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The map label for this gene is asnB [H]

Identifier: 83310365

GI number: 83310365

Start: 1350157

End: 1351914

Strand: Reverse

Name: asnB [H]

Synonym: amb1266

Alternate gene names: 83310365

Gene position: 1351914-1350157 (Counterclockwise)

Preceding gene: 83310366

Following gene: 83310364

Centisome position: 27.22

GC content: 69.28

Gene sequence:

>1758_bases
ATGTGCGGCATCGCCGGCGTCATGACCGCCAACGGCCCGCCCGACGGGCAGGTGCTGGACAAGCTGGCCGACGCCCTGGC
CCATCGCGGCCCCGACGGACGCGGCCGCCATGTGAGCGGCAATGTGGGGCTGGTGCAGAACCGGCTGTCCATCATCGACC
TGGAGGGCGGCAGGCAGCCGATCCTGGACGGCGCGGGCAACGCCATCGTGGCCAATGGCGAGATCTACAATTACCTGGAA
CTGAAATCGGACATGGCGGGACAGGTCTTCGCCACCGGCTCCGATTGCGAGCCGCCGCTGCACCTGTACCGCCACCAGGG
CCCGGCCTTCGCCCGCTCGCTTCGCGGCATGTACGCCTTCGCCATCCACGACGGCGGGGCGCAGAAGCTGGTTCTCGGCC
GCGATCCCTTCGGCATCAAGCCGCTTTACCTGACGGAGACCGCTCCCGGCCTGGCCTTCGCCTCCGAGCCCCAGGCCCTG
ATCGCCGCCGGGCTGGTCAAGCCCGAGCTGGACCCCGCCGCCCTGGCGGAATTGCTGCAATTGCAGTTCACCACCGGCAC
CCGGACCATCTTCAAGGGCATTCGCCGCCTGGGTGCGGGCGAGACCGTGGTGGCCGAAGGAGGCTGCGTCACGGAAGGCC
GCCGCCTGCCCGCCTTGCCCGCCGGCGGGCCGGTGGACGGCGGCCTGGACCAGATGCTGGACCAGTTGGACGACACGCTG
ACCGAATCGGTGGAACTGCACCAGCGCTCGGACGTGCCCTATGGCATGTTCCTGTCGGGCGGCATCGATTCGTCGGTGGT
GCTGGCCCTGATGGCCCGGCTCAACCCCAAGGGCGTGCTGGCCTTCACCGCCGGCTTTCCCGGCACCAGGGTGCATGACG
AGCGCCAGCATGCCCGCGATCTGGCCCGCGCCGTGGGCGCCCGCCACGTGGAGGTGGAGTTCACGGAAAGCGACTTCTGG
GAGCTGCTGCCCTTGGTCGCCGCCGCCATGGACGATCCGGCCGCCGACTACGCCTGCCTGCCCACCTTCAAGCTGGCCCG
CGAGGCGAGGAAGGAGGTCAAGGTCATCCTGTCGGGCGAAGGCGGCGACGAACTGTTCGGCGGCTATGGCCGCTATCGCC
ACGCCATGCGCCCCTGGCCGCTGACCAAGTCCATGCGCCGCAAGGGCACCTTCGACGGCCTCGACGTGCTGCGCGACGGA
ATCACCAGCCACTGGCGCCACGGCATGAAGCTGGCCGAGCGGGCCGAGGCCCTGCCCGGCCGCACCCGGCTGCAGGTGGC
CCAGGCGGTCGACTGCACCGACTGGCTGCCCAACGACCTGCTGACCAAGGCCGACCGCTGCCTGATGGCCAACGGCATCG
AGGGGCGCGTGCCCTTCCTCGATCCGGTGGTCGCCGCCTTCGCCTTCCGCCTGCCCGACCATCTCAAGGTCCGGAAGGGG
CTGGGCAAGTGGATCCTGCGCCGCTGGCTGGAGACGGCACTGCCCGCCGCCCGCCCCTTCGACAAGAAGCGCGGCTTCAC
CGTGCCGGTGGCCGAGTGGATCGCGGCGAAACCCCAAATCGGCCAATTGGTGGCCCGACAGCCCGGCATCGCCGAGATCT
GCCATCCCGGCGCGGTCGAGGCGCTGTTCAGGACTTGCGCCAAGGACACGGGCTTCGCCTGTTGGACGCTGCTGTTCTAC
GCCCTTTGGCATCAAAGACACGTCCTGGGCAAGATTCCCGCCGGAGATGCCTTCTCCGAGTTGGCGGATAGGATATAA

Upstream 100 bases:

