Click here to switch to the map view.

The map label for this gene is amiD [H]

Identifier: 83309958

GI number: 83309958

Start: 918230

End: 918910

Strand: Reverse

Name: amiD [H]

Synonym: amb0859

Alternate gene names: 83309958

Gene position: 918910-918230 (Counterclockwise)

Preceding gene: 83309959

Following gene: 83309956

Centisome position: 18.5

GC content: 68.72

Gene sequence:

>681_bases
ATGACCATCATTTCCCGCCCCTCGCCCAATTTCGAGCCGCGCCCGGCCGGGGCCATCGTCGACACCCTGGTGCTGCACTA
TACCGGCATGGAATCGGGCGAGGCGGCCCTGGCCCGGCTGTGCGACGCCCAAGCCAAGGTCAGCGCCCATTACGTCATCG
AGGAAGACGGCCGCGTCTTCGCCCTGGTGCCCGAGGACATGCGCGCCTGGCACGCCGGCGCCTCGTCCTGGCGGGGCGAG
CCAGACGTCAATTCCCGCTCCATCGGCATCGAACTGGTCAATCCCGGTCACGAATTCGGCTATCGCGCGTTTCCCGGGGC
CCAGATCGAGTCGCTGATCCGCCTGTGCCGGGGAATCCTGGAGCGTCATCCCATTCCGGCCCGCAACGTGGTCGGCCATT
CCGACGTGGCGCCCACCCGCAAGGAAGACCCGGGCGAGTTGTTCCCTTGGGCCGACCTGGCCGAGCGCCACGGTATCGGC
CTGTGGCCGTGCGGCGAGCCCACGCCGCTGCCGCCCGAGCACGTGCTGCTGTCGGGCCTGGCCCATGTGGGTTACGACAT
CCACGATCCCAAGGCGACGCTGACCGCCTTCCAGCGCCATTTCCGGCCCTGGAAGGTGGACGGGCTGGTCGACGAGGAAA
GCGTCGGCCGCCTGCGCTCGCTGCTCAGGATCGTGCGTTAG

Upstream 100 bases:

>100_bases
ACAAGCTGGTGGGCGGCGCCCTGACCGTGCTGGGCGTCGCCGTGGTGGAACTGCTGCCCGGCCGCGCCATCCACCGTGCG
GAAGAACCGGAGCCCGGAAC

Downstream 100 bases:

>100_bases
GCGCCCTTCTGGATCTTGAAGGTATAGACGCCGGCCTCCTGCTCCTGGCTGAGCAGGGTGTTACCGGTCTGGCGGCAGAA
GGCGTCGAAATCGGCGACCG

Product: negative regulator of beta-lactamase expression

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTIISRPSPNFEPRPAGAIVDTLVLHYTGMESGEAALARLCDAQAKVSAHYVIEEDGRVFALVPEDMRAWHAGASSWRGE
PDVNSRSIGIELVNPGHEFGYRAFPGAQIESLIRLCRGILERHPIPARNVVGHSDVAPTRKEDPGELFPWADLAERHGIG
LWPCGEPTPLPPEHVLLSGLAHVGYDIHDPKATLTAFQRHFRPWKVDGLVDEESVGRLRSLLRIVR

Sequences:

>Translated_226_residues
MTIISRPSPNFEPRPAGAIVDTLVLHYTGMESGEAALARLCDAQAKVSAHYVIEEDGRVFALVPEDMRAWHAGASSWRGE
PDVNSRSIGIELVNPGHEFGYRAFPGAQIESLIRLCRGILERHPIPARNVVGHSDVAPTRKEDPGELFPWADLAERHGIG
LWPCGEPTPLPPEHVLLSGLAHVGYDIHDPKATLTAFQRHFRPWKVDGLVDEESVGRLRSLLRIVR
>Mature_225_residues
TIISRPSPNFEPRPAGAIVDTLVLHYTGMESGEAALARLCDAQAKVSAHYVIEEDGRVFALVPEDMRAWHAGASSWRGEP
DVNSRSIGIELVNPGHEFGYRAFPGAQIESLIRLCRGILERHPIPARNVVGHSDVAPTRKEDPGELFPWADLAERHGIGL
WPCGEPTPLPPEHVLLSGLAHVGYDIHDPKATLTAFQRHFRPWKVDGLVDEESVGRLRSLLRIVR

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=232, Percent_Identity=39.2241379310345, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786300, Length=162, Percent_Identity=37.037037037037, Blast_Score=97, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 24975; Mature: 24843

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIISRPSPNFEPRPAGAIVDTLVLHYTGMESGEAALARLCDAQAKVSAHYVIEEDGRVF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCEE
ALVPEDMRAWHAGASSWRGEPDVNSRSIGIELVNPGHEFGYRAFPGAQIESLIRLCRGIL
EECCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHHHHH
ERHPIPARNVVGHSDVAPTRKEDPGELFPWADLAERHGIGLWPCGEPTPLPPEHVLLSGL
HHCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
AHVGYDIHDPKATLTAFQRHFRPWKVDGLVDEESVGRLRSLLRIVR
HHHCCCCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TIISRPSPNFEPRPAGAIVDTLVLHYTGMESGEAALARLCDAQAKVSAHYVIEEDGRVF
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCEE
ALVPEDMRAWHAGASSWRGEPDVNSRSIGIELVNPGHEFGYRAFPGAQIESLIRLCRGIL
EECCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHHHHH
ERHPIPARNVVGHSDVAPTRKEDPGELFPWADLAERHGIGLWPCGEPTPLPPEHVLLSGL
HHCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
AHVGYDIHDPKATLTAFQRHFRPWKVDGLVDEESVGRLRSLLRIVR
HHHCCCCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]