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The map label for this gene is cheR [C]

Identifier: 83312031

GI number: 83312031

Start: 3129465

End: 3130886

Strand: Reverse

Name: cheR [C]

Synonym: amb2932

Alternate gene names: 83312031

Gene position: 3130886-3129465 (Counterclockwise)

Preceding gene: 83312032

Following gene: 83312030

Centisome position: 63.03

GC content: 66.39

Gene sequence:

>1422_bases
ATGACGGCGCCGCTTTACGTGGCGGGAATCGGCGCATCGGCCGGCGGGTTGGAGGCCATGCTGCTGATGTTCGCCCGGCT
GCGCCCCACCGGGCGCATCGCCTACGTGGTGGCCCAGCACATGGCCGATAACGGCCACAGCGATCTGGTGGTCAGGCTGA
TCCAGCGGGAATCCGCCCTGCCGGTCAGGCTGGCCGTCCATGGGAGCAGACTGCTGGCCGATACGGTGCTGGTCATTCCC
GCCGGCCGGGAGGGACATGTGGGCGACGGTGCGCTGGCGCTGCGCGATCCGGCACCCGAGCATCTATCGACTCCGTCGAT
CAATGCCTTGCTGACGTCCATCGCCGAGAGCTGTGGCCCGGCGGGGATCGGCATCATACTGTCCGGCGCCGGGTCCGACG
GTGTCGTCGGGTGCCGGTCGATCAAGAGCCGCGGCGGGACGACGCTGGCGCAGGAGCCGTCCGAGGCCAAGTTCGACGGC
ATGCCCAGTGCCGCCATCGGTGCCCGGCAGATCGATCAGGTCCTGTCGGCGGAAAAGATGGGGGACGCCCTGGCCGCGCG
TCTCTCCTGTTCGGCCCCCGCAGCCGGTGAGGTGGCCCATCTGGAGACGGCGACGGGAGCGGACCTGAATGAATTGCGGG
ATCTGGTCGCCAGGGTGCACCGGGCCACCGGCGTCGATTTTTCCAGCTACAAGGAGGAGACCCTGCTGCGTCGGCTGGAA
AAGCGGAAATCCATCCTGGGGATTGGGTCGGGCGCCGCCTATCTGGCGCTGATCCGCCGCGACCCCGAGGAATTACATGT
GCTGCAACGTCTGTTCCTGGTTTCGGTATCCTCGTTCTTTCGCGACCGGGACTCGTTTCGCGTGCTGGAGCGAGCCCTGG
CGGCGGCCCTGGCGGAAAAGCCCGCGTCGGAGCCTGTCCGGGTCTGGGTGCCCGGCTGCGCCTCGGGCGAGGAGGCCTTC
ACCCTGGCCATCGTCCTGAGGGAACTGGCCGGGCGTCTCGGCCGCGATCAGCCCATGGCCATCACCGCCACCGATCTCAA
TCCGGAGGCCCTGGCCATGGCCCGCGAGGGGCTCTATCGCAAGACGGCCTTCAAGGAGACGGAGGACATCCTGCGCGACC
GCTATTTCGTGGCGCGGGGGCAGCATTACGAGGTGACGCCGGAACTTCGAGCCGGCGTCACTTTCGAACGGCGCGACGTG
CTGGCCGGGGCGCCACCGGCCGATCTTGATCTGATAAGTTGCCGCAACCTATTGATTTATATGAAAAGTGCGCTGCAGGA
CAGCTTGGTGAAGACGTTTCATCAGGCTCTGAGGCCGTTTGGGTTGCTGTTCATCGGGCAATCCGAGTCCCTCAGCTTCA
TCGGGAATTCGTTGTTCGGCCCCGTCGACCACTACCACCGGCTGTTCCGTCGGCGCCGTTGA

Upstream 100 bases:

>100_bases
CGGGCACCGCGCTGGCCACCGCCTTGCAACCGGTGGCCAAGGCCATTGACGATTGCGACCTGGACGAAGCCCTGGTGCAG
CTCGGGAAGGTCAAGGTCCC

Downstream 100 bases:

>100_bases
ACGTCTGGAAAGGACCCTCCATGAAAGCCCTCTTGAATACCGTCGAGCGCCTCAGGCTCACCACCAAGCTGGTGATCGGC
TTTTCCGCGGGCATCCTGGT

Product: methylase of chemotaxis methyl-accepting protein

Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester; Tsrglu-Me; Trgglu-Me; Targlu-Me; Tapglu-Me + [C]

Alternate protein names: ORF19 [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTAPLYVAGIGASAGGLEAMLLMFARLRPTGRIAYVVAQHMADNGHSDLVVRLIQRESALPVRLAVHGSRLLADTVLVIP
AGREGHVGDGALALRDPAPEHLSTPSINALLTSIAESCGPAGIGIILSGAGSDGVVGCRSIKSRGGTTLAQEPSEAKFDG
MPSAAIGARQIDQVLSAEKMGDALAARLSCSAPAAGEVAHLETATGADLNELRDLVARVHRATGVDFSSYKEETLLRRLE
KRKSILGIGSGAAYLALIRRDPEELHVLQRLFLVSVSSFFRDRDSFRVLERALAAALAEKPASEPVRVWVPGCASGEEAF
TLAIVLRELAGRLGRDQPMAITATDLNPEALAMAREGLYRKTAFKETEDILRDRYFVARGQHYEVTPELRAGVTFERRDV
LAGAPPADLDLISCRNLLIYMKSALQDSLVKTFHQALRPFGLLFIGQSESLSFIGNSLFGPVDHYHRLFRRRR

Sequences:

>Translated_473_residues
MTAPLYVAGIGASAGGLEAMLLMFARLRPTGRIAYVVAQHMADNGHSDLVVRLIQRESALPVRLAVHGSRLLADTVLVIP
AGREGHVGDGALALRDPAPEHLSTPSINALLTSIAESCGPAGIGIILSGAGSDGVVGCRSIKSRGGTTLAQEPSEAKFDG
MPSAAIGARQIDQVLSAEKMGDALAARLSCSAPAAGEVAHLETATGADLNELRDLVARVHRATGVDFSSYKEETLLRRLE
KRKSILGIGSGAAYLALIRRDPEELHVLQRLFLVSVSSFFRDRDSFRVLERALAAALAEKPASEPVRVWVPGCASGEEAF
TLAIVLRELAGRLGRDQPMAITATDLNPEALAMAREGLYRKTAFKETEDILRDRYFVARGQHYEVTPELRAGVTFERRDV
LAGAPPADLDLISCRNLLIYMKSALQDSLVKTFHQALRPFGLLFIGQSESLSFIGNSLFGPVDHYHRLFRRRR
>Mature_472_residues
TAPLYVAGIGASAGGLEAMLLMFARLRPTGRIAYVVAQHMADNGHSDLVVRLIQRESALPVRLAVHGSRLLADTVLVIPA
GREGHVGDGALALRDPAPEHLSTPSINALLTSIAESCGPAGIGIILSGAGSDGVVGCRSIKSRGGTTLAQEPSEAKFDGM
PSAAIGARQIDQVLSAEKMGDALAARLSCSAPAAGEVAHLETATGADLNELRDLVARVHRATGVDFSSYKEETLLRRLEK
RKSILGIGSGAAYLALIRRDPEELHVLQRLFLVSVSSFFRDRDSFRVLERALAAALAEKPASEPVRVWVPGCASGEEAFT
LAIVLRELAGRLGRDQPMAITATDLNPEALAMAREGLYRKTAFKETEDILRDRYFVARGQHYEVTPELRAGVTFERRDVL
AGAPPADLDLISCRNLLIYMKSALQDSLVKTFHQALRPFGLLFIGQSESLSFIGNSLFGPVDHYHRLFRRRR

Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788193, Length=253, Percent_Identity=28.8537549407115, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788192, Length=184, Percent_Identity=27.7173913043478, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]

EC number: 2.1.1.80 [C]

Molecular weight: Translated: 50986; Mature: 50855

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50123 CHER ; PS50122 CHEB ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLYVAGIGASAGGLEAMLLMFARLRPTGRIAYVVAQHMADNGHSDLVVRLIQRESAL
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
PVRLAVHGSRLLADTVLVIPAGREGHVGDGALALRDPAPEHLSTPSINALLTSIAESCGP
CEEEEECCCHHHCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCC
AGIGIILSGAGSDGVVGCRSIKSRGGTTLAQEPSEAKFDGMPSAAIGARQIDQVLSAEKM
CCEEEEEECCCCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GDALAARLSCSAPAAGEVAHLETATGADLNELRDLVARVHRATGVDFSSYKEETLLRRLE
HHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KRKSILGIGSGAAYLALIRRDPEELHVLQRLFLVSVSSFFRDRDSFRVLERALAAALAEK
HHHHHEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
PASEPVRVWVPGCASGEEAFTLAIVLRELAGRLGRDQPMAITATDLNPEALAMAREGLYR
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHH
KTAFKETEDILRDRYFVARGQHYEVTPELRAGVTFERRDVLAGAPPADLDLISCRNLLIY
HHHHHHHHHHHHHHHHHCCCCCEEECCHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHH
MKSALQDSLVKTFHQALRPFGLLFIGQSESLSFIGNSLFGPVDHYHRLFRRRR
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TAPLYVAGIGASAGGLEAMLLMFARLRPTGRIAYVVAQHMADNGHSDLVVRLIQRESAL
CCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
PVRLAVHGSRLLADTVLVIPAGREGHVGDGALALRDPAPEHLSTPSINALLTSIAESCGP
CEEEEECCCHHHCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCC
AGIGIILSGAGSDGVVGCRSIKSRGGTTLAQEPSEAKFDGMPSAAIGARQIDQVLSAEKM
CCEEEEEECCCCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GDALAARLSCSAPAAGEVAHLETATGADLNELRDLVARVHRATGVDFSSYKEETLLRRLE
HHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KRKSILGIGSGAAYLALIRRDPEELHVLQRLFLVSVSSFFRDRDSFRVLERALAAALAEK
HHHHHEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
PASEPVRVWVPGCASGEEAFTLAIVLRELAGRLGRDQPMAITATDLNPEALAMAREGLYR
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHH
KTAFKETEDILRDRYFVARGQHYEVTPELRAGVTFERRDVLAGAPPADLDLISCRNLLIY
HHHHHHHHHHHHHHHHHCCCCCEEECCHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHH
MKSALQDSLVKTFHQALRPFGLLFIGQSESLSFIGNSLFGPVDHYHRLFRRRR
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): 10 [C]

Specific activity: NA

Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]

Substrates: S-Adenosyl-L-methionine; protein L-glutamate; Tsrglu; Trgglu; Targlu; Tapglu [C]

Specific reaction: S-Adenosyl-L-methionine + protein L-glutamate =S-adenosyl-L-homocysteine + protein L-glutamate methyl ester S-adenosyl-L-methionine + Tsrglu = S-adenosyl-homocysteine + Tsrglu-Me S-adenosyl-L-methionine + Trgglu = S-adenosyl-homocysteine + Trgglu-Me S-ade

General reaction: Methyl group transfer [C]

Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]