Click here to switch to the map view.

The map label for this gene is nifB [H]

Identifier: 83310682

GI number: 83310682

Start: 1711731

End: 1713227

Strand: Reverse

Name: nifB [H]

Synonym: amb1583

Alternate gene names: 83310682

Gene position: 1713227-1711731 (Counterclockwise)

Preceding gene: 83310683

Following gene: 83310681

Centisome position: 34.49

GC content: 65.4

Gene sequence:

>1497_bases
GTGTCCAGCAACGTCATTTCTCTCTCCAGCATCGCCCGTGGACCCATGGTCCCCGCCGCGACCCCGGCCGCGCCCACCGG
CGGCTGTTCCTCCAGCTCGTGCGGGTCCGACAAGGGCCAGGGCGACATGCCCGCGCATATCTGGGACAAGGTCAAGGACC
ACCCGTGCTATTCGGAAGAAGCGCACCACTACTTCGCCCGCATGCATGTGGCGGTGGCTCCGGCCTGCAACATCCAATGC
AATTACTGTAACCGGAAATACGACTGCGCCAACGAATCGCGCCCCGGCGTCACCAGCGAGAAGCTGACGCCGGAACAGGC
GGCGCGCAAGGTGGCGGCGGTGGCGGCCAAGGTGCCGCAGCTTTCGGTTCTGGGCATCGCCGGTCCCGGCGATTCCATGT
TCGACTGGAAGAAGACGGCGGCCACCTTCCGCGAGGTGTCCAAGCTGCTGCCCGACCTCAAATACTGCGTCTCGACCAAC
GGTCTGGCCCTGCCCGACCACCTGGACGAGCTGGCCGGGCACAACATCGACCACGTCACCATCACCATCAACATGGTCGA
CCCCGAGATCGGCGCGCTGATCTATCCCTGGATCTACTTCAAGGGCAAGCGCTACACCGGGATCGAAGGCGCCCGCATCC
TGCACGAGCGCCAGATGGAAGGCCTGGAAGGCCTGACGTCGCGGGGCATCCTCACCAAGATCAACTCGGTGATGATCCCC
GGGATCAACGACCAGCACCTGATCGAGGTCAACAAGGAGATCAAGGCGCGGGGTGCTTTCCTGCATAACGTCATGCCGCT
GATTTCCGACGCCGCCCACGGCACCGCCTTCGGCCTGTCCGGCCAGCGTGGCCCCAAGCCCGCCGAACTGAAGGACCTTC
AGGACAAGCTGGCCGGCGGCGCCAATCTCATGCGCCATTGCCGCCAGTGCCGCGCCGACGCGGTGGGCATGCTGGGCGAG
GATCTGTCCCAGGATTTCACCCTGGACAAGCTGCCGGAAGTGGCCACCGCCTACGACCCCGAGCCGCGTCGCCTGTACCG
CGAGGTGGTCGAGCGCGAACGCGCCGACCGCGCCAAGGCCCTGGAAGGTGCCGAGGCCGAACTGGCCGAAGCCGTCGCCG
AAATCGAGGCCCCGGCCAAGCTGGTCGCCGTCTGCACCAAGGGCGGCGGGCGCATCAATCAGCACTTCGGCCACGCCAAG
GAATTCCAGATCTTCGAAGTGGACGCCAAGGGCGTGCGCTTCTCGGGCCACCGCAAGGCCGACAATTACTGTCAGGGCGG
CTATGGCGACGACGACAAGCTGGATGTTGTACTGGCCGCGCTGGAAGGCATCGAGACGGTGTTCGTGGCCAAGATCGGCC
GCTGCCCCAAGGACGACCTGAAGAAGGCCGGCATCGAAGCGGTGGACGCCTACCCCTTCGAATACATCGAGACGGCGGTG
GCCGGCTGGTACGCCCAGCGCTTCGGGCAGGTGTCCGATACCGCCGACATTGCTTAA

Upstream 100 bases:

>100_bases
AAAGCCGAAACCCCTTAATTTCCGCCATTTCTCGCGATGCAGCACTTGGCATCGTCATTGCAAAGGTATCCGGCATAGAC
CCCTGACGAGGAGGCCGCCG

Downstream 100 bases:

>100_bases
GAGAGAAGAGGAGAAAAATCATGGCTCTGAAGATCATCGCTTCCGAATGCACCACCTGCGGCGCCTGTGAGTTCGAGTGC
CCCAACGCCGCCATCTCCAT

Product: FeMo cofactor biosynthesis protein nifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSSNVISLSSIARGPMVPAATPAAPTGGCSSSSCGSDKGQGDMPAHIWDKVKDHPCYSEEAHHYFARMHVAVAPACNIQC
NYCNRKYDCANESRPGVTSEKLTPEQAARKVAAVAAKVPQLSVLGIAGPGDSMFDWKKTAATFREVSKLLPDLKYCVSTN
GLALPDHLDELAGHNIDHVTITINMVDPEIGALIYPWIYFKGKRYTGIEGARILHERQMEGLEGLTSRGILTKINSVMIP
GINDQHLIEVNKEIKARGAFLHNVMPLISDAAHGTAFGLSGQRGPKPAELKDLQDKLAGGANLMRHCRQCRADAVGMLGE
DLSQDFTLDKLPEVATAYDPEPRRLYREVVERERADRAKALEGAEAELAEAVAEIEAPAKLVAVCTKGGGRINQHFGHAK
EFQIFEVDAKGVRFSGHRKADNYCQGGYGDDDKLDVVLAALEGIETVFVAKIGRCPKDDLKKAGIEAVDAYPFEYIETAV
AGWYAQRFGQVSDTADIA

Sequences:

>Translated_498_residues
MSSNVISLSSIARGPMVPAATPAAPTGGCSSSSCGSDKGQGDMPAHIWDKVKDHPCYSEEAHHYFARMHVAVAPACNIQC
NYCNRKYDCANESRPGVTSEKLTPEQAARKVAAVAAKVPQLSVLGIAGPGDSMFDWKKTAATFREVSKLLPDLKYCVSTN
GLALPDHLDELAGHNIDHVTITINMVDPEIGALIYPWIYFKGKRYTGIEGARILHERQMEGLEGLTSRGILTKINSVMIP
GINDQHLIEVNKEIKARGAFLHNVMPLISDAAHGTAFGLSGQRGPKPAELKDLQDKLAGGANLMRHCRQCRADAVGMLGE
DLSQDFTLDKLPEVATAYDPEPRRLYREVVERERADRAKALEGAEAELAEAVAEIEAPAKLVAVCTKGGGRINQHFGHAK
EFQIFEVDAKGVRFSGHRKADNYCQGGYGDDDKLDVVLAALEGIETVFVAKIGRCPKDDLKKAGIEAVDAYPFEYIETAV
AGWYAQRFGQVSDTADIA
>Mature_497_residues
SSNVISLSSIARGPMVPAATPAAPTGGCSSSSCGSDKGQGDMPAHIWDKVKDHPCYSEEAHHYFARMHVAVAPACNIQCN
YCNRKYDCANESRPGVTSEKLTPEQAARKVAAVAAKVPQLSVLGIAGPGDSMFDWKKTAATFREVSKLLPDLKYCVSTNG
LALPDHLDELAGHNIDHVTITINMVDPEIGALIYPWIYFKGKRYTGIEGARILHERQMEGLEGLTSRGILTKINSVMIPG
INDQHLIEVNKEIKARGAFLHNVMPLISDAAHGTAFGLSGQRGPKPAELKDLQDKLAGGANLMRHCRQCRADAVGMLGED
LSQDFTLDKLPEVATAYDPEPRRLYREVVERERADRAKALEGAEAELAEAVAEIEAPAKLVAVCTKGGGRINQHFGHAKE
FQIFEVDAKGVRFSGHRKADNYCQGGYGDDDKLDVVLAALEGIETVFVAKIGRCPKDDLKKAGIEAVDAYPFEYIETAVA
GWYAQRFGQVSDTADIA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53975; Mature: 53844

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNVISLSSIARGPMVPAATPAAPTGGCSSSSCGSDKGQGDMPAHIWDKVKDHPCYSEE
CCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHH
AHHYFARMHVAVAPACNIQCNYCNRKYDCANESRPGVTSEKLTPEQAARKVAAVAAKVPQ
HHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
LSVLGIAGPGDSMFDWKKTAATFREVSKLLPDLKYCVSTNGLALPDHLDELAGHNIDHVT
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEE
ITINMVDPEIGALIYPWIYFKGKRYTGIEGARILHERQMEGLEGLTSRGILTKINSVMIP
EEEEEECCCCCCEEEEHEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEC
GINDQHLIEVNKEIKARGAFLHNVMPLISDAAHGTAFGLSGQRGPKPAELKDLQDKLAGG
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHCCC
ANLMRHCRQCRADAVGMLGEDLSQDFTLDKLPEVATAYDPEPRRLYREVVERERADRAKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LEGAEAELAEAVAEIEAPAKLVAVCTKGGGRINQHFGHAKEFQIFEVDAKGVRFSGHRKA
HCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCEEEEEECCCCCEECCCCCC
DNYCQGGYGDDDKLDVVLAALEGIETVFVAKIGRCPKDDLKKAGIEAVDAYPFEYIETAV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHCCCHHHHHHHH
AGWYAQRFGQVSDTADIA
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SSNVISLSSIARGPMVPAATPAAPTGGCSSSSCGSDKGQGDMPAHIWDKVKDHPCYSEE
CCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHH
AHHYFARMHVAVAPACNIQCNYCNRKYDCANESRPGVTSEKLTPEQAARKVAAVAAKVPQ
HHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
LSVLGIAGPGDSMFDWKKTAATFREVSKLLPDLKYCVSTNGLALPDHLDELAGHNIDHVT
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEE
ITINMVDPEIGALIYPWIYFKGKRYTGIEGARILHERQMEGLEGLTSRGILTKINSVMIP
EEEEEECCCCCCEEEEHEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEC
GINDQHLIEVNKEIKARGAFLHNVMPLISDAAHGTAFGLSGQRGPKPAELKDLQDKLAGG
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHCCC
ANLMRHCRQCRADAVGMLGEDLSQDFTLDKLPEVATAYDPEPRRLYREVVERERADRAKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LEGAEAELAEAVAEIEAPAKLVAVCTKGGGRINQHFGHAKEFQIFEVDAKGVRFSGHRKA
HCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCEEEEEECCCCCEECCCCCC
DNYCQGGYGDDDKLDVVLAALEGIETVFVAKIGRCPKDDLKKAGIEAVDAYPFEYIETAV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHCCCHHHHHHHH
AGWYAQRFGQVSDTADIA
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]