Click here to switch to the map view.

The map label for this gene is cysG [H]

Identifier: 83590099

GI number: 83590099

Start: 1293057

End: 1293701

Strand: Reverse

Name: cysG [H]

Synonym: Moth_1251

Alternate gene names: 83590099

Gene position: 1293701-1293057 (Counterclockwise)

Preceding gene: 83590100

Following gene: 83590098

Centisome position: 49.21

GC content: 58.29

Gene sequence:

>645_bases
ATGGGCTTTTACCCACTGGTTATTGACCTGACTGACCGCCCCTGCCTGGTGATTGGAGGCGGTACTGTTAGTGAACGTAA
GGTTTTGAATTTACTGGAAGCCGGGGCCAGAATCACCCTGGTCAGCCCTGTAGTTACCCGTACCCTCGCCGACCTGGCCT
TGGCCAGCCAGGTAGTATGGTGGCGGCGGGAGTACCGGGAGGGCGACCTGAAGGGTATGACCATGGTTTTTGCTGCTACC
AATGATCCCGAGCTTAACGCGCGGATAGCCGTAGCCTGCCACCAGGCCGGCATCCTGGTTAATGTGGTCGATAACCAGGA
GTTATGCACCTTCAACGTCCCGGCGGTTGTCAGGCGGGGCGATTTACAGATAGCCATCTCCACCGCCGGCAAGAGCCCGG
CCCTGGCCCGCCAGTTACGTAAGCAGCTAGAAGCCGAAATTGGCCCGGAGTACGGGCCCTGGGTAGATTTTTTAGGTGAA
ATACGCCCCCTGCTCAAAAGCGTCTGGCCCAACGATCAAAAAAGGCGGGAGGAACTCCTGCGCCGCCTGGCCGGTGATGA
AATCCTATTCCGCCTGGTGGCCCGGGGTAAAGAAGAACTGGCAAAGGAGCGGGTCAAGCAGTGTTTGTCGTTGCCGCCGG
TCTAA

Upstream 100 bases:

>100_bases
ATTTGGAAAGGGCCAGAAAGCAGGCATCCTACCTGCCTCCGGGTGTGGCCAGGGATACCCTCACAAGCCTGACGTATTAT
ATAAGGACGCGTGGTTTTTA

Downstream 100 bases:

>100_bases
ATCATCGCACGGCGCCGGTAGCTATCCGGGAGCAGCTGGCCTTTGCTCGTCACGGCCTGCCGCCTGCTTTATGCCGTCTA
AAGGAAGAGGCTGGCGTTGA

Product: precorrin-2 dehydrogenase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MGFYPLVIDLTDRPCLVIGGGTVSERKVLNLLEAGARITLVSPVVTRTLADLALASQVVWWRREYREGDLKGMTMVFAAT
NDPELNARIAVACHQAGILVNVVDNQELCTFNVPAVVRRGDLQIAISTAGKSPALARQLRKQLEAEIGPEYGPWVDFLGE
IRPLLKSVWPNDQKRREELLRRLAGDEILFRLVARGKEELAKERVKQCLSLPPV

Sequences:

>Translated_214_residues
MGFYPLVIDLTDRPCLVIGGGTVSERKVLNLLEAGARITLVSPVVTRTLADLALASQVVWWRREYREGDLKGMTMVFAAT
NDPELNARIAVACHQAGILVNVVDNQELCTFNVPAVVRRGDLQIAISTAGKSPALARQLRKQLEAEIGPEYGPWVDFLGE
IRPLLKSVWPNDQKRREELLRRLAGDEILFRLVARGKEELAKERVKQCLSLPPV
>Mature_213_residues
GFYPLVIDLTDRPCLVIGGGTVSERKVLNLLEAGARITLVSPVVTRTLADLALASQVVWWRREYREGDLKGMTMVFAATN
DPELNARIAVACHQAGILVNVVDNQELCTFNVPAVVRRGDLQIAISTAGKSPALARQLRKQLEAEIGPEYGPWVDFLGEI
RPLLKSVWPNDQKRREELLRRLAGDEILFRLVARGKEELAKERVKQCLSLPPV

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG1648

COG function: function code H; Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=212, Percent_Identity=31.1320754716981, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 23806; Mature: 23675

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFYPLVIDLTDRPCLVIGGGTVSERKVLNLLEAGARITLVSPVVTRTLADLALASQVVW
CCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHH
WRREYREGDLKGMTMVFAATNDPELNARIAVACHQAGILVNVVDNQELCTFNVPAVVRRG
HHHHHCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCEEEEEECCCCEEEECCCHHEECC
DLQIAISTAGKSPALARQLRKQLEAEIGPEYGPWVDFLGEIRPLLKSVWPNDQKRREELL
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RRLAGDEILFRLVARGKEELAKERVKQCLSLPPV
HHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GFYPLVIDLTDRPCLVIGGGTVSERKVLNLLEAGARITLVSPVVTRTLADLALASQVVW
CCEEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHH
WRREYREGDLKGMTMVFAATNDPELNARIAVACHQAGILVNVVDNQELCTFNVPAVVRRG
HHHHHCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCEEEEEECCCCEEEECCCHHEECC
DLQIAISTAGKSPALARQLRKQLEAEIGPEYGPWVDFLGEIRPLLKSVWPNDQKRREELL
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RRLAGDEILFRLVARGKEELAKERVKQCLSLPPV
HHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]