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The map label for this gene is mcl1 [H]

Identifier: 83591557

GI number: 83591557

Start: 268873

End: 269850

Strand: Direct

Name: mcl1 [H]

Synonym: Rru_A0217

Alternate gene names: 83591557

Gene position: 268873-269850 (Clockwise)

Preceding gene: 83591556

Following gene: 83591558

Centisome position: 6.18

GC content: 64.21

Gene sequence:

>978_bases
ATGAGCTTCACTCTTGTCGATCAGGCGCCGGCCCGTCTCAACCGTTCGGAACAGGCCGTGCCGGGCTCCAACACCAAATT
CCTGGAAAAGGCCGCCAAGGGCGATGCCGACGTGGTGTTCCTCGATCTGGAGGACGCGGTGGCGCCCTCGGAAAAGGAAC
AGGCGCGCAAGAACATCATCGCCGCGCTCAATGACCTGGACTGGTCGGGCAAGACCGTGTCGCTGCGCATCAACGGCCTT
GATACCCATTACATGTACCGCGATGTCATCGACGTGCTGGAACAGGCGCCGGGCAAGCTTGATCTGATCATGATCCCCAA
GGCCGGCACGGCGGCCGACGTCTATGCCCTTGATATGCTGGTCACCCAGATCGAAACGGCGACCAAGGTCGACAAGCGCA
TCGGCTTCGAGCTGATCATCGAAACCGCCTTGGGCATGCGCAATATCCACGAGATCGCCGGGGCCAGCCCGCGCAACGAG
TCGTTGCATTTCGGCGTGGCCGATTACGCCGCCTCGACCAAGGCGCGGACGACGGCGATCGGCGGCCCCAACGCCGATTA
CGGCGTGCTGACCGATCCGCTTGACGATGGCCAGCGGGCCTATCATTGGGGGGACATGTGGCATTACGCCATCGCCCGCA
TGGTGGTCGCCGCCCGCGCCCACGGCCTGCGCCCGGTTGACGGCCCCTTTGGCGATATCGGCGATCACGACGGCTATCGC
GCCCAGGCCTTGCGCGCCGCCGTGCTGGGCTGCGAGGGCAAATGGGCGATCCATCCCAGTCAGGTGACGTTGGCCAACGA
GGTGTTCAGCCCCTCGGACAAGGAGGTCGCCCATGCCCAGGCCATTCTTGACGCCATGGAACAGGCGGGCAAAGAGGGCA
AGGGGGCGGTTACTCTCAACGGCCGGCTGATCGATATCGCCTCGATCAAGCAGGCCCAGGTGATGGTCGACAAGGCCCGT
CAGGTCCGCGCCGGCTAA

Upstream 100 bases:

>100_bases
CGGTCCGCAACCCATTCTTGCCAACGGACGCGCCCGCCAACCGGCCGTCTGGCGCCGACGCCGCCGTTGGCGCTCCAATC
ACCAGGAACAGGGAATCCCC

Downstream 100 bases:

>100_bases
AAGGCCCCCCGGGGTGGATCGCCAAAAAGGCGGCGATCCACCCTTGGCGCGTGTCCAACCATCCGGAAATGATATCCCTG
TTTGGCTCCGGCCCGGCCTT

Product: citrate lyase

Products: NA

Alternate protein names: (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; L-malyl-CoA/beta-methylmalyl-CoA lyase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSFTLVDQAPARLNRSEQAVPGSNTKFLEKAAKGDADVVFLDLEDAVAPSEKEQARKNIIAALNDLDWSGKTVSLRINGL
DTHYMYRDVIDVLEQAPGKLDLIMIPKAGTAADVYALDMLVTQIETATKVDKRIGFELIIETALGMRNIHEIAGASPRNE
SLHFGVADYAASTKARTTAIGGPNADYGVLTDPLDDGQRAYHWGDMWHYAIARMVVAARAHGLRPVDGPFGDIGDHDGYR
AQALRAAVLGCEGKWAIHPSQVTLANEVFSPSDKEVAHAQAILDAMEQAGKEGKGAVTLNGRLIDIASIKQAQVMVDKAR
QVRAG

Sequences:

>Translated_325_residues
MSFTLVDQAPARLNRSEQAVPGSNTKFLEKAAKGDADVVFLDLEDAVAPSEKEQARKNIIAALNDLDWSGKTVSLRINGL
DTHYMYRDVIDVLEQAPGKLDLIMIPKAGTAADVYALDMLVTQIETATKVDKRIGFELIIETALGMRNIHEIAGASPRNE
SLHFGVADYAASTKARTTAIGGPNADYGVLTDPLDDGQRAYHWGDMWHYAIARMVVAARAHGLRPVDGPFGDIGDHDGYR
AQALRAAVLGCEGKWAIHPSQVTLANEVFSPSDKEVAHAQAILDAMEQAGKEGKGAVTLNGRLIDIASIKQAQVMVDKAR
QVRAG
>Mature_324_residues
SFTLVDQAPARLNRSEQAVPGSNTKFLEKAAKGDADVVFLDLEDAVAPSEKEQARKNIIAALNDLDWSGKTVSLRINGLD
THYMYRDVIDVLEQAPGKLDLIMIPKAGTAADVYALDMLVTQIETATKVDKRIGFELIIETALGMRNIHEIAGASPRNES
LHFGVADYAASTKARTTAIGGPNADYGVLTDPLDDGQRAYHWGDMWHYAIARMVVAARAHGLRPVDGPFGDIGDHDGYRA
QALRAAVLGCEGKWAIHPSQVTLANEVFSPSDKEVAHAQAILDAMEQAGKEGKGAVTLNGRLIDIASIKQAQVMVDKARQ
VRAG

Specific function: Catalyzes the reversible condensation of glyoxylate and acetyl-CoA to L-malyl-CoA and the reversible condensation of glyoxylate and propionyl-CoA to beta-methylmalyl-CoA [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=315, Percent_Identity=26.984126984127, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI87081764, Length=315, Percent_Identity=29.5238095238095, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17557458, Length=320, Percent_Identity=28.4375, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.24 [H]

Molecular weight: Translated: 35053; Mature: 34922

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTLVDQAPARLNRSEQAVPGSNTKFLEKAAKGDADVVFLDLEDAVAPSEKEQARKNII
CCEEECCCCHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECHHCCCCCHHHHHHHHHH
AALNDLDWSGKTVSLRINGLDTHYMYRDVIDVLEQAPGKLDLIMIPKAGTAADVYALDML
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
VTQIETATKVDKRIGFELIIETALGMRNIHEIAGASPRNESLHFGVADYAASTKARTTAI
HHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHCCCCCCCCEEECHHHHHCCCCCCEEEC
GGPNADYGVLTDPLDDGQRAYHWGDMWHYAIARMVVAARAHGLRPVDGPFGDIGDHDGYR
CCCCCCCCCEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
AQALRAAVLGCEGKWAIHPSQVTLANEVFSPSDKEVAHAQAILDAMEQAGKEGKGAVTLN
HHHHHHHHHCCCCCEEECCCCEEEHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEC
GRLIDIASIKQAQVMVDKARQVRAG
CEEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFTLVDQAPARLNRSEQAVPGSNTKFLEKAAKGDADVVFLDLEDAVAPSEKEQARKNII
CEEECCCCHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECHHCCCCCHHHHHHHHHH
AALNDLDWSGKTVSLRINGLDTHYMYRDVIDVLEQAPGKLDLIMIPKAGTAADVYALDML
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
VTQIETATKVDKRIGFELIIETALGMRNIHEIAGASPRNESLHFGVADYAASTKARTTAI
HHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHCCCCCCCCEEECHHHHHCCCCCCEEEC
GGPNADYGVLTDPLDDGQRAYHWGDMWHYAIARMVVAARAHGLRPVDGPFGDIGDHDGYR
CCCCCCCCCEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
AQALRAAVLGCEGKWAIHPSQVTLANEVFSPSDKEVAHAQAILDAMEQAGKEGKGAVTLN
HHHHHHHHHCCCCCEEECCCCEEEHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEC
GRLIDIASIKQAQVMVDKARQVRAG
CEEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA