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The map label for this gene is gcvH [H]

Identifier: 83590776

GI number: 83590776

Start: 2018506

End: 2018898

Strand: Reverse

Name: gcvH [H]

Synonym: Moth_1944

Alternate gene names: 83590776

Gene position: 2018898-2018506 (Counterclockwise)

Preceding gene: 83590777

Following gene: 83590775

Centisome position: 76.8

GC content: 55.98

Gene sequence:

>393_bases
ATGAATTTCCCGGCTCATCTGCATTATAGTAAAGACCATGAGTGGGTAGAAGTCGATGGTAACCGTGCCCGTATCGGTAT
TACCGACTATGCCCAGGAGTCCCTGGGGGACATAGTTTTTGTGGAACTGCCCCAGGTAGGGGATGAACTGGCGACGGGCG
ACAGCTTTGGGGTCGTCGAGTCGGTAAAGTCGGCTTCCGACGTCTATGCGCCGGTGGGCGGCAAGGTGGTGGCTGTTAAC
GAAGCCCTCCTGGACGCCCCCCAGGACATCAACGCCGACCCCTACGGCAAGGGCTGGATGATTGAACTGGAATTGAGCGA
TCCGAGTGAAGTAGAAAGCCTCATGGATGCCAGTGCTTACCTGGAACTGGTAAAGGAGGAGAAGGGGGAGTAA

Upstream 100 bases:

>100_bases
CATCCGCGGCCGTGTCAACCGCGCCCGGGTGGTGAAACTCCCCTTCTATCGCCGCCCCAAAAAGTAAGCCGAAATCTTTT
TAAAAGGAGGAATTAATGGC

Downstream 100 bases:

>100_bases
GGGGATGACCTACATTCCCACCACGGCGGCGGAACAGCAGCAGATGCTGGCGGCCTGCGGTGCCCACCGGATGGAAGAGC
TTTTTAGTGATGTACCTGCC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MNFPAHLHYSKDHEWVEVDGNRARIGITDYAQESLGDIVFVELPQVGDELATGDSFGVVESVKSASDVYAPVGGKVVAVN
EALLDAPQDINADPYGKGWMIELELSDPSEVESLMDASAYLELVKEEKGE

Sequences:

>Translated_130_residues
MNFPAHLHYSKDHEWVEVDGNRARIGITDYAQESLGDIVFVELPQVGDELATGDSFGVVESVKSASDVYAPVGGKVVAVN
EALLDAPQDINADPYGKGWMIELELSDPSEVESLMDASAYLELVKEEKGE
>Mature_130_residues
MNFPAHLHYSKDHEWVEVDGNRARIGITDYAQESLGDIVFVELPQVGDELATGDSFGVVESVKSASDVYAPVGGKVVAVN
EALLDAPQDINADPYGKGWMIELELSDPSEVESLMDASAYLELVKEEKGE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=43.2203389830509, Blast_Score=117, Evalue=2e-27,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=42.3728813559322, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1789271, Length=128, Percent_Identity=54.6875, Blast_Score=143, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=46.7213114754098, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=119, Percent_Identity=46.218487394958, Blast_Score=116, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=42.9752066115703, Blast_Score=106, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=48.2456140350877, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14127; Mature: 14127

Theoretical pI: Translated: 3.87; Mature: 3.87

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPAHLHYSKDHEWVEVDGNRARIGITDYAQESLGDIVFVELPQVGDELATGDSFGVVE
CCCCCCEEECCCCCEEEECCCEEEEEEHHHHHHHHCCEEEEECCCCCCHHCCCCCCHHHH
SVKSASDVYAPVGGKVVAVNEALLDAPQDINADPYGKGWMIELELSDPSEVESLMDASAY
HHHHHHHEECCCCCEEEEECHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
LELVKEEKGE
HHHHHHHCCC
>Mature Secondary Structure
MNFPAHLHYSKDHEWVEVDGNRARIGITDYAQESLGDIVFVELPQVGDELATGDSFGVVE
CCCCCCEEECCCCCEEEECCCEEEEEEHHHHHHHHCCEEEEECCCCCCHHCCCCCCHHHH
SVKSASDVYAPVGGKVVAVNEALLDAPQDINADPYGKGWMIELELSDPSEVESLMDASAY
HHHHHHHEECCCCCEEEEECHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
LELVKEEKGE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA