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The map label for this gene is moaA

Identifier: 83590961

GI number: 83590961

Start: 2232314

End: 2233285

Strand: Reverse

Name: moaA

Synonym: Moth_2135

Alternate gene names: 83590961

Gene position: 2233285-2232314 (Counterclockwise)

Preceding gene: 83590962

Following gene: 83590960

Centisome position: 84.96

GC content: 62.45

Gene sequence:

>972_bases
GTGCAGGACACCTTTCAACGCCAGATAAACTACCTGCGCATCGCCATTACCGATCGCTGTAACCTGCGCTGCCGTTATTG
TATGCCGGCCACGGGGGTGCCCTTGAAGGGTCACGAGGATATCCTGCGCCTGGAAGAGATCGCCACCCTGGCCCGGGTAG
CTGCCGGTACTGGTATCAGCCGGATTCGCCTCACCGGGGGCGAGCCCCTGGTCCGGAAAAACGTGGTGACCCTGGTGCGG
GAACTGGCGGCCATTCCCGGCCTGGAGGAGATCTCCCTGACAACCAACGGCATCTTCCTGGGGGCCCTGGCCTTTTCTTT
AAAAGAGGCCGGACTGAAGCGGGTGAATATCAGCCTGGACACCCTGAAGAAGGACCGCTACCGCTATATCACCCGCCGCG
GCAACATCACCAGCGTCTGGCAGGGCATCCGGGCGGCCCTGGCCGCTGGCCTGACGCCGGTTAAACTCAATGTCGTCATT
ACGCGGGGCTTTAACGACGATGAGATCCTGGATTTTGCCCGGCTGGCCAGGGAAGAACCCCTGCATATCCGTTTTATCGA
GCTCATGCCCATTGGTACGGCGGCCGCCTCCGGTACCGCTTATGTGCCGGCGGAGGAGATTAAGGGCCGGATCAGCCGGG
TTTACCCCCTGGAACCCTTCCCGGACCTGGCAACCAACGGGCCGGCAGCCAATTTCAGGCTGGTCGGCGGCCGGGGAAGT
GTGGGATTTATCACCCCCATGTCCAATCACTTCTGTTCCCGCTGTAACCGCCTGCGCCTGACGGCAGACGGCAAGCTCAG
GCCCTGCCTCTACTGGGACGGGGAGATAGATATCAAAGGGCCTTTGCGTGCCGGGGCTCCGGAGACCGAACTGGCGGCTA
TTTTTGCCCGGGCCGTCAGCTTGAAGCCCGCCGAACACCACATGGAGAACGGCTGGCGCCAGCCCCGGGCCATGTCCCAG
ATAGGCGGCTGA

Upstream 100 bases:

>100_bases
CAGGGAATGCGTTAGCAGGGAGGCCCCGGGCGGTATTTGTCTAGAGGCAGGACCATGGAGCCCGGGCAAGGATCATTTCC
GGCAGAAAGAGGGAAAACCG

Downstream 100 bases:

>100_bases
AACGTGGGAAGTGAGAGGGAGGAACTTCCGCTATGAGCAATCCTCTTACCCACCTCGATACAAAAGGGCAGGCGCGCATG
GTTGACGTCGGCGCCAAGGC

Product: GTP cyclohydrolase subunit MoaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MQDTFQRQINYLRIAITDRCNLRCRYCMPATGVPLKGHEDILRLEEIATLARVAAGTGISRIRLTGGEPLVRKNVVTLVR
ELAAIPGLEEISLTTNGIFLGALAFSLKEAGLKRVNISLDTLKKDRYRYITRRGNITSVWQGIRAALAAGLTPVKLNVVI
TRGFNDDEILDFARLAREEPLHIRFIELMPIGTAAASGTAYVPAEEIKGRISRVYPLEPFPDLATNGPAANFRLVGGRGS
VGFITPMSNHFCSRCNRLRLTADGKLRPCLYWDGEIDIKGPLRAGAPETELAAIFARAVSLKPAEHHMENGWRQPRAMSQ
IGG

Sequences:

>Translated_323_residues
MQDTFQRQINYLRIAITDRCNLRCRYCMPATGVPLKGHEDILRLEEIATLARVAAGTGISRIRLTGGEPLVRKNVVTLVR
ELAAIPGLEEISLTTNGIFLGALAFSLKEAGLKRVNISLDTLKKDRYRYITRRGNITSVWQGIRAALAAGLTPVKLNVVI
TRGFNDDEILDFARLAREEPLHIRFIELMPIGTAAASGTAYVPAEEIKGRISRVYPLEPFPDLATNGPAANFRLVGGRGS
VGFITPMSNHFCSRCNRLRLTADGKLRPCLYWDGEIDIKGPLRAGAPETELAAIFARAVSLKPAEHHMENGWRQPRAMSQ
IGG
>Mature_323_residues
MQDTFQRQINYLRIAITDRCNLRCRYCMPATGVPLKGHEDILRLEEIATLARVAAGTGISRIRLTGGEPLVRKNVVTLVR
ELAAIPGLEEISLTTNGIFLGALAFSLKEAGLKRVNISLDTLKKDRYRYITRRGNITSVWQGIRAALAAGLTPVKLNVVI
TRGFNDDEILDFARLAREEPLHIRFIELMPIGTAAASGTAYVPAEEIKGRISRVYPLEPFPDLATNGPAANFRLVGGRGS
VGFITPMSNHFCSRCNRLRLTADGKLRPCLYWDGEIDIKGPLRAGAPETELAAIFARAVSLKPAEHHMENGWRQPRAMSQ
IGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI22027536, Length=307, Percent_Identity=46.2540716612378, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI116734668, Length=307, Percent_Identity=46.2540716612378, Blast_Score=276, Evalue=3e-74,
Organism=Escherichia coli, GI1786999, Length=336, Percent_Identity=32.7380952380952, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI32566331, Length=310, Percent_Identity=39.3548387096774, Blast_Score=254, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI32566329, Length=329, Percent_Identity=38.6018237082067, Blast_Score=253, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI17561210, Length=326, Percent_Identity=39.2638036809816, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI28574490, Length=312, Percent_Identity=43.5897435897436, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI28574488, Length=336, Percent_Identity=42.8571428571429, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_MOOTA (Q2RGL2)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430970.1
- HSSP:   P69848
- ProteinModelPortal:   Q2RGL2
- SMR:   Q2RGL2
- STRING:   Q2RGL2
- GeneID:   3833135
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2135
- NMPDR:   fig|264732.9.peg.2157
- eggNOG:   COG2896
- HOGENOM:   HBG529885
- OMA:   PMARHFR
- PhylomeDB:   Q2RGL2
- BioCyc:   MTHE264732:MOTH_2135-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 35585; Mature: 35585

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 13-13 BINDING 26-26 BINDING 63-63 BINDING 67-67 BINDING 94-94 BINDING 118-118 BINDING 155-155 BINDING 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDTFQRQINYLRIAITDRCNLRCRYCMPATGVPLKGHEDILRLEEIATLARVAAGTGIS
CCCHHHHHCCEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
RIRLTGGEPLVRKNVVTLVRELAAIPGLEEISLTTNGIFLGALAFSLKEAGLKRVNISLD
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEEHH
TLKKDRYRYITRRGNITSVWQGIRAALAAGLTPVKLNVVITRGFNDDEILDFARLAREEP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCC
LHIRFIELMPIGTAAASGTAYVPAEEIKGRISRVYPLEPFPDLATNGPAANFRLVGGRGS
CEEEEEEEEECCCCCCCCCEECCHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEECCCCC
VGFITPMSNHFCSRCNRLRLTADGKLRPCLYWDGEIDIKGPLRAGAPETELAAIFARAVS
EEEECCCHHHHHHHCCEEEEEECCCCCEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHC
LKPAEHHMENGWRQPRAMSQIGG
CCCHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MQDTFQRQINYLRIAITDRCNLRCRYCMPATGVPLKGHEDILRLEEIATLARVAAGTGIS
CCCHHHHHCCEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
RIRLTGGEPLVRKNVVTLVRELAAIPGLEEISLTTNGIFLGALAFSLKEAGLKRVNISLD
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEEHH
TLKKDRYRYITRRGNITSVWQGIRAALAAGLTPVKLNVVITRGFNDDEILDFARLAREEP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCC
LHIRFIELMPIGTAAASGTAYVPAEEIKGRISRVYPLEPFPDLATNGPAANFRLVGGRGS
CEEEEEEEEECCCCCCCCCEECCHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEECCCCC
VGFITPMSNHFCSRCNRLRLTADGKLRPCLYWDGEIDIKGPLRAGAPETELAAIFARAVS
EEEECCCHHHHHHHCCEEEEEECCCCCEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHC
LKPAEHHMENGWRQPRAMSQIGG
CCCHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA