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The map label for this gene is iciA [H]

Identifier: 83591997

GI number: 83591997

Start: 780196

End: 781128

Strand: Direct

Name: iciA [H]

Synonym: Rru_A0658

Alternate gene names: 83591997

Gene position: 780196-781128 (Clockwise)

Preceding gene: 83591995

Following gene: 83592007

Centisome position: 17.92

GC content: 66.02

Gene sequence:

>933_bases
ATGATCGACTATCCCGCCGCCCTCGCCGTCGCCCTGGTCGTCCAGACGGGAAGCTTCGAGAAAGCGGCGAAAGCCCTGCA
TGTGACCCCCTCGGCCATCTCGCAGCGCGTCAAAATGATCGAGGACCGCTTGGGGGTCTCGCTGATCGAAAGGGGGACGC
CTTGTATCGCCACCGAGAAGGGCGAATGGTTGTGCCGGCATATGGACCATGTCGGCATGCTTGAAAAAGACCTGATCAAG
CACATGCCGGGTTTGGCCGAGGCGGGCGCCGACCCTTTGCACAGGGTGACGCTGACCCTGGCGACCAATGCCGACAGCCT
GGGAACATGGTTCCTTGGCGCGGTGGCGCCCTTCGCCCGGAGCACGGATTTTCTTGTGTCGATCGCCGTCGATGATGAGG
ATTACACGGCCGACTGGCTGCACCGCGGCCGGGTGCTCGCCGCCGTCACCTCTCTGGCCAAGCCGGTCAAGGGATGCCGG
GTGACGCGTCTGGGCGCCTTGCGCTATCAGGCGACGGCCAGCCCGGAGTTCTTTGAGCGCTATTTCGCCCGCGGCGTGAC
GCCCGAGACCCTCGCCCGGGCGCCGGCCCTGACCTTCAGCCAGAAGGACCGCCTTCAGCGCGACTGGATAAGGCGGACCT
TGGGCCAGTCCGTAAGCTTCCCCACCCACTGGCTGCCCTCGACGCAGGGCTTTGTCGATGCCTGTCTCGCCGGGATGGGA
TGGGGCATGAACCCCGCCCAGATGGTGGGCGCCCATCTGCAAGCGGGGCGTCTGGTCGAAATCCTGCCCGGGCGGACGCT
TGATAGTCCGCTGTTCTGGCAGGTGAACCGCCTTGCCGCCGATCAGCTTGCCGATCTGACGCGCGCGGTTGTCGGTCAGG
CGTCCCGGGCCCTGATACAGCCCTCCGCCGAGAGCGACCCGCCCGAGGTTTGA

Upstream 100 bases:

>100_bases
TCTGAGGACCACCGCTGTTTGACTTTACAGGGGGTGCGATAGCATGGTCCCATGAGTAAGCATAATTAAACAAACTAATC
AGACATTAGAGATTTTAACG

Downstream 100 bases:

>100_bases
CGGGCCCCGGCGTGGGTGCGGGCGAAGACCAGGAAATCCTCGGCGCTCAAGGGCTTGGAAAACAAATAGCCCTGGGCGGT
GAAACAGCCCATTTCGGTGA

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MIDYPAALAVALVVQTGSFEKAAKALHVTPSAISQRVKMIEDRLGVSLIERGTPCIATEKGEWLCRHMDHVGMLEKDLIK
HMPGLAEAGADPLHRVTLTLATNADSLGTWFLGAVAPFARSTDFLVSIAVDDEDYTADWLHRGRVLAAVTSLAKPVKGCR
VTRLGALRYQATASPEFFERYFARGVTPETLARAPALTFSQKDRLQRDWIRRTLGQSVSFPTHWLPSTQGFVDACLAGMG
WGMNPAQMVGAHLQAGRLVEILPGRTLDSPLFWQVNRLAADQLADLTRAVVGQASRALIQPSAESDPPEV

Sequences:

>Translated_310_residues
MIDYPAALAVALVVQTGSFEKAAKALHVTPSAISQRVKMIEDRLGVSLIERGTPCIATEKGEWLCRHMDHVGMLEKDLIK
HMPGLAEAGADPLHRVTLTLATNADSLGTWFLGAVAPFARSTDFLVSIAVDDEDYTADWLHRGRVLAAVTSLAKPVKGCR
VTRLGALRYQATASPEFFERYFARGVTPETLARAPALTFSQKDRLQRDWIRRTLGQSVSFPTHWLPSTQGFVDACLAGMG
WGMNPAQMVGAHLQAGRLVEILPGRTLDSPLFWQVNRLAADQLADLTRAVVGQASRALIQPSAESDPPEV
>Mature_310_residues
MIDYPAALAVALVVQTGSFEKAAKALHVTPSAISQRVKMIEDRLGVSLIERGTPCIATEKGEWLCRHMDHVGMLEKDLIK
HMPGLAEAGADPLHRVTLTLATNADSLGTWFLGAVAPFARSTDFLVSIAVDDEDYTADWLHRGRVLAAVTSLAKPVKGCR
VTRLGALRYQATASPEFFERYFARGVTPETLARAPALTFSQKDRLQRDWIRRTLGQSVSFPTHWLPSTQGFVDACLAGMG
WGMNPAQMVGAHLQAGRLVEILPGRTLDSPLFWQVNRLAADQLADLTRAVVGQASRALIQPSAESDPPEV

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=299, Percent_Identity=32.7759197324415, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33778; Mature: 33778

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYPAALAVALVVQTGSFEKAAKALHVTPSAISQRVKMIEDRLGVSLIERGTPCIATEK
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
GEWLCRHMDHVGMLEKDLIKHMPGLAEAGADPLHRVTLTLATNADSLGTWFLGAVAPFAR
HHHHHHHHHHHCHHHHHHHHHCCCHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHC
STDFLVSIAVDDEDYTADWLHRGRVLAAVTSLAKPVKGCRVTRLGALRYQATASPEFFER
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEECCCCHHHHHH
YFARGVTPETLARAPALTFSQKDRLQRDWIRRTLGQSVSFPTHWLPSTQGFVDACLAGMG
HHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
WGMNPAQMVGAHLQAGRLVEILPGRTLDSPLFWQVNRLAADQLADLTRAVVGQASRALIQ
CCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSAESDPPEV
CCCCCCCCCC
>Mature Secondary Structure
MIDYPAALAVALVVQTGSFEKAAKALHVTPSAISQRVKMIEDRLGVSLIERGTPCIATEK
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
GEWLCRHMDHVGMLEKDLIKHMPGLAEAGADPLHRVTLTLATNADSLGTWFLGAVAPFAR
HHHHHHHHHHHCHHHHHHHHHCCCHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHC
STDFLVSIAVDDEDYTADWLHRGRVLAAVTSLAKPVKGCRVTRLGALRYQATASPEFFER
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEECCCCHHHHHH
YFARGVTPETLARAPALTFSQKDRLQRDWIRRTLGQSVSFPTHWLPSTQGFVDACLAGMG
HHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
WGMNPAQMVGAHLQAGRLVEILPGRTLDSPLFWQVNRLAADQLADLTRAVVGQASRALIQ
CCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSAESDPPEV
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA