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The map label for this gene is hisF

Identifier: 83590861

GI number: 83590861

Start: 2121372

End: 2122130

Strand: Reverse

Name: hisF

Synonym: Moth_2031

Alternate gene names: 83590861

Gene position: 2122130-2121372 (Counterclockwise)

Preceding gene: 83590862

Following gene: 83590860

Centisome position: 80.73

GC content: 65.09

Gene sequence:

>759_bases
TTGTTGACCAAAAGAATTATTCCCTGCCTGGATGTTGACCACGGTCGGGTGGTCAAGGGTACTAACTTTTTAAATTTGAG
GGACGCCGGCGACCCGGTGGAGCTGGCGGCCGCCTACGACCGGGAGGGCGCCGACGAGCTGGTATTCTTGGATATTTCAG
CCTCGGTGGAGGGCCGGGATATTATGATCGACGTGGCCCGGCGCACGGCGGCCCAGGTCTTTATCCCCTTCACCGTCGGC
GGCGGCTTGCGCTCCCTGGAGGACATCCGGGCCATGCTGGCCGCCGGGGCGGACAAAATTTCCCTGAATACGGCCGCCGT
CCTGGACCCGGACCTGGTGGCCACGGCGGCCCGACGTTTCGGCAGCCAGTGCGTGGTGGTGGCCATCGACGCCCGGCGTA
CCGGCCCGGGTCGCTGGGAGGTCTATACCCACGGCGGCCGGAGGCCGACCGGCAGGGATGCCGTGGCCTGGGCCCGGCAG
GTGGAGGAACTGGGGGCCGGGGAGATCCTGCTGACCAGCATGGACTGCGACGGTACCCTGGACGGCTACGACCTGGAACT
GACGGCCACCGTCAGCCAGGCCGTGAATATCCCGGTTATCGCCTCCGGCGGGGTGGGCAAGCTGGAACACCTTTATGAGG
GTTTGACGGCAGGAAGGGCCGACGCTGTCCTGGCGGCTTCTATTTTCCATTTTGGCACCTATACCATCAAAGAGGCCAAG
GACTACCTGGCGGCCCGCGGCGTCCCGGTGCGGTTATAG

Upstream 100 bases:

>100_bases
AATTTCACCCTGGAAGAGGCCCTGGCCGTAGCCGCAGATGGAGATTAAAGGGCGGGGTGAATTCGCCTTTTGTAGAGCCA
GCGGAAAGAGGCGGTATAGG

Downstream 100 bases:

>100_bases
GAGGTAAACCTCATGAGTGTCACCATCCCTGAGGATATTCACTTTAACGCCGAGGGCCTGATCCCGGCCATCATCCAGGA
CGCGGCCAGCGGCCAGGTCT

Product: imidazole glycerol phosphate synthase subunit hisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLTKRIIPCLDVDHGRVVKGTNFLNLRDAGDPVELAAAYDREGADELVFLDISASVEGRDIMIDVARRTAAQVFIPFTVG
GGLRSLEDIRAMLAAGADKISLNTAAVLDPDLVATAARRFGSQCVVVAIDARRTGPGRWEVYTHGGRRPTGRDAVAWARQ
VEELGAGEILLTSMDCDGTLDGYDLELTATVSQAVNIPVIASGGVGKLEHLYEGLTAGRADAVLAASIFHFGTYTIKEAK
DYLAARGVPVRL

Sequences:

>Translated_252_residues
MLTKRIIPCLDVDHGRVVKGTNFLNLRDAGDPVELAAAYDREGADELVFLDISASVEGRDIMIDVARRTAAQVFIPFTVG
GGLRSLEDIRAMLAAGADKISLNTAAVLDPDLVATAARRFGSQCVVVAIDARRTGPGRWEVYTHGGRRPTGRDAVAWARQ
VEELGAGEILLTSMDCDGTLDGYDLELTATVSQAVNIPVIASGGVGKLEHLYEGLTAGRADAVLAASIFHFGTYTIKEAK
DYLAARGVPVRL
>Mature_252_residues
MLTKRIIPCLDVDHGRVVKGTNFLNLRDAGDPVELAAAYDREGADELVFLDISASVEGRDIMIDVARRTAAQVFIPFTVG
GGLRSLEDIRAMLAAGADKISLNTAAVLDPDLVATAARRFGSQCVVVAIDARRTGPGRWEVYTHGGRRPTGRDAVAWARQ
VEELGAGEILLTSMDCDGTLDGYDLELTATVSQAVNIPVIASGGVGKLEHLYEGLTAGRADAVLAASIFHFGTYTIKEAK
DYLAARGVPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=46.692607003891, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI87082028, Length=238, Percent_Identity=28.9915966386555, Blast_Score=89, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=319, Percent_Identity=35.7366771159875, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_MOOTA (Q2RGW2)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430870.1
- ProteinModelPortal:   Q2RGW2
- SMR:   Q2RGW2
- STRING:   Q2RGW2
- GeneID:   3831406
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2031
- NMPDR:   fig|264732.9.peg.2052
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q2RGW2
- BioCyc:   MTHE264732:MOTH_2031-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26838; Mature: 26838

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDVDHGRVVKGTNFLNLRDAGDPVELAAAYDREGADELVFLDISASVEGRD
CCCCCCCCEEECCCCEEEECCCEEEECCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCE
IMIDVARRTAAQVFIPFTVGGGLRSLEDIRAMLAAGADKISLNTAAVLDPDLVATAARRF
EEEEEHHCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCEEECCEEEEECCHHHHHHHHHC
GSQCVVVAIDARRTGPGRWEVYTHGGRRPTGRDAVAWARQVEELGAGEILLTSMDCDGTL
CCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCC
DGYDLELTATVSQAVNIPVIASGGVGKLEHLYEGLTAGRADAVLAASIFHFGTYTIKEAK
CCCEEEEEEEHHHHCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEHHHHH
DYLAARGVPVRL
HHHHHCCCEECC
>Mature Secondary Structure
MLTKRIIPCLDVDHGRVVKGTNFLNLRDAGDPVELAAAYDREGADELVFLDISASVEGRD
CCCCCCCCEEECCCCEEEECCCEEEECCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCE
IMIDVARRTAAQVFIPFTVGGGLRSLEDIRAMLAAGADKISLNTAAVLDPDLVATAARRF
EEEEEHHCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCEEECCEEEEECCHHHHHHHHHC
GSQCVVVAIDARRTGPGRWEVYTHGGRRPTGRDAVAWARQVEELGAGEILLTSMDCDGTL
CCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCC
DGYDLELTATVSQAVNIPVIASGGVGKLEHLYEGLTAGRADAVLAASIFHFGTYTIKEAK
CCCEEEEEEEHHHHCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEHHHHH
DYLAARGVPVRL
HHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA