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The map label for this gene is gatA

Identifier: 83310684

GI number: 83310684

Start: 1714838

End: 1716103

Strand: Reverse

Name: gatA

Synonym: amb1585

Alternate gene names: 83310684

Gene position: 1716103-1714838 (Counterclockwise)

Preceding gene: 83310689

Following gene: 83310683

Centisome position: 34.55

GC content: 67.22

Gene sequence:

>1266_bases
ATGGACGGTCTGCCCATCGGCATCAAGGACCTGTTCTGTACCGAAGGCGTGCAGACCACGGCGGCCAGCCACATCCTGGA
AGGCTTCAAGCCGCCGTACGAGTCCACCGTCTCGGGCAAGCTGAAGGCCGCCGGCGCCATTTCGCTGGGCAAGCTCAACC
TCGACGAATTCGCCATGGGCTCGTCCAACCAGACCAGCTATTTCGGCGCGGTGGAGAATCCCTGGAAGAAGACCTCCGAT
CCCAAGGCCAAGCTGGTTCCCGGCGGTTCCTCCGGCGGTTCGGCGGCGGCCGTGGCGGCGCGCATGGTGCTGGGTGCCAC
CGGCACCGACACCGGCGGCTCCATCCGCCAGCCCGCCGCCTTCTGCGGCATCACCGGCATCAAGCCGACCTACGGCCGCT
GCTCGCGCTTCGGCATCGTGGCCTTCGCCTCGTCCCTGGATCAGGCCGGCCCCATGGCCCGCACGGTGCGCGACTGCGCC
ATCATGCTGGGCGCCATGGCCGGGCATGACCCCAAGGATTCCACCTCGGTCAACATGGCCGTGCCGGATTTCGAACGTGC
TCTGACCGGCGACATCCGCGGCCTGAAGGTGGGCATTCCCAAGGAATACCGCCCCGACGGCCTGTCCGACGAGGTGGCCA
AGGTTTGGGACCGGGGCATCGAGTGGCTGAAGGCCGCCGGCGCCACGCCGGTGGAGATCAGCCTGCCGCACACCAAGTAC
GCGCTGGCCACCTATTACATCATCGCGCCGGCCGAGTGCTCGTCCAACCTGGCGCGCTATGACGGGTTGCGCTATGGCCT
CCGGGTGCCGGGCAAGACCTTGGACGACATGTACAAGAAAAGCCGTGCCGCCGGCTTCGGCGCCGAGGTTCGCCGCCGCA
TCCTGATCGGCACCTATGTGCTGTCGGCGGGCTATTACGACGCCTATTACGCCAAGGCCCAGAAGGTCCGGCGTCTGATC
GCCGAGGACTTCCGCAAGGCCTTCGAAACGGTCGACGTCATCCTCACCCCCACCGCGCCGTCGGCGGCGTTCGGCATGGG
CGAGGGCACTGACGATCCGGTGACCATGTGGTTGAACGATGTCTTCACCATCCCCACCAGCATGGCCGGTCTGCCCGGCC
TGTCGCTGCCCGCCGGCCTGTCCGCCGACGGGTTGCCGCTGGGCCTGCAGCTGATCGGGCGTCCCTTCGACGAAGAGACG
GTGTTCCGCGTGGCGGGCGTGATGGAAACGGCCGCGAACTTCACCGCCAAGCCGGAAGGGGTGTAA

Upstream 100 bases:

>100_bases
CCAGCGCCATCTCAACGCCTTCATCGTCGAGACCCCCGACCTCGCCCTGGAGGCCGCCAAGGCCTCGGACGCGCGCCGTC
AGGCGGGTACGGCCGGGTCC

Downstream 100 bases:

>100_bases
GACCATGGCGTACATCATTCAGGGCGAAACCGGCGATTGGGAGATCGTCATCGGTCTCGAGGTTCATGCCCAGGTCATCT
CCAAGGCCAAGCTGTTCAGT

Product: Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidase

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MDGLPIGIKDLFCTEGVQTTAASHILEGFKPPYESTVSGKLKAAGAISLGKLNLDEFAMGSSNQTSYFGAVENPWKKTSD
PKAKLVPGGSSGGSAAAVAARMVLGATGTDTGGSIRQPAAFCGITGIKPTYGRCSRFGIVAFASSLDQAGPMARTVRDCA
IMLGAMAGHDPKDSTSVNMAVPDFERALTGDIRGLKVGIPKEYRPDGLSDEVAKVWDRGIEWLKAAGATPVEISLPHTKY
ALATYYIIAPAECSSNLARYDGLRYGLRVPGKTLDDMYKKSRAAGFGAEVRRRILIGTYVLSAGYYDAYYAKAQKVRRLI
AEDFRKAFETVDVILTPTAPSAAFGMGEGTDDPVTMWLNDVFTIPTSMAGLPGLSLPAGLSADGLPLGLQLIGRPFDEET
VFRVAGVMETAANFTAKPEGV

Sequences:

>Translated_421_residues
MDGLPIGIKDLFCTEGVQTTAASHILEGFKPPYESTVSGKLKAAGAISLGKLNLDEFAMGSSNQTSYFGAVENPWKKTSD
PKAKLVPGGSSGGSAAAVAARMVLGATGTDTGGSIRQPAAFCGITGIKPTYGRCSRFGIVAFASSLDQAGPMARTVRDCA
IMLGAMAGHDPKDSTSVNMAVPDFERALTGDIRGLKVGIPKEYRPDGLSDEVAKVWDRGIEWLKAAGATPVEISLPHTKY
ALATYYIIAPAECSSNLARYDGLRYGLRVPGKTLDDMYKKSRAAGFGAEVRRRILIGTYVLSAGYYDAYYAKAQKVRRLI
AEDFRKAFETVDVILTPTAPSAAFGMGEGTDDPVTMWLNDVFTIPTSMAGLPGLSLPAGLSADGLPLGLQLIGRPFDEET
VFRVAGVMETAANFTAKPEGV
>Mature_421_residues
MDGLPIGIKDLFCTEGVQTTAASHILEGFKPPYESTVSGKLKAAGAISLGKLNLDEFAMGSSNQTSYFGAVENPWKKTSD
PKAKLVPGGSSGGSAAAVAARMVLGATGTDTGGSIRQPAAFCGITGIKPTYGRCSRFGIVAFASSLDQAGPMARTVRDCA
IMLGAMAGHDPKDSTSVNMAVPDFERALTGDIRGLKVGIPKEYRPDGLSDEVAKVWDRGIEWLKAAGATPVEISLPHTKY
ALATYYIIAPAECSSNLARYDGLRYGLRVPGKTLDDMYKKSRAAGFGAEVRRRILIGTYVLSAGYYDAYYAKAQKVRRLI
AEDFRKAFETVDVILTPTAPSAAFGMGEGTDDPVTMWLNDVFTIPTSMAGLPGLSLPAGLSADGLPLGLQLIGRPFDEET
VFRVAGVMETAANFTAKPEGV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=433, Percent_Identity=45.2655889145497, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI195972892, Length=430, Percent_Identity=27.2093023255814, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI166795287, Length=158, Percent_Identity=33.5443037974684, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=412, Percent_Identity=40.5339805825243, Blast_Score=305, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17537465, Length=428, Percent_Identity=25.7009345794392, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=131, Percent_Identity=33.587786259542, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=205, Percent_Identity=30.2439024390244, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=421, Percent_Identity=37.0546318289786, Blast_Score=246, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=422, Percent_Identity=30.0947867298578, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648113, Length=428, Percent_Identity=42.2897196261682, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24652985, Length=439, Percent_Identity=25.2847380410023, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19922090, Length=439, Percent_Identity=25.2847380410023, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24652981, Length=439, Percent_Identity=25.2847380410023, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24652983, Length=439, Percent_Identity=25.2847380410023, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI161078093, Length=436, Percent_Identity=25.4587155963303, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644968, Length=426, Percent_Identity=23.2394366197183, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21356731, Length=429, Percent_Identity=24.4755244755245, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_MAGSA (Q2W6Y6)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_420948.1
- ProteinModelPortal:   Q2W6Y6
- SMR:   Q2W6Y6
- STRING:   Q2W6Y6
- GeneID:   3802393
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb1585
- NMPDR:   fig|342108.5.peg.1412
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q2W6Y6
- BioCyc:   MMAG342108:AMB1585-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 44464; Mature: 44464

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 159-159 ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGLPIGIKDLFCTEGVQTTAASHILEGFKPPYESTVSGKLKAAGAISLGKLNLDEFAMG
CCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEECCCCHHHHCCC
SSNQTSYFGAVENPWKKTSDPKAKLVPGGSSGGSAAAVAARMVLGATGTDTGGSIRQPAA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHH
FCGITGIKPTYGRCSRFGIVAFASSLDQAGPMARTVRDCAIMLGAMAGHDPKDSTSVNMA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VPDFERALTGDIRGLKVGIPKEYRPDGLSDEVAKVWDRGIEWLKAAGATPVEISLPHTKY
CCCHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCE
ALATYYIIAPAECSSNLARYDGLRYGLRVPGKTLDDMYKKSRAAGFGAEVRRRILIGTYV
EEEEEEEEECHHHHCCHHHHCCCEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LSAGYYDAYYAKAQKVRRLIAEDFRKAFETVDVILTPTAPSAAFGMGEGTDDPVTMWLND
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHCCCCCCCCCCHHHHHHH
VFTIPTSMAGLPGLSLPAGLSADGLPLGLQLIGRPFDEETVFRVAGVMETAANFTAKPEG
HHHCCHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
V
C
>Mature Secondary Structure
MDGLPIGIKDLFCTEGVQTTAASHILEGFKPPYESTVSGKLKAAGAISLGKLNLDEFAMG
CCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEECCCCHHHHCCC
SSNQTSYFGAVENPWKKTSDPKAKLVPGGSSGGSAAAVAARMVLGATGTDTGGSIRQPAA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHH
FCGITGIKPTYGRCSRFGIVAFASSLDQAGPMARTVRDCAIMLGAMAGHDPKDSTSVNMA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VPDFERALTGDIRGLKVGIPKEYRPDGLSDEVAKVWDRGIEWLKAAGATPVEISLPHTKY
CCCHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCE
ALATYYIIAPAECSSNLARYDGLRYGLRVPGKTLDDMYKKSRAAGFGAEVRRRILIGTYV
EEEEEEEEECHHHHCCHHHHCCCEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LSAGYYDAYYAKAQKVRRLIAEDFRKAFETVDVILTPTAPSAAFGMGEGTDDPVTMWLND
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHCCCCCCCCCCHHHHHHH
VFTIPTSMAGLPGLSLPAGLSADGLPLGLQLIGRPFDEETVFRVAGVMETAANFTAKPEG
HHHCCHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA