Click here to switch to the map view.

The map label for this gene is rnhB

Identifier: 83313088

GI number: 83313088

Start: 4399451

End: 4400056

Strand: Reverse

Name: rnhB

Synonym: amb3989

Alternate gene names: 83313088

Gene position: 4400056-4399451 (Counterclockwise)

Preceding gene: 83313089

Following gene: 83313087

Centisome position: 88.58

GC content: 68.98

Gene sequence:

>606_bases
ATGCCGGACCTGGCTCTGGAATTTGAGATCGGCGGCATCGTCTGCGGCATCGACGAGGTGGGCCGCGGCCCCCTGGCCGG
GCCGGTGGTGGCCGCCGCGGTGATCCTCGACCCCGCCCGCCTGCCCAAGACGCTGCTGGAGCGGCTGGACGATTCCAAGA
AGCTATCCAAGCGTAACCGCGAAGAACTGGCCGAACTGGTGCCCGCCACCGCCATCCTTGGCTTCGGCGAGGCTTCGGTG
GAGGAGATCGACCGGATCAACATCCTGCAGGCGACGTTTCTCGCCATGCGCCGCGCCTATGACGCCCTGGGGCGGGAATG
CGCCCATGCGCTGGTCGACGGCAACCGGCCGCCGGGGCTGCCCTGCCCGGTCCGCTGCGTGGTGGGGGGCGACGGCATCT
CATTGTCCATCGCCGCCGCCTCGGTGGTGGCCAAGGTGCGGCGCGACGCCATGATGGCCGATCTGGCCCGCGCCCACCCC
GAATTCGGCTGGGAGAGGAACGCCGGATACGGTACGGCCGAGCATCTGGACGCCCTGAAACGCCTCGGTCCCACCCCCCA
TCACCGACGCTCCTTCGCCCCCGTGGCCCAATATATGCTGTTCTAA

Upstream 100 bases:

>100_bases
CTGGTCACGCGGCTGTATGGCGACGAGGCCCGCGCTTTCGCCCGCACCGCGCGGCGGGCCCGTGTGCTGGTGCGGCGTGG
TTCCTGGAGGGATGACCCCA

Downstream 100 bases:

>100_bases
CCTGTGGATAAGCCACTACCTGTGGCAATGAATCATTAGATTCCAACAGGTTCTTGACGGACTCTCATGTTTGACTCAAA
TTGAGTGCTCCAACTCATTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MPDLALEFEIGGIVCGIDEVGRGPLAGPVVAAAVILDPARLPKTLLERLDDSKKLSKRNREELAELVPATAILGFGEASV
EEIDRINILQATFLAMRRAYDALGRECAHALVDGNRPPGLPCPVRCVVGGDGISLSIAAASVVAKVRRDAMMADLARAHP
EFGWERNAGYGTAEHLDALKRLGPTPHHRRSFAPVAQYMLF

Sequences:

>Translated_201_residues
MPDLALEFEIGGIVCGIDEVGRGPLAGPVVAAAVILDPARLPKTLLERLDDSKKLSKRNREELAELVPATAILGFGEASV
EEIDRINILQATFLAMRRAYDALGRECAHALVDGNRPPGLPCPVRCVVGGDGISLSIAAASVVAKVRRDAMMADLARAHP
EFGWERNAGYGTAEHLDALKRLGPTPHHRRSFAPVAQYMLF
>Mature_200_residues
PDLALEFEIGGIVCGIDEVGRGPLAGPVVAAAVILDPARLPKTLLERLDDSKKLSKRNREELAELVPATAILGFGEASVE
EIDRINILQATFLAMRRAYDALGRECAHALVDGNRPPGLPCPVRCVVGGDGISLSIAAASVVAKVRRDAMMADLARAHPE
FGWERNAGYGTAEHLDALKRLGPTPHHRRSFAPVAQYMLF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=51.0869565217391, Blast_Score=160, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_MAGSA (Q50412)

Other databases:

- EMBL:   D32253
- EMBL:   AP007255
- RefSeq:   YP_423352.1
- ProteinModelPortal:   Q50412
- SMR:   Q50412
- STRING:   Q50412
- GeneID:   3802894
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3989
- NMPDR:   fig|342108.5.peg.3583
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q50412
- ProtClustDB:   PRK00015
- BioCyc:   MMAG342108:AMB3989-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21597; Mature: 21466

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLALEFEIGGIVCGIDEVGRGPLAGPVVAAAVILDPARLPKTLLERLDDSKKLSKRNR
CCCCEEEEECCCEEECHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHH
EELAELVPATAILGFGEASVEEIDRINILQATFLAMRRAYDALGRECAHALVDGNRPPGL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PCPVRCVVGGDGISLSIAAASVVAKVRRDAMMADLARAHPEFGWERNAGYGTAEHLDALK
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
RLGPTPHHRRSFAPVAQYMLF
HCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PDLALEFEIGGIVCGIDEVGRGPLAGPVVAAAVILDPARLPKTLLERLDDSKKLSKRNR
CCCEEEEECCCEEECHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHH
EELAELVPATAILGFGEASVEEIDRINILQATFLAMRRAYDALGRECAHALVDGNRPPGL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PCPVRCVVGGDGISLSIAAASVVAKVRRDAMMADLARAHPEFGWERNAGYGTAEHLDALK
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
RLGPTPHHRRSFAPVAQYMLF
HCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499342