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The map label for this gene is hup [H]

Identifier: 83311888

GI number: 83311888

Start: 2976598

End: 2977008

Strand: Reverse

Name: hup [H]

Synonym: amb2789

Alternate gene names: 83311888

Gene position: 2977008-2976598 (Counterclockwise)

Preceding gene: 83311889

Following gene: 83311887

Centisome position: 59.93

GC content: 60.34

Gene sequence:

>411_bases
GTGGATGCCAGCAATTCCGCCATTTCTTGCCGATTGCGCATTGACGCTGTCCGACAGCCTGAATTAGACTGCCCCCTGTC
TAATTCGATAGGCGGTATGGCCGGCTATCAATGTCGGTATAACGAAGGGGGTCCTCAGGTGAACAAGAACGATCTCGTGA
CGGCCGTGGCTGGCGCCGCTGGTCTGTCCAAGGCCGATGCGGCTAAGGCCGTTGATGGTGTTTTCGAAGCCATTACCTCC
GCGCTGAAGGGCGGCGACGAGGTTCGTCTGGTCGGTTTCGGTACTTTTGCCGTGGCCTCGCGCGCCGCGTCCGAGGGCCG
CAACCCGCGTACCGGCGAAAAGATCAGCATTCCGGCGTCCAAGCAGCCGAAGTTCAAGGCCGGCAAGGGCCTGAAGGACG
GCATCCAGTAA

Upstream 100 bases:

>100_bases
GACGCACTGATACCGATAAGGGTAATTCCCTGGCGGAAACCGGCGGTTCTCGATGGGAGCCGCCGGTTCTGTTTGGGGTG
GGTGCGGGGTGTCGTGTGGC

Downstream 100 bases:

>100_bases
GGATGTCTCCATGCTGAAAGTACGATGTGCCGGCCGCCTTCAGGTGGCCGGTTTTGTTTTGGGGGCTTGCCGAAAGTGCT
CCGGCGTCCTAGAACCCCCC

Product: nucleoid DNA-binding protein

Products: NA

Alternate protein names: DNA-binding protein II; HB [H]

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MDASNSAISCRLRIDAVRQPELDCPLSNSIGGMAGYQCRYNEGGPQVNKNDLVTAVAGAAGLSKADAAKAVDGVFEAITS
ALKGGDEVRLVGFGTFAVASRAASEGRNPRTGEKISIPASKQPKFKAGKGLKDGIQ

Sequences:

>Translated_136_residues
MDASNSAISCRLRIDAVRQPELDCPLSNSIGGMAGYQCRYNEGGPQVNKNDLVTAVAGAAGLSKADAAKAVDGVFEAITS
ALKGGDEVRLVGFGTFAVASRAASEGRNPRTGEKISIPASKQPKFKAGKGLKDGIQ
>Mature_136_residues
MDASNSAISCRLRIDAVRQPELDCPLSNSIGGMAGYQCRYNEGGPQVNKNDLVTAVAGAAGLSKADAAKAVDGVFEAITS
ALKGGDEVRLVGFGTFAVASRAASEGRNPRTGEKISIPASKQPKFKAGKGLKDGIQ

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=52.8089887640449, Blast_Score=97, Evalue=3e-22,
Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=48.8888888888889, Blast_Score=87, Evalue=4e-19,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=37.7777777777778, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 13983; Mature: 13983

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASNSAISCRLRIDAVRQPELDCPLSNSIGGMAGYQCRYNEGGPQVNKNDLVTAVAGAA
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCC
GLSKADAAKAVDGVFEAITSALKGGDEVRLVGFGTFAVASRAASEGRNPRTGEKISIPAS
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCEEECCCC
KQPKFKAGKGLKDGIQ
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDASNSAISCRLRIDAVRQPELDCPLSNSIGGMAGYQCRYNEGGPQVNKNDLVTAVAGAA
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCC
GLSKADAAKAVDGVFEAITSALKGGDEVRLVGFGTFAVASRAASEGRNPRTGEKISIPAS
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCEEECCCC
KQPKFKAGKGLKDGIQ
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644313 [H]