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The map label for this gene is ppsB [H]

Identifier: 83310459

GI number: 83310459

Start: 1475043

End: 1475846

Strand: Reverse

Name: ppsB [H]

Synonym: amb1360

Alternate gene names: 83310459

Gene position: 1475846-1475043 (Counterclockwise)

Preceding gene: 83310460

Following gene: 83310458

Centisome position: 29.71

GC content: 70.27

Gene sequence:

>804_bases
GTGACACAATCCCTGCCTTCCGCCCTGTTCGGGGCCCGCGATTCCTCGCTGATCCGTGACGAGACCCTGTGGGAGATCTT
CTCCACCAGCGCCAGGACCTTCCCCAGCGCCGCCGCCGCCACCTTCGGCGACACCACCTTGACCTATGCCCAGCTGGCGG
AGCGCGCCCAGGCCGTGGCCCGTGCCCTGGCGGCGCGCGGCATCGGACGCGGCGATTTCGTCGGCCTGTGGATGAGCCGG
TCGCTGGATCTGCATGTGGCGCTGCTGGGCATCCTGGCCGCCGGCGCCGCCTATATCCCCTTCGACACCGAAGCCCCCGC
CGAGCGCGTCGCCGAATGCCTGGATGATTGCGCCGCCAAGGCGCTGATCGTCGACGCCTTCACCATGGGCGCCATCACCG
GCGCCATGCCGGCCCATACCCTGATTCTGCCGACCCTGGAGCAGGGCGCCCCCGGCGGCGCCTCGCCCGACCCGCGCGCC
CAGGGCGCCAATCCGGCCGACCCGGCCTATGCCATCTACACCTCGGGCTCCACCGGCAAGCCCAAGGCCATCGTCATCAG
CCATTTCAACATCTGCCACTACCTGCGCGCCGCCAATTCCCTTTACGGCCTGCGCTCCGACGACGTGGTGTTCCAGGGCG
CCTCGGTGGCCTTCGACCTGTCGCTGGAGGAGATCTTCGTCCCCTATCTGGTGGGCGCCAAGCTGTGGATCGCCGGCCGG
CGCACCTGGCCGAGGCCGACCGCCTGCCGCAGGTGCTGGCCGAGGCCGGCATCACCGTGCTGGACACCGTGCCGACCCTG
CTGA

Upstream 100 bases:

>100_bases
TGGGGGGTGACCGCTTTGCCGTGAGTTGGTATACCCTCCCCATGGACGAGGCCTTGGCCATCGCCGCGCGCGCCGAAGCC
GCGCCCAACCTGGAAATGAC

Downstream 100 bases:

>100_bases
GCCTGCTGCCCGGCGACATTCCCGGCCTGCGGGTCATCATCCTGGGCGGCGAGGCCTGCCCCGCGGCCCTGGCCGAGCGC
TGGTGCCGGCCCGGACGGCG

Product: Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: Peptide synthase 2; ATP-dependent tyrosine adenylase 1; TyrA 1; Tyrosine activase 1; ATP-dependent threonine adenylase; ThrA; Threonine activase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTQSLPSALFGARDSSLIRDETLWEIFSTSARTFPSAAAATFGDTTLTYAQLAERAQAVARALAARGIGRGDFVGLWMSR
SLDLHVALLGILAAGAAYIPFDTEAPAERVAECLDDCAAKALIVDAFTMGAITGAMPAHTLILPTLEQGAPGGASPDPRA
QGANPADPAYAIYTSGSTGKPKAIVISHFNICHYLRAANSLYGLRSDDVVFQGASVAFDLSLEEIFVPYLVGAKLWIAGR
RTWPRPTACRRCWPRPASPCWTPCRPC

Sequences:

>Translated_267_residues
MTQSLPSALFGARDSSLIRDETLWEIFSTSARTFPSAAAATFGDTTLTYAQLAERAQAVARALAARGIGRGDFVGLWMSR
SLDLHVALLGILAAGAAYIPFDTEAPAERVAECLDDCAAKALIVDAFTMGAITGAMPAHTLILPTLEQGAPGGASPDPRA
QGANPADPAYAIYTSGSTGKPKAIVISHFNICHYLRAANSLYGLRSDDVVFQGASVAFDLSLEEIFVPYLVGAKLWIAGR
RTWPRPTACRRCWPRPASPCWTPCRPC
>Mature_266_residues
TQSLPSALFGARDSSLIRDETLWEIFSTSARTFPSAAAATFGDTTLTYAQLAERAQAVARALAARGIGRGDFVGLWMSRS
LDLHVALLGILAAGAAYIPFDTEAPAERVAECLDDCAAKALIVDAFTMGAITGAMPAHTLILPTLEQGAPGGASPDPRAQ
GANPADPAYAIYTSGSTGKPKAIVISHFNICHYLRAANSLYGLRSDDVVFQGASVAFDLSLEEIFVPYLVGAKLWIAGRR
TWPRPTACRRCWPRPASPCWTPCRPC

Specific function: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Tyr and Thr as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The Thr residue is further converted to the D-allo-isomer form. The activation sit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI227499619, Length=209, Percent_Identity=30.1435406698565, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI40807357, Length=210, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1786801, Length=236, Percent_Identity=31.7796610169492, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648676, Length=230, Percent_Identity=29.5652173913043, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 28404; Mature: 28272

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSLPSALFGARDSSLIRDETLWEIFSTSARTFPSAAAATFGDTTLTYAQLAERAQAVA
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHH
RALAARGIGRGDFVGLWMSRSLDLHVALLGILAAGAAYIPFDTEAPAERVAECLDDCAAK
HHHHHCCCCCCCEEEEEHHCCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHH
ALIVDAFTMGAITGAMPAHTLILPTLEQGAPGGASPDPRAQGANPADPAYAIYTSGSTGK
HHHHHHHHHHHHHCCCCCCEEEEECHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
PKAIVISHFNICHYLRAANSLYGLRSDDVVFQGASVAFDLSLEEIFVPYLVGAKLWIAGR
CCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCHHHHHHHHHHCCEEEEECC
RTWPRPTACRRCWPRPASPCWTPCRPC
CCCCCCHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQSLPSALFGARDSSLIRDETLWEIFSTSARTFPSAAAATFGDTTLTYAQLAERAQAVA
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHH
RALAARGIGRGDFVGLWMSRSLDLHVALLGILAAGAAYIPFDTEAPAERVAECLDDCAAK
HHHHHCCCCCCCEEEEEHHCCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHH
ALIVDAFTMGAITGAMPAHTLILPTLEQGAPGGASPDPRAQGANPADPAYAIYTSGSTGK
HHHHHHHHHHHHHCCCCCCEEEEECHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
PKAIVISHFNICHYLRAANSLYGLRSDDVVFQGASVAFDLSLEEIFVPYLVGAKLWIAGR
CCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCHHHHHHHHHHCCEEEEECC
RTWPRPTACRRCWPRPASPCWTPCRPC
CCCCCCHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7711903; 9384377; 10471562 [H]