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The map label for this gene is moaA [H]

Identifier: 83591665

GI number: 83591665

Start: 394969

End: 395976

Strand: Direct

Name: moaA [H]

Synonym: Rru_A0325

Alternate gene names: 83591665

Gene position: 394969-395976 (Clockwise)

Preceding gene: 83591664

Following gene: 83591666

Centisome position: 9.07

GC content: 68.15

Gene sequence:

>1008_bases
ATGGGGGCGACCGGTCGTCTGGTTGACCGCTTCGGTCGCGCGATCACCTATCTGCGTCTCTCGGTGACCGACCGCTGCAA
TTTTCGCTGCGTCTATTGCATGGGCGAGGACATCACCTTCCTGCCCATGAGAGATCTGCTGACCATCGAGGAACTCGACC
GGCTGTGCGCCGCCTTCATCGGGCTCGGGGTGCGCAAGCTGCGGCTGACCGGAGGGGAGCCGTTGGTGCGGCGCGGGGTC
GAAACCCTGATCGGTCACCTGGGCGAGCATGTGGCCGCCGGGCGGCTTGACGAACTGACCCTGACCACCAACGGCGCCCG
CTTGGCGGCCTTCGCGCCGGGGCTGGTCCTGGCCGGAGTGCGCCGGGTCAATGTCTCGCTCGATACCCTGGACGGTGACC
GCTTTCGCCAACTGACCCGCCTGGGCGATCTCGAGACGGTTCTGGCCGGCATCGCCGCCGCCAAGGCCCAGGGTCTGGCG
GTCAAGATCAACGCCGTGGTGTTCGAGGGCGGTCCCGAAGGCGATATCGACGCCCTGATCGCCTGGTGCGGCGCCCAGGG
TCACGATCTGACCCTGATCGAAGCCATGCCCTTTGGCGGCATGGCCGGGCTGGGCGACGGCCATCGGTCGTTCCTGGAGG
GCTTGCGCCGACGTTTGGCCGAACGCTGGTCTTTCGAGGCCTGCGATCACGCCACCGGTGGACCGGCGCGCTATGTGCGG
GTCGCCCAGACCGGCGGTCGCCTTGGCTTCATCGCGCCGATCAGCGGCTGCTTCTGCGACGGCTGCAACCGGGTGCGCGT
TACCTGCACCGGCGCGCTCCACCCCTGCCTTGATGGCGGAGCGACCGCCGATCTGCGGGCGGCCTTGCGCGCGGGCGAGT
CCAACGGTCCGCTCGAGCGGGCGATCGACGAAGGACTGCGCGCCAAGCCCCGGGGCCATGGCTTTAACACCCTGATCGAG
CATGGAAGAGTGAGCCTGGGGCGCCATATGAGTGTTACGGGAGGCTGA

Upstream 100 bases:

>100_bases
CTTAGGCGTTAACCGGGGAGCCCGGGTCCTGGCCGCCTTCCAGGATCCGGGCCCTTCCGGCGGGCGCCGAGGGTCGTTTC
TTTCCTTAAGGAGATGAGCC

Downstream 100 bases:

>100_bases
GTGTGACTCTGTTTGAAAGGGGTGCTAATACCCGCTAAGTTCCGCGTCGACTCGCGCCGGCGAACACGAGAAAATACAAA
TCGACCCCGAAACGAAATGC

Product: molybdenum cofactor biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MGATGRLVDRFGRAITYLRLSVTDRCNFRCVYCMGEDITFLPMRDLLTIEELDRLCAAFIGLGVRKLRLTGGEPLVRRGV
ETLIGHLGEHVAAGRLDELTLTTNGARLAAFAPGLVLAGVRRVNVSLDTLDGDRFRQLTRLGDLETVLAGIAAAKAQGLA
VKINAVVFEGGPEGDIDALIAWCGAQGHDLTLIEAMPFGGMAGLGDGHRSFLEGLRRRLAERWSFEACDHATGGPARYVR
VAQTGGRLGFIAPISGCFCDGCNRVRVTCTGALHPCLDGGATADLRAALRAGESNGPLERAIDEGLRAKPRGHGFNTLIE
HGRVSLGRHMSVTGG

Sequences:

>Translated_335_residues
MGATGRLVDRFGRAITYLRLSVTDRCNFRCVYCMGEDITFLPMRDLLTIEELDRLCAAFIGLGVRKLRLTGGEPLVRRGV
ETLIGHLGEHVAAGRLDELTLTTNGARLAAFAPGLVLAGVRRVNVSLDTLDGDRFRQLTRLGDLETVLAGIAAAKAQGLA
VKINAVVFEGGPEGDIDALIAWCGAQGHDLTLIEAMPFGGMAGLGDGHRSFLEGLRRRLAERWSFEACDHATGGPARYVR
VAQTGGRLGFIAPISGCFCDGCNRVRVTCTGALHPCLDGGATADLRAALRAGESNGPLERAIDEGLRAKPRGHGFNTLIE
HGRVSLGRHMSVTGG
>Mature_334_residues
GATGRLVDRFGRAITYLRLSVTDRCNFRCVYCMGEDITFLPMRDLLTIEELDRLCAAFIGLGVRKLRLTGGEPLVRRGVE
TLIGHLGEHVAAGRLDELTLTTNGARLAAFAPGLVLAGVRRVNVSLDTLDGDRFRQLTRLGDLETVLAGIAAAKAQGLAV
KINAVVFEGGPEGDIDALIAWCGAQGHDLTLIEAMPFGGMAGLGDGHRSFLEGLRRRLAERWSFEACDHATGGPARYVRV
AQTGGRLGFIAPISGCFCDGCNRVRVTCTGALHPCLDGGATADLRAALRAGESNGPLERAIDEGLRAKPRGHGFNTLIEH
GRVSLGRHMSVTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=313, Percent_Identity=36.4217252396166, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI22027536, Length=310, Percent_Identity=36.1290322580645, Blast_Score=174, Evalue=7e-44,
Organism=Escherichia coli, GI1786999, Length=334, Percent_Identity=33.8323353293413, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI32566331, Length=316, Percent_Identity=33.8607594936709, Blast_Score=180, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17561210, Length=318, Percent_Identity=33.9622641509434, Blast_Score=180, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI32566329, Length=316, Percent_Identity=33.5443037974684, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28574490, Length=286, Percent_Identity=38.1118881118881, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28574488, Length=286, Percent_Identity=38.1118881118881, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 35656; Mature: 35524

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGATGRLVDRFGRAITYLRLSVTDRCNFRCVYCMGEDITFLPMRDLLTIEELDRLCAAFI
CCCCHHHHHHHHHHEEEEEEEECCCCCEEEEEEECCCEEEECHHHHHHHHHHHHHHHHHH
GLGVRKLRLTGGEPLVRRGVETLIGHLGEHVAAGRLDELTLTTNGARLAAFAPGLVLAGV
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEHHCCHHHHHCC
RRVNVSLDTLDGDRFRQLTRLGDLETVLAGIAAAKAQGLAVKINAVVFEGGPEGDIDALI
EEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCHHHHH
AWCGAQGHDLTLIEAMPFGGMAGLGDGHRSFLEGLRRRLAERWSFEACDHATGGPARYVR
HHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEE
VAQTGGRLGFIAPISGCFCDGCNRVRVTCTGALHPCLDGGATADLRAALRAGESNGPLER
EEECCCCEEEECCCCCHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCHHH
AIDEGLRAKPRGHGFNTLIEHGRVSLGRHMSVTGG
HHHCCCCCCCCCCCHHHHHHCCCCCCCCEEECCCC
>Mature Secondary Structure 
GATGRLVDRFGRAITYLRLSVTDRCNFRCVYCMGEDITFLPMRDLLTIEELDRLCAAFI
CCCHHHHHHHHHHEEEEEEEECCCCCEEEEEEECCCEEEECHHHHHHHHHHHHHHHHHH
GLGVRKLRLTGGEPLVRRGVETLIGHLGEHVAAGRLDELTLTTNGARLAAFAPGLVLAGV
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEHHCCHHHHHCC
RRVNVSLDTLDGDRFRQLTRLGDLETVLAGIAAAKAQGLAVKINAVVFEGGPEGDIDALI
EEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCHHHHH
AWCGAQGHDLTLIEAMPFGGMAGLGDGHRSFLEGLRRRLAERWSFEACDHATGGPARYVR
HHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEE
VAQTGGRLGFIAPISGCFCDGCNRVRVTCTGALHPCLDGGATADLRAALRAGESNGPLER
EEECCCCEEEECCCCCHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCHHH
AIDEGLRAKPRGHGFNTLIEHGRVSLGRHMSVTGG
HHHCCCCCCCCCCCHHHHHHCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA