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The map label for this gene is zraS [H]

Identifier: 83591999

GI number: 83591999

Start: 783368

End: 785245

Strand: Reverse

Name: zraS [H]

Synonym: Rru_A0660

Alternate gene names: 83591999

Gene position: 785245-783368 (Counterclockwise)

Preceding gene: 83592000

Following gene: 83591998

Centisome position: 18.04

GC content: 63.68

Gene sequence:

>1878_bases
TTGGCCGGATCCGCACCACTCAATCGGTTTTTCCTGCTGACCCTGATCGGGCAGGTGGTCGCCCTTGCCGTCTATGCCGC
GATAACCTGGATGGATGTTCGCAACGACGCGATGATCCAGCTCCACTATACGGCGCTGACCTTCCGTTCGGCCTCGCAGA
CCACCTTCGCCGGCTTTGAAAGCTCTCTCGATGCCCTCGCCGATCAGTTGATCGACAGCGACGGGCTGAGCGACCCGACG
AAGGGCCGGGCGATCTTGACCAAAAGCCTTGGCACCCTGCCGGCTTTCACCGTCTTGTCCGTGATCGCCGTCGATGGTCA
GATCCGCGCCCTCAGCGAGGACAGCCACGATCCCCTTCCCAATCTTCTGACCAATCCCGACACTCGCGAAACCTTCCTGC
AAGCCCTGGATAGCAAGGCCGCGGTTTTCGGGGAGATTTATTACAGTCCGTTTTTGAAGCGCTGGGTTTTTCCCATCCGC
AAGGCCGTCCGCGACGAAAACGGAACCGTTATCGCCGTTGTCGCCGGGGGCATCGATTCCAATGCCGCCGTCAACGTCTG
GCGCGCCCTGGACGCCTTCGCCGATCAACGCATCTGGATGCTGCGCGCCGATCGCGGCGTGCAAAACATCCATCCGATCT
CCGCCGCCGACATGCGGCGCCTGGAGGGCACCGTTCTCCCCGCCCTCGCCGGCGGCTTCCCGCCCCAGGAGGGGCCGGCC
CCGCTGATCGACTGGCCGATCCTTGAAGACGAGATCGCCGTCACCCTGCCCTTCCCGGCGCTTTCGGGAACCATCGTCGC
TAGCATGAAGAAGTCGCTTCTCGTAAAAACCTGGGTCCACCGCATGGCGACGGGGGGGCTTGGCTTCGTCTTTCTCTGCG
TTGTCACCGCCGTGGTCTTCCGTCTTGCCCAAAGATCCCAGGCGGTGGCCGATGCCCGGCGCGAAGCGGCCGAGCACACC
TTGTTCGAGTCGCGCGAAAACTATCGTCTGCTCTATGAGAACGTCGAAACCCTGGTGAGGGACCGAACCGCCGAGTTGGC
GGCGCTGGTCGACACCTTGACCCGCACCCAAGCCGAATTGGTGCGCTCGGAAAAGCTGGCCTCGCTGGGGGCGATGGTCG
CCGCCGTGGCCCATGAGGTGAACACTCCCGTCGGCAACGCCCTGGTGGCGGCGACGACGCTGAAGGACCATACCCGCGCC
TTCGCCACCCTGACCGCCTCGGGGCGGATCTCCCGGCAAGCCATCGCCGCTTACGTCGCAACCACCGAGGAGGCCTGCGA
CCTGCTGGTTCGCAGCCTTGAACAGGCCGGCCGGCTTGTCGGTACCTTCAAGCAGGTGGCCGTCGATCAGACCAACGACC
AGCGCCGGAGCTTCCTTTTGGATCAGACGATCCAAGAGGTGATCACCGCGCTCAAGCCGTCGTTCCGCTATCGCCCGATC
ACCGTCAGCCTTGATCTCGCCGCCGCGGTCACGATGGACAGCTTTCCCGGATCCCTGGCCCAGGTCATCGGCAACCTTGT
CACCAATGCCGTTGTCCATGGCTTCGATGACCACGACTCCGGAGAGATCCGCATCATCACTCGCAGCGTAGAAGCCCAGT
CCGTGACCATTGGCGTCAGCGACAATGGCCGGGGCATCGCTCCGGCGATTCTGCCCAAAATCTTTGACCCCTTCTTTACG
ACCCGCCTGGGACAAGGGGGCAGCGGCCTGGGACTTCACATCGTCTATGGCATCGTCACCAAAATTCTTGGCGGTGGCCT
GACGGTGGACAGCGTCGAGGGCGAGGGAACCCGCTTTACCCTCACCCTGCCCCGCGTCGCCCCCGCCGAAAAGCCCGTCG
AGCAGCCCGTCCGGCCCAAGGAGGCCCTAACGCCATGA

Upstream 100 bases:

>100_bases
CCGATAGGTCGAAAGCCTATATTACCCAAAAGACAATTCCCCCAAAGTCTACAATCCCCTTATGCTCGCTCCCCGAAGGG
CGTCTTAGGGAGGCTTGGCC

Downstream 100 bases:

>100_bases
CACCGAATGAGGATCTGATAGAGATCTTCGAAGACGAGGAAATTTCCCCCTCCTTGACGGCCAAGACATGGCCGATCCTC
ATCGTCGATGATGACCAGGA

Product: periplasmic sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MAGSAPLNRFFLLTLIGQVVALAVYAAITWMDVRNDAMIQLHYTALTFRSASQTTFAGFESSLDALADQLIDSDGLSDPT
KGRAILTKSLGTLPAFTVLSVIAVDGQIRALSEDSHDPLPNLLTNPDTRETFLQALDSKAAVFGEIYYSPFLKRWVFPIR
KAVRDENGTVIAVVAGGIDSNAAVNVWRALDAFADQRIWMLRADRGVQNIHPISAADMRRLEGTVLPALAGGFPPQEGPA
PLIDWPILEDEIAVTLPFPALSGTIVASMKKSLLVKTWVHRMATGGLGFVFLCVVTAVVFRLAQRSQAVADARREAAEHT
LFESRENYRLLYENVETLVRDRTAELAALVDTLTRTQAELVRSEKLASLGAMVAAVAHEVNTPVGNALVAATTLKDHTRA
FATLTASGRISRQAIAAYVATTEEACDLLVRSLEQAGRLVGTFKQVAVDQTNDQRRSFLLDQTIQEVITALKPSFRYRPI
TVSLDLAAAVTMDSFPGSLAQVIGNLVTNAVVHGFDDHDSGEIRIITRSVEAQSVTIGVSDNGRGIAPAILPKIFDPFFT
TRLGQGGSGLGLHIVYGIVTKILGGGLTVDSVEGEGTRFTLTLPRVAPAEKPVEQPVRPKEALTP

Sequences:

>Translated_625_residues
MAGSAPLNRFFLLTLIGQVVALAVYAAITWMDVRNDAMIQLHYTALTFRSASQTTFAGFESSLDALADQLIDSDGLSDPT
KGRAILTKSLGTLPAFTVLSVIAVDGQIRALSEDSHDPLPNLLTNPDTRETFLQALDSKAAVFGEIYYSPFLKRWVFPIR
KAVRDENGTVIAVVAGGIDSNAAVNVWRALDAFADQRIWMLRADRGVQNIHPISAADMRRLEGTVLPALAGGFPPQEGPA
PLIDWPILEDEIAVTLPFPALSGTIVASMKKSLLVKTWVHRMATGGLGFVFLCVVTAVVFRLAQRSQAVADARREAAEHT
LFESRENYRLLYENVETLVRDRTAELAALVDTLTRTQAELVRSEKLASLGAMVAAVAHEVNTPVGNALVAATTLKDHTRA
FATLTASGRISRQAIAAYVATTEEACDLLVRSLEQAGRLVGTFKQVAVDQTNDQRRSFLLDQTIQEVITALKPSFRYRPI
TVSLDLAAAVTMDSFPGSLAQVIGNLVTNAVVHGFDDHDSGEIRIITRSVEAQSVTIGVSDNGRGIAPAILPKIFDPFFT
TRLGQGGSGLGLHIVYGIVTKILGGGLTVDSVEGEGTRFTLTLPRVAPAEKPVEQPVRPKEALTP
>Mature_624_residues
AGSAPLNRFFLLTLIGQVVALAVYAAITWMDVRNDAMIQLHYTALTFRSASQTTFAGFESSLDALADQLIDSDGLSDPTK
GRAILTKSLGTLPAFTVLSVIAVDGQIRALSEDSHDPLPNLLTNPDTRETFLQALDSKAAVFGEIYYSPFLKRWVFPIRK
AVRDENGTVIAVVAGGIDSNAAVNVWRALDAFADQRIWMLRADRGVQNIHPISAADMRRLEGTVLPALAGGFPPQEGPAP
LIDWPILEDEIAVTLPFPALSGTIVASMKKSLLVKTWVHRMATGGLGFVFLCVVTAVVFRLAQRSQAVADARREAAEHTL
FESRENYRLLYENVETLVRDRTAELAALVDTLTRTQAELVRSEKLASLGAMVAAVAHEVNTPVGNALVAATTLKDHTRAF
ATLTASGRISRQAIAAYVATTEEACDLLVRSLEQAGRLVGTFKQVAVDQTNDQRRSFLLDQTIQEVITALKPSFRYRPIT
VSLDLAAAVTMDSFPGSLAQVIGNLVTNAVVHGFDDHDSGEIRIITRSVEAQSVTIGVSDNGRGIAPAILPKIFDPFFTT
RLGQGGSGLGLHIVYGIVTKILGGGLTVDSVEGEGTRFTLTLPRVAPAEKPVEQPVRPKEALTP

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=251, Percent_Identity=29.4820717131474, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081816, Length=368, Percent_Identity=30.1630434782609, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788549, Length=290, Percent_Identity=26.8965517241379, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=312, Percent_Identity=25, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67344; Mature: 67213

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSAPLNRFFLLTLIGQVVALAVYAAITWMDVRNDAMIQLHYTALTFRSASQTTFAGFE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEEECCCCCCHHHHHH
SSLDALADQLIDSDGLSDPTKGRAILTKSLGTLPAFTVLSVIAVDGQIRALSEDSHDPLP
HHHHHHHHHHHCCCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHCCCCEEECCCCCCCCCC
NLLTNPDTRETFLQALDSKAAVFGEIYYSPFLKRWVFPIRKAVRDENGTVIAVVAGGIDS
HHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
NAAVNVWRALDAFADQRIWMLRADRGVQNIHPISAADMRRLEGTVLPALAGGFPPQEGPA
CHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHCCCCCCCCCC
PLIDWPILEDEIAVTLPFPALSGTIVASMKKSLLVKTWVHRMATGGLGFVFLCVVTAVVF
CCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RLAQRSQAVADARREAAEHTLFESRENYRLLYENVETLVRDRTAELAALVDTLTRTQAEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRSEKLASLGAMVAAVAHEVNTPVGNALVAATTLKDHTRAFATLTASGRISRQAIAAYVA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHEEEECCCCHHHHHHHHHHH
TTEEACDLLVRSLEQAGRLVGTFKQVAVDQTNDQRRSFLLDQTIQEVITALKPSFRYRPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TVSLDLAAAVTMDSFPGSLAQVIGNLVTNAVVHGFDDHDSGEIRIITRSVEAQSVTIGVS
EEEECHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEEC
DNGRGIAPAILPKIFDPFFTTRLGQGGSGLGLHIVYGIVTKILGGGLTVDSVEGEGTRFT
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCEEE
LTLPRVAPAEKPVEQPVRPKEALTP
EECCCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
AGSAPLNRFFLLTLIGQVVALAVYAAITWMDVRNDAMIQLHYTALTFRSASQTTFAGFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEEECCCCCCHHHHHH
SSLDALADQLIDSDGLSDPTKGRAILTKSLGTLPAFTVLSVIAVDGQIRALSEDSHDPLP
HHHHHHHHHHHCCCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHCCCCEEECCCCCCCCCC
NLLTNPDTRETFLQALDSKAAVFGEIYYSPFLKRWVFPIRKAVRDENGTVIAVVAGGIDS
HHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
NAAVNVWRALDAFADQRIWMLRADRGVQNIHPISAADMRRLEGTVLPALAGGFPPQEGPA
CHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHCCCCCCCCCC
PLIDWPILEDEIAVTLPFPALSGTIVASMKKSLLVKTWVHRMATGGLGFVFLCVVTAVVF
CCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RLAQRSQAVADARREAAEHTLFESRENYRLLYENVETLVRDRTAELAALVDTLTRTQAEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRSEKLASLGAMVAAVAHEVNTPVGNALVAATTLKDHTRAFATLTASGRISRQAIAAYVA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHEEEECCCCHHHHHHHHHHH
TTEEACDLLVRSLEQAGRLVGTFKQVAVDQTNDQRRSFLLDQTIQEVITALKPSFRYRPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TVSLDLAAAVTMDSFPGSLAQVIGNLVTNAVVHGFDDHDSGEIRIITRSVEAQSVTIGVS
EEEECHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEEC
DNGRGIAPAILPKIFDPFFTTRLGQGGSGLGLHIVYGIVTKILGGGLTVDSVEGEGTRFT
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCEEE
LTLPRVAPAEKPVEQPVRPKEALTP
EECCCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]