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The map label for this gene is hisH

Identifier: 83590863

GI number: 83590863

Start: 2122892

End: 2123524

Strand: Reverse

Name: hisH

Synonym: Moth_2033

Alternate gene names: 83590863

Gene position: 2123524-2122892 (Counterclockwise)

Preceding gene: 83590864

Following gene: 83590862

Centisome position: 80.78

GC content: 61.45

Gene sequence:

>633_bases
GTGCGGCCGATTGCTATCATCGACTATGGTATGGGCAACCTTTTAAGCGTCCAGAAGGCCCTGGACCGCCTTGGTTACCC
GGTCGAGGTAACCGACGACCCAGGAGAGATCACCGCTGCTCCCGGTGTCATTCTGCCCGGGGTGGGGGCCTTTGCCGACG
CTATGGCCAACCTGCAGCAGAAGGGGCTGGTGGCAGCCATCCGCGAGGTTGCGGAGCGGGGCGTGCCCTTGCTGGGCATC
TGCCTGGGGCTACAACTCCTTTTCTCCACCAGCGAGGAGGGGGGGCAGGTAGCCGGCCTGGATCTCCTGCCGGGAGAGGT
CAAGAGGTTACCGGCCGGCATAAAAGTTCCCCATATGGGCTGGAACCAGGTCCGTTTCCCGCGGCCGGGGGCCCTCTTCC
GGGGAATCCCCGGGGGTACAGACTTTTACTTTGTCCATTCGTATTATATTGTACCCCGGGAGGAAGAAGTGGTAACCGGC
ACCACCGAATACGGCCTGGAGTTTGCCGTCAGCATCCAGAGGGGCAACCTCTTCGGGGTCCAGTTTCACCCGGAGAAGAG
CAGCCGCCGGGGCCTGGAAGTTTTAAAGAATTTCGGGGAGTTGGTGCGCCATGCTGGTAATGCCGGCCATTGA

Upstream 100 bases:

>100_bases
CAGGCGCCGGGAGTGAACCCCGGGCCGCTTAGGCGAGGAACAGGGAGCGAAGGAAGACCAAGCCGGCTAATGAAAGAGTT
AATTCCGGAGGACTAAAGAA

Downstream 100 bases:

>100_bases
TTTGCGGGAGGGGCGATGCGTGCGCCTCTACCAGGGACGTATCGAAGCCGAGACCGTCTATTCCACGGATCCGGTGGCCG
TTGCCCGGGGCTGGGTGGAA

Product: imidazole glycerol phosphate synthase subunit hisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MRPIAIIDYGMGNLLSVQKALDRLGYPVEVTDDPGEITAAPGVILPGVGAFADAMANLQQKGLVAAIREVAERGVPLLGI
CLGLQLLFSTSEEGGQVAGLDLLPGEVKRLPAGIKVPHMGWNQVRFPRPGALFRGIPGGTDFYFVHSYYIVPREEEVVTG
TTEYGLEFAVSIQRGNLFGVQFHPEKSSRRGLEVLKNFGELVRHAGNAGH

Sequences:

>Translated_210_residues
MRPIAIIDYGMGNLLSVQKALDRLGYPVEVTDDPGEITAAPGVILPGVGAFADAMANLQQKGLVAAIREVAERGVPLLGI
CLGLQLLFSTSEEGGQVAGLDLLPGEVKRLPAGIKVPHMGWNQVRFPRPGALFRGIPGGTDFYFVHSYYIVPREEEVVTG
TTEYGLEFAVSIQRGNLFGVQFHPEKSSRRGLEVLKNFGELVRHAGNAGH
>Mature_210_residues
MRPIAIIDYGMGNLLSVQKALDRLGYPVEVTDDPGEITAAPGVILPGVGAFADAMANLQQKGLVAAIREVAERGVPLLGI
CLGLQLLFSTSEEGGQVAGLDLLPGEVKRLPAGIKVPHMGWNQVRFPRPGALFRGIPGGTDFYFVHSYYIVPREEEVVTG
TTEYGLEFAVSIQRGNLFGVQFHPEKSSRRGLEVLKNFGELVRHAGNAGH

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=41, Blast_Score=140, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6319725, Length=210, Percent_Identity=36.1904761904762, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_MOOTA (Q2RGW0)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430872.1
- ProteinModelPortal:   Q2RGW0
- SMR:   Q2RGW0
- STRING:   Q2RGW0
- GeneID:   3831408
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2033
- NMPDR:   fig|264732.9.peg.2054
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- PhylomeDB:   Q2RGW0
- BioCyc:   MTHE264732:MOTH_2033-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.- [C]

Molecular weight: Translated: 22590; Mature: 22590

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 183-183 ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPIAIIDYGMGNLLSVQKALDRLGYPVEVTDDPGEITAAPGVILPGVGAFADAMANLQQ
CCCEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCEEECCCEEECCCHHHHHHHHHHHH
KGLVAAIREVAERGVPLLGICLGLQLLFSTSEEGGQVAGLDLLPGEVKRLPAGIKVPHMG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCCCCCCC
WNQVRFPRPGALFRGIPGGTDFYFVHSYYIVPREEEVVTGTTEYGLEFAVSIQRGNLFGV
CCCCCCCCCCHHHCCCCCCCCEEEEEEEEEEECCCCEEECCHHHCEEEEEEEECCCEEEE
QFHPEKSSRRGLEVLKNFGELVRHAGNAGH
EECCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRPIAIIDYGMGNLLSVQKALDRLGYPVEVTDDPGEITAAPGVILPGVGAFADAMANLQQ
CCCEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCEEECCCEEECCCHHHHHHHHHHHH
KGLVAAIREVAERGVPLLGICLGLQLLFSTSEEGGQVAGLDLLPGEVKRLPAGIKVPHMG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCCCCCCC
WNQVRFPRPGALFRGIPGGTDFYFVHSYYIVPREEEVVTGTTEYGLEFAVSIQRGNLFGV
CCCCCCCCCCHHHCCCCCCCCEEEEEEEEEEECCCCEEECCHHHCEEEEEEEECCCEEEE
QFHPEKSSRRGLEVLKNFGELVRHAGNAGH
EECCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA