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The map label for this gene is hemD [H]

Identifier: 83590096

GI number: 83590096

Start: 1289275

End: 1290837

Strand: Reverse

Name: hemD [H]

Synonym: Moth_1248

Alternate gene names: 83590096

Gene position: 1290837-1289275 (Counterclockwise)

Preceding gene: 83590097

Following gene: 83590095

Centisome position: 49.1

GC content: 62.83

Gene sequence:

>1563_bases
GTGGAAAATACCAGGGGTAAAGTTTTTTTAGTTGGTGCCGGCCCCGGCGACCCGGGCCTGTTGACTGTCAAAGGACGGGA
GTGCCTGGCCCGGGCCGGGGCTGTAGTCTACGATCGCTTGCTCAACCCTGCTTTACTGGAATATGCCCCGCCGGAGGCTG
TTAAGATCTACGTCGGTAAAGCGCCGGATCGCCATGCCTTAAGCCAGGACGAGATCAACGACCTCCTGGTGGACCTGGCG
CGGCAGGGGAAACAGGTGGTGCGCCTTAAAGGGGGCGACCCCTTTGTCTTTGGCCGCGGCGGGGAAGAAGCCCTGGCCCT
ACGAGCTGCCGGCATCCCCTTCGAAGTAGTGCCAGGGGTCACGGCCGCCGTGGCCGTGCCGGCCTATGCCGGTATTCCGG
TAACCCACCGGGGCCTGGCTTCAACGGCGGCCTTCATAACTGGCAACGAAGACCCCCGGAAAGGAAACAGCGCCATTAAC
TGGGAGGGTCTGGCCCGGGCCGTTGACACCCTGGTTTTTTTAATGGGTATGGCCAACCTGCCCTACATTGTCAGCCGCCT
CCTGGCCTGCGGTCGCTCTCCCGATACACCGGTGGCTCTCATCCGCTGGGGCACCAGGGCCGAGCAGGAGACGCTGACCG
GCACCCTGGCGGATATCGAGGGCCGGGCCCAGGAAGCCGGCTTCCGCAACCCGGCCATTATCATCATCGGCCAGGTGGTC
AACCTGCGTTCAACCCTGGCCTGGCTGGAGGATAAACCCCTCTTTGGCCGGCGGGTGATCGTTACCCGCCCACGGGCCCA
GGCAGAAGGCTTGACCCGAAGCCTGGCGGACCTGGGGGCGGAAGTCATAAATTTCCCGGTGATTAGGACGGAACCTCCGG
CCGACTGGCACCCCTTGGATACCGCCCTGGACGCTATCGGGGAGTTTGATTGGATCATATTTACCAGTGCCAACGGTGTC
CGTTACTTCTGGCGACGACTTCTGGAACGACACCAGGATATCCGTTCCCTGGCCGGAATCAGGATTGCGGCCATAGGGCC
GGCCACTTCCCGTGCCCTGAAGGAACGGGGCCTCCTGACCGATTGGCAACCCCGGGAATATGTAGCCGAAGCCGTGGCTT
CCGGACTGGGACCCCGGGTCAGGGGCCGGCGGGTTCTCCTACCCCGGGCTGATATCGCCCGGCCCTTCCTGGCCGTGGAC
CTCCGCCGCCAGGGGGCAGAAGTAACGGAGGTAACGGCCTACCGGACGGTAAAGAATGAAGAAAACGCCGGGTCCCTTAA
AGAAATGCTGGCCGCCGGTAAAGTAGCCGCCGTCACCTTTACCAGTTCTTCGACGGTACGGGCCTTCCTTGACCTGCTCG
GGGACGGGGCCCTGGATTTAGTGCAAGGAATAGACGTTTTTTGCCTCGGCCCGGTCACGGCGGCCACAGCCCGGGAGGCC
GGCCTGCAGGTAGCCGCCACGGCCGGCGAGTATACAGAAGAGGGGCTGGTGCGGGCCATGGAAAACTATTATACAACCAT
AAGGGCCGGGGGCGGAGATAGCAACCAATCCCGGAGCCTTTAA

Upstream 100 bases:

>100_bases
TCCGGGAGTACCCGGGACCCGGAGGCTGCCGGCCGGGAACTGGCCCGGAAACTCCTCGCCCGGGGTGCGGGTGAGATTTT
GCAGGAGGTTAAGGCCCAAA

Downstream 100 bases:

>100_bases
GAGTAACCGCAAGGCGGGTGAGGAAAACTGATCAGTCTCACAAAGCTGCTTTTTGATGATAACTACTTTGGCGACAACCT
GCGTTACAGCCAGGCTGTCC

Product: uroporphyrinogen-III C-methyltransferase / uroporphyrinogen-III synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MENTRGKVFLVGAGPGDPGLLTVKGRECLARAGAVVYDRLLNPALLEYAPPEAVKIYVGKAPDRHALSQDEINDLLVDLA
RQGKQVVRLKGGDPFVFGRGGEEALALRAAGIPFEVVPGVTAAVAVPAYAGIPVTHRGLASTAAFITGNEDPRKGNSAIN
WEGLARAVDTLVFLMGMANLPYIVSRLLACGRSPDTPVALIRWGTRAEQETLTGTLADIEGRAQEAGFRNPAIIIIGQVV
NLRSTLAWLEDKPLFGRRVIVTRPRAQAEGLTRSLADLGAEVINFPVIRTEPPADWHPLDTALDAIGEFDWIIFTSANGV
RYFWRRLLERHQDIRSLAGIRIAAIGPATSRALKERGLLTDWQPREYVAEAVASGLGPRVRGRRVLLPRADIARPFLAVD
LRRQGAEVTEVTAYRTVKNEENAGSLKEMLAAGKVAAVTFTSSSTVRAFLDLLGDGALDLVQGIDVFCLGPVTAATAREA
GLQVAATAGEYTEEGLVRAMENYYTTIRAGGGDSNQSRSL

Sequences:

>Translated_520_residues
MENTRGKVFLVGAGPGDPGLLTVKGRECLARAGAVVYDRLLNPALLEYAPPEAVKIYVGKAPDRHALSQDEINDLLVDLA
RQGKQVVRLKGGDPFVFGRGGEEALALRAAGIPFEVVPGVTAAVAVPAYAGIPVTHRGLASTAAFITGNEDPRKGNSAIN
WEGLARAVDTLVFLMGMANLPYIVSRLLACGRSPDTPVALIRWGTRAEQETLTGTLADIEGRAQEAGFRNPAIIIIGQVV
NLRSTLAWLEDKPLFGRRVIVTRPRAQAEGLTRSLADLGAEVINFPVIRTEPPADWHPLDTALDAIGEFDWIIFTSANGV
RYFWRRLLERHQDIRSLAGIRIAAIGPATSRALKERGLLTDWQPREYVAEAVASGLGPRVRGRRVLLPRADIARPFLAVD
LRRQGAEVTEVTAYRTVKNEENAGSLKEMLAAGKVAAVTFTSSSTVRAFLDLLGDGALDLVQGIDVFCLGPVTAATAREA
GLQVAATAGEYTEEGLVRAMENYYTTIRAGGGDSNQSRSL
>Mature_520_residues
MENTRGKVFLVGAGPGDPGLLTVKGRECLARAGAVVYDRLLNPALLEYAPPEAVKIYVGKAPDRHALSQDEINDLLVDLA
RQGKQVVRLKGGDPFVFGRGGEEALALRAAGIPFEVVPGVTAAVAVPAYAGIPVTHRGLASTAAFITGNEDPRKGNSAIN
WEGLARAVDTLVFLMGMANLPYIVSRLLACGRSPDTPVALIRWGTRAEQETLTGTLADIEGRAQEAGFRNPAIIIIGQVV
NLRSTLAWLEDKPLFGRRVIVTRPRAQAEGLTRSLADLGAEVINFPVIRTEPPADWHPLDTALDAIGEFDWIIFTSANGV
RYFWRRLLERHQDIRSLAGIRIAAIGPATSRALKERGLLTDWQPREYVAEAVASGLGPRVRGRRVLLPRADIARPFLAVD
LRRQGAEVTEVTAYRTVKNEENAGSLKEMLAAGKVAAVTFTSSSTVRAFLDLLGDGALDLVQGIDVFCLGPVTAATAREA
GLQVAATAGEYTEEGLVRAMENYYTTIRAGGGDSNQSRSL

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=250, Percent_Identity=46, Blast_Score=216, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6322922, Length=236, Percent_Identity=32.2033898305085, Blast_Score=117, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 55828; Mature: 55828

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTRGKVFLVGAGPGDPGLLTVKGRECLARAGAVVYDRLLNPALLEYAPPEAVKIYVGK
CCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECC
APDRHALSQDEINDLLVDLARQGKQVVRLKGGDPFVFGRGGEEALALRAAGIPFEVVPGV
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCEEEEEECCCCEEECCCC
TAAVAVPAYAGIPVTHRGLASTAAFITGNEDPRKGNSAINWEGLARAVDTLVFLMGMANL
HHHHHHHHHCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PYIVSRLLACGRSPDTPVALIRWGTRAEQETLTGTLADIEGRAQEAGFRNPAIIIIGQVV
HHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHCCCCCCEEEEEECHH
NLRSTLAWLEDKPLFGRRVIVTRPRAQAEGLTRSLADLGAEVINFPVIRTEPPADWHPLD
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHH
TALDAIGEFDWIIFTSANGVRYFWRRLLERHQDIRSLAGIRIAAIGPATSRALKERGLLT
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHCCCCC
DWQPREYVAEAVASGLGPRVRGRRVLLPRADIARPFLAVDLRRQGAEVTEVTAYRTVKNE
CCCHHHHHHHHHHHCCCCCCCCCEEECCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCC
ENAGSLKEMLAAGKVAAVTFTSSSTVRAFLDLLGDGALDLVQGIDVFCLGPVTAATAREA
CCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCHHHHHCCCCEEEECCHHHHHHHHC
GLQVAATAGEYTEEGLVRAMENYYTTIRAGGGDSNQSRSL
CCEEEECCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCC
>Mature Secondary Structure
MENTRGKVFLVGAGPGDPGLLTVKGRECLARAGAVVYDRLLNPALLEYAPPEAVKIYVGK
CCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECC
APDRHALSQDEINDLLVDLARQGKQVVRLKGGDPFVFGRGGEEALALRAAGIPFEVVPGV
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCEEEEEECCCCEEECCCC
TAAVAVPAYAGIPVTHRGLASTAAFITGNEDPRKGNSAINWEGLARAVDTLVFLMGMANL
HHHHHHHHHCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PYIVSRLLACGRSPDTPVALIRWGTRAEQETLTGTLADIEGRAQEAGFRNPAIIIIGQVV
HHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHCCCCCCEEEEEECHH
NLRSTLAWLEDKPLFGRRVIVTRPRAQAEGLTRSLADLGAEVINFPVIRTEPPADWHPLD
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHH
TALDAIGEFDWIIFTSANGVRYFWRRLLERHQDIRSLAGIRIAAIGPATSRALKERGLLT
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHCCCCC
DWQPREYVAEAVASGLGPRVRGRRVLLPRADIARPFLAVDLRRQGAEVTEVTAYRTVKNE
CCCHHHHHHHHHHHCCCCCCCCCEEECCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCC
ENAGSLKEMLAAGKVAAVTFTSSSTVRAFLDLLGDGALDLVQGIDVFCLGPVTAATAREA
CCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCHHHHHCCCCEEEECCHHHHHHHHC
GLQVAATAGEYTEEGLVRAMENYYTTIRAGGGDSNQSRSL
CCEEEECCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]