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The map label for this gene is fhlA [H]

Identifier: 83591666

GI number: 83591666

Start: 396246

End: 398447

Strand: Direct

Name: fhlA [H]

Synonym: Rru_A0326

Alternate gene names: 83591666

Gene position: 396246-398447 (Clockwise)

Preceding gene: 83591665

Following gene: 83591667

Centisome position: 9.1

GC content: 63.9

Gene sequence:

>2202_bases
ATGGGTGAGGCGCACAGTCTGGCGGAACGCTACGAACTGTTGCTCGATGTGTCGGAGATTCTCGAGCTGAACCGCGATCT
AAATTCGCTGCTCGGCGCGCTTTGCGACCTGCCGGCCAGCATTGGTCTGTTCGATTATGTCAATCTGCTGCTCTATGACG
CGCCCACCGATACGATGACGTTGTTTTCGCAGGGCGATGTCGGCGGCGGCTATGCGCGGATCGACGGTCTCGCCCCGACC
GAGGGACCGGGGCAGGTGGTCTGGGCCCGTCAACGTCCCTTCGTGTGCAGCGGCGAGGAATACCGCCGGCATTTCCTGAA
GATCGCCGCTCTGCGCCGCAACCAGCGGGTCGGACTGTATTGTCAGATGCCGCTGACCACCAGCCAGCGCCGGCTGGGCG
GGGTGGAATTCATCAGTTCACGCCCCACTACCTATGACGAGGATGATCTGACCTTCCTGCGCCGGGTCGCCTCGGTCATC
GCCATTTCGGTTGAAAACGCCCTGATCGGCGAAATGCGCCAGATGGAGGAGGAGCGTCTGCGCGGCGAACGCGATCACAA
CCACATTCTGGTCGAGGTGACCAATGCGGTGATTTCCAAGCTGAAGCTGGAGGATCTGGTCGACGAGGTGTCGCGCTCCA
TTCACCGGTTTTTCGGCGTCGATTTCGTCTCCCTCGATCTGGTCGATCCGGCCACCGCCACCATCAAATCGCGCTCGGTC
TATTTCCAGAAGGGGTTGGGGCCGGTCTGGGGCTCGGCCCAGGGGCCGCTTGGCGCCTCGCCGATGGCACCCTTGTTCGA
GACCAAGGCGCCGCGCATCCTGGCCCGCGCCGATCTGGGGGCCCTGGCGCGCGGCCATGATCAGATGACCTTGCTGACCA
ACGCCGGTTTCCAAGTGGCTTGCGGTATCCCGTTGCTTTCGGCGGGGCATGTTCTGGGGGTGCTGACCCTGGCCAGCCGC
GAGCGCGACAAGTTCACCGATTACAATATCGGCTTGCTGGGCGAGGTCGCCTCGCGCATTTGCATCGCCGTGGAAAACGC
CCTGGCTTACGGCGAGATTTCCCGGCTCAAGGACCAGTTGTCGCGCGAGAACAAGCAACTGGCCAAGGAACGCTATTACC
TGGAAGAGGAAATCCGCCGCACCGAGGATTTCGGCGATATCATCGGCATCAGCCCGGCCATTCGCGGCGTTCTCGAACAG
GTGGAGCTGGTCGCCGACAGCGACAGCACGGTGCTGATCCTCGGCGAGACGGGCACGGGCAAGGAACTGATCGCGCGCGC
CATCCATATGCTGAGCCAGCGCAAGAAGCGCACGATGATCAAGGTGAACTGCGCGGCCATTCCCTCGGCCCTGCTGGAAA
GCGATCTGTTCGGCCATGAAAAAGGCGCCTTCACCGGCGCCATCGCCCAGAAGTCCGGCCGCTTCGAACTGGCCGATCGC
GGCACGCTGTTTCTTGACGAGGTCGGCGACATCCCCATCGATCTGCAGCCCAAACTGCTGCGCGCCTTGCAGGAGCGCGA
AATCGAGCGGGTCGGCGGACATCAGCCGATCCCAGTGGATGTGCGGATGATCGCCGCCACCAACCGCGACCTGACGGGCA
TGGTCGAGAAGCGGCTGTTCCGGGGCGATCTGTATTATCGCCTGAACGTCTTTCCGATTCTGATCCCGCCTTTGCGCGAG
CGCCGCGAGGATATTCCGCTGATGGCGGATTATTTCATGCGCAAGACGGCGCGCAAGATGAACCGGGCGATCGAGGCCTT
CTCGGCCGAAACCATGGATATGCTGCTTAACTTGCCGTGGCCGGGCAATGTGCGCGAGCTGCAAAACGTCATCGAGCGCG
CGGTCATCCTGACCAAGGGGCCGGTGCTGCATCTGCCCGCCACCGAACTGCGCCATCATCTGGCGCCCAGCGCCGCCCTG
CCGTCCGAAGACGCGGTGATTCCTCTCCGCGCCGCGGTGCTGCCCTCCGCCGCGAGCGCGCCCGATCCGCTCCCGCCCCC
GGCCGGGTCGTCCCCCAATCCCGGCGATCTCGCCGGCGAGGCGATCGAGCGCGACCAGATCTTGCGAGTATTGCGCGAAA
CCAATGGCATCGTCGGTGGTCCGCGCGGCGCGGCGGCCCATCTCGGAATGAAGCGGACCACCTTGCTGTCGCGGATGCGC
CGCCTTGGCATCTCGCCCAAGGAGGTCGATCACCGCGCGTGA

Upstream 100 bases:

>100_bases
TTTGACTGGGAGTGGGGCAACCATACGGGGTGTAATGGGGAAAATAACCCTAAAATATCTCTGGTTAGCCGAATAGCCAT
CGGCGAAGGATCGTTTGGAC

Downstream 100 bases:

>100_bases
GGGGGGGCGCTAGCGGCCGCCAACAATCGTGGCAACACCTTCCCCTGCTGTGATACCGTTTCGCACAGGCGGAAAAGGGG
AAGTGCTCGGCGTCGTCGGA

Product: NifA subfamily transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MGEAHSLAERYELLLDVSEILELNRDLNSLLGALCDLPASIGLFDYVNLLLYDAPTDTMTLFSQGDVGGGYARIDGLAPT
EGPGQVVWARQRPFVCSGEEYRRHFLKIAALRRNQRVGLYCQMPLTTSQRRLGGVEFISSRPTTYDEDDLTFLRRVASVI
AISVENALIGEMRQMEEERLRGERDHNHILVEVTNAVISKLKLEDLVDEVSRSIHRFFGVDFVSLDLVDPATATIKSRSV
YFQKGLGPVWGSAQGPLGASPMAPLFETKAPRILARADLGALARGHDQMTLLTNAGFQVACGIPLLSAGHVLGVLTLASR
ERDKFTDYNIGLLGEVASRICIAVENALAYGEISRLKDQLSRENKQLAKERYYLEEEIRRTEDFGDIIGISPAIRGVLEQ
VELVADSDSTVLILGETGTGKELIARAIHMLSQRKKRTMIKVNCAAIPSALLESDLFGHEKGAFTGAIAQKSGRFELADR
GTLFLDEVGDIPIDLQPKLLRALQEREIERVGGHQPIPVDVRMIAATNRDLTGMVEKRLFRGDLYYRLNVFPILIPPLRE
RREDIPLMADYFMRKTARKMNRAIEAFSAETMDMLLNLPWPGNVRELQNVIERAVILTKGPVLHLPATELRHHLAPSAAL
PSEDAVIPLRAAVLPSAASAPDPLPPPAGSSPNPGDLAGEAIERDQILRVLRETNGIVGGPRGAAAHLGMKRTTLLSRMR
RLGISPKEVDHRA

Sequences:

>Translated_733_residues
MGEAHSLAERYELLLDVSEILELNRDLNSLLGALCDLPASIGLFDYVNLLLYDAPTDTMTLFSQGDVGGGYARIDGLAPT
EGPGQVVWARQRPFVCSGEEYRRHFLKIAALRRNQRVGLYCQMPLTTSQRRLGGVEFISSRPTTYDEDDLTFLRRVASVI
AISVENALIGEMRQMEEERLRGERDHNHILVEVTNAVISKLKLEDLVDEVSRSIHRFFGVDFVSLDLVDPATATIKSRSV
YFQKGLGPVWGSAQGPLGASPMAPLFETKAPRILARADLGALARGHDQMTLLTNAGFQVACGIPLLSAGHVLGVLTLASR
ERDKFTDYNIGLLGEVASRICIAVENALAYGEISRLKDQLSRENKQLAKERYYLEEEIRRTEDFGDIIGISPAIRGVLEQ
VELVADSDSTVLILGETGTGKELIARAIHMLSQRKKRTMIKVNCAAIPSALLESDLFGHEKGAFTGAIAQKSGRFELADR
GTLFLDEVGDIPIDLQPKLLRALQEREIERVGGHQPIPVDVRMIAATNRDLTGMVEKRLFRGDLYYRLNVFPILIPPLRE
RREDIPLMADYFMRKTARKMNRAIEAFSAETMDMLLNLPWPGNVRELQNVIERAVILTKGPVLHLPATELRHHLAPSAAL
PSEDAVIPLRAAVLPSAASAPDPLPPPAGSSPNPGDLAGEAIERDQILRVLRETNGIVGGPRGAAAHLGMKRTTLLSRMR
RLGISPKEVDHRA
>Mature_732_residues
GEAHSLAERYELLLDVSEILELNRDLNSLLGALCDLPASIGLFDYVNLLLYDAPTDTMTLFSQGDVGGGYARIDGLAPTE
GPGQVVWARQRPFVCSGEEYRRHFLKIAALRRNQRVGLYCQMPLTTSQRRLGGVEFISSRPTTYDEDDLTFLRRVASVIA
ISVENALIGEMRQMEEERLRGERDHNHILVEVTNAVISKLKLEDLVDEVSRSIHRFFGVDFVSLDLVDPATATIKSRSVY
FQKGLGPVWGSAQGPLGASPMAPLFETKAPRILARADLGALARGHDQMTLLTNAGFQVACGIPLLSAGHVLGVLTLASRE
RDKFTDYNIGLLGEVASRICIAVENALAYGEISRLKDQLSRENKQLAKERYYLEEEIRRTEDFGDIIGISPAIRGVLEQV
ELVADSDSTVLILGETGTGKELIARAIHMLSQRKKRTMIKVNCAAIPSALLESDLFGHEKGAFTGAIAQKSGRFELADRG
TLFLDEVGDIPIDLQPKLLRALQEREIERVGGHQPIPVDVRMIAATNRDLTGMVEKRLFRGDLYYRLNVFPILIPPLRER
REDIPLMADYFMRKTARKMNRAIEAFSAETMDMLLNLPWPGNVRELQNVIERAVILTKGPVLHLPATELRHHLAPSAALP
SEDAVIPLRAAVLPSAASAPDPLPPPAGSSPNPGDLAGEAIERDQILRVLRETNGIVGGPRGAAAHLGMKRTTLLSRMRR
LGISPKEVDHRA

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=716, Percent_Identity=44.5530726256983, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI87082117, Length=612, Percent_Identity=47.2222222222222, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1788550, Length=365, Percent_Identity=44.1095890410959, Blast_Score=290, Evalue=3e-79,
Organism=Escherichia coli, GI1790437, Length=338, Percent_Identity=47.3372781065089, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1788905, Length=237, Percent_Identity=51.4767932489451, Blast_Score=249, Evalue=5e-67,
Organism=Escherichia coli, GI87082152, Length=367, Percent_Identity=40.8719346049046, Blast_Score=245, Evalue=6e-66,
Organism=Escherichia coli, GI1790299, Length=342, Percent_Identity=42.1052631578947, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1789233, Length=239, Percent_Identity=47.6987447698745, Blast_Score=230, Evalue=3e-61,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=50.6493506493506, Blast_Score=222, Evalue=7e-59,
Organism=Escherichia coli, GI1786524, Length=350, Percent_Identity=36.8571428571429, Blast_Score=213, Evalue=5e-56,
Organism=Escherichia coli, GI1787583, Length=345, Percent_Identity=34.4927536231884, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI1789828, Length=285, Percent_Identity=35.0877192982456, Blast_Score=144, Evalue=3e-35,
Organism=Escherichia coli, GI87081858, Length=341, Percent_Identity=29.6187683284457, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 80824; Mature: 80693

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEAHSLAERYELLLDVSEILELNRDLNSLLGALCDLPASIGLFDYVNLLLYDAPTDTMT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEE
LFSQGDVGGGYARIDGLAPTEGPGQVVWARQRPFVCSGEEYRRHFLKIAALRRNQRVGLY
EEECCCCCCCEEEECCCCCCCCCCCEEEECCCCCEECCHHHHHHHHHHHHHHCCCCCCEE
CQMPLTTSQRRLGGVEFISSRPTTYDEDDLTFLRRVASVIAISVENALIGEMRQMEEERL
EECCCCCCHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGERDHNHILVEVTNAVISKLKLEDLVDEVSRSIHRFFGVDFVSLDLVDPATATIKSRSV
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCHH
YFQKGLGPVWGSAQGPLGASPMAPLFETKAPRILARADLGALARGHDQMTLLTNAGFQVA
HHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCHHHHHCCCCCEEEEECCCCEEE
CGIPLLSAGHVLGVLTLASRERDKFTDYNIGLLGEVASRICIAVENALAYGEISRLKDQL
ECCCHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRENKQLAKERYYLEEEIRRTEDFGDIIGISPAIRGVLEQVELVADSDSTVLILGETGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCC
KELIARAIHMLSQRKKRTMIKVNCAAIPSALLESDLFGHEKGAFTGAIAQKSGRFELADR
HHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCCCCEEHHHHCCCCCEEECCC
GTLFLDEVGDIPIDLQPKLLRALQEREIERVGGHQPIPVDVRMIAATNRDLTGMVEKRLF
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHH
RGDLYYRLNVFPILIPPLRERREDIPLMADYFMRKTARKMNRAIEAFSAETMDMLLNLPW
HCCEEEEEEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
PGNVRELQNVIERAVILTKGPVLHLPATELRHHLAPSAALPSEDAVIPLRAAVLPSAASA
CCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCCCCCCCCCCEEEEHHHHCCCCCCC
PDPLPPPAGSSPNPGDLAGEAIERDQILRVLRETNGIVGGPRGAAAHLGMKRTTLLSRMR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHH
RLGISPKEVDHRA
HCCCCHHHCCCCC
>Mature Secondary Structure 
GEAHSLAERYELLLDVSEILELNRDLNSLLGALCDLPASIGLFDYVNLLLYDAPTDTMT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEE
LFSQGDVGGGYARIDGLAPTEGPGQVVWARQRPFVCSGEEYRRHFLKIAALRRNQRVGLY
EEECCCCCCCEEEECCCCCCCCCCCEEEECCCCCEECCHHHHHHHHHHHHHHCCCCCCEE
CQMPLTTSQRRLGGVEFISSRPTTYDEDDLTFLRRVASVIAISVENALIGEMRQMEEERL
EECCCCCCHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGERDHNHILVEVTNAVISKLKLEDLVDEVSRSIHRFFGVDFVSLDLVDPATATIKSRSV
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCHH
YFQKGLGPVWGSAQGPLGASPMAPLFETKAPRILARADLGALARGHDQMTLLTNAGFQVA
HHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCHHHHHCCCCCEEEEECCCCEEE
CGIPLLSAGHVLGVLTLASRERDKFTDYNIGLLGEVASRICIAVENALAYGEISRLKDQL
ECCCHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRENKQLAKERYYLEEEIRRTEDFGDIIGISPAIRGVLEQVELVADSDSTVLILGETGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCC
KELIARAIHMLSQRKKRTMIKVNCAAIPSALLESDLFGHEKGAFTGAIAQKSGRFELADR
HHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCCCCEEHHHHCCCCCEEECCC
GTLFLDEVGDIPIDLQPKLLRALQEREIERVGGHQPIPVDVRMIAATNRDLTGMVEKRLF
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHH
RGDLYYRLNVFPILIPPLRERREDIPLMADYFMRKTARKMNRAIEAFSAETMDMLLNLPW
HCCEEEEEEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
PGNVRELQNVIERAVILTKGPVLHLPATELRHHLAPSAALPSEDAVIPLRAAVLPSAASA
CCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCCCCCCCCCCEEEEHHHHCCCCCCC
PDPLPPPAGSSPNPGDLAGEAIERDQILRVLRETNGIVGGPRGAAAHLGMKRTTLLSRMR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHH
RLGISPKEVDHRA
HCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]