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The map label for this gene is gatB

Identifier: 83310683

GI number: 83310683

Start: 1713376

End: 1714833

Strand: Reverse

Name: gatB

Synonym: amb1584

Alternate gene names: 83310683

Gene position: 1714833-1713376 (Counterclockwise)

Preceding gene: 83310684

Following gene: 83310682

Centisome position: 34.52

GC content: 64.68

Gene sequence:

>1458_bases
ATGGCGTACATCATTCAGGGCGAAACCGGCGATTGGGAGATCGTCATCGGTCTCGAGGTTCATGCCCAGGTCATCTCCAA
GGCCAAGCTGTTCAGTGGCGCCGCCACCGAATTCGGCGCCGAGCCCAACACCCAGGTGAGCTTCATCGACGCCGGCTTCC
CCGGCATGCTGCCGGTGATCAACGAAGTCTGCGTCGAGCAGGCGGTGCGCACCGGTCTGGGCCTCAAGGCCAAGATCAAC
CTGACCTCGGTCTTCGCCCGCAAGAACTACTTCTACGCCGACCTGCCCCAGGGCTATCAGATCAGCCAGTACGACCAGCC
CATCGTGGGCGAAGGCGAGTTGATCCTCGACCTGCCCGACGGCGGCACCCGCACCGTGGGCATCGAGCGCCTGCACCTGG
AGCAGGACGCCGGCAAGTCCATCCACGACATGCACCCGTCCAAGTCGTTCATCGACCTCAACCGTTCGGGCGTGGCGCTG
ATGGAGATCGTCTCCAAGCCCGACCTGCGCTCGCCGGAAGAGGCGGGCCTGTACCTCACCAAGCTGCGCTCCATCCTGCG
TTATCTCGAGACCTGCGACGGCAACATGCAGGAAGGCAGCATGCGCTGCGATGCCAACGTCTCGGTGCGGCCCGTGGGCT
CGACCGAGCTGCGCACCCGCTGCGAGATCAAGAACGTCAACTCCATCCGCTTCGTGCAGCAGGCCATCGAATTCGAAGCC
CGGCGCCACATCGACGTCTACGAGAGCGGCGGCGAGATCCGCCAGGAAACCCGCCTGTTCGATTCGGTCAAGGGCGAGAC
GCGGTCCATGCGCTCCAAGGAGCATGCCCACGACTATCGCTACTTCCCCGACCCCGACCTGCTGCCCCTGGTGATCGAGC
AGTCCTTCGTGGACGGCATCAAGTCCGGCCTGCCCGAACTGCCCGACGAGAAGAAGGCCCGCTTCATGGCGGAATACGGC
CTCAACGCCTATGACGCCGGCGTGCTGGTGGCGGAAAAGGCCTCGGCCGAGTTCTTCGAGACCGTGGCCAAGGGCCGCGA
TTCCAAGATGGCCTGCAATTGGGTGATGGGCGACTTCTTCGCGTCGCTGAACGCCACGGGCAAGACCATCGAGAACCCGC
CGGTCACCGCCGCCAATCTCGGCCAACTCATCGACCTGATCAAGGACGGCACGCTGTCGGGCCGCCTCGCCAAGGACGTC
TTCGCCCTGATGGTCGAGACCGGCAAGGCCCCCGCCGTCCTGGTCGAGGAACGCGGCCTGAAGCAGGTCACCGACACCGG
CGCCATCGAGGCGGCGGTGGACGCGGTGATCAGCGCCAATCCGGACAAGGTCCAGCAGGTCAAGGACGGCAAGACCGCCC
TGCTCGGCTGGTTCACCGGCCAGGTGATGAAGTCCACCCAGGGCAAGGCCAACCCGGCCATGGTCAACGAGATGTTGTCC
AAGAAGATCCTGGGGTAG

Upstream 100 bases:

>100_bases
CTGATCGGGCGTCCCTTCGACGAAGAGACGGTGTTCCGCGTGGCGGGCGTGATGGAAACGGCCGCGAACTTCACCGCCAA
GCCGGAAGGGGTGTAAGACC

Downstream 100 bases:

>100_bases
GTTTCCCACGCTTTGTCGCGTCCCCGACCTCCATGTCGGGGATGCGACAAAGCCGAAACCCCTTAATTTCCGCCATTTCT
CGCGATGCAGCACTTGGCAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MAYIIQGETGDWEIVIGLEVHAQVISKAKLFSGAATEFGAEPNTQVSFIDAGFPGMLPVINEVCVEQAVRTGLGLKAKIN
LTSVFARKNYFYADLPQGYQISQYDQPIVGEGELILDLPDGGTRTVGIERLHLEQDAGKSIHDMHPSKSFIDLNRSGVAL
MEIVSKPDLRSPEEAGLYLTKLRSILRYLETCDGNMQEGSMRCDANVSVRPVGSTELRTRCEIKNVNSIRFVQQAIEFEA
RRHIDVYESGGEIRQETRLFDSVKGETRSMRSKEHAHDYRYFPDPDLLPLVIEQSFVDGIKSGLPELPDEKKARFMAEYG
LNAYDAGVLVAEKASAEFFETVAKGRDSKMACNWVMGDFFASLNATGKTIENPPVTAANLGQLIDLIKDGTLSGRLAKDV
FALMVETGKAPAVLVEERGLKQVTDTGAIEAAVDAVISANPDKVQQVKDGKTALLGWFTGQVMKSTQGKANPAMVNEMLS
KKILG

Sequences:

>Translated_485_residues
MAYIIQGETGDWEIVIGLEVHAQVISKAKLFSGAATEFGAEPNTQVSFIDAGFPGMLPVINEVCVEQAVRTGLGLKAKIN
LTSVFARKNYFYADLPQGYQISQYDQPIVGEGELILDLPDGGTRTVGIERLHLEQDAGKSIHDMHPSKSFIDLNRSGVAL
MEIVSKPDLRSPEEAGLYLTKLRSILRYLETCDGNMQEGSMRCDANVSVRPVGSTELRTRCEIKNVNSIRFVQQAIEFEA
RRHIDVYESGGEIRQETRLFDSVKGETRSMRSKEHAHDYRYFPDPDLLPLVIEQSFVDGIKSGLPELPDEKKARFMAEYG
LNAYDAGVLVAEKASAEFFETVAKGRDSKMACNWVMGDFFASLNATGKTIENPPVTAANLGQLIDLIKDGTLSGRLAKDV
FALMVETGKAPAVLVEERGLKQVTDTGAIEAAVDAVISANPDKVQQVKDGKTALLGWFTGQVMKSTQGKANPAMVNEMLS
KKILG
>Mature_484_residues
AYIIQGETGDWEIVIGLEVHAQVISKAKLFSGAATEFGAEPNTQVSFIDAGFPGMLPVINEVCVEQAVRTGLGLKAKINL
TSVFARKNYFYADLPQGYQISQYDQPIVGEGELILDLPDGGTRTVGIERLHLEQDAGKSIHDMHPSKSFIDLNRSGVALM
EIVSKPDLRSPEEAGLYLTKLRSILRYLETCDGNMQEGSMRCDANVSVRPVGSTELRTRCEIKNVNSIRFVQQAIEFEAR
RHIDVYESGGEIRQETRLFDSVKGETRSMRSKEHAHDYRYFPDPDLLPLVIEQSFVDGIKSGLPELPDEKKARFMAEYGL
NAYDAGVLVAEKASAEFFETVAKGRDSKMACNWVMGDFFASLNATGKTIENPPVTAANLGQLIDLIKDGTLSGRLAKDVF
ALMVETGKAPAVLVEERGLKQVTDTGAIEAAVDAVISANPDKVQQVKDGKTALLGWFTGQVMKSTQGKANPAMVNEMLSK
KILG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=494, Percent_Identity=42.3076923076923, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI115534094, Length=310, Percent_Identity=40, Blast_Score=201, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6319391, Length=511, Percent_Identity=28.9628180039139, Blast_Score=209, Evalue=6e-55,
Organism=Drosophila melanogaster, GI45550808, Length=488, Percent_Identity=36.0655737704918, Blast_Score=321, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_MAGSA (Q2W6Y7)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_420947.1
- ProteinModelPortal:   Q2W6Y7
- SMR:   Q2W6Y7
- STRING:   Q2W6Y7
- GeneID:   3802392
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb1584
- NMPDR:   fig|342108.5.peg.1411
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q2W6Y7
- ProtClustDB:   PRK05477
- BioCyc:   MMAG342108:AMB1584-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53132; Mature: 53001

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYIIQGETGDWEIVIGLEVHAQVISKAKLFSGAATEFGAEPNTQVSFIDAGFPGMLPVI
CEEEEECCCCCEEEEEEEHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCHHHHH
NEVCVEQAVRTGLGLKAKINLTSVFARKNYFYADLPQGYQISQYDQPIVGEGELILDLPD
HHHHHHHHHHHCCCCEEEEEHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCC
GGTRTVGIERLHLEQDAGKSIHDMHPSKSFIDLNRSGVALMEIVSKPDLRSPEEAGLYLT
CCCEEEEEEEEEECHHCCCCHHHCCCCCCEEECCCCCHHHHHHHCCCCCCCCHHHCHHHH
KLRSILRYLETCDGNMQEGSMRCDANVSVRPVGSTELRTRCEIKNVNSIRFVQQAIEFEA
HHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCCHHHHHEEECCCCHHHHHHHHHHHHHH
RRHIDVYESGGEIRQETRLFDSVKGETRSMRSKEHAHDYRYFPDPDLLPLVIEQSFVDGI
HCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
KSGLPELPDEKKARFMAEYGLNAYDAGVLVAEKASAEFFETVAKGRDSKMACNWVMGDFF
HHCCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHCCCCCCEEHHHHHHHHH
ASLNATGKTIENPPVTAANLGQLIDLIKDGTLSGRLAKDVFALMVETGKAPAVLVEERGL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCC
KQVTDTGAIEAAVDAVISANPDKVQQVKDGKTALLGWFTGQVMKSTQGKANPAMVNEMLS
HHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHH
KKILG
HHCCC
>Mature Secondary Structure 
AYIIQGETGDWEIVIGLEVHAQVISKAKLFSGAATEFGAEPNTQVSFIDAGFPGMLPVI
EEEEECCCCCEEEEEEEHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCHHHHH
NEVCVEQAVRTGLGLKAKINLTSVFARKNYFYADLPQGYQISQYDQPIVGEGELILDLPD
HHHHHHHHHHHCCCCEEEEEHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCC
GGTRTVGIERLHLEQDAGKSIHDMHPSKSFIDLNRSGVALMEIVSKPDLRSPEEAGLYLT
CCCEEEEEEEEEECHHCCCCHHHCCCCCCEEECCCCCHHHHHHHCCCCCCCCHHHCHHHH
KLRSILRYLETCDGNMQEGSMRCDANVSVRPVGSTELRTRCEIKNVNSIRFVQQAIEFEA
HHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCCHHHHHEEECCCCHHHHHHHHHHHHHH
RRHIDVYESGGEIRQETRLFDSVKGETRSMRSKEHAHDYRYFPDPDLLPLVIEQSFVDGI
HCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
KSGLPELPDEKKARFMAEYGLNAYDAGVLVAEKASAEFFETVAKGRDSKMACNWVMGDFF
HHCCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHCCCCCCEEHHHHHHHHH
ASLNATGKTIENPPVTAANLGQLIDLIKDGTLSGRLAKDVFALMVETGKAPAVLVEERGL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCC
KQVTDTGAIEAAVDAVISANPDKVQQVKDGKTALLGWFTGQVMKSTQGKANPAMVNEMLS
HHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHH
KKILG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA