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The map label for this gene is cobS [H]

Identifier: 83592007

GI number: 83592007

Start: 791573

End: 792358

Strand: Direct

Name: cobS [H]

Synonym: Rru_A0668

Alternate gene names: 83592007

Gene position: 791573-792358 (Clockwise)

Preceding gene: 83591997

Following gene: 83592008

Centisome position: 18.19

GC content: 72.39

Gene sequence:

>786_bases
TTGACCGATCAAACCAAGCCTTCGTGGACTGCCGACCGTGGTGCCGATCTGCTCGGCGCCTTGTTCTTCCTGACCCGTTT
GCCGACGCCGACGACCCATACCCCGCCCCCCTTCACCCGGGCGGTCTGGGCCTTTCCGCTGGCCGGCGCCCTGGTTGGGT
TGATCGGCGCCCTGGCGGTGGGCGGTGCCCAAGCCCTGGGCCTGTCGCCGACCCTGGCCGCCCTGCTGGGCGTCAGCGTC
ATGGCCCTGGTCACCGGGGCCATGCACGAGGACGCCGTGGCCGATATCGCCGATGGCTTCGGCGGTGGACGGACGCGCGA
GCGGGTGCTTGAGATCATGCGCGACAGCCGGGTTGGCGCCTTTGGCGTCACCGCCCTGGTGCTGGTTTTGGCGCTGCGCG
TCGCCGCCCTCGCTGCCCTGGCCGGCGGACCCTGGGCGATGGCGGCGCTGGTCTGTGCCGCCGGCCTGGGGCGCGGCGCG
GCGGTGCTGATGCTGGGGTTGTTACCGCCGGCGCGCACCGATGGCCTGGGCGCCAGTTTCGGCCGGCCCCAACAGGCGTC
TTTGGCTTTCGCCCTGATCTTGTGCCTGGTGCTGGCGGTGGTTTTGTTGCCCGGCGCCGCCCCGGCCGTCGCCCTGGTCG
GCATGGTGGCGCCGACCCTTTATCTGGCTTGGCGGGCCAAGGTGCGGATCGGAGGGCAGACCGGCGATGTGGCCGGCGCC
GCCGCCCTGATCGGCGAGACCCTGGCCCTGGTCGGGCTGTCGGCGATGCTGGGGGCGGCCCCATGA

Upstream 100 bases:

>100_bases
TGATAAGGCGGGTGAGGACTGGCAAGACGCGGTACGATCCGCTATGGGAAGAGCCCCGGACGGGGCAAGCGGCCCGTTCT
TTGACTGGTGATTACACCCC

Downstream 100 bases:

>100_bases
GGACGCCGGGCGCGCGGCCGGCCGAGGCCACCCGCTGGTGGTGGATCCGCCATGCCCCGGTGCCCGATCCCGAGGGCCGC
ATTCTTGGCCAGCTTGATAC

Product: cobalamin-5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTDQTKPSWTADRGADLLGALFFLTRLPTPTTHTPPPFTRAVWAFPLAGALVGLIGALAVGGAQALGLSPTLAALLGVSV
MALVTGAMHEDAVADIADGFGGGRTRERVLEIMRDSRVGAFGVTALVLVLALRVAALAALAGGPWAMAALVCAAGLGRGA
AVLMLGLLPPARTDGLGASFGRPQQASLAFALILCLVLAVVLLPGAAPAVALVGMVAPTLYLAWRAKVRIGGQTGDVAGA
AALIGETLALVGLSAMLGAAP

Sequences:

>Translated_261_residues
MTDQTKPSWTADRGADLLGALFFLTRLPTPTTHTPPPFTRAVWAFPLAGALVGLIGALAVGGAQALGLSPTLAALLGVSV
MALVTGAMHEDAVADIADGFGGGRTRERVLEIMRDSRVGAFGVTALVLVLALRVAALAALAGGPWAMAALVCAAGLGRGA
AVLMLGLLPPARTDGLGASFGRPQQASLAFALILCLVLAVVLLPGAAPAVALVGMVAPTLYLAWRAKVRIGGQTGDVAGA
AALIGETLALVGLSAMLGAAP
>Mature_260_residues
TDQTKPSWTADRGADLLGALFFLTRLPTPTTHTPPPFTRAVWAFPLAGALVGLIGALAVGGAQALGLSPTLAALLGVSVM
ALVTGAMHEDAVADIADGFGGGRTRERVLEIMRDSRVGAFGVTALVLVLALRVAALAALAGGPWAMAALVCAAGLGRGAA
VLMLGLLPPARTDGLGASFGRPQQASLAFALILCLVLAVVLLPGAAPAVALVGMVAPTLYLAWRAKVRIGGQTGDVAGAA
ALIGETLALVGLSAMLGAAP

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=239, Percent_Identity=38.9121338912134, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 25967; Mature: 25836

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQTKPSWTADRGADLLGALFFLTRLPTPTTHTPPPFTRAVWAFPLAGALVGLIGALAV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GGAQALGLSPTLAALLGVSVMALVTGAMHEDAVADIADGFGGGRTRERVLEIMRDSRVGA
CCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCH
FGVTALVLVLALRVAALAALAGGPWAMAALVCAAGLGRGAAVLMLGLLPPARTDGLGASF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCC
GRPQQASLAFALILCLVLAVVLLPGAAPAVALVGMVAPTLYLAWRAKVRIGGQTGDVAGA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHH
AALIGETLALVGLSAMLGAAP
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDQTKPSWTADRGADLLGALFFLTRLPTPTTHTPPPFTRAVWAFPLAGALVGLIGALAV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GGAQALGLSPTLAALLGVSVMALVTGAMHEDAVADIADGFGGGRTRERVLEIMRDSRVGA
CCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCH
FGVTALVLVLALRVAALAALAGGPWAMAALVCAAGLGRGAAVLMLGLLPPARTDGLGASF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCC
GRPQQASLAFALILCLVLAVVLLPGAAPAVALVGMVAPTLYLAWRAKVRIGGQTGDVAGA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHH
AALIGETLALVGLSAMLGAAP
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]