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The map label for this gene is purD [H]

Identifier: 83591985

GI number: 83591985

Start: 767803

End: 769113

Strand: Direct

Name: purD [H]

Synonym: Rru_A0646

Alternate gene names: 83591985

Gene position: 767803-769113 (Clockwise)

Preceding gene: 83591984

Following gene: 83591986

Centisome position: 17.64

GC content: 71.32

Gene sequence:

>1311_bases
ATGCCCGGAGCCACGCCGATCAAGGTTCTTGTCGTCGGATCGGGGGGGCGCGAGCATGCGCTCTGCCGGAAACTCGTCGC
CTCGCCGCTCTGTGCGGATCTGCTGTGCGCGCCGGGGAACGCCGGAATCGCCGATGTGGCCCGCTGCCTGCCCGTCGGGG
TCGAGGAGATCGACGCCCTGGTCGCCCTGGCCAAGGCCGAGGCGGTCGGGCTGGTGGTCGTCGGTCCCGAGGCGCCGCTG
GTGCTGGGGCTGGCCGATAAGCTGGCCGAGGCGGGAATCGCCGTGTTCGGGCCAAGCCGGGCCGGCGCCCTGCTCGAAGG
CTCCAAGGGCTTCATGAAGGATGTGCTGGCCCGGGCCGGCGTCCCCACCGCCTGGTACGGGCGCTTCACCGATGCCGGGG
CGGCCAAGGCCTTCATTGGCGAAAAGGGCGCGCCGATCGTCGTCAAGACCGATGGTCTGGCGGCGGGCAAGGGCGTGATC
TTGTGCCAAACCCTTGAGGAGGGGGGCGCCGCCATCGATCTCCTGATGGGCGAGCGCGCCTTCGGCGAGGCCGGCGACGA
GGTGGTGATCGAGGAATTCCTTGTCGGCGAGGAGGCCAGCTTCTTCGCGCTGTGCGACGGCACCCGGGCGATCGCCTTTG
GCGCCGCCCAGGACCACAAGGCGGTGGGCGAGGGCGATACCGGCCCCAATACCGGCGGCATGGGCGCCTATTCGCCCGCT
CCGGTCTTCGATAAGGCGATGGAAGCCCAGGTCATGGCCGAGGTCATCGCCCCCACCTTGCGGGTGATGGCCGACATGGG
CCGGCCCTTCACCGGCGTGCTGTTCGCCGGCATCATGGTGACTCCGTCTGGCCCCAAGGTTCTGGAATTCAACGTGCGCT
TCGGCGATCCCGAATGTCAGGTGCTGCTGGCCCGCTTGGAAAGCGACCTGCTGCCGGTGCTGCTGGCCGCCGCCGAGGGG
CGTCTAGATAGGGTCGCGCCCTTGGTCTGGTCGCCGGAAACGGCGCTGACCGTGGTCATGGCCGCCAAGGGTTACCCGGG
CGAGTACGCCAAGGGCGACGTGATCGGCGGGCTGGCGGCGGCCGGGGAGGTTCGCGGCGCCGTCGTCTTCCATGCCGGGA
CCCGCGCCGATGGCGAGGGCCGGGTGGTGGCCGCCGGCGGCCGGGTGCTGGGCGTCACGGGCACGGGCGGCAGCGTGCGC
GAGGCCCGCGACCGCGCCTATCAGGCGGTCGACGCCCTGGACTGGCCGGGCGGCTTTTGCCGGCGTGATATCGGGTGGCG
GGCCCTGGCCCGCGAGGAGGGCGGACGATGA

Upstream 100 bases:

>100_bases
CCTGTTCAACTAAGCCGACCGGCGGCGCCCGCCCCGCTGGCGGGCGCTTCCTTCTTTTTTTGTTTTTTCGATCCTTCCCC
CAGACCTCGGAGACTCGCCG

Downstream 100 bases:

>100_bases
CCCGGCCCTTGCCCGAGACTCCCGGGGTCGGCGCCCAGGACGTGGTCGATGCCGGCCACCGCCTGAAGGGTCTTGTCGTC
ACCACGCCGCTTGTCGAAAG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPGATPIKVLVVGSGGREHALCRKLVASPLCADLLCAPGNAGIADVARCLPVGVEEIDALVALAKAEAVGLVVVGPEAPL
VLGLADKLAEAGIAVFGPSRAGALLEGSKGFMKDVLARAGVPTAWYGRFTDAGAAKAFIGEKGAPIVVKTDGLAAGKGVI
LCQTLEEGGAAIDLLMGERAFGEAGDEVVIEEFLVGEEASFFALCDGTRAIAFGAAQDHKAVGEGDTGPNTGGMGAYSPA
PVFDKAMEAQVMAEVIAPTLRVMADMGRPFTGVLFAGIMVTPSGPKVLEFNVRFGDPECQVLLARLESDLLPVLLAAAEG
RLDRVAPLVWSPETALTVVMAAKGYPGEYAKGDVIGGLAAAGEVRGAVVFHAGTRADGEGRVVAAGGRVLGVTGTGGSVR
EARDRAYQAVDALDWPGGFCRRDIGWRALAREEGGR

Sequences:

>Translated_436_residues
MPGATPIKVLVVGSGGREHALCRKLVASPLCADLLCAPGNAGIADVARCLPVGVEEIDALVALAKAEAVGLVVVGPEAPL
VLGLADKLAEAGIAVFGPSRAGALLEGSKGFMKDVLARAGVPTAWYGRFTDAGAAKAFIGEKGAPIVVKTDGLAAGKGVI
LCQTLEEGGAAIDLLMGERAFGEAGDEVVIEEFLVGEEASFFALCDGTRAIAFGAAQDHKAVGEGDTGPNTGGMGAYSPA
PVFDKAMEAQVMAEVIAPTLRVMADMGRPFTGVLFAGIMVTPSGPKVLEFNVRFGDPECQVLLARLESDLLPVLLAAAEG
RLDRVAPLVWSPETALTVVMAAKGYPGEYAKGDVIGGLAAAGEVRGAVVFHAGTRADGEGRVVAAGGRVLGVTGTGGSVR
EARDRAYQAVDALDWPGGFCRRDIGWRALAREEGGR
>Mature_435_residues
PGATPIKVLVVGSGGREHALCRKLVASPLCADLLCAPGNAGIADVARCLPVGVEEIDALVALAKAEAVGLVVVGPEAPLV
LGLADKLAEAGIAVFGPSRAGALLEGSKGFMKDVLARAGVPTAWYGRFTDAGAAKAFIGEKGAPIVVKTDGLAAGKGVIL
CQTLEEGGAAIDLLMGERAFGEAGDEVVIEEFLVGEEASFFALCDGTRAIAFGAAQDHKAVGEGDTGPNTGGMGAYSPAP
VFDKAMEAQVMAEVIAPTLRVMADMGRPFTGVLFAGIMVTPSGPKVLEFNVRFGDPECQVLLARLESDLLPVLLAAAEGR
LDRVAPLVWSPETALTVVMAAKGYPGEYAKGDVIGGLAAAGEVRGAVVFHAGTRADGEGRVVAAGGRVLGVTGTGGSVRE
ARDRAYQAVDALDWPGGFCRRDIGWRALAREEGGR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=46.4788732394366, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=46.4788732394366, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=46.4788732394366, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=46.4788732394366, Blast_Score=345, Evalue=4e-95,
Organism=Escherichia coli, GI1790438, Length=431, Percent_Identity=51.9721577726218, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=38.1176470588235, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=41.1633109619687, Blast_Score=314, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=43.6194895591647, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=43.6194895591647, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44473; Mature: 44341

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGATPIKVLVVGSGGREHALCRKLVASPLCADLLCAPGNAGIADVARCLPVGVEEIDAL
CCCCCEEEEEEECCCCCHHHHHHHHHHCHHHHHHEECCCCCCHHHHHHHHCCCHHHHHHH
VALAKAEAVGLVVVGPEAPLVLGLADKLAEAGIAVFGPSRAGALLEGSKGFMKDVLARAG
HHHHHHCEEEEEEECCCCCEEEEHHHHHHHCCEEEECCCCCCCEEECCCHHHHHHHHHCC
VPTAWYGRFTDAGAAKAFIGEKGAPIVVKTDGLAAGKGVILCQTLEEGGAAIDLLMGERA
CCCHHCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCHH
FGEAGDEVVIEEFLVGEEASFFALCDGTRAIAFGAAQDHKAVGEGDTGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PVFDKAMEAQVMAEVIAPTLRVMADMGRPFTGVLFAGIMVTPSGPKVLEFNVRFGDPECQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCEEEEEEEEECCHHHH
VLLARLESDLLPVLLAAAEGRLDRVAPLVWSPETALTVVMAAKGYPGEYAKGDVIGGLAA
HHHHHHHHHHHHHHHHHHCCCHHHCCCEEECCCHHEEEEEEECCCCCCCCCCCHHCHHHH
AGEVRGAVVFHAGTRADGEGRVVAAGGRVLGVTGTGGSVREARDRAYQAVDALDWPGGFC
CCCCCEEEEEEECCCCCCCCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHH
RRDIGWRALAREEGGR
HHCCCHHHHHHCCCCC
>Mature Secondary Structure 
PGATPIKVLVVGSGGREHALCRKLVASPLCADLLCAPGNAGIADVARCLPVGVEEIDAL
CCCCEEEEEEECCCCCHHHHHHHHHHCHHHHHHEECCCCCCHHHHHHHHCCCHHHHHHH
VALAKAEAVGLVVVGPEAPLVLGLADKLAEAGIAVFGPSRAGALLEGSKGFMKDVLARAG
HHHHHHCEEEEEEECCCCCEEEEHHHHHHHCCEEEECCCCCCCEEECCCHHHHHHHHHCC
VPTAWYGRFTDAGAAKAFIGEKGAPIVVKTDGLAAGKGVILCQTLEEGGAAIDLLMGERA
CCCHHCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCHH
FGEAGDEVVIEEFLVGEEASFFALCDGTRAIAFGAAQDHKAVGEGDTGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PVFDKAMEAQVMAEVIAPTLRVMADMGRPFTGVLFAGIMVTPSGPKVLEFNVRFGDPECQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCEEEEEEEEECCHHHH
VLLARLESDLLPVLLAAAEGRLDRVAPLVWSPETALTVVMAAKGYPGEYAKGDVIGGLAA
HHHHHHHHHHHHHHHHHHCCCHHHCCCEEECCCHHEEEEEEECCCCCCCCCCCHHCHHHH
AGEVRGAVVFHAGTRADGEGRVVAAGGRVLGVTGTGGSVREARDRAYQAVDALDWPGGFC
CCCCCEEEEEEECCCCCCCCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHH
RRDIGWRALAREEGGR
HHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]