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The map label for this gene is birA [H]

Identifier: 83311872

GI number: 83311872

Start: 2958729

End: 2959475

Strand: Reverse

Name: birA [H]

Synonym: amb2773

Alternate gene names: 83311872

Gene position: 2959475-2958729 (Counterclockwise)

Preceding gene: 83311873

Following gene: 83311871

Centisome position: 59.58

GC content: 70.68

Gene sequence:

>747_bases
ATGACAACCTTCGATCTGCCGGCGCCGTTCAGCCATGTGGAGCTGGACAGCGTCGGCAGCACCAATGACGAGGTTCGGCG
GCGGGTCGAGGACGGCTCCGCCGCCGATCTTTTGGTGGTGACGGCCCGGCGCCAGACCTCGGGGCGCGGCCGACGCGGCC
GGGTGTGGGACAGCCCCGAGGGCAACCTGCACGTTTCCTTCGCCATCCGGATCCAGAGGCCGCTGTCCGAGGCAGCCCAG
ATCGGCTTCGCGGCGGCGCTGGCCCTGGCCGAGGCCCTTGACGAAGTGGTGCCGGGGCACGATGTGCGATGCAAGTGGCC
CAACGACGTGCTGGTCGACGGGCGCAAGGTGGCGGGCATGCTGCTGGAGAGCGCCGGTGACGGCTGGTTGATTCTGGGCA
TCGGCGTCGACGTGGAACGCCGCCCGGCGCCGGCCGAGGCTTTGTATGCCGCCGTCGCGCTGGCCGAGTTGGGCTATGGC
GGCGATAGCATGGGCGTGCTGGCTGCGCTGTGTCGGCATCTCGGCCCCTGGCTGGAGCGCTGGCGGGCCGACGGCTTTGC
CCCCATCAGGGCCGGCTGGCTGGAGTGGGCGAGGGGCCTGGGCGAGGCGGCGCTGGTCCGCCTCGAGGCCGAGACGCTGT
CCGGCGTGTTCCGGGGCCTCGACGAGGAAGGCGGTCTGCTGCTCGATCAGGGCGAGGCCGGAATCCGGCGGGTTCTGGCT
GGGGATGTGTTTTTTCCGGGATTGTGA

Upstream 100 bases:

>100_bases
TGGCGGTTTCCACCATCGTCATCCTAGCCTTTACCCTGATCCCGGCCCCGCTGGTGACCAGCGCCAAGGCCGCCGCCCAG
GTTCTGTTCCCGGCGGCGGG

Downstream 100 bases:

>100_bases
CGGAGCTTTTCCATGCTGTTGGCCATCGATTCGGGCAACACCAATATCGTCTTCGCGGTCTATGACGGCGACACCCTGCG
CGGCGAGTGGCGTGCCTCGA

Product: biotin-(acetyl-CoA carboxylase) ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTTFDLPAPFSHVELDSVGSTNDEVRRRVEDGSAADLLVVTARRQTSGRGRRGRVWDSPEGNLHVSFAIRIQRPLSEAAQ
IGFAAALALAEALDEVVPGHDVRCKWPNDVLVDGRKVAGMLLESAGDGWLILGIGVDVERRPAPAEALYAAVALAELGYG
GDSMGVLAALCRHLGPWLERWRADGFAPIRAGWLEWARGLGEAALVRLEAETLSGVFRGLDEEGGLLLDQGEAGIRRVLA
GDVFFPGL

Sequences:

>Translated_248_residues
MTTFDLPAPFSHVELDSVGSTNDEVRRRVEDGSAADLLVVTARRQTSGRGRRGRVWDSPEGNLHVSFAIRIQRPLSEAAQ
IGFAAALALAEALDEVVPGHDVRCKWPNDVLVDGRKVAGMLLESAGDGWLILGIGVDVERRPAPAEALYAAVALAELGYG
GDSMGVLAALCRHLGPWLERWRADGFAPIRAGWLEWARGLGEAALVRLEAETLSGVFRGLDEEGGLLLDQGEAGIRRVLA
GDVFFPGL
>Mature_247_residues
TTFDLPAPFSHVELDSVGSTNDEVRRRVEDGSAADLLVVTARRQTSGRGRRGRVWDSPEGNLHVSFAIRIQRPLSEAAQI
GFAAALALAEALDEVVPGHDVRCKWPNDVLVDGRKVAGMLLESAGDGWLILGIGVDVERRPAPAEALYAAVALAELGYGG
DSMGVLAALCRHLGPWLERWRADGFAPIRAGWLEWARGLGEAALVRLEAETLSGVFRGLDEEGGLLLDQGEAGIRRVLAG
DVFFPGL

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=223, Percent_Identity=30.9417040358744, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 26584; Mature: 26453

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFDLPAPFSHVELDSVGSTNDEVRRRVEDGSAADLLVVTARRQTSGRGRRGRVWDSPE
CCCCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCC
GNLHVSFAIRIQRPLSEAAQIGFAAALALAEALDEVVPGHDVRCKWPNDVLVDGRKVAGM
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCHHHHHH
LLESAGDGWLILGIGVDVERRPAPAEALYAAVALAELGYGGDSMGVLAALCRHLGPWLER
HHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
WRADGFAPIRAGWLEWARGLGEAALVRLEAETLSGVFRGLDEEGGLLLDQGEAGIRRVLA
HHCCCCCHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHH
GDVFFPGL
CCCCCCCC
>Mature Secondary Structure 
TTFDLPAPFSHVELDSVGSTNDEVRRRVEDGSAADLLVVTARRQTSGRGRRGRVWDSPE
CCCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCC
GNLHVSFAIRIQRPLSEAAQIGFAAALALAEALDEVVPGHDVRCKWPNDVLVDGRKVAGM
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCHHHHHH
LLESAGDGWLILGIGVDVERRPAPAEALYAAVALAELGYGGDSMGVLAALCRHLGPWLER
HHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
WRADGFAPIRAGWLEWARGLGEAALVRLEAETLSGVFRGLDEEGGLLLDQGEAGIRRVLA
HHCCCCCHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHH
GDVFFPGL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]