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The map label for this gene is rpoD [H]

Identifier: 83309796

GI number: 83309796

Start: 751269

End: 753308

Strand: Reverse

Name: rpoD [H]

Synonym: amb0697

Alternate gene names: 83309796

Gene position: 753308-751269 (Counterclockwise)

Preceding gene: 83309797

Following gene: 83309793

Centisome position: 15.17

GC content: 65.15

Gene sequence:

>2040_bases
ATGGCGACCAAATCGACGAATACGGCCGAAGTTTCCGAAAACCAGGATGAGGCGATGGATGGCCCGCTGCTCGACACCCT
GGGTGTCGCCGTCAAGAAGATGGTGGCCAAGGGCAAGGAGCGCGGCTACGTCACCTATGACGAGTTGAACGCCGCCATGC
CGCCCGAGGAGGTGTCGTCCGAGCAGATCGAAGACACCATGGCCATGCTGTCCGAACTGGGCATCAACGTGGTCGAGAGC
GAGGAAGGCGAGGACAACACCCCCGAAGCCGCCGAGGAAGAAACCACCGAGACCGAGGGCTATTCCGGCGCCGGCAACGT
GGACGAGGAAGACCTGGGCCGCACCGACGACCCGGTCCGCATGTATCTGCGCGAGATGGGCTCGGTGGAACTGCTGTCGC
GCGAGGGCGAAATCGCCATCGCCAAGCGCATCGAAGCCGGCCGCGAGATGATGATCGGCGGCATCTGCGAAAGCCCCCTG
ACGCTGCGCGCCGTGGTCGACTGGCATGACGCCCTGGTCGAGGGCAAGATGCTGCTGCGCGACATCATCGACCTGGACGC
CACCTATGGCGCCGACCCCGAGCCCGAGGAACTGGCCGAGGTGGAAGCCGAGGGCGTCATCGTCGAGGAGