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The map label for this gene is purF [H]

Identifier: 83590877

GI number: 83590877

Start: 2138044

End: 2139441

Strand: Reverse

Name: purF [H]

Synonym: Moth_2047

Alternate gene names: 83590877

Gene position: 2139441-2138044 (Counterclockwise)

Preceding gene: 83590878

Following gene: 83590876

Centisome position: 81.39

GC content: 63.45

Gene sequence:

>1398_bases
ATGTCGTCCTGGCACGAGGAGTGCGGTGTCTTTGGCATCTACGCTCCGGGCCAGGACGTGGCCCGGCTGGCCTACTACGG
ACTCTTTGCCCTCCAGCACCGCGGCCAGGAGAGCGCTGGTATCGCCGTGGCCAACGGCCGCCATATCGCCGTCCACAAGG
GTATGGGGCTGGTGGCGGAGGTCTTTAACCGGGACAACCTTCGGGCTTTACATGGTGACGTGGCCATCGGCCACGTGCGT
TACTCCACCACTGGTGCCAGTTCCCTGGTCAACGCCCAGCCCCTGGTCTTCCGCTACCTCAGGGGCATGGTGGCCATCGC
CCATAACGGTAACCTGACCAACGCCAGCGAGCTGCGGCGGGAGCTTGGAGCCAGCGGGTCTATCTTCCAGTCCTCCACCG
ATAGTGAAATCATCGTTAACCTTATCGCCCGCCACAGCCAGGAGCCCGTCGAAGCAGCCTTGCTCCATTGCCAGGAAGAG
CTTCGCGGTGCTTATTCCCTGGTGGTCATGACCGAGGAACAACTCATCGGCGTCCGTGATCCCCATGGTGTCCGGCCCCT
GTGCCTGGGCAGGATGGATGGGGCCTGGATCCTGGCCTCGGAGTCCTGCGCCCTGGATACCCTGGGGGCCGATTTTGTCC
GCGATCTGGAACCCGGGGAGATTGTCATTATCGACAGCCGGGGGGTGCGTTCCCTCCAGGGACCCCGGGCGGCCCACCGG
GCCCACTGCATTTTTGAATATGTCTACTTTGCCCGGCCGGATAGCATCCTGGACGGCGAGACCGTCAACCTGGTGCGGCG
GGAACTGGGCCGGAATCTAGCCCGGGAATACCGGGTGGCGGCCGACGCCGTCATTCCGGTACCCGACTCCGGTATTGCCG
CCGCCGCCGGCTATGCCGAGGTGGCCGGCCTGCCCTTTGTGGAGGGGTTGATGAAAAACCGCTACGTCGGCCGGACCTTC
ATCCAGCCCACCCAGGAGATGCGGGACCTGGGGGTGCGCTTGAAGCTCAACCCTATCAAGCCCATCTTAAAGGATAAAAG
GGTTATTATAATTGATGACTCCCTGGTCCGGGGAACCACCAGCCGGAGGATAGTAGCCATGCTGCGCCAGGCCGGGGTCC
GGGAGGTGCACCTGCTGGTGGCCTCGCCGCCGGTCCTGTATCCCTGTTACTACGGCATTGATACCAGCGCCCGGGGAGAG
CTCATTGCCGCCCGGTATCCCCTGGAGGACATCCGCCGCCATGTGGATGCCGACAGTCTCCACTACCTCAGCCTGGAAGG
GTTGTTTCGTTCCGTGCAGAGGGGGATGGAAGACTTCTGCGCCGCCTGCTTCACCGGCCGCTACCCCATCCCCATCCCTT
CCCCGGAGGAGGCTACCAAGTACAGCCTGGAAGGGTAG

Upstream 100 bases:

>100_bases
TGGTGGTAAGGATAAACGGCAGGACATTGTTCAATCTAAGCCTTGAGGAGATGGGGAAACAATGGCGGGAGAGCATACCG
GTGCTGATGGCCCGGTAAGG

Downstream 100 bases:

