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The map label for this gene is fixL [H]

Identifier: 83312390

GI number: 83312390

Start: 3567747

End: 3569438

Strand: Reverse

Name: fixL [H]

Synonym: amb3291

Alternate gene names: 83312390

Gene position: 3569438-3567747 (Counterclockwise)

Preceding gene: 83312394

Following gene: 83312389

Centisome position: 71.86

GC content: 71.28

Gene sequence:

>1692_bases
GTGCTGGCCTTCCAGGGGGCCGAGCGCGCGCTCCGCACCTGGGAGCCTACGGCTGCGCACCTTAACCGCAGCGTGGGAGG
CAGCCGCTTCGTGCTGGTGCCTCTGGATCTCGACGCCATGAGCCGGGCGGTGGCCGGGCGCGAGGTGGATTTCGTCATCA
CCAATCCCGGCAATTACATCGCCCTGGAAAGCGGTTTCGGCGCCACCCGGCTGGTGACGGTGGAATCCGAGCGGAGCGGC
TCGCCCATCGCCGCCATCGGCTCGACGGTGGTGGCGCGGGGCGACCGGCCGGATCTCGACACCCTTGCCGGGCTGAAGGG
GCGGCGGCTGGCCGTGGTCTCCACCGAGGCGTTCGGCGGCTATCAGGTTCTGTGGCGCGAGCTGGCCGCCCTGGGCCTCG
ATCCGTCCGATGACCTGCGGGAAATCCGCCAGACCGGCTTTCCCATGGAACGGGTGGCCCAGTTGGTGCGGGACGGCTTG
GTCGATGCCGGGGTGCTGCGGGCCTGCCTGCTGGAGGAGATGATCGCCGAGGGCGCCCTGGCGCCGGGCGAGCTTCGGGT
GGTGGGGCGACGGGACGAGCCCAAGCTGCCCTGCGCCGTCTCGTCGCGGCTTTATCCCGACTGGCCGTTCGCCAAGCTGG
CCGAGACCTCCCATGATCTGGCCAAGCAGGTGGCGGCGGCGCTGTTGTCCATGCCGGCGGTGGACGGCCAGGCCTGGACG
GCGCCCCAGGACTACACCCAGGTGCACGCCCTGATGCAGGACCTGGAGATCGGCCCCTATGCCCATCTCCGCCAGCATCT
GAATCTGGCCGAACTGGCCCGCCGCCACTGGCAATGGCTGGTCATCGCCGCCATGGCGGTGCTGTGGTGGGTGGTGCATG
TGGCCCGTGTCGAGGTGCTGGTGCGCCGCCGCACCGCCGAGCTGGAACGCGAGATCGCCGAACGCGAGCGGGCCGAGAAC
GACGCCGCCCGCCACCGGGCCGAGCGCGACCAGTTCTCGCGGCTGGGCATCCTGGGGGAGATGGCCAGCAATATCGCCCA
CGAGCTGAACCAGCCCCTGGCGGCGATCATGAACTATGCCCGGGGCATGTCGCGCATGCTGGACGGCGGGCAGGCCGATC
CGGCCATGCTGGCCGACGGCGCCCAGGCGGTGGCCACCCAGGCCGAGCGGGCGGCGGCCATCATCCAGCGCATCCGGGGC
TTCGTCCGCCGGCGCAAGCCCCGGCGCGAGCGCCTGGACATCAACGAGGTGGTGGCCGAGACCCTGGCCCTGTTCGAATC
CCTGGCCGGACGGCGGGGCATCGCCGTGCATGTCCATCTGGCCGAGGGCCTGCCCGGCGTCTCGGTGGACCGGGGCGAGA
TTCAGCAGGTGCTGCTGAACATCTTGCAGAATGCCGTCGATGCCACGGCCGGGCAGGGGGCGGAGGCTGGCGACGGCATC
ACCGTGCGCACCTCCCCGGGCGAGGGCGGCGTCAAGGTGGCGGTGCGCGATTGCGGCGCCGGTCTCAGCCAGGAGGCCGA
AGCCCGGCTGTTCGAGCCGTTCTTCACCACCAAGCCGGAAGGGCTGGGCCTGGGCCTGTCCATCTGCCGCACCATCATCG
AGGCCCATGGCGGACGCCTGTGGGCCTCCGCCAATCCCCGGCGTGGCCTGACGCTGCGCTTCGTCATTCCGCCAGCGGAG
GCCCCAGCGTGA

Upstream 100 bases:

>100_bases
CTGCCCGTTTGGTCAGTCTCTTGCCATGGTTTTCCGGCTTTTCCTAATCTGCTGTCTGCTGACGTGCTCCGGCGCCTTTG
CCGCCGAGGTGCGGATCGGC

Downstream 100 bases:

>100_bases
CCGATGCCTTGAAGCCCCTGGTCCTGGTGGTCGACGATGACGACGCCATGCGCGGTTCGGTGGCCTTTCTGCTGGCCTCG
GTGGGGCTGGACAGCCGGGG

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MLAFQGAERALRTWEPTAAHLNRSVGGSRFVLVPLDLDAMSRAVAGREVDFVITNPGNYIALESGFGATRLVTVESERSG
SPIAAIGSTVVARGDRPDLDTLAGLKGRRLAVVSTEAFGGYQVLWRELAALGLDPSDDLREIRQTGFPMERVAQLVRDGL
VDAGVLRACLLEEMIAEGALAPGELRVVGRRDEPKLPCAVSSRLYPDWPFAKLAETSHDLAKQVAAALLSMPAVDGQAWT
APQDYTQVHALMQDLEIGPYAHLRQHLNLAELARRHWQWLVIAAMAVLWWVVHVARVEVLVRRRTAELEREIAERERAEN
DAARHRAERDQFSRLGILGEMASNIAHELNQPLAAIMNYARGMSRMLDGGQADPAMLADGAQAVATQAERAAAIIQRIRG
FVRRRKPRRERLDINEVVAETLALFESLAGRRGIAVHVHLAEGLPGVSVDRGEIQQVLLNILQNAVDATAGQGAEAGDGI
TVRTSPGEGGVKVAVRDCGAGLSQEAEARLFEPFFTTKPEGLGLGLSICRTIIEAHGGRLWASANPRRGLTLRFVIPPAE
APA

Sequences:

>Translated_563_residues
MLAFQGAERALRTWEPTAAHLNRSVGGSRFVLVPLDLDAMSRAVAGREVDFVITNPGNYIALESGFGATRLVTVESERSG
SPIAAIGSTVVARGDRPDLDTLAGLKGRRLAVVSTEAFGGYQVLWRELAALGLDPSDDLREIRQTGFPMERVAQLVRDGL
VDAGVLRACLLEEMIAEGALAPGELRVVGRRDEPKLPCAVSSRLYPDWPFAKLAETSHDLAKQVAAALLSMPAVDGQAWT
APQDYTQVHALMQDLEIGPYAHLRQHLNLAELARRHWQWLVIAAMAVLWWVVHVARVEVLVRRRTAELEREIAERERAEN
DAARHRAERDQFSRLGILGEMASNIAHELNQPLAAIMNYARGMSRMLDGGQADPAMLADGAQAVATQAERAAAIIQRIRG
FVRRRKPRRERLDINEVVAETLALFESLAGRRGIAVHVHLAEGLPGVSVDRGEIQQVLLNILQNAVDATAGQGAEAGDGI
TVRTSPGEGGVKVAVRDCGAGLSQEAEARLFEPFFTTKPEGLGLGLSICRTIIEAHGGRLWASANPRRGLTLRFVIPPAE
APA
>Mature_563_residues
MLAFQGAERALRTWEPTAAHLNRSVGGSRFVLVPLDLDAMSRAVAGREVDFVITNPGNYIALESGFGATRLVTVESERSG
SPIAAIGSTVVARGDRPDLDTLAGLKGRRLAVVSTEAFGGYQVLWRELAALGLDPSDDLREIRQTGFPMERVAQLVRDGL
VDAGVLRACLLEEMIAEGALAPGELRVVGRRDEPKLPCAVSSRLYPDWPFAKLAETSHDLAKQVAAALLSMPAVDGQAWT
APQDYTQVHALMQDLEIGPYAHLRQHLNLAELARRHWQWLVIAAMAVLWWVVHVARVEVLVRRRTAELEREIAERERAEN
DAARHRAERDQFSRLGILGEMASNIAHELNQPLAAIMNYARGMSRMLDGGQADPAMLADGAQAVATQAERAAAIIQRIRG
FVRRRKPRRERLDINEVVAETLALFESLAGRRGIAVHVHLAEGLPGVSVDRGEIQQVLLNILQNAVDATAGQGAEAGDGI
TVRTSPGEGGVKVAVRDCGAGLSQEAEARLFEPFFTTKPEGLGLGLSICRTIIEAHGGRLWASANPRRGLTLRFVIPPAE
APA

