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The map label for this gene is pyrC [H]

Identifier: 83310364

GI number: 83310364

Start: 1348796

End: 1350127

Strand: Reverse

Name: pyrC [H]

Synonym: amb1265

Alternate gene names: 83310364

Gene position: 1350127-1348796 (Counterclockwise)

Preceding gene: 83310365

Following gene: 83310363

Centisome position: 27.18

GC content: 69.67

Gene sequence:

>1332_bases
ATGGCCCAGTCCTTCGACCTGATCCTGCGCAACGGCATGGTGGTGACCCCCAACGGCACCGGACGCGCCGACATCGCGGT
CAGCGACGGCCGCATCCGGGCCTTGGGCAGCCTGCTCGACGCCTCGGCCGCCGATGAGGTGGACCTGCGCGGCCTGACCG
TGCTGCCCGGAATCATCGACACCCAGGTACATTTCCGCGAGCCGGGCCTGGAGCACAAGGAAGACCTGGAAAGCGGCACG
CGCGCCGCCGCCCAGGGCGGCGTGGTCGCCATCTTCGAGATGCCCAACACCAAGCCGTCCACCACCACCGCCGACGCCAT
CCGGGACAAGCTGGACCGCGCCCAGGGCCGCGCCTGGGTGGATCACGCCTTCTTCATCGGCGCCGCCTCGGACAACATCG
ACCATCTGGCCGAGTGGGAGCGCATCCCCGGCTGCGCCGGCATCAAGGTGTTCATGGGGTCGTCCACCGGCAACCTGCTG
GTGGCCGACGACGAGACCCTGGGCCGGGTGCTGGCCCAGGGCTTCCGCCGCATCGCCGTCCATTGCGAGGACGAGGGCCG
CCTGATCGAGCGCAAGCACGTGGCCGAGGACGGCGCCCATCCCCGCGTCCATCACCAGTGGCGCGACGTGGAGACCGCCC
TGATGGCCAGCAGGCGCCTCGTCGCCCTGGCGGAAAAGGCCGGGCGCCGCGTCCACGTGCTGCACGTCACCACCGCCGAG
GAAATGGAGTTCCTGGCCGGTCACAAGGACGTGGCCACGGTGGAGACCACGCCGCAGCACCTGACCCTGGCCGCGCCGGA
ATGCTATGAGCGCCTGGGCACCTACGCCCAGATGAACCCGCCCATCCGCGGCACCCGCCATCGCGACGCCCTGTGGAAGG
CCATCGCCGACGGCGTCGTCGACGTGCTGGGCTCGGACCACGCGCCCCACACCCGCGAGGAGAAGGACAAGCCCTATCCG
CAAAGCCCGTCGGGCATGACCGGCGTCCAGACCCTGGTGCCCCTGATGCTCGACCACGTCAATCATGGCCGCCTCAGCCT
GGAACGCCTGGTGGACCTGACCAGCGCAGGCCCGGCCCGCATCTATAATATTGCCGGCAAGGGCCGCATCGCCGTGGGCT
ATGACGCCGACTTCACCGTCGTCGACATGAAGGCCGAGCGCACCATCACCAACAACTGGATCGAAAGCCGCTGCGGCTGG
ACGCCCTTCGACGGGCAGAAGGTCACCGGCTGGCCGGTGGCCACCATCGTGCGCGGCCGCACGGTCATGCGCGACGGCCA
GTTGCTAGGCCAGGCCTCGGGCGAGCCGGTCCGCTTCCAGGAAACCCTGTAG

Upstream 100 bases:

>100_bases
GGCATCAAAGACACGTCCTGGGCAAGATTCCCGCCGGAGATGCCTTCTCCGAGTTGGCGGATAGGATATAATCGCTGCGT
AACTTAAGTGGAGAAGCGCC

Downstream 100 bases:

>100_bases
GCCCGGCCGGTGGAGTTCCGCCCCTCCAACCGCCGCATCATGGTGGCGCTGCTGGGAGCCATGCTCCTGGTGCTGTGGGG
CTTCGTCACCTATTGGTCAT

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAQSFDLILRNGMVVTPNGTGRADIAVSDGRIRALGSLLDASAADEVDLRGLTVLPGIIDTQVHFREPGLEHKEDLESGT
RAAAQGGVVAIFEMPNTKPSTTTADAIRDKLDRAQGRAWVDHAFFIGAASDNIDHLAEWERIPGCAGIKVFMGSSTGNLL
VADDETLGRVLAQGFRRIAVHCEDEGRLIERKHVAEDGAHPRVHHQWRDVETALMASRRLVALAEKAGRRVHVLHVTTAE
EMEFLAGHKDVATVETTPQHLTLAAPECYERLGTYAQMNPPIRGTRHRDALWKAIADGVVDVLGSDHAPHTREEKDKPYP
QSPSGMTGVQTLVPLMLDHVNHGRLSLERLVDLTSAGPARIYNIAGKGRIAVGYDADFTVVDMKAERTITNNWIESRCGW
TPFDGQKVTGWPVATIVRGRTVMRDGQLLGQASGEPVRFQETL

Sequences:

