCGCGACATCTCGCATTCCTCGGTCGAACGGATGATCGGCCCCGAC
GCCACCGTCACCCTGGATTTCGCCCTCGCCCGGCTGACCGGGGTCTTCACCAATCTGGTGATCTATCCCGAGGCGATCGA
CCGCACCTTGAACCAGATGGGCGGGCTGGTCTTCAGCCAGCGCGTCCTGCTCGCCCTCACCCAGGCCGGGGTGTCGCGCG
AAGAGGCCTATCGCATCGTCCAGCGCAACGCCATGGAGGTGTGGAACGGCCAGGGCGGCCTGCTCGATCGCCTGAAAGCC
GATGCGGCGGTCACCGACAAGCTGACCCCGGAGGCTTTGGAAGAGCTGTTCGATCTGGGCTACCACACCAAACACGTCGA
CACCATCTTCCGTCGGGTCTTCGGCGACAATTAG

Upstream 100 bases:

>100_bases
GCCGCGCCCGAACGCCCTTATCGGCAGTTGGAGGGGCGGCCGGCTTGGCCTATAAGGAAGCCACCCATCCCCGCACCCGT
TCATGGAGCCTGAGACAGCG

Downstream 100 bases:

>100_bases
CCTAGGCAGCGGGTAACCTTGGAAGCGGGGCGCGCGGGTGGTCTATCGCAAGGACGACGATAAGGACGCGACGGCCCCGC
CGGCGGGGGCCGAGGCCCCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MIPRYSRDRMAAIWAPENRFRIWFQIEAHACDALAAIGVIPAEAAKTVWEKGDLPYTPERVARIDAIEAEVKHDVIAFLT
ELSEHVGEPARFIHQGMTSSDVLDTCLAVQLTQAADLLLEDIDALLAALRTRAFEHKMTVCMGRSHGIHAEPVTMGLKFA
GFHAEFQRARTRLVAARGEIATCAISGAVGTFANIDPRVEEHVAAKLGLLPEPLSTQVIPRDRHAQFFAVLGVIASSVER
AALEIRHLQRTEVREVEEFFSKGQKGSSAMPHKRNPVLTENLTGLARMVRAYALPAMENVALWHERDISHSSVERMIGPD
ATVTLDFALARLTGVFTNLVIYPEAIDRTLNQMGGLVFSQRVLLALTQAGVSREEAYRIVQRNAMEVWNGQGGLLDRLKA
DAAVTDKLTPEALEELFDLGYHTKHVDTIFRRVFGDN

Sequences:

>Translated_437_residues
MIPRYSRDRMAAIWAPENRFRIWFQIEAHACDALAAIGVIPAEAAKTVWEKGDLPYTPERVARIDAIEAEVKHDVIAFLT
ELSEHVGEPARFIHQGMTSSDVLDTCLAVQLTQAADLLLEDIDALLAALRTRAFEHKMTVCMGRSHGIHAEPVTMGLKFA
GFHAEFQRARTRLVAARGEIATCAISGAVGTFANIDPRVEEHVAAKLGLLPEPLSTQVIPRDRHAQFFAVLGVIASSVER
AALEIRHLQRTEVREVEEFFSKGQKGSSAMPHKRNPVLTENLTGLARMVRAYALPAMENVALWHERDISHSSVERMIGPD
ATVTLDFALARLTGVFTNLVIYPEAIDRTLNQMGGLVFSQRVLLALTQAGVSREEAYRIVQRNAMEVWNGQGGLLDRLKA
DAAVTDKLTPEALEELFDLGYHTKHVDTIFRRVFGDN
>Mature_437_residues
MIPRYSRDRMAAIWAPENRFRIWFQIEAHACDALAAIGVIPAEAAKTVWEKGDLPYTPERVARIDAIEAEVKHDVIAFLT
ELSEHVGEPARFIHQGMTSSDVLDTCLAVQLTQAADLLLEDIDALLAALRTRAFEHKMTVCMGRSHGIHAEPVTMGLKFA
GFHAEFQRARTRLVAARGEIATCAISGAVGTFANIDPRVEEHVAAKLGLLPEPLSTQVIPRDRHAQFFAVLGVIASSVER
AALEIRHLQRTEVREVEEFFSKGQKGSSAMPHKRNPVLTENLTGLARMVRAYALPAMENVALWHERDISHSSVERMIGPD
ATVTLDFALARLTGVFTNLVIYPEAIDRTLNQMGGLVFSQRVLLALTQAGVSREEAYRIVQRNAMEVWNGQGGLLDRLKA
DAAVTDKLTPEALEELFDLGYHTKHVDTIFRRVFGDN

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=417, Percent_Identity=27.0983213429257, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=27.154046997389, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1787376, Length=348, Percent_Identity=29.5977011494253, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17508577, Length=403, Percent_Identity=26.302729528536, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=349, Percent_Identity=27.2206303724928, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323391, Length=418, Percent_Identity=28.2296650717703, Blast_Score=143, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24647570, Length=404, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640177, Length=213, Percent_Identity=30.9859154929577, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640179, Length=213, Percent_Identity=30.9859154929577, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48470; Mature: 48470

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRDRMAAIWAPENRFRIWFQIEAHACDALAAIGVIPAEAAKTVWEKGDLPYTPER
CCCCCCCCCEEEEECCCCCEEEEEEEECHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHH
VARIDAIEAEVKHDVIAFLTELSEHVGEPARFIHQGMTSSDVLDTCLAVQLTQAADLLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DIDALLAALRTRAFEHKMTVCMGRSHGIHAEPVTMGLKFAGFHAEFQRARTRLVAARGEI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEECHHHHHHHHHHHHHHHCCCE
ATCAISGAVGTFANIDPRVEEHVAAKLGLLPEPLSTQVIPRDRHAQFFAVLGVIASSVER
EEEEECCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AALEIRHLQRTEVREVEEFFSKGQKGSSAMPHKRNPVLTENLTGLARMVRAYALPAMENV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHH
ALWHERDISHSSVERMIGPDATVTLDFALARLTGVFTNLVIYPEAIDRTLNQMGGLVFSQ
HHHHHCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RVLLALTQAGVSREEAYRIVQRNAMEVWNGQGGLLDRLKADAAVTDKLTPEALEELFDLG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCHHHHHHHHHCC
YHTKHVDTIFRRVFGDN
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYSRDRMAAIWAPENRFRIWFQIEAHACDALAAIGVIPAEAAKTVWEKGDLPYTPER
CCCCCCCCCEEEEECCCCCEEEEEEEECHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHH
VARIDAIEAEVKHDVIAFLTELSEHVGEPARFIHQGMTSSDVLDTCLAVQLTQAADLLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DIDALLAALRTRAFEHKMTVCMGRSHGIHAEPVTMGLKFAGFHAEFQRARTRLVAARGEI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEECHHHHHHHHHHHHHHHCCCE
ATCAISGAVGTFANIDPRVEEHVAAKLGLLPEPLSTQVIPRDRHAQFFAVLGVIASSVER
EEEEECCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AALEIRHLQRTEVREVEEFFSKGQKGSSAMPHKRNPVLTENLTGLARMVRAYALPAMENV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHH
ALWHERDISHSSVERMIGPDATVTLDFALARLTGVFTNLVIYPEAIDRTLNQMGGLVFSQ
HHHHHCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RVLLALTQAGVSREEAYRIVQRNAMEVWNGQGGLLDRLKADAAVTDKLTPEALEELFDLG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCHHHHHHHHHCC
YHTKHVDTIFRRVFGDN
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]