>100_bases
GGAAGGATCTCGTCCTGATCCCATGCGTCAGAATCGGCACCAGCGGAGCAGGCGGAGATCCCTCGCCGACGCTCGGGATG
ACATCGATGGAGCGACGCTG

Downstream 100 bases:

>100_bases
TCGCTGCGTAACTTAAGTGGAGAAGCGCCATGGCCCAGTCCTTCGACCTGATCCTGCGCAACGGCATGGTGGTGACCCCC
AACGGCACCGGACGCGCCGA

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MCGIAGVMTANGPPDGQVLDKLADALAHRGPDGRGRHVSGNVGLVQNRLSIIDLEGGRQPILDGAGNAIVANGEIYNYLE
LKSDMAGQVFATGSDCEPPLHLYRHQGPAFARSLRGMYAFAIHDGGAQKLVLGRDPFGIKPLYLTETAPGLAFASEPQAL
IAAGLVKPELDPAALAELLQLQFTTGTRTIFKGIRRLGAGETVVAEGGCVTEGRRLPALPAGGPVDGGLDQMLDQLDDTL
TESVELHQRSDVPYGMFLSGGIDSSVVLALMARLNPKGVLAFTAGFPGTRVHDERQHARDLARAVGARHVEVEFTESDFW
ELLPLVAAAMDDPAADYACLPTFKLAREARKEVKVILSGEGGDELFGGYGRYRHAMRPWPLTKSMRRKGTFDGLDVLRDG
ITSHWRHGMKLAERAEALPGRTRLQVAQAVDCTDWLPNDLLTKADRCLMANGIEGRVPFLDPVVAAFAFRLPDHLKVRKG
LGKWILRRWLETALPAARPFDKKRGFTVPVAEWIAAKPQIGQLVARQPGIAEICHPGAVEALFRTCAKDTGFACWTLLFY
ALWHQRHVLGKIPAGDAFSELADRI

Sequences:

>Translated_585_residues
MCGIAGVMTANGPPDGQVLDKLADALAHRGPDGRGRHVSGNVGLVQNRLSIIDLEGGRQPILDGAGNAIVANGEIYNYLE
LKSDMAGQVFATGSDCEPPLHLYRHQGPAFARSLRGMYAFAIHDGGAQKLVLGRDPFGIKPLYLTETAPGLAFASEPQAL
IAAGLVKPELDPAALAELLQLQFTTGTRTIFKGIRRLGAGETVVAEGGCVTEGRRLPALPAGGPVDGGLDQMLDQLDDTL
TESVELHQRSDVPYGMFLSGGIDSSVVLALMARLNPKGVLAFTAGFPGTRVHDERQHARDLARAVGARHVEVEFTESDFW
ELLPLVAAAMDDPAADYACLPTFKLAREARKEVKVILSGEGGDELFGGYGRYRHAMRPWPLTKSMRRKGTFDGLDVLRDG
ITSHWRHGMKLAERAEALPGRTRLQVAQAVDCTDWLPNDLLTKADRCLMANGIEGRVPFLDPVVAAFAFRLPDHLKVRKG
LGKWILRRWLETALPAARPFDKKRGFTVPVAEWIAAKPQIGQLVARQPGIAEICHPGAVEALFRTCAKDTGFACWTLLFY
ALWHQRHVLGKIPAGDAFSELADRI
>Mature_585_residues
MCGIAGVMTANGPPDGQVLDKLADALAHRGPDGRGRHVSGNVGLVQNRLSIIDLEGGRQPILDGAGNAIVANGEIYNYLE
LKSDMAGQVFATGSDCEPPLHLYRHQGPAFARSLRGMYAFAIHDGGAQKLVLGRDPFGIKPLYLTETAPGLAFASEPQAL
IAAGLVKPELDPAALAELLQLQFTTGTRTIFKGIRRLGAGETVVAEGGCVTEGRRLPALPAGGPVDGGLDQMLDQLDDTL
TESVELHQRSDVPYGMFLSGGIDSSVVLALMARLNPKGVLAFTAGFPGTRVHDERQHARDLARAVGARHVEVEFTESDFW
ELLPLVAAAMDDPAADYACLPTFKLAREARKEVKVILSGEGGDELFGGYGRYRHAMRPWPLTKSMRRKGTFDGLDVLRDG
ITSHWRHGMKLAERAEALPGRTRLQVAQAVDCTDWLPNDLLTKADRCLMANGIEGRVPFLDPVVAAFAFRLPDHLKVRKG
LGKWILRRWLETALPAARPFDKKRGFTVPVAEWIAAKPQIGQLVARQPGIAEICHPGAVEALFRTCAKDTGFACWTLLFY
ALWHQRHVLGKIPAGDAFSELADRI

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=518, Percent_Identity=26.6409266409266, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI168229252, Length=518, Percent_Identity=26.6409266409266, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI168229250, Length=518, Percent_Identity=26.6409266409266, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=28.7003610108303, Blast_Score=186, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17560178, Length=519, Percent_Identity=26.2042389210019, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71993933, Length=513, Percent_Identity=27.8752436647173, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI25147557, Length=513, Percent_Identity=27.8752436647173, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6325403, Length=525, Percent_Identity=26.0952380952381, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321563, Length=556, Percent_Identity=25.7194244604317, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45553209, Length=554, Percent_Identity=27.7978339350181, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 63395; Mature: 63395

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGVMTANGPPDGQVLDKLADALAHRGPDGRGRHVSGNVGLVQNRLSIIDLEGGRQP
CCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEECCEEEEEECCCCCC
ILDGAGNAIVANGEIYNYLELKSDMAGQVFATGSDCEPPLHLYRHQGPAFARSLRGMYAF
CCCCCCCEEEECCCEEEHHHHHHCCCCEEEECCCCCCCCHHHHHCCCHHHHHHHCCEEEE
AIHDGGAQKLVLGRDPFGIKPLYLTETAPGLAFASEPQALIAAGLVKPELDPAALAELLQ
EEECCCCCEEEECCCCCCCCCEEEECCCCCCEECCCCHHHHHHHCCCCCCCHHHHHHHHH
LQFTTGTRTIFKGIRRLGAGETVVAEGGCVTEGRRLPALPAGGPVDGGLDQMLDQLDDTL
HHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TESVELHQRSDVPYGMFLSGGIDSSVVLALMARLNPKGVLAFTAGFPGTRVHDERQHARD
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHH
LARAVGARHVEVEFTESDFWELLPLVAAAMDDPAADYACLPTFKLAREARKEVKVILSGE
HHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEEEEECC
GGDELFGGYGRYRHAMRPWPLTKSMRRKGTFDGLDVLRDGITSHWRHGMKLAERAEALPG
CCHHHHCCHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RTRLQVAQAVDCTDWLPNDLLTKADRCLMANGIEGRVPFLDPVVAAFAFRLPDHLKVRKG
HHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
LGKWILRRWLETALPAARPFDKKRGFTVPVAEWIAAKPQIGQLVARQPGIAEICHPGAVE
HHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHCCCCHHHHHHHCCCCHHHHCCCHHHH
ALFRTCAKDTGFACWTLLFYALWHQRHVLGKIPAGDAFSELADRI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MCGIAGVMTANGPPDGQVLDKLADALAHRGPDGRGRHVSGNVGLVQNRLSIIDLEGGRQP
CCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEECCEEEEEECCCCCC
ILDGAGNAIVANGEIYNYLELKSDMAGQVFATGSDCEPPLHLYRHQGPAFARSLRGMYAF
CCCCCCCEEEECCCEEEHHHHHHCCCCEEEECCCCCCCCHHHHHCCCHHHHHHHCCEEEE
AIHDGGAQKLVLGRDPFGIKPLYLTETAPGLAFASEPQALIAAGLVKPELDPAALAELLQ
EEECCCCCEEEECCCCCCCCCEEEECCCCCCEECCCCHHHHHHHCCCCCCCHHHHHHHHH
LQFTTGTRTIFKGIRRLGAGETVVAEGGCVTEGRRLPALPAGGPVDGGLDQMLDQLDDTL
HHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TESVELHQRSDVPYGMFLSGGIDSSVVLALMARLNPKGVLAFTAGFPGTRVHDERQHARD
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHH
LARAVGARHVEVEFTESDFWELLPLVAAAMDDPAADYACLPTFKLAREARKEVKVILSGE
HHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEEEEECC
GGDELFGGYGRYRHAMRPWPLTKSMRRKGTFDGLDVLRDGITSHWRHGMKLAERAEALPG
CCHHHHCCHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RTRLQVAQAVDCTDWLPNDLLTKADRCLMANGIEGRVPFLDPVVAAFAFRLPDHLKVRKG
HHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
LGKWILRRWLETALPAARPFDKKRGFTVPVAEWIAAKPQIGQLVARQPGIAEICHPGAVE
HHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHCCCCHHHHHHHCCCCHHHHCCCHHHH
ALFRTCAKDTGFACWTLLFYALWHQRHVLGKIPAGDAFSELADRI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]