>Translated_443_residues
MAQSFDLILRNGMVVTPNGTGRADIAVSDGRIRALGSLLDASAADEVDLRGLTVLPGIIDTQVHFREPGLEHKEDLESGT
RAAAQGGVVAIFEMPNTKPSTTTADAIRDKLDRAQGRAWVDHAFFIGAASDNIDHLAEWERIPGCAGIKVFMGSSTGNLL
VADDETLGRVLAQGFRRIAVHCEDEGRLIERKHVAEDGAHPRVHHQWRDVETALMASRRLVALAEKAGRRVHVLHVTTAE
EMEFLAGHKDVATVETTPQHLTLAAPECYERLGTYAQMNPPIRGTRHRDALWKAIADGVVDVLGSDHAPHTREEKDKPYP
QSPSGMTGVQTLVPLMLDHVNHGRLSLERLVDLTSAGPARIYNIAGKGRIAVGYDADFTVVDMKAERTITNNWIESRCGW
TPFDGQKVTGWPVATIVRGRTVMRDGQLLGQASGEPVRFQETL
>Mature_442_residues
AQSFDLILRNGMVVTPNGTGRADIAVSDGRIRALGSLLDASAADEVDLRGLTVLPGIIDTQVHFREPGLEHKEDLESGTR
AAAQGGVVAIFEMPNTKPSTTTADAIRDKLDRAQGRAWVDHAFFIGAASDNIDHLAEWERIPGCAGIKVFMGSSTGNLLV
ADDETLGRVLAQGFRRIAVHCEDEGRLIERKHVAEDGAHPRVHHQWRDVETALMASRRLVALAEKAGRRVHVLHVTTAEE
MEFLAGHKDVATVETTPQHLTLAAPECYERLGTYAQMNPPIRGTRHRDALWKAIADGVVDVLGSDHAPHTREEKDKPYPQ
SPSGMTGVQTLVPLMLDHVNHGRLSLERLVDLTSAGPARIYNIAGKGRIAVGYDADFTVVDMKAERTITNNWIESRCGWT
PFDGQKVTGWPVATIVRGRTVMRDGQLLGQASGEPVRFQETL

Specific function: Involved In The Anaerobic Utilization Of Allantoin. [C]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=414, Percent_Identity=32.1256038647343, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI19923821, Length=467, Percent_Identity=26.9807280513919, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI4503375, Length=458, Percent_Identity=26.8558951965066, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI62422571, Length=452, Percent_Identity=25.2212389380531, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI4503051, Length=448, Percent_Identity=25, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI4503377, Length=445, Percent_Identity=24.2696629213483, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI190194363, Length=450, Percent_Identity=24.8888888888889, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786722, Length=446, Percent_Identity=33.1838565022422, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI87082175, Length=458, Percent_Identity=28.82096069869, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI193204318, Length=392, Percent_Identity=31.1224489795918, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17539558, Length=461, Percent_Identity=28.8503253796095, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71989490, Length=448, Percent_Identity=28.125, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI86575075, Length=440, Percent_Identity=23.1818181818182, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322218, Length=408, Percent_Identity=28.921568627451, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24642586, Length=398, Percent_Identity=32.6633165829146, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI18859883, Length=433, Percent_Identity=28.8683602771363, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI221377917, Length=476, Percent_Identity=28.3613445378151, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137462, Length=472, Percent_Identity=27.5423728813559, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR002195
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 48399; Mature: 48268

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00483 DIHYDROOROTASE_2 ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSFDLILRNGMVVTPNGTGRADIAVSDGRIRALGSLLDASAADEVDLRGLTVLPGIID
CCCCHHHHHHCCEEECCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHC
TQVHFREPGLEHKEDLESGTRAAAQGGVVAIFEMPNTKPSTTTADAIRDKLDRAQGRAWV
CEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEE
DHAFFIGAASDNIDHLAEWERIPGCAGIKVFMGSSTGNLLVADDETLGRVLAQGFRRIAV
EEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHEEEE
HCEDEGRLIERKHVAEDGAHPRVHHQWRDVETALMASRRLVALAEKAGRRVHVLHVTTAE
EECCCCCEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHH
EMEFLAGHKDVATVETTPQHLTLAAPECYERLGTYAQMNPPIRGTRHRDALWKAIADGVV
HHHHHCCCCCEEEEECCCCEEEEECHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHH
DVLGSDHAPHTREEKDKPYPQSPSGMTGVQTLVPLMLDHVNHGRLSLERLVDLTSAGPAR
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEE
IYNIAGKGRIAVGYDADFTVVDMKAERTITNNWIESRCGWTPFDGQKVTGWPVATIVRGR
EEEECCCCEEEEECCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCEECCCCHHHHHCCC
TVMRDGQLLGQASGEPVRFQETL
EEEECCHHHHCCCCCCEEEECCC
>Mature Secondary Structure 
AQSFDLILRNGMVVTPNGTGRADIAVSDGRIRALGSLLDASAADEVDLRGLTVLPGIID
CCCHHHHHHCCEEECCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHC
TQVHFREPGLEHKEDLESGTRAAAQGGVVAIFEMPNTKPSTTTADAIRDKLDRAQGRAWV
CEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEE
DHAFFIGAASDNIDHLAEWERIPGCAGIKVFMGSSTGNLLVADDETLGRVLAQGFRRIAV
EEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHEEEE
HCEDEGRLIERKHVAEDGAHPRVHHQWRDVETALMASRRLVALAEKAGRRVHVLHVTTAE
EECCCCCEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHH
EMEFLAGHKDVATVETTPQHLTLAAPECYERLGTYAQMNPPIRGTRHRDALWKAIADGVV
HHHHHCCCCCEEEEECCCCEEEEECHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHH
DVLGSDHAPHTREEKDKPYPQSPSGMTGVQTLVPLMLDHVNHGRLSLERLVDLTSAGPAR
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEE
IYNIAGKGRIAVGYDADFTVVDMKAERTITNNWIESRCGWTPFDGQKVTGWPVATIVRGR
EEEECCCCEEEEECCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCEECCCCHHHHHCCC
TVMRDGQLLGQASGEPVRFQETL
EEEECCHHHHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA