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The map label for this gene is pfp [H]

Identifier: 83313174

GI number: 83313174

Start: 4476656

End: 4477870

Strand: Reverse

Name: pfp [H]

Synonym: amb4075

Alternate gene names: 83313174

Gene position: 4477870-4476656 (Counterclockwise)

Preceding gene: 83313185

Following gene: 83313173

Centisome position: 90.15

GC content: 64.28

Gene sequence:

>1215_bases
ATGCTGCACGGCAAGGTTCTGGTGGCCCAGGGCGGCGGCCCGACGGCGGTGATCAACCAGTCCATGGTGGGGGCCGTCCT
GGAATCGCGCAAGTTCCGCAACGTGGATCTGGTTTACGGCGCCCATCACGGGGTGCGGGGGATCATCAACGAGGACTTCC
TCGATCTGACCCAGGAAACCAGCCACAACCTGGAAATGGTGGCGCAGACGCCGTCTTCGGCGCTGGGTTCGACGCGGGAC
AAGCCGGACCGCAAGTACTGCCACGAGATCTTCGAGGTGCTGCGCGCCCACGGCATCGCCTATTTCTTCTATATCGGCGG
CAACGATTCGTCGGACACCGTGCGCATCGTGTCCGAGGAGGCCAAGGCAGCCGATTACCCCCTGCGCTGCATCCACATCC
CCAAGACCATCGACAACGATCTGGTGCTGAACGACCACACGCCGGGCTTTCCCTCGGCCGCCCGCTTCGTGGCCCAGGCC
TTCATGGGGGTCAATCTGGACAATGCGGCGTTGCGCGGGGTCTATCTTGCCGTGGTGATGGGGCGCCATGCCGGTTTCCT
GACGGCGGCCTCGGCCCTGGGCAAGAAGTTTCCCGAAGACGGCCCCCACCTTATCTATATCCCCGAGCGGACGTTCCGGG
TGGAAAGCTTCCTCGCCGACGTCAAGGCGGCCTACGACAAGCATGGCCGCTGCGTGGTGGCCATTTCCGAAGGCGTGCAC
GACGAGAAGGGAACGCCCATCGTCACCACCCTGAGCAAGGCGACGGAAAAGGACGCCCACGGCAATATCCAGCTGTCGGG
GACCGGGGCGCTGTCCGACCTGCTGTGCGAGGAGGTGAAGGGCAAGCTTGGCATCAAGCGGGTGCGGGGCGACACCTTCG
GCTATCTCCAGCGCTCGTTCATCGGCTGCGTCTCGGACGTGGACCAGCGCGAGGCCCGCGAGGTGGGCGAAAAGGCAGTG
CAATTCGCCATGTGGGGCGATCAGGACGGCTCGGTGACCATCCAGCGCACCGGTTTCTACTCGGTGGATTACAAGCTGCT
GCCCCTGGCCTCGGTGGCCGGCAAGACAAGGGTGATGGAAGACCACTTCATCGCGGAGTCGGGCACCGACGTCACCGATG
CCTTCCGCATGTATCTGCGGCCGCTGCTGGGCTCGGGCATGCCCGACGCCTTCCGTCTGCGCCTCAATTCGGTGCCGAGG
ATTCTGAAGAAGTAG

Upstream 100 bases:

>100_bases
AGGCCTATCCTTTCGAGGCGGGGGGCTCCCCCTCATATTGGTCGCTGGCGGCCCGACAAAAAGGGGCTATCCTGAGACAG
CACCAAGAGGGAGGCGTTCC

Downstream 100 bases:

>100_bases
GGGGGATGCCACAAGCTTCTTGCCTCGGGCGCCGATTTGGCTATATTGACGCCAAGACACATTCTTGTATTCCCGTCAGA
CGGGATTGTGAGGGTGGGCC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MLHGKVLVAQGGGPTAVINQSMVGAVLESRKFRNVDLVYGAHHGVRGIINEDFLDLTQETSHNLEMVAQTPSSALGSTRD
KPDRKYCHEIFEVLRAHGIAYFFYIGGNDSSDTVRIVSEEAKAADYPLRCIHIPKTIDNDLVLNDHTPGFPSAARFVAQA
FMGVNLDNAALRGVYLAVVMGRHAGFLTAASALGKKFPEDGPHLIYIPERTFRVESFLADVKAAYDKHGRCVVAISEGVH
DEKGTPIVTTLSKATEKDAHGNIQLSGTGALSDLLCEEVKGKLGIKRVRGDTFGYLQRSFIGCVSDVDQREAREVGEKAV
QFAMWGDQDGSVTIQRTGFYSVDYKLLPLASVAGKTRVMEDHFIAESGTDVTDAFRMYLRPLLGSGMPDAFRLRLNSVPR
ILKK

Sequences:

>Translated_404_residues
MLHGKVLVAQGGGPTAVINQSMVGAVLESRKFRNVDLVYGAHHGVRGIINEDFLDLTQETSHNLEMVAQTPSSALGSTRD
KPDRKYCHEIFEVLRAHGIAYFFYIGGNDSSDTVRIVSEEAKAADYPLRCIHIPKTIDNDLVLNDHTPGFPSAARFVAQA
FMGVNLDNAALRGVYLAVVMGRHAGFLTAASALGKKFPEDGPHLIYIPERTFRVESFLADVKAAYDKHGRCVVAISEGVH
DEKGTPIVTTLSKATEKDAHGNIQLSGTGALSDLLCEEVKGKLGIKRVRGDTFGYLQRSFIGCVSDVDQREAREVGEKAV
QFAMWGDQDGSVTIQRTGFYSVDYKLLPLASVAGKTRVMEDHFIAESGTDVTDAFRMYLRPLLGSGMPDAFRLRLNSVPR
ILKK
>Mature_404_residues
MLHGKVLVAQGGGPTAVINQSMVGAVLESRKFRNVDLVYGAHHGVRGIINEDFLDLTQETSHNLEMVAQTPSSALGSTRD
KPDRKYCHEIFEVLRAHGIAYFFYIGGNDSSDTVRIVSEEAKAADYPLRCIHIPKTIDNDLVLNDHTPGFPSAARFVAQA
FMGVNLDNAALRGVYLAVVMGRHAGFLTAASALGKKFPEDGPHLIYIPERTFRVESFLADVKAAYDKHGRCVVAISEGVH
DEKGTPIVTTLSKATEKDAHGNIQLSGTGALSDLLCEEVKGKLGIKRVRGDTFGYLQRSFIGCVSDVDQREAREVGEKAV
QFAMWGDQDGSVTIQRTGFYSVDYKLLPLASVAGKTRVMEDHFIAESGTDVTDAFRMYLRPLLGSGMPDAFRLRLNSVPR
ILKK

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=269, Percent_Identity=26.7657992565056, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 44187; Mature: 44187

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHGKVLVAQGGGPTAVINQSMVGAVLESRKFRNVDLVYGAHHGVRGIINEDFLDLTQET
CCCCEEEEECCCCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHC
SHNLEMVAQTPSSALGSTRDKPDRKYCHEIFEVLRAHGIAYFFYIGGNDSSDTVRIVSEE
CCCEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEECC
AKAADYPLRCIHIPKTIDNDLVLNDHTPGFPSAARFVAQAFMGVNLDNAALRGVYLAVVM
CCCCCCCEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GRHAGFLTAASALGKKFPEDGPHLIYIPERTFRVESFLADVKAAYDKHGRCVVAISEGVH
HCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
DEKGTPIVTTLSKATEKDAHGNIQLSGTGALSDLLCEEVKGKLGIKRVRGDTFGYLQRSF
CCCCCEEEEEHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHH
IGCVSDVDQREAREVGEKAVQFAMWGDQDGSVTIQRTGFYSVDYKLLPLASVAGKTRVME
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCEEEECEEEEEHHHHCCCCEEHH
DHFIAESGTDVTDAFRMYLRPLLGSGMPDAFRLRLNSVPRILKK
HHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLHGKVLVAQGGGPTAVINQSMVGAVLESRKFRNVDLVYGAHHGVRGIINEDFLDLTQET
CCCCEEEEECCCCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHC
SHNLEMVAQTPSSALGSTRDKPDRKYCHEIFEVLRAHGIAYFFYIGGNDSSDTVRIVSEE
CCCEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEECC
AKAADYPLRCIHIPKTIDNDLVLNDHTPGFPSAARFVAQAFMGVNLDNAALRGVYLAVVM
CCCCCCCEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GRHAGFLTAASALGKKFPEDGPHLIYIPERTFRVESFLADVKAAYDKHGRCVVAISEGVH
HCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
DEKGTPIVTTLSKATEKDAHGNIQLSGTGALSDLLCEEVKGKLGIKRVRGDTFGYLQRSF
CCCCCEEEEEHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHH
IGCVSDVDQREAREVGEKAVQFAMWGDQDGSVTIQRTGFYSVDYKLLPLASVAGKTRVME
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCEEEECEEEEEHHHHCCCCEEHH
DHFIAESGTDVTDAFRMYLRPLLGSGMPDAFRLRLNSVPRILKK
HHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]