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The map label for this gene is cca [H]

Identifier: 83590210

GI number: 83590210

Start: 1406008

End: 1407159

Strand: Reverse

Name: cca [H]

Synonym: Moth_1363

Alternate gene names: 83590210

Gene position: 1407159-1406008 (Counterclockwise)

Preceding gene: 83590211

Following gene: 83590209

Centisome position: 53.53

GC content: 64.15

Gene sequence:

>1152_bases
ATGATTCCCTACCGGAAGCTTTTTAACCAGCACCAGCTAGCCATCCTGGCCCGGGCCAGGAAAATAGCCCACCTCAAGGG
CCAGCGAGTTTACCTGGTAGGGGGTACGACCCGGGATCTCTTGCTGGGACGGCCGGATGTCGACCTGGATCTGGTGGTCG
AGGGGAACGGCCTCCTCCTGGCCCGGGAACTGGCCCGGCAACTGGGAGGGCGCCTGGTCTACCACGAGCGCTTCCTGACG
GCTACCATTTACTGGGCCGGGGAGCGGGTGGATGTGGTCACCGCCAGGAAGGAGTATTATCCCGAGCCGGGAGCGCTGCC
GGAGGTCGAGGCCGCCACCCTGGCCGAGGATCTGGCCCGGCGCGATTTTAGCATTAACGCCATGGCCCTGCCATTAACGG
CCCGGGACCTGTGGGAGATCCGGGATCCCTTTAACGGCCGGGCCGACCTGGCTGCCGGTAAAATAAGGGTCCTCCACCAG
GCGAGTTTCCGCGACGATCCCACCCGTATCCTGCGGGGGGTACGCCTGGGAACCAGGTTACATTTCCAGCTGGCCAGTGA
GACCAGGGAACTAGCCCGGGAAGCCCTGGCTGCCGGCTACCTGGAACTGGTATCACCCCGGCGTTTCTGGCAGGAGTTTA
TCCTGCTCCTCAAGGAATCGCGGCCGGTTGCTGCCTGGGAAGCCCTGCTGGCCCTGGGATGGCGGGGCCTGCCGGGGAGC
GGTCCCCCGGATGTAGCTGCCGGTTACCGGGCGGAGAAACTCCTGCGCCAGTGGCAGTGGCTGGGCCAGCACCCCCCTGA
CGCCAGCCTGGTGTATTTTTTAACGGCCACGGCTAATTTGAGCCTGGCAGCCCTGGAGGAGGTTTTGGATCACCTGAACT
GGGGCCGCAGGCGTCGTCGGGTAATTAACGCTGCCAGGGTTTTACAGCAAGGGGGCTGTTTTGGACCTGAACCGGGTTTG
CCCCCGGGAGAAATGATGACCGGGTCGCGCCCGGCCCCGGAGGCGCTGATCTTTGCCCTGGCCCAGGCCGGGTGGAGCCA
GTTGCCGGGCTGGGCCCTACCTGGTGGGAAAGGAGTCGGCCATGCACCTGCAGAGCTTGTCCCAGTTAATCCTGGGTATT
CCAGCCATCCTGATGGCCCTGACCTTTCATGA

Upstream 100 bases:

>100_bases
CGTCGCCGACTTGATTATAAAGCGGGAGGATTACAGCGACCTGATCCGTAACGACGTGATCCCGGCCCGCCTGGCCTGCC
CTCGCTAGGCCGGTACTGTC

Downstream 100 bases:

>100_bases
GTATGCCCACGGGAAGGCAGCCTACTTTCTGGGAGATCCCACGGCCAGAAATCAGGGGCGGTTAACCTTAAATCCCCTGG
CCCACCTGGATCCCCTGGGA

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MIPYRKLFNQHQLAILARARKIAHLKGQRVYLVGGTTRDLLLGRPDVDLDLVVEGNGLLLARELARQLGGRLVYHERFLT
ATIYWAGERVDVVTARKEYYPEPGALPEVEAATLAEDLARRDFSINAMALPLTARDLWEIRDPFNGRADLAAGKIRVLHQ
ASFRDDPTRILRGVRLGTRLHFQLASETRELAREALAAGYLELVSPRRFWQEFILLLKESRPVAAWEALLALGWRGLPGS
GPPDVAAGYRAEKLLRQWQWLGQHPPDASLVYFLTATANLSLAALEEVLDHLNWGRRRRRVINAARVLQQGGCFGPEPGL
PPGEMMTGSRPAPEALIFALAQAGWSQLPGWALPGGKGVGHAPAELVPVNPGYSSHPDGPDLS

Sequences:

>Translated_383_residues
MIPYRKLFNQHQLAILARARKIAHLKGQRVYLVGGTTRDLLLGRPDVDLDLVVEGNGLLLARELARQLGGRLVYHERFLT
ATIYWAGERVDVVTARKEYYPEPGALPEVEAATLAEDLARRDFSINAMALPLTARDLWEIRDPFNGRADLAAGKIRVLHQ
ASFRDDPTRILRGVRLGTRLHFQLASETRELAREALAAGYLELVSPRRFWQEFILLLKESRPVAAWEALLALGWRGLPGS
GPPDVAAGYRAEKLLRQWQWLGQHPPDASLVYFLTATANLSLAALEEVLDHLNWGRRRRRVINAARVLQQGGCFGPEPGL
PPGEMMTGSRPAPEALIFALAQAGWSQLPGWALPGGKGVGHAPAELVPVNPGYSSHPDGPDLS
>Mature_383_residues
MIPYRKLFNQHQLAILARARKIAHLKGQRVYLVGGTTRDLLLGRPDVDLDLVVEGNGLLLARELARQLGGRLVYHERFLT
ATIYWAGERVDVVTARKEYYPEPGALPEVEAATLAEDLARRDFSINAMALPLTARDLWEIRDPFNGRADLAAGKIRVLHQ
ASFRDDPTRILRGVRLGTRLHFQLASETRELAREALAAGYLELVSPRRFWQEFILLLKESRPVAAWEALLALGWRGLPGS
GPPDVAAGYRAEKLLRQWQWLGQHPPDASLVYFLTATANLSLAALEEVLDHLNWGRRRRRVINAARVLQQGGCFGPEPGL
PPGEMMTGSRPAPEALIFALAQAGWSQLPGWALPGGKGVGHAPAELVPVNPGYSSHPDGPDLS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=216, Percent_Identity=35.6481481481481, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081691, Length=231, Percent_Identity=29.4372294372294, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 42336; Mature: 42336

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPYRKLFNQHQLAILARARKIAHLKGQRVYLVGGTTRDLLLGRPDVDLDLVVEGNGLLL
CCCHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHEECCCCCCEEEEECCCCEEH
ARELARQLGGRLVYHERFLTATIYWAGERVDVVTARKEYYPEPGALPEVEAATLAEDLAR
HHHHHHHHCCEEEEEHHEEEEEEEECCCEEEEEEEHHHHCCCCCCCCCCHHHHHHHHHHH
RDFSINAMALPLTARDLWEIRDPFNGRADLAAGKIRVLHQASFRDDPTRILRGVRLGTRL
CCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEE
HFQLASETRELAREALAAGYLELVSPRRFWQEFILLLKESRPVAAWEALLALGWRGLPGS
EEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
GPPDVAAGYRAEKLLRQWQWLGQHPPDASLVYFLTATANLSLAALEEVLDHLNWGRRRRR
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
VINAARVLQQGGCFGPEPGLPPGEMMTGSRPAPEALIFALAQAGWSQLPGWALPGGKGVG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HAPAELVPVNPGYSSHPDGPDLS
CCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MIPYRKLFNQHQLAILARARKIAHLKGQRVYLVGGTTRDLLLGRPDVDLDLVVEGNGLLL
CCCHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHEECCCCCCEEEEECCCCEEH
ARELARQLGGRLVYHERFLTATIYWAGERVDVVTARKEYYPEPGALPEVEAATLAEDLAR
HHHHHHHHCCEEEEEHHEEEEEEEECCCEEEEEEEHHHHCCCCCCCCCCHHHHHHHHHHH
RDFSINAMALPLTARDLWEIRDPFNGRADLAAGKIRVLHQASFRDDPTRILRGVRLGTRL
CCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEE
HFQLASETRELAREALAAGYLELVSPRRFWQEFILLLKESRPVAAWEALLALGWRGLPGS
EEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
GPPDVAAGYRAEKLLRQWQWLGQHPPDASLVYFLTATANLSLAALEEVLDHLNWGRRRRR
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
VINAARVLQQGGCFGPEPGLPPGEMMTGSRPAPEALIFALAQAGWSQLPGWALPGGKGVG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HAPAELVPVNPGYSSHPDGPDLS
CCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA