Click here to switch to the map view.

The map label for this gene is leuC

Identifier: 83591075

GI number: 83591075

Start: 2357722

End: 2358987

Strand: Reverse

Name: leuC

Synonym: Moth_2254

Alternate gene names: 83591075

Gene position: 2358987-2357722 (Counterclockwise)

Preceding gene: 83591076

Following gene: 83591074

Centisome position: 89.74

GC content: 62.16

Gene sequence:

>1266_bases
ATGGGCATGACCATCACCGAAAAGATCCTGGCCGCCCACGCCGGATTAAAGGCGGTAGAGCCTGGCCAGCTCATCAACGC
TAAAGTAGACCTGGCCCTGGGGAACGATATCACCGCCCCCCTGGCCATCCAGGAGTTCAAAAAGCTCGGAGTGAAAAAAG
TCTTCGACCCGGAGCGGGTCGTCCTGGTACCGGACCACTTCACCCCGGCCAAGGACATTAAGTCCGCCGAGCAGGCGAAA
ATCTTGCGCGATTTCGCCCGGGAGCAGGGCTTGACCCACTACTTTGAAATCGGCCGTATGGGCATCGAGCACTGCCTGCT
GCCGGAGGCGGGCCTGGTCGGTCCCGGAGACCTGGTCATCGGTGCCGACTCCCATACCTGCACCTACGGGGCCCTCGGGG
CCTTCGCCACCGGCGTCGGCTCCACGGACCTCGCCGCCGCCATGGCCACCGGCGAGCTATGGTTTAAAGTACCCGAGACC
ATTCTCTTCCGTTACCACGGGAAGCTTAAGCCCTGGGTAGGCGGCAAAGACCTCATCCTCTACACCATCGGCCGGATCGG
CGTCGACGGCGCCCGCTATATGGCCATGGAATTCACCGGCGAAGCCATTACCAACCTCTCCATGGAAGGCCGCTTCACCA
TGGCCAACATGGCCATCGAAGCCGGCGGCAAGAACGGCATCTTCCCGGTGGACGAAAAGACCGTAGAATACATCCGGGGC
CGGCTGCAAAGGGACTACCGCATCTACCAGAGCGACCCCGACGCCCGTTACAATCAGGAGATAGACATCGACGCCAGTAA
GATCGAACCCCAGGTAGCCCTGCCCCACCTGCCCGAAAACGCCCGGAGTGTAAAAGAAATAGGAGAGATCAAAATCGACC
AGGTAGTCATCGGCAGCTGCACCAACGGCCGCCTGGAGGACCTGCGGGTGGCGGCGCAAATCCTAAAGGGGCAAAAGGTC
CATCCCGAAGTAAGACTCATTGTCATCCCCGGCACCCAGCAGATCTACGCCGCAGCCCTGGCCGAAGGGTTGATAGCAAC
CTTTATCGAAGCCGGGGCGGCCGTATCCACCCCCACCTGCGGCCCCTGCCTGGGCGGGCATATGGGGATCCTGGCTAAAG
GGGAGCGGGCCCTGGCCACCACCAACCGCAACTTCGTCGGCCGCATGGGCCATCCCGAGAGCGAAGTCTATCTCGCCGGC
CCGGCGGTAGCCGCCGCCAGCGCCGTTAAAGGGCGCATCGCCGCCCCAGAGGAGGTAGTAAAATGA

Upstream 100 bases:

>100_bases
TGGCTCCTGAAGGAAACATGCCTCACCCGAACCTGTGAGCAGGTTAAACAGATGCGGGACATATCCCGGATAGAAGTATT
TGTGAGAGCGAGGAATAGCC

Downstream 100 bases:

>100_bases
TCATTCAGGGCAAGTGCCACACCTTCGGCAACGACATCGACACCGACGCCATCATCCCGGCGCGGTATTTAAATACCACC
GACCCCGGGGAACTGGCCCG

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MGMTITEKILAAHAGLKAVEPGQLINAKVDLALGNDITAPLAIQEFKKLGVKKVFDPERVVLVPDHFTPAKDIKSAEQAK
ILRDFAREQGLTHYFEIGRMGIEHCLLPEAGLVGPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMATGELWFKVPET
ILFRYHGKLKPWVGGKDLILYTIGRIGVDGARYMAMEFTGEAITNLSMEGRFTMANMAIEAGGKNGIFPVDEKTVEYIRG
RLQRDYRIYQSDPDARYNQEIDIDASKIEPQVALPHLPENARSVKEIGEIKIDQVVIGSCTNGRLEDLRVAAQILKGQKV
HPEVRLIVIPGTQQIYAAALAEGLIATFIEAGAAVSTPTCGPCLGGHMGILAKGERALATTNRNFVGRMGHPESEVYLAG
PAVAAASAVKGRIAAPEEVVK

Sequences:

>Translated_421_residues
MGMTITEKILAAHAGLKAVEPGQLINAKVDLALGNDITAPLAIQEFKKLGVKKVFDPERVVLVPDHFTPAKDIKSAEQAK
ILRDFAREQGLTHYFEIGRMGIEHCLLPEAGLVGPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMATGELWFKVPET
ILFRYHGKLKPWVGGKDLILYTIGRIGVDGARYMAMEFTGEAITNLSMEGRFTMANMAIEAGGKNGIFPVDEKTVEYIRG
RLQRDYRIYQSDPDARYNQEIDIDASKIEPQVALPHLPENARSVKEIGEIKIDQVVIGSCTNGRLEDLRVAAQILKGQKV
HPEVRLIVIPGTQQIYAAALAEGLIATFIEAGAAVSTPTCGPCLGGHMGILAKGERALATTNRNFVGRMGHPESEVYLAG
PAVAAASAVKGRIAAPEEVVK
>Mature_420_residues
GMTITEKILAAHAGLKAVEPGQLINAKVDLALGNDITAPLAIQEFKKLGVKKVFDPERVVLVPDHFTPAKDIKSAEQAKI
LRDFAREQGLTHYFEIGRMGIEHCLLPEAGLVGPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMATGELWFKVPETI
LFRYHGKLKPWVGGKDLILYTIGRIGVDGARYMAMEFTGEAITNLSMEGRFTMANMAIEAGGKNGIFPVDEKTVEYIRGR
LQRDYRIYQSDPDARYNQEIDIDASKIEPQVALPHLPENARSVKEIGEIKIDQVVIGSCTNGRLEDLRVAAQILKGQKVH
PEVRLIVIPGTQQIYAAALAEGLIATFIEAGAAVSTPTCGPCLGGHMGILAKGERALATTNRNFVGRMGHPESEVYLAGP
AVAAASAVKGRIAAPEEVVK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=447, Percent_Identity=29.3064876957494, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI8659555, Length=371, Percent_Identity=25.0673854447439, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI41352693, Length=395, Percent_Identity=23.2911392405063, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=35.6837606837607, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI87081781, Length=335, Percent_Identity=28.0597014925373, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI2367097, Length=464, Percent_Identity=26.5086206896552, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=25.9668508287293, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=447, Percent_Identity=29.7539149888143, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32564738, Length=410, Percent_Identity=30.2439024390244, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25149342, Length=298, Percent_Identity=29.8657718120805, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17568399, Length=370, Percent_Identity=26.2162162162162, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=33.9246119733925, Blast_Score=232, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6321429, Length=444, Percent_Identity=34.4594594594595, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6323335, Length=449, Percent_Identity=30.9576837416481, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6322261, Length=459, Percent_Identity=28.9760348583878, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI281365315, Length=448, Percent_Identity=29.2410714285714, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17864292, Length=448, Percent_Identity=29.2410714285714, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI28571643, Length=446, Percent_Identity=29.8206278026906, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI161076999, Length=411, Percent_Identity=29.9270072992701, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17137564, Length=381, Percent_Identity=25.9842519685039, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24645686, Length=375, Percent_Identity=26.4, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_MOOTA (Q2RG98)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_431084.1
- ProteinModelPortal:   Q2RG98
- SMR:   Q2RG98
- STRING:   Q2RG98
- GeneID:   3830749
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2254
- NMPDR:   fig|264732.9.peg.2285
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   TDPRDFL
- PhylomeDB:   Q2RG98
- ProtClustDB:   PRK00402
- BioCyc:   MTHE264732:MOTH_2254-MONOMER
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086
- TIGRFAMs:   TIGR02083

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 45150; Mature: 45019

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMTITEKILAAHAGLKAVEPGQLINAKVDLALGNDITAPLAIQEFKKLGVKKVFDPERV
CCCCHHHHHHHHHCCCCCCCCCCEECEEEEEEECCCCCCCHHHHHHHHCCCHHCCCCCEE
VLVPDHFTPAKDIKSAEQAKILRDFAREQGLTHYFEIGRMGIEHCLLPEAGLVGPGDLVI
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
GADSHTCTYGALGAFATGVGSTDLAAAMATGELWFKVPETILFRYHGKLKPWVGGKDLIL
ECCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHEEECCHHHEEEECCCCCCCCCCCEEEE
YTIGRIGVDGARYMAMEFTGEAITNLSMEGRFTMANMAIEAGGKNGIFPVDEKTVEYIRG
EEECCCCCCCCEEEEEEECCHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHH
RLQRDYRIYQSDPDARYNQEIDIDASKIEPQVALPHLPENARSVKEIGEIKIDQVVIGSC
HHHHCEEEECCCCCCCCCCEECCCHHHCCCCEECCCCCCHHHHHHHHHCEEEEEEEEECC
TNGRLEDLRVAAQILKGQKVHPEVRLIVIPGTQQIYAAALAEGLIATFIEAGAAVSTPTC
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPCLGGHMGILAKGERALATTNRNFVGRMGHPESEVYLAGPAVAAASAVKGRIAAPEEVV
CCCCCCCCCEEECCCEEEEECCCCCEECCCCCCCCEEEECCHHHHHHHHCCCCCCHHHHC
K
C
>Mature Secondary Structure 
GMTITEKILAAHAGLKAVEPGQLINAKVDLALGNDITAPLAIQEFKKLGVKKVFDPERV
CCCHHHHHHHHHCCCCCCCCCCEECEEEEEEECCCCCCCHHHHHHHHCCCHHCCCCCEE
VLVPDHFTPAKDIKSAEQAKILRDFAREQGLTHYFEIGRMGIEHCLLPEAGLVGPGDLVI
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
GADSHTCTYGALGAFATGVGSTDLAAAMATGELWFKVPETILFRYHGKLKPWVGGKDLIL
ECCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHEEECCHHHEEEECCCCCCCCCCCEEEE
YTIGRIGVDGARYMAMEFTGEAITNLSMEGRFTMANMAIEAGGKNGIFPVDEKTVEYIRG
EEECCCCCCCCEEEEEEECCHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHH
RLQRDYRIYQSDPDARYNQEIDIDASKIEPQVALPHLPENARSVKEIGEIKIDQVVIGSC
HHHHCEEEECCCCCCCCCCEECCCHHHCCCCEECCCCCCHHHHHHHHHCEEEEEEEEECC
TNGRLEDLRVAAQILKGQKVHPEVRLIVIPGTQQIYAAALAEGLIATFIEAGAAVSTPTC
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPCLGGHMGILAKGERALATTNRNFVGRMGHPESEVYLAGPAVAAASAVKGRIAAPEEVV
CCCCCCCCCEEECCCEEEEECCCCCEECCCCCCCCEEEECCHHHHHHHHCCCCCCHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA