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The map label for this gene is hyfB [H]

Identifier: 83591014

GI number: 83591014

Start: 2289914

End: 2291935

Strand: Reverse

Name: hyfB [H]

Synonym: Moth_2191

Alternate gene names: 83591014

Gene position: 2291935-2289914 (Counterclockwise)

Preceding gene: 83591015

Following gene: 83591013

Centisome position: 87.19

GC content: 52.08

Gene sequence:

>2022_bases
ATGCTTACGCAACAGCTTCTGCTACTCTCTGTGCTCTTGTACGTTGCCGGAGCCCTTGCCTCCCTGGCTCTTAATCGGGC
CGGTAAAATTGCCAACTATGCTTCAGGTATAAGTGCCCTTGCAGCAGCAGGTACCGGGATGGCCTCGGCCGTCCAGGTAC
TTGCCGGCGGAGCAGCTTTTACCTGGGAAGCGGCGGGGTTTATACCCTTTGCCAAGTTTATTATAAAGGTTGATCCCCTC
TCTGCCTTTATGTTACTGGTTATTTCCCTTCTGACAGGGGCTACGGCTCTATATTCCCTCTCGTACCTGGATGAGTATAC
CGGTAAAGGCGCAGGGGTTATGGGTTTTTTCAATAACCTCTTTATTGCCTCTATGGTATTAGTGGTCATTAGTGGGAATG
CTTTTTATTTTCTAATTTTCTGGGAACTGATGACGCTGGCCTCTTATTTCCTGGTTAGCTTTGATCAGGAAGACAGTGAA
GCTGTCAAGGCCGGGTTCATCTATCTTTTTATGGCCCACGCGGGAACGGCTTTGATTATGCTGGCTTTTATCTTATTCTT
TGTCTATACAGGTACCTTCGATTTCGCTTCCTTCCGTGGGGCGAACCTCCCGGTGTTTACAAAGAGCTTGATCTTCCTGC
TAGCTTTCCTGGGATTCGGGGCCAAGGCCGGTATTATTCCGCTCCATATCTGGCTGCCGAAGGCTCACCCGGCTGCTCCG
TCCAACGCTTCCGCTCTCATGTCGGGTGTCATGATTAAAACCGCTATCTATGGTATTCTCAGGGTCAGTGTCGATTTCCT
GGGGGCTTCTGTTTGGTGGTGGGGATTTATTGTCCTGGCCTCCGGAGCGATTTCAGCAGTTCTGGGTGTTCTCTACGCCC
TGGGGGAGCACGATATAAAGCGGCTGCTGGCCTATCACAGTGTTGAAAACGTTGGGATTATATTGATGGGAGCCGGCGCC
GGCATGATCGGCATCGCTGCCGGCCAGCCTGTTTTAGGAGTACTCGGGATCCTGGCAGGCCTCTACCACTTGTTAAACCA
TGCCGTCTTTAAAGGCTTGCTCTTTCTTGGGGCAGGTTCGGTAATATATCGAACCCATACGAAACATATGGAGGAACTTG
GCGGACTGGCCAGGCGCATGCCCTGGACGGCACTCGCTTTTCTGGTTGGTGCTGTAGCCATCTCAGCCATCCCTCCCCTC
AACGGATTTGTCAGTGAGTGGTTTACCTACCAGTCGTTGTTTATTGCGAGCACCAGCAGCATCCTGGCTGTGAGAGTGTT
TGCGCCCCTGTTTGTTGTTATGTTAGCCCTGACGGGCGCGCTGGCGGCGATGTGTTTTGTGAAGGCATATGGGGTTACTT
TTGGCGGTCCCTGTCGCAGCGGGCATGCCCGTGAGGCCAGGGAGGTTCCCATACCGATGCTTGCCGGGATGGCAATTCTG
GCAATTAGCTGCATTATCCTCGGTGTGGGTGCACCGGTTGTTGCTCCTTATATTGGAAAGGTGGCTTCGGCATTATTAGC
CATTACTGCGGTCCAGGTAAGCGACGGTTTGCTGGTATTTCCTGCAAACAGCATGCAGGCCATGCTCTCCACACCGCTCA
TCGCCATTCTCCTCGTTGGTCTTGCTACGTTACCCTTGTTAATTATCGGGATCCAAGGTGGTTTCCAGGCCGGACGGCGT
ATCGATGCTGAGCCGTGGGCATGTGGATACAAGTATTCACCGCGGATGGCCTATACCGCAACTGCCTTTGCTCAGCCGTT
GCGCGTACTTTTTCGGCCGGTTTATTCGCTCAGGACCACCCTCGATGGACCTGGCTATACCGTTGCATCGTATTTCAAAG
GAGCAGTGGTCTACATCGCCAGTGTAGAATCGTTGTGGGAACGTTACATTTACGCTCCTCTGGCACGGGGCACGGTATAT
CTGGGTAAAAAATTGCAGGCCTTCCAGATAGGGAACGTCAGGCTATATTGTCTCTATATAATCATAACCCTCGTAGTCCT
GTTATTAGCGACGGTTAGATAG

Upstream 100 bases:

>100_bases
GTCTCGTTGAAGGTGAAGAAATTGAACGGCTGAGCAAGTTAAAGAGGCGTACCAGTATTGTGGGCGTGACCTCCATCTTA
GCAGAAGGGAGAAAAAGTTG

Downstream 100 bases:

>100_bases
GAGGAGAAAGAAATGCCCGGTATCAAATTGTTCCTCGTCGGCCTGATGCAGGCCCTGTTAGTCCTGCTGGTAGCACCTCT
TTTCGCGGGCCTTACCCGTG

Product: hydrogenase 4 subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MLTQQLLLLSVLLYVAGALASLALNRAGKIANYASGISALAAAGTGMASAVQVLAGGAAFTWEAAGFIPFAKFIIKVDPL
SAFMLLVISLLTGATALYSLSYLDEYTGKGAGVMGFFNNLFIASMVLVVISGNAFYFLIFWELMTLASYFLVSFDQEDSE
AVKAGFIYLFMAHAGTALIMLAFILFFVYTGTFDFASFRGANLPVFTKSLIFLLAFLGFGAKAGIIPLHIWLPKAHPAAP
SNASALMSGVMIKTAIYGILRVSVDFLGASVWWWGFIVLASGAISAVLGVLYALGEHDIKRLLAYHSVENVGIILMGAGA
GMIGIAAGQPVLGVLGILAGLYHLLNHAVFKGLLFLGAGSVIYRTHTKHMEELGGLARRMPWTALAFLVGAVAISAIPPL
NGFVSEWFTYQSLFIASTSSILAVRVFAPLFVVMLALTGALAAMCFVKAYGVTFGGPCRSGHAREAREVPIPMLAGMAIL
AISCIILGVGAPVVAPYIGKVASALLAITAVQVSDGLLVFPANSMQAMLSTPLIAILLVGLATLPLLIIGIQGGFQAGRR
IDAEPWACGYKYSPRMAYTATAFAQPLRVLFRPVYSLRTTLDGPGYTVASYFKGAVVYIASVESLWERYIYAPLARGTVY
LGKKLQAFQIGNVRLYCLYIIITLVVLLLATVR

Sequences:

>Translated_673_residues
MLTQQLLLLSVLLYVAGALASLALNRAGKIANYASGISALAAAGTGMASAVQVLAGGAAFTWEAAGFIPFAKFIIKVDPL
SAFMLLVISLLTGATALYSLSYLDEYTGKGAGVMGFFNNLFIASMVLVVISGNAFYFLIFWELMTLASYFLVSFDQEDSE
AVKAGFIYLFMAHAGTALIMLAFILFFVYTGTFDFASFRGANLPVFTKSLIFLLAFLGFGAKAGIIPLHIWLPKAHPAAP
SNASALMSGVMIKTAIYGILRVSVDFLGASVWWWGFIVLASGAISAVLGVLYALGEHDIKRLLAYHSVENVGIILMGAGA
GMIGIAAGQPVLGVLGILAGLYHLLNHAVFKGLLFLGAGSVIYRTHTKHMEELGGLARRMPWTALAFLVGAVAISAIPPL
NGFVSEWFTYQSLFIASTSSILAVRVFAPLFVVMLALTGALAAMCFVKAYGVTFGGPCRSGHAREAREVPIPMLAGMAIL
AISCIILGVGAPVVAPYIGKVASALLAITAVQVSDGLLVFPANSMQAMLSTPLIAILLVGLATLPLLIIGIQGGFQAGRR
IDAEPWACGYKYSPRMAYTATAFAQPLRVLFRPVYSLRTTLDGPGYTVASYFKGAVVYIASVESLWERYIYAPLARGTVY
LGKKLQAFQIGNVRLYCLYIIITLVVLLLATVR
>Mature_673_residues
MLTQQLLLLSVLLYVAGALASLALNRAGKIANYASGISALAAAGTGMASAVQVLAGGAAFTWEAAGFIPFAKFIIKVDPL
SAFMLLVISLLTGATALYSLSYLDEYTGKGAGVMGFFNNLFIASMVLVVISGNAFYFLIFWELMTLASYFLVSFDQEDSE
AVKAGFIYLFMAHAGTALIMLAFILFFVYTGTFDFASFRGANLPVFTKSLIFLLAFLGFGAKAGIIPLHIWLPKAHPAAP
SNASALMSGVMIKTAIYGILRVSVDFLGASVWWWGFIVLASGAISAVLGVLYALGEHDIKRLLAYHSVENVGIILMGAGA
GMIGIAAGQPVLGVLGILAGLYHLLNHAVFKGLLFLGAGSVIYRTHTKHMEELGGLARRMPWTALAFLVGAVAISAIPPL
NGFVSEWFTYQSLFIASTSSILAVRVFAPLFVVMLALTGALAAMCFVKAYGVTFGGPCRSGHAREAREVPIPMLAGMAIL
AISCIILGVGAPVVAPYIGKVASALLAITAVQVSDGLLVFPANSMQAMLSTPLIAILLVGLATLPLLIIGIQGGFQAGRR
IDAEPWACGYKYSPRMAYTATAFAQPLRVLFRPVYSLRTTLDGPGYTVASYFKGAVVYIASVESLWERYIYAPLARGTVY
LGKKLQAFQIGNVRLYCLYIIITLVVLLLATVR

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=337, Percent_Identity=28.1899109792285, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1788827, Length=668, Percent_Identity=47.9041916167665, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI2367154, Length=540, Percent_Identity=40.1851851851852, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI1788831, Length=368, Percent_Identity=32.6086956521739, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788613, Length=394, Percent_Identity=29.4416243654822, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1788614, Length=362, Percent_Identity=32.8729281767956, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1788829, Length=433, Percent_Identity=26.0969976905312, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 71497; Mature: 71497

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQQLLLLSVLLYVAGALASLALNRAGKIANYASGISALAAAGTGMASAVQVLAGGAAF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
TWEAAGFIPFAKFIIKVDPLSAFMLLVISLLTGATALYSLSYLDEYTGKGAGVMGFFNNL
EECCCCHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
FIASMVLVVISGNAFYFLIFWELMTLASYFLVSFDQEDSEAVKAGFIYLFMAHAGTALIM
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAFILFFVYTGTFDFASFRGANLPVFTKSLIFLLAFLGFGAKAGIIPLHIWLPKAHPAAP
HHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCC
SNASALMSGVMIKTAIYGILRVSVDFLGASVWWWGFIVLASGAISAVLGVLYALGEHDIK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
RLLAYHSVENVGIILMGAGAGMIGIAAGQPVLGVLGILAGLYHLLNHAVFKGLLFLGAGS
HHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VIYRTHTKHMEELGGLARRMPWTALAFLVGAVAISAIPPLNGFVSEWFTYQSLFIASTSS
CEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHH
ILAVRVFAPLFVVMLALTGALAAMCFVKAYGVTFGGPCRSGHAREAREVPIPMLAGMAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHHHHHHH
AISCIILGVGAPVVAPYIGKVASALLAITAVQVSDGLLVFPANSMQAMLSTPLIAILLVG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCHHHHHHHHHHHHHHHHH
LATLPLLIIGIQGGFQAGRRIDAEPWACGYKYSPRMAYTATAFAQPLRVLFRPVYSLRTT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
LDGPGYTVASYFKGAVVYIASVESLWERYIYAPLARGTVYLGKKLQAFQIGNVRLYCLYI
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEHHCCCCCEEEECCCHHHHHHH
IITLVVLLLATVR
HHHHHHHHHHHCC
>Mature Secondary Structure
MLTQQLLLLSVLLYVAGALASLALNRAGKIANYASGISALAAAGTGMASAVQVLAGGAAF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
TWEAAGFIPFAKFIIKVDPLSAFMLLVISLLTGATALYSLSYLDEYTGKGAGVMGFFNNL
EECCCCHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
FIASMVLVVISGNAFYFLIFWELMTLASYFLVSFDQEDSEAVKAGFIYLFMAHAGTALIM
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAFILFFVYTGTFDFASFRGANLPVFTKSLIFLLAFLGFGAKAGIIPLHIWLPKAHPAAP
HHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCC
SNASALMSGVMIKTAIYGILRVSVDFLGASVWWWGFIVLASGAISAVLGVLYALGEHDIK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
RLLAYHSVENVGIILMGAGAGMIGIAAGQPVLGVLGILAGLYHLLNHAVFKGLLFLGAGS
HHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VIYRTHTKHMEELGGLARRMPWTALAFLVGAVAISAIPPLNGFVSEWFTYQSLFIASTSS
CEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHH
ILAVRVFAPLFVVMLALTGALAAMCFVKAYGVTFGGPCRSGHAREAREVPIPMLAGMAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHHHHHHH
AISCIILGVGAPVVAPYIGKVASALLAITAVQVSDGLLVFPANSMQAMLSTPLIAILLVG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCHHHHHHHHHHHHHHHHH
LATLPLLIIGIQGGFQAGRRIDAEPWACGYKYSPRMAYTATAFAQPLRVLFRPVYSLRTT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
LDGPGYTVASYFKGAVVYIASVESLWERYIYAPLARGTVYLGKKLQAFQIGNVRLYCLYI
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEHHCCCCCEEEECCCHHHHHHH
IITLVVLLLATVR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]