Click here to switch to the map view.

The map label for this gene is fixJ [H]

Identifier: 83312389

GI number: 83312389

Start: 3567136

End: 3567750

Strand: Reverse

Name: fixJ [H]

Synonym: amb3290

Alternate gene names: 83312389

Gene position: 3567750-3567136 (Counterclockwise)

Preceding gene: 83312390

Following gene: 83312388

Centisome position: 71.83

GC content: 66.18

Gene sequence:

>615_bases
GTGACCGATGCCTTGAAGCCCCTGGTCCTGGTGGTCGACGATGACGACGCCATGCGCGGTTCGGTGGCCTTTCTGCTGGC
CTCGGTGGGGCTGGACAGCCGGGGTTTCGCCACCGCCGAGGCGTTCCTGGCCCAGTTGCCCGATGACGGGCGGCCGGGCT
GCGTCATTCTCGATGTCCGCATGCCGGGCATGAGCGGCCTGGAATTGCAGCGCCTGCTGGCCGAGCGCAAATTCGTGCTG
CCCCTGGTCATCGTCACCGGCCATGGTGACGTGCCCATGGCGGTATCGGCGCTGAAGGCCGGCGCCCATGACTTCATCGA
GAAGCCCTTCAACGATCAGGTTCTGCTCGACGCCGTCACCGCCGCCATCCGGGCCAGCCGGGAAAAGCTCGCCGATGCGG
CCAACCGCGACGTGGTCGAAAAGCGTCTGGCCAGCCTGACCCGCCGCGAGCGCGAGGTGCTGGACCGGGTGCTGGCGGGC
AAGCCCAACAAGCAGATCGCCTTCGACCTGGATCTGTCCATCAAGACGGTGGAGGTTCACCGGCACGCGGTGATGGAAAA
GATGGCGGCCCGATCCGTGGCTGAACTGGCCCAATTGATGGTGGGCTTAGGGTAA

Upstream 100 bases:

>100_bases
GCACCATCATCGAGGCCCATGGCGGACGCCTGTGGGCCTCCGCCAATCCCCGGCGTGGCCTGACGCTGCGCTTCGTCATT
CCGCCAGCGGAGGCCCCAGC

Downstream 100 bases:

>100_bases
ACCCTAATTCCCCTGGGGGAGCCCCCAATACCCGCAAAATTCCAACGGCTATAGCCTGATCTCCGAGACCGATCCGCTTT
TCGGAGTTCCGGGCATGACC

Product: response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTDALKPLVLVVDDDDAMRGSVAFLLASVGLDSRGFATAEAFLAQLPDDGRPGCVILDVRMPGMSGLELQRLLAERKFVL
PLVIVTGHGDVPMAVSALKAGAHDFIEKPFNDQVLLDAVTAAIRASREKLADAANRDVVEKRLASLTRREREVLDRVLAG
KPNKQIAFDLDLSIKTVEVHRHAVMEKMAARSVAELAQLMVGLG

Sequences:

>Translated_204_residues
MTDALKPLVLVVDDDDAMRGSVAFLLASVGLDSRGFATAEAFLAQLPDDGRPGCVILDVRMPGMSGLELQRLLAERKFVL
PLVIVTGHGDVPMAVSALKAGAHDFIEKPFNDQVLLDAVTAAIRASREKLADAANRDVVEKRLASLTRREREVLDRVLAG
KPNKQIAFDLDLSIKTVEVHRHAVMEKMAARSVAELAQLMVGLG
>Mature_203_residues
TDALKPLVLVVDDDDAMRGSVAFLLASVGLDSRGFATAEAFLAQLPDDGRPGCVILDVRMPGMSGLELQRLLAERKFVLP
LVIVTGHGDVPMAVSALKAGAHDFIEKPFNDQVLLDAVTAAIRASREKLADAANRDVVEKRLASLTRREREVLDRVLAGK
PNKQIAFDLDLSIKTVEVHRHAVMEKMAARSVAELAQLMVGLG

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=126, Percent_Identity=36.5079365079365, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1790102, Length=165, Percent_Identity=34.5454545454545, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1788222, Length=205, Percent_Identity=25.3658536585366, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788712, Length=154, Percent_Identity=29.2207792207792, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=129, Percent_Identity=31.7829457364341, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788550, Length=103, Percent_Identity=31.0679611650485, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=32.1951219512195, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1787473, Length=196, Percent_Identity=31.6326530612245, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22032; Mature: 21901

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDALKPLVLVVDDDDAMRGSVAFLLASVGLDSRGFATAEAFLAQLPDDGRPGCVILDVR
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEE
MPGMSGLELQRLLAERKFVLPLVIVTGHGDVPMAVSALKAGAHDFIEKPFNDQVLLDAVT
CCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCHHHHHHCCCCCHHHHHHHH
AAIRASREKLADAANRDVVEKRLASLTRREREVLDRVLAGKPNKQIAFDLDLSIKTVEVH
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEHHHHH
RHAVMEKMAARSVAELAQLMVGLG
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDALKPLVLVVDDDDAMRGSVAFLLASVGLDSRGFATAEAFLAQLPDDGRPGCVILDVR
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEE
MPGMSGLELQRLLAERKFVLPLVIVTGHGDVPMAVSALKAGAHDFIEKPFNDQVLLDAVT
CCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCHHHHHHCCCCCHHHHHHHH
AAIRASREKLADAANRDVVEKRLASLTRREREVLDRVLAGKPNKQIAFDLDLSIKTVEVH
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEHHHHH
RHAVMEKMAARSVAELAQLMVGLG
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]