>100_bases
GGGTCAAGGTTTAACAGGCTGGCTTTTGGTTTGCAGGACTAACTTTACTGAAGAGCCGCTCATGGCGGCACGCCGCCACC
AACGAGGGCATGAAAATGCC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSSWHEECGVFGIYAPGQDVARLAYYGLFALQHRGQESAGIAVANGRHIAVHKGMGLVAEVFNRDNLRALHGDVAIGHVR
YSTTGASSLVNAQPLVFRYLRGMVAIAHNGNLTNASELRRELGASGSIFQSSTDSEIIVNLIARHSQEPVEAALLHCQEE
LRGAYSLVVMTEEQLIGVRDPHGVRPLCLGRMDGAWILASESCALDTLGADFVRDLEPGEIVIIDSRGVRSLQGPRAAHR
AHCIFEYVYFARPDSILDGETVNLVRRELGRNLAREYRVAADAVIPVPDSGIAAAAGYAEVAGLPFVEGLMKNRYVGRTF
IQPTQEMRDLGVRLKLNPIKPILKDKRVIIIDDSLVRGTTSRRIVAMLRQAGVREVHLLVASPPVLYPCYYGIDTSARGE
LIAARYPLEDIRRHVDADSLHYLSLEGLFRSVQRGMEDFCAACFTGRYPIPIPSPEEATKYSLEG

Sequences:

>Translated_465_residues
MSSWHEECGVFGIYAPGQDVARLAYYGLFALQHRGQESAGIAVANGRHIAVHKGMGLVAEVFNRDNLRALHGDVAIGHVR
YSTTGASSLVNAQPLVFRYLRGMVAIAHNGNLTNASELRRELGASGSIFQSSTDSEIIVNLIARHSQEPVEAALLHCQEE
LRGAYSLVVMTEEQLIGVRDPHGVRPLCLGRMDGAWILASESCALDTLGADFVRDLEPGEIVIIDSRGVRSLQGPRAAHR
AHCIFEYVYFARPDSILDGETVNLVRRELGRNLAREYRVAADAVIPVPDSGIAAAAGYAEVAGLPFVEGLMKNRYVGRTF
IQPTQEMRDLGVRLKLNPIKPILKDKRVIIIDDSLVRGTTSRRIVAMLRQAGVREVHLLVASPPVLYPCYYGIDTSARGE
LIAARYPLEDIRRHVDADSLHYLSLEGLFRSVQRGMEDFCAACFTGRYPIPIPSPEEATKYSLEG
>Mature_464_residues
SSWHEECGVFGIYAPGQDVARLAYYGLFALQHRGQESAGIAVANGRHIAVHKGMGLVAEVFNRDNLRALHGDVAIGHVRY
STTGASSLVNAQPLVFRYLRGMVAIAHNGNLTNASELRRELGASGSIFQSSTDSEIIVNLIARHSQEPVEAALLHCQEEL
RGAYSLVVMTEEQLIGVRDPHGVRPLCLGRMDGAWILASESCALDTLGADFVRDLEPGEIVIIDSRGVRSLQGPRAAHRA
HCIFEYVYFARPDSILDGETVNLVRRELGRNLAREYRVAADAVIPVPDSGIAAAAGYAEVAGLPFVEGLMKNRYVGRTFI
QPTQEMRDLGVRLKLNPIKPILKDKRVIIIDDSLVRGTTSRRIVAMLRQAGVREVHLLVASPPVLYPCYYGIDTSARGEL
IAARYPLEDIRRHVDADSLHYLSLEGLFRSVQRGMEDFCAACFTGRYPIPIPSPEEATKYSLEG

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=506, Percent_Identity=41.897233201581, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI1788651, Length=467, Percent_Identity=40.2569593147752, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1790167, Length=225, Percent_Identity=33.7777777777778, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=42.8870292887029, Blast_Score=351, Evalue=6e-97,
Organism=Saccharomyces cerevisiae, GI6323958, Length=471, Percent_Identity=38.6411889596603, Blast_Score=266, Evalue=7e-72,
Organism=Drosophila melanogaster, GI28573187, Length=490, Percent_Identity=44.8979591836735, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24659598, Length=486, Percent_Identity=44.238683127572, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24659604, Length=461, Percent_Identity=44.6854663774403, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51081; Mature: 50950

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSWHEECGVFGIYAPGQDVARLAYYGLFALQHRGQESAGIAVANGRHIAVHKGMGLVAE
CCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCHHHHH
VFNRDNLRALHGDVAIGHVRYSTTGASSLVNAQPLVFRYLRGMVAIAHNGNLTNASELRR
HHCCCCCEEEECCEEEEEEEEECCCHHHHCCCCHHHHHHHHHHEEEEECCCCCCHHHHHH
ELGASGSIFQSSTDSEIIVNLIARHSQEPVEAALLHCQEELRGAYSLVVMTEEQLIGVRD
HHCCCCCEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEEEHHHHCCCCC
PHGVRPLCLGRMDGAWILASESCALDTLGADFVRDLEPGEIVIIDSRGVRSLQGPRAAHR
CCCCCCEEEECCCCEEEEECCCCCHHHHCHHHHHCCCCCCEEEECCCCCCCCCCCHHHHH
AHCIFEYVYFARPDSILDGETVNLVRRELGRNLAREYRVAADAVIPVPDSGIAAAAGYAE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHCCHHH
VAGLPFVEGLMKNRYVGRTFIQPTQEMRDLGVRLKLNPIKPILKDKRVIIIDDSLVRGTT
HCCCHHHHHHHHCCCCCCHHCCCHHHHHHCCCEEEECCCCHHHCCCEEEEECCCHHCCCC
SRRIVAMLRQAGVREVHLLVASPPVLYPCYYGIDTSARGELIAARYPLEDIRRHVDADSL
HHHHHHHHHHCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEEEECCHHHHHHHCCCCCE
HYLSLEGLFRSVQRGMEDFCAACFTGRYPIPIPSPEEATKYSLEG
EEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SSWHEECGVFGIYAPGQDVARLAYYGLFALQHRGQESAGIAVANGRHIAVHKGMGLVAE
CCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCHHHHH
VFNRDNLRALHGDVAIGHVRYSTTGASSLVNAQPLVFRYLRGMVAIAHNGNLTNASELRR
HHCCCCCEEEECCEEEEEEEEECCCHHHHCCCCHHHHHHHHHHEEEEECCCCCCHHHHHH
ELGASGSIFQSSTDSEIIVNLIARHSQEPVEAALLHCQEELRGAYSLVVMTEEQLIGVRD
HHCCCCCEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEEEHHHHCCCCC
PHGVRPLCLGRMDGAWILASESCALDTLGADFVRDLEPGEIVIIDSRGVRSLQGPRAAHR
CCCCCCEEEECCCCEEEEECCCCCHHHHCHHHHHCCCCCCEEEECCCCCCCCCCCHHHHH
AHCIFEYVYFARPDSILDGETVNLVRRELGRNLAREYRVAADAVIPVPDSGIAAAAGYAE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHCCHHH
VAGLPFVEGLMKNRYVGRTFIQPTQEMRDLGVRLKLNPIKPILKDKRVIIIDDSLVRGTT
HCCCHHHHHHHHCCCCCCHHCCCHHHHHHCCCEEEECCCCHHHCCCEEEEECCCHHCCCC
SRRIVAMLRQAGVREVHLLVASPPVLYPCYYGIDTSARGELIAARYPLEDIRRHVDADSL
HHHHHHHHHHCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEEEECCHHHHHHHCCCCCE
HYLSLEGLFRSVQRGMEDFCAACFTGRYPIPIPSPEEATKYSLEG
EEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]