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=239, Percent_Identity=31.3807531380753, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=30.7359307359307, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1786912, Length=265, Percent_Identity=29.0566037735849, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1790300, Length=217, Percent_Identity=27.6497695852535, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788713, Length=237, Percent_Identity=27.4261603375527, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI48994928, Length=263, Percent_Identity=27.3764258555133, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789149, Length=266, Percent_Identity=25.187969924812, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87081816, Length=283, Percent_Identity=27.208480565371, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=24.5353159851301, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61080; Mature: 61080

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFQGAERALRTWEPTAAHLNRSVGGSRFVLVPLDLDAMSRAVAGREVDFVITNPGNYI
CCCCCCHHHHHHCCCCHHHHHHHCCCCCEEEEEEECHHHHHHHHCCCCEEEEEECCCCEE
ALESGFGATRLVTVESERSGSPIAAIGSTVVARGDRPDLDTLAGLKGRRLAVVSTEAFGG
EEECCCCCEEEEEEECCCCCCCHHHHCCHHEECCCCCCHHHHHCCCCCEEEEEECCCCCC
YQVLWRELAALGLDPSDDLREIRQTGFPMERVAQLVRDGLVDAGVLRACLLEEMIAEGAL
HHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
APGELRVVGRRDEPKLPCAVSSRLYPDWPFAKLAETSHDLAKQVAAALLSMPAVDGQAWT
CCCCEEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
APQDYTQVHALMQDLEIGPYAHLRQHLNLAELARRHWQWLVIAAMAVLWWVVHVARVEVL
CCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRRRTAELEREIAERERAENDAARHRAERDQFSRLGILGEMASNIAHELNQPLAAIMNYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RGMSRMLDGGQADPAMLADGAQAVATQAERAAAIIQRIRGFVRRRKPRRERLDINEVVAE
HHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHH
TLALFESLAGRRGIAVHVHLAEGLPGVSVDRGEIQQVLLNILQNAVDATAGQGAEAGDGI
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
TVRTSPGEGGVKVAVRDCGAGLSQEAEARLFEPFFTTKPEGLGLGLSICRTIIEAHGGRL
EEEECCCCCCEEEEEEHHCCCCCCHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHCCCEE
WASANPRRGLTLRFVIPPAEAPA
EECCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MLAFQGAERALRTWEPTAAHLNRSVGGSRFVLVPLDLDAMSRAVAGREVDFVITNPGNYI
CCCCCCHHHHHHCCCCHHHHHHHCCCCCEEEEEEECHHHHHHHHCCCCEEEEEECCCCEE
ALESGFGATRLVTVESERSGSPIAAIGSTVVARGDRPDLDTLAGLKGRRLAVVSTEAFGG
EEECCCCCEEEEEEECCCCCCCHHHHCCHHEECCCCCCHHHHHCCCCCEEEEEECCCCCC
YQVLWRELAALGLDPSDDLREIRQTGFPMERVAQLVRDGLVDAGVLRACLLEEMIAEGAL
HHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
APGELRVVGRRDEPKLPCAVSSRLYPDWPFAKLAETSHDLAKQVAAALLSMPAVDGQAWT
CCCCEEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
APQDYTQVHALMQDLEIGPYAHLRQHLNLAELARRHWQWLVIAAMAVLWWVVHVARVEVL
CCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRRRTAELEREIAERERAENDAARHRAERDQFSRLGILGEMASNIAHELNQPLAAIMNYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RGMSRMLDGGQADPAMLADGAQAVATQAERAAAIIQRIRGFVRRRKPRRERLDINEVVAE
HHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHH
TLALFESLAGRRGIAVHVHLAEGLPGVSVDRGEIQQVLLNILQNAVDATAGQGAEAGDGI
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
TVRTSPGEGGVKVAVRDCGAGLSQEAEARLFEPFFTTKPEGLGLGLSICRTIIEAHGGRL
EEEECCCCCCEEEEEEHHCCCCCCHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHCCCEE
WASANPRRGLTLRFVIPPAEAPA
EECCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]