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The map label for this gene is glmU

Identifier: 83310379

GI number: 83310379

Start: 1396778

End: 1398073

Strand: Reverse

Name: glmU

Synonym: amb1280

Alternate gene names: 83310379

Gene position: 1398073-1396778 (Counterclockwise)

Preceding gene: 83310382

Following gene: 83310378

Centisome position: 28.15

GC content: 68.06

Gene sequence:

>1296_bases
ATGAAGTCGTCGCTCCCCAAGGTGATGCACCCGCTGGCGGGACGGCCCATGGTGAGCCATCTGCTCGACACCGTGTCGGG
GCTTTCGCCCGACCGCATGGTGGTGGTGGTCGGACCCGACATGGAGCTGGTCGGCAAGACCGTCGCCCCCTTCCCCACTG
TCATCCAGGCCGACCGGCTGGGCACCGGCCATGCCGTGTTGCAGGCCAAGGCGGCGCTGGGGCAGTTCGACGGCGACGTG
CTGGTGCTTTACGGCGACACGCCGCTGATCACCCGCGCCACGCTGGAGCGCATGCTGGCCGAGCGCCGGGGTCCCCGTGA
CCCGGCGGTGGTAGTGCTGGGCTTCAAGCCTCAGGACCCCGGCCATTACGGCCGCCTGGTGGTGGGTGCCGAAGGGCTGA
AGGCCATCGTCGAATACCGCGACGCCACCCCCGACCAGCGCGAGAATCCGCTGTGCAATTCCGGCGTCATGGCCATCGAC
GGCAATCGTCTCTGGAGCCTGATCGAGCGGGTCGACAACAAGAACTCCAAGGGTGAGTACTATCTGACCGACATCGTCGC
CCTGGCCCGCGCCGACGGCGCCACCTGCACCCATGTGGAGGGCGACGAGGCCGAGCTGCTGGGCGTCAACGCCCGATCGG
AACTGGCGGTGGCCGAAGCCCTGGTCCAGGCCCGCCTGCGCGAGGCCGCCATGGATAACGGCGCCACCCTGATCGACCCC
GCCACCGTCTGGTTCTCGTGGGACACCAAAATCGGCCGCGACGTCACCATCTGGCCGCATGTGGTGTTCGGCCCCGGCGT
CACCGTGGGCGACAACGTGGAGATCAAGGGCTTTTGCCATTTCGAGGGCTGCATGGTCGAGGCGGGCGTAGCCGCCGGCC
CCTTCACGCGGCTGCGCCCCGGCGCCGAAATCGGCGAAGGCGCCCATATCGGCAATTTCGTCGAGGTCAAGAAGGCCACG
GTCGAAGCCGGCGCCAAGATCAACCATCTGGCCTATGTGGGCGATGCCCGCGTCGGGGCCGGCGCCAATGTGGGGGCCGG
CACCATCACCTGCAATTACGACGGCTTCAACAAGTCGTTCACCGATATCGGCGCGGGGGCCTTCATCGGCTCCAACACCT
CGCTGGTCGCGCCGGTGAAGGTGGGCGACGGTGCCGTGGTCGGCGCCGGCTCGGTGATCACCAAGGAAGTGACTCCAGGC
GCGCTGGCGGTGGCGCGCGGCTCGCAGATGGAGCTGAAGGGCTGGGCCGAGCGCTTCCGCGCCCAGCAAGCCGCCAAGAA
AGCGAAGAAGGACTAG

Upstream 100 bases:

>100_bases
GGCACCTTCCGGCCACAACCGCAACAGATTGGGTCCAGCCCGCATCATGGCTTCCAGCAAGCTCGCCGTCATCGTCCTTG
CCGCCGGCATGGGCACGCGC

Downstream 100 bases:

>100_bases
ACGCCATGTGCGGCATCGTCGGAATCATCGGCAAAGTGGAAGCGGCCCCCCTCCTGGTGGAGGGATTGCGGCGCCTGGAA
TATCGCGGCTATGACTCGGC

Product: N-acetylglucosamine-1-phosphate uridyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKSSLPKVMHPLAGRPMVSHLLDTVSGLSPDRMVVVVGPDMELVGKTVAPFPTVIQADRLGTGHAVLQAKAALGQFDGDV
LVLYGDTPLITRATLERMLAERRGPRDPAVVVLGFKPQDPGHYGRLVVGAEGLKAIVEYRDATPDQRENPLCNSGVMAID
GNRLWSLIERVDNKNSKGEYYLTDIVALARADGATCTHVEGDEAELLGVNARSELAVAEALVQARLREAAMDNGATLIDP
ATVWFSWDTKIGRDVTIWPHVVFGPGVTVGDNVEIKGFCHFEGCMVEAGVAAGPFTRLRPGAEIGEGAHIGNFVEVKKAT
VEAGAKINHLAYVGDARVGAGANVGAGTITCNYDGFNKSFTDIGAGAFIGSNTSLVAPVKVGDGAVVGAGSVITKEVTPG
ALAVARGSQMELKGWAERFRAQQAAKKAKKD

Sequences:

>Translated_431_residues
MKSSLPKVMHPLAGRPMVSHLLDTVSGLSPDRMVVVVGPDMELVGKTVAPFPTVIQADRLGTGHAVLQAKAALGQFDGDV
LVLYGDTPLITRATLERMLAERRGPRDPAVVVLGFKPQDPGHYGRLVVGAEGLKAIVEYRDATPDQRENPLCNSGVMAID
GNRLWSLIERVDNKNSKGEYYLTDIVALARADGATCTHVEGDEAELLGVNARSELAVAEALVQARLREAAMDNGATLIDP
ATVWFSWDTKIGRDVTIWPHVVFGPGVTVGDNVEIKGFCHFEGCMVEAGVAAGPFTRLRPGAEIGEGAHIGNFVEVKKAT
VEAGAKINHLAYVGDARVGAGANVGAGTITCNYDGFNKSFTDIGAGAFIGSNTSLVAPVKVGDGAVVGAGSVITKEVTPG
ALAVARGSQMELKGWAERFRAQQAAKKAKKD
>Mature_431_residues
MKSSLPKVMHPLAGRPMVSHLLDTVSGLSPDRMVVVVGPDMELVGKTVAPFPTVIQADRLGTGHAVLQAKAALGQFDGDV
LVLYGDTPLITRATLERMLAERRGPRDPAVVVLGFKPQDPGHYGRLVVGAEGLKAIVEYRDATPDQRENPLCNSGVMAID
GNRLWSLIERVDNKNSKGEYYLTDIVALARADGATCTHVEGDEAELLGVNARSELAVAEALVQARLREAAMDNGATLIDP
ATVWFSWDTKIGRDVTIWPHVVFGPGVTVGDNVEIKGFCHFEGCMVEAGVAAGPFTRLRPGAEIGEGAHIGNFVEVKKAT
VEAGAKINHLAYVGDARVGAGANVGAGTITCNYDGFNKSFTDIGAGAFIGSNTSLVAPVKVGDGAVVGAGSVITKEVTPG
ALAVARGSQMELKGWAERFRAQQAAKKAKKD

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=435, Percent_Identity=40.4597701149425, Blast_Score=297, Evalue=9e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_MAGSA (Q2W7U1)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_420643.1
- ProteinModelPortal:   Q2W7U1
- SMR:   Q2W7U1
- STRING:   Q2W7U1
- GeneID:   3806004
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb1280
- NMPDR:   fig|342108.5.peg.1138
- eggNOG:   COG1207
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- PhylomeDB:   Q2W7U1
- BioCyc:   MMAG342108:AMB1280-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR001228
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF01128 IspD; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 45384; Mature: 45384

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 347-347 BINDING 74-74 BINDING 142-142 BINDING 156-156 BINDING 171-171 BINDING 371-371 BINDING 389-389 BINDING 407-407 BINDING 424-424

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSLPKVMHPLAGRPMVSHLLDTVSGLSPDRMVVVVGPDMELVGKTVAPFPTVIQADRL
CCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCCCEECCCC
GTGHAVLQAKAALGQFDGDVLVLYGDTPLITRATLERMLAERRGPRDPAVVVLGFKPQDP
CCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GHYGRLVVGAEGLKAIVEYRDATPDQRENPLCNSGVMAIDGNRLWSLIERVDNKNSKGEY
CCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCE
YLTDIVALARADGATCTHVEGDEAELLGVNARSELAVAEALVQARLREAAMDNGATLIDP
EHHHHHHHHHCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
ATVWFSWDTKIGRDVTIWPHVVFGPGVTVGDNVEIKGFCHFEGCMVEAGVAAGPFTRLRP
EEEEEEECCCCCCEEEECCEEEECCCEEECCCEEEEEEEEECCEEEECCCCCCCCHHCCC
GAEIGEGAHIGNFVEVKKATVEAGAKINHLAYVGDARVGAGANVGAGTITCNYDGFNKSF
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCH
TDIGAGAFIGSNTSLVAPVKVGDGAVVGAGSVITKEVTPGALAVARGSQMELKGWAERFR
HHCCCCEEECCCCCEEEEEEECCCCEEECCCEEEECCCCCEEEEECCCCCHHHHHHHHHH
AQQAAKKAKKD
HHHHHHHHCCC
>Mature Secondary Structure
MKSSLPKVMHPLAGRPMVSHLLDTVSGLSPDRMVVVVGPDMELVGKTVAPFPTVIQADRL
CCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCCCEECCCC
GTGHAVLQAKAALGQFDGDVLVLYGDTPLITRATLERMLAERRGPRDPAVVVLGFKPQDP
CCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GHYGRLVVGAEGLKAIVEYRDATPDQRENPLCNSGVMAIDGNRLWSLIERVDNKNSKGEY
CCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCE
YLTDIVALARADGATCTHVEGDEAELLGVNARSELAVAEALVQARLREAAMDNGATLIDP
EHHHHHHHHHCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
ATVWFSWDTKIGRDVTIWPHVVFGPGVTVGDNVEIKGFCHFEGCMVEAGVAAGPFTRLRP
EEEEEEECCCCCCEEEECCEEEECCCEEECCCEEEEEEEEECCEEEECCCCCCCCHHCCC
GAEIGEGAHIGNFVEVKKATVEAGAKINHLAYVGDARVGAGANVGAGTITCNYDGFNKSF
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCH
TDIGAGAFIGSNTSLVAPVKVGDGAVVGAGSVITKEVTPGALAVARGSQMELKGWAERFR
HHCCCCEEECCCCCEEEEEEECCCCEEECCCEEEECCCCCEEEEECCCCCHHHHHHHHHH
AQQAAKKAKKD
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA