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The map label for this gene is lysS [H]

Identifier: 83310382

GI number: 83310382

Start: 1402022

End: 1403068

Strand: Reverse

Name: lysS [H]

Synonym: amb1283

Alternate gene names: 83310382

Gene position: 1403068-1402022 (Counterclockwise)

Preceding gene: 83310390

Following gene: 83310379

Centisome position: 28.25

GC content: 69.91

Gene sequence:

>1047_bases
ATGGCAGGTAAAGAATGGTGGCGACCCGAGGTGTTCGCCCGGCGCAAGGCGGCCCTGGACCTGCGCGGCCGCGTGGTCAA
CGCCACCCGCGCCTTCTTCGCCGCCCGCGACTTCGCCGAGGTCGAGACTCCCTGTCTGCAGGTCTCGCCGGGCCTGGAGC
CCCATCTCAAGGCCTTCGCCACCGCCTTTGCCGATCCCTTCGGCGGGCCGGACCGGCCGCTTTACCTTCACACCAGCCCC
GAATTCGCCATGAAGAAGCTGCTGGTGGCGGGCGTGCCGCGCCTGTACCAGCTGGCCAAGGTTTTCCGCAATGCCGAGCG
CTCGGCCACCCATCACCCGGAATTCACCATGCTGGAATGGTACCGGGCCGGAGCCTCCATCGAGGATCTGATGGCCGACA
CCGAGGCCCTGGTCCGAGATTGCGCCAAGGCCGCCGGCGTCGGCCTGTTGCGGCGCCGGGGGACCACCTGCGACCCCTTC
GCCCCCTGGCGGCGGCTGTCGGTGGCCGAGGCCTTTGCCGAATACGCCGGCATCGACGTGCTGGCCAGTGCCCCCGACCC
TTGGTCGCCCGACCGGTCGGTCCTGGCCACCGAGGCGGCGCGCATCGGCCTCTCGGTCAGCCCGTCGGACAGTTGGGAGG
ATATCTTCTTCAAGGTGATGCTGGACCGGGTCGAGCCCCATCTCGGCTTCGACCGACCGGTCATCCTCCATTCCTACCCG
GTCTCCATGGCGGCGCTGTCGCGCCCCTCGCCCGCCGACCCCCGGGTGGCCGAGCGCTTCGAGGCCTATGCCCTGGGCGT
GGAGCTGTGCAACGCCTTCGGTGAGCTGACCGACCCCGCCGAACAGCGCCGCCGCTTCACCGCCGACATGGACCTGAAGC
AGGCCCTGTACGGCGAGCGCTATCCCCTGGACGAGGATTTCCTGACGGCCCTGGACGCGGGCATGCCGGACTCCGCCGGC
ATCGCCCTAGGCTTCGACCGGCTGGTGATGCTGCTGGCGGGGACGGAGACCATCGACGACGTGCTGTGGGCGCCGGTGGC
CGGCTAG

Upstream 100 bases:

>100_bases
ACCGCCTCGACGGACGGGATCAGCCCGGCGTCATGCAGGTCTTGGGCGGTGCGTAGGGTTCGGCGGCTCGACATGGGGTC
CCTAGATAGAGGAAAAGGCC

Downstream 100 bases:

>100_bases
ATCACATTTTCCGTCGTCATCCCGAGCGAGAGCGAGGGATCTCCGCTTGGCATGGCGGTGCCTATTCCGAGACGCCGGAC
CAGGACGAGATCCCTCCTCC

Product: hypothetical protein

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MAGKEWWRPEVFARRKAALDLRGRVVNATRAFFAARDFAEVETPCLQVSPGLEPHLKAFATAFADPFGGPDRPLYLHTSP
EFAMKKLLVAGVPRLYQLAKVFRNAERSATHHPEFTMLEWYRAGASIEDLMADTEALVRDCAKAAGVGLLRRRGTTCDPF
APWRRLSVAEAFAEYAGIDVLASAPDPWSPDRSVLATEAARIGLSVSPSDSWEDIFFKVMLDRVEPHLGFDRPVILHSYP
VSMAALSRPSPADPRVAERFEAYALGVELCNAFGELTDPAEQRRRFTADMDLKQALYGERYPLDEDFLTALDAGMPDSAG
IALGFDRLVMLLAGTETIDDVLWAPVAG

Sequences:

>Translated_348_residues
MAGKEWWRPEVFARRKAALDLRGRVVNATRAFFAARDFAEVETPCLQVSPGLEPHLKAFATAFADPFGGPDRPLYLHTSP
EFAMKKLLVAGVPRLYQLAKVFRNAERSATHHPEFTMLEWYRAGASIEDLMADTEALVRDCAKAAGVGLLRRRGTTCDPF
APWRRLSVAEAFAEYAGIDVLASAPDPWSPDRSVLATEAARIGLSVSPSDSWEDIFFKVMLDRVEPHLGFDRPVILHSYP
VSMAALSRPSPADPRVAERFEAYALGVELCNAFGELTDPAEQRRRFTADMDLKQALYGERYPLDEDFLTALDAGMPDSAG
IALGFDRLVMLLAGTETIDDVLWAPVAG
>Mature_347_residues
AGKEWWRPEVFARRKAALDLRGRVVNATRAFFAARDFAEVETPCLQVSPGLEPHLKAFATAFADPFGGPDRPLYLHTSPE
FAMKKLLVAGVPRLYQLAKVFRNAERSATHHPEFTMLEWYRAGASIEDLMADTEALVRDCAKAAGVGLLRRRGTTCDPFA
PWRRLSVAEAFAEYAGIDVLASAPDPWSPDRSVLATEAARIGLSVSPSDSWEDIFFKVMLDRVEPHLGFDRPVILHSYPV
SMAALSRPSPADPRVAERFEAYALGVELCNAFGELTDPAEQRRRFTADMDLKQALYGERYPLDEDFLTALDAGMPDSAGI
ALGFDRLVMLLAGTETIDDVLWAPVAG

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=343, Percent_Identity=29.1545189504373, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI194272210, Length=343, Percent_Identity=29.1545189504373, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=38.9570552147239, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1790571, Length=326, Percent_Identity=33.7423312883436, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1789256, Length=331, Percent_Identity=33.2326283987915, Blast_Score=177, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI71994340, Length=351, Percent_Identity=31.3390313390313, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17535925, Length=351, Percent_Identity=31.3390313390313, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17535927, Length=351, Percent_Identity=31.3390313390313, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320242, Length=336, Percent_Identity=30.0595238095238, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324256, Length=351, Percent_Identity=25.9259259259259, Blast_Score=116, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24640849, Length=347, Percent_Identity=28.8184438040346, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24640851, Length=347, Percent_Identity=28.8184438040346, Blast_Score=138, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 38447; Mature: 38316

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKEWWRPEVFARRKAALDLRGRVVNATRAFFAARDFAEVETPCLQVSPGLEPHLKAFA
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHH
TAFADPFGGPDRPLYLHTSPEFAMKKLLVAGVPRLYQLAKVFRNAERSATHHPEFTMLEW
HHHHCCCCCCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHH
YRAGASIEDLMADTEALVRDCAKAAGVGLLRRRGTTCDPFAPWRRLSVAEAFAEYAGIDV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEE
LASAPDPWSPDRSVLATEAARIGLSVSPSDSWEDIFFKVMLDRVEPHLGFDRPVILHSYP
EECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCC
VSMAALSRPSPADPRVAERFEAYALGVELCNAFGELTDPAEQRRRFTADMDLKQALYGER
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHCCCC
YPLDEDFLTALDAGMPDSAGIALGFDRLVMLLAGTETIDDVLWAPVAG
CCCCHHHHHHHHCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure 
AGKEWWRPEVFARRKAALDLRGRVVNATRAFFAARDFAEVETPCLQVSPGLEPHLKAFA
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHH
TAFADPFGGPDRPLYLHTSPEFAMKKLLVAGVPRLYQLAKVFRNAERSATHHPEFTMLEW
HHHHCCCCCCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHH
YRAGASIEDLMADTEALVRDCAKAAGVGLLRRRGTTCDPFAPWRRLSVAEAFAEYAGIDV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEE
LASAPDPWSPDRSVLATEAARIGLSVSPSDSWEDIFFKVMLDRVEPHLGFDRPVILHSYP
EECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCC
VSMAALSRPSPADPRVAERFEAYALGVELCNAFGELTDPAEQRRRFTADMDLKQALYGER
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHCCCC
YPLDEDFLTALDAGMPDSAGIALGFDRLVMLLAGTETIDDVLWAPVAG
CCCCHHHHHHHHCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA