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The map label for this gene is tmk [H]

Identifier: 83311549

GI number: 83311549

Start: 2626633

End: 2627271

Strand: Reverse

Name: tmk [H]

Synonym: amb2450

Alternate gene names: 83311549

Gene position: 2627271-2626633 (Counterclockwise)

Preceding gene: 83311550

Following gene: 83311548

Centisome position: 52.89

GC content: 69.64

Gene sequence:

>639_bases
ATGGAGACAGCTCGCGGACGCTTCATCACCTTCGAGGGCGGAGAAGGCGCGGGCAAATCCACCCAGGTCCGACTGCTGGC
GGAATCCCTGCGCGACGAAGGCCTGATGGTGGTCACCACCCGCGAGCCCGGCGGCTCGGCCGGGGCGGAGGCCATCCGTG
CCCTGCTGGTGGAGGGCGAACCCGAGCGCTGGGACGGAGTTACCGAGGCGCTGCTCCATTTCGCGGCGCGCCGCGATCAT
CTGGTCAAGACGGTGTGGCCGGCGCTGGACCGCGGCGCCTGGGTCATCTGCGACCGTTTCGCCGATTCCACCCTGGCCTA
TCAGGGCTTCGGCCACGGCCTCGACCCCGACGTGATCGCCAGCCTGTATCGGGTGGCCGTGGGTCATTTCGCCCCCGACT
TGACCCTGGTCCTCGATCTGCCGGTGGAAAAGGGGCTGGAGCGGGCGGGGCTGAGGGGCGGCGCCGAAGACCGCTACGAG
CGCATGGGCCTGGGGTTTCACCAGCGCCTGCGCCAGGGATTCCTGGGGATTGCCGCCGCCAATCCCATGCGCTGCGCCGT
CATCGACGCCACCCGGTCGCCTGACGAAGTGCACGGCGACATCATGTCCACGGTCAGGGCGCGGCTGCCGGTGCCATGA

Upstream 100 bases:

>100_bases
TTCCCATGGTGGCCGGTGCCGACGTGGAGAAGCTGGGCTTTGCCGGGCGCATGTCCACCGCCTTCAAGCGCGTCATCTGG
GGCAAGAAGGGTTAACAGGC

Downstream 100 bases:

>100_bases
CCGACTCTCCACACCCTCGCGAGACCTCCGAGCTGTTCGGCCACGGAGCGGCCGAGTCGGCGCTGCTGGATGCCTGGAAC
TCGGGCCGGCTGGCCCATGC

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
METARGRFITFEGGEGAGKSTQVRLLAESLRDEGLMVVTTREPGGSAGAEAIRALLVEGEPERWDGVTEALLHFAARRDH
LVKTVWPALDRGAWVICDRFADSTLAYQGFGHGLDPDVIASLYRVAVGHFAPDLTLVLDLPVEKGLERAGLRGGAEDRYE
RMGLGFHQRLRQGFLGIAAANPMRCAVIDATRSPDEVHGDIMSTVRARLPVP

Sequences:

>Translated_212_residues
METARGRFITFEGGEGAGKSTQVRLLAESLRDEGLMVVTTREPGGSAGAEAIRALLVEGEPERWDGVTEALLHFAARRDH
LVKTVWPALDRGAWVICDRFADSTLAYQGFGHGLDPDVIASLYRVAVGHFAPDLTLVLDLPVEKGLERAGLRGGAEDRYE
RMGLGFHQRLRQGFLGIAAANPMRCAVIDATRSPDEVHGDIMSTVRARLPVP
>Mature_212_residues
METARGRFITFEGGEGAGKSTQVRLLAESLRDEGLMVVTTREPGGSAGAEAIRALLVEGEPERWDGVTEALLHFAARRDH
LVKTVWPALDRGAWVICDRFADSTLAYQGFGHGLDPDVIASLYRVAVGHFAPDLTLVLDLPVEKGLERAGLRGGAEDRYE
RMGLGFHQRLRQGFLGIAAANPMRCAVIDATRSPDEVHGDIMSTVRARLPVP

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=205, Percent_Identity=41.9512195121951, Blast_Score=124, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 23030; Mature: 23030

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METARGRFITFEGGEGAGKSTQVRLLAESLRDEGLMVVTTREPGGSAGAEAIRALLVEGE
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCC
PERWDGVTEALLHFAARRDHLVKTVWPALDRGAWVICDRFADSTLAYQGFGHGLDPDVIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCHHHHH
SLYRVAVGHFAPDLTLVLDLPVEKGLERAGLRGGAEDRYERMGLGFHQRLRQGFLGIAAA
HHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHEEECC
NPMRCAVIDATRSPDEVHGDIMSTVRARLPVP
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METARGRFITFEGGEGAGKSTQVRLLAESLRDEGLMVVTTREPGGSAGAEAIRALLVEGE
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCC
PERWDGVTEALLHFAARRDHLVKTVWPALDRGAWVICDRFADSTLAYQGFGHGLDPDVIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCHHHHH
SLYRVAVGHFAPDLTLVLDLPVEKGLERAGLRGGAEDRYERMGLGFHQRLRQGFLGIAAA
HHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHEEECC
NPMRCAVIDATRSPDEVHGDIMSTVRARLPVP
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]