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The map label for this gene is hyfA [H]

Identifier: 83591015

GI number: 83591015

Start: 2291935

End: 2292504

Strand: Reverse

Name: hyfA [H]

Synonym: Moth_2192

Alternate gene names: 83591015

Gene position: 2292504-2291935 (Counterclockwise)

Preceding gene: 83591017

Following gene: 83591014

Centisome position: 87.21

GC content: 51.4

Gene sequence:

>570_bases
ATGCCTAACCGTTTTGTAATTGCCGATCCCGAACGTTGCTTGGGTTGTTATACCTGTATGGCAGGATGCGCTCTTGCCCA
TAACAAGCAGGGGCTACAACCCTTCCCGAGACTGTTTGTTACCCACACCCCGGATGGTACCATGCCCGTCCAGTGCCGCC
ATTGCGAGGACGCTCCCTGCGCCAGGGTATGTCCTGTTAAGGCTATTGAAATTAAGAACCAGATGGTTTATTTAAATGAG
GGCCTCTGTATCGGTTGCAAGATGTGTGCCCTGGTCTGCCCTTTTGGCTGCATCGACATTCAGGGGACACCGGCTCCCTC
GGTCGAAAAAGATGGTCTGCTGAACTCTTATTTGATACCTCTACTGGCCGGGGCTACAGGGCAGAAAACCATTGCTACCA
AGTGTGATCTCTGTTATTTCGATGCTGAGGGTCCCGCCTGTGTAAGAGTTTGTCCGACGCGAGCTTTGCGTCTCGTTGAA
GGTGAAGAAATTGAACGGCTGAGCAAGTTAAAGAGGCGTACCAGTATTGTGGGCGTGACCTCCATCTTAGCAGAAGGGAG
AAAAAGTTGA

Upstream 100 bases:

>100_bases
GAAGCCCGAGCTATTTATCTAGAGAAGAAGGCCAAATACCTCGTTGATCTGGAAAAAGCTAACGTACCAAAAACGGTAAT
AGGGGAGGGAGGAAGGGTCG

Downstream 100 bases:

>100_bases
TGCTTACGCAACAGCTTCTGCTACTCTCTGTGCTCTTGTACGTTGCCGGAGCCCTTGCCTCCCTGGCTCTTAATCGGGCC
GGTAAAATTGCCAACTATGC

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MPNRFVIADPERCLGCYTCMAGCALAHNKQGLQPFPRLFVTHTPDGTMPVQCRHCEDAPCARVCPVKAIEIKNQMVYLNE
GLCIGCKMCALVCPFGCIDIQGTPAPSVEKDGLLNSYLIPLLAGATGQKTIATKCDLCYFDAEGPACVRVCPTRALRLVE
GEEIERLSKLKRRTSIVGVTSILAEGRKS

Sequences:

>Translated_189_residues
MPNRFVIADPERCLGCYTCMAGCALAHNKQGLQPFPRLFVTHTPDGTMPVQCRHCEDAPCARVCPVKAIEIKNQMVYLNE
GLCIGCKMCALVCPFGCIDIQGTPAPSVEKDGLLNSYLIPLLAGATGQKTIATKCDLCYFDAEGPACVRVCPTRALRLVE
GEEIERLSKLKRRTSIVGVTSILAEGRKS
>Mature_188_residues
PNRFVIADPERCLGCYTCMAGCALAHNKQGLQPFPRLFVTHTPDGTMPVQCRHCEDAPCARVCPVKAIEIKNQMVYLNEG
LCIGCKMCALVCPFGCIDIQGTPAPSVEKDGLLNSYLIPLLAGATGQKTIATKCDLCYFDAEGPACVRVCPTRALRLVEG
EEIERLSKLKRRTSIVGVTSILAEGRKS

Specific function: Probable electron transfer protein for hydrogenase 4 [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082114, Length=183, Percent_Identity=49.1803278688525, Blast_Score=171, Evalue=3e-44,
Organism=Escherichia coli, GI1789079, Length=183, Percent_Identity=45.9016393442623, Blast_Score=164, Evalue=2e-42,
Organism=Escherichia coli, GI1789067, Length=180, Percent_Identity=37.7777777777778, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1788811, Length=175, Percent_Identity=38.2857142857143, Blast_Score=118, Evalue=2e-28,
Organism=Escherichia coli, GI87082180, Length=193, Percent_Identity=34.1968911917098, Blast_Score=118, Evalue=2e-28,
Organism=Escherichia coli, GI2367245, Length=171, Percent_Identity=37.4269005847953, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI87082179, Length=171, Percent_Identity=38.5964912280702, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787872, Length=176, Percent_Identity=29.5454545454545, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1787122, Length=176, Percent_Identity=29.5454545454545, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 20506; Mature: 20375

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
11.6 %Cys+Met (Translated Protein)
9.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
11.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNRFVIADPERCLGCYTCMAGCALAHNKQGLQPFPRLFVTHTPDGTMPVQCRHCEDAPC
CCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCC
ARVCPVKAIEIKNQMVYLNEGLCIGCKMCALVCPFGCIDIQGTPAPSVEKDGLLNSYLIP
CCCCCCEEEEECCEEEEEECCEEEHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHH
LLAGATGQKTIATKCDLCYFDAEGPACVRVCPTRALRLVEGEEIERLSKLKRRTSIVGVT
HHCCCCCCCEEEEECCEEEEECCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SILAEGRKS
HHHHCCCCC
>Mature Secondary Structure 
PNRFVIADPERCLGCYTCMAGCALAHNKQGLQPFPRLFVTHTPDGTMPVQCRHCEDAPC
CCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCC
ARVCPVKAIEIKNQMVYLNEGLCIGCKMCALVCPFGCIDIQGTPAPSVEKDGLLNSYLIP
CCCCCCEEEEECCEEEEEECCEEEHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHH
LLAGATGQKTIATKCDLCYFDAEGPACVRVCPTRALRLVEGEEIERLSKLKRRTSIVGVT
HHCCCCCCCEEEEECCEEEEECCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SILAEGRKS
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2016588; 9205837; 9278503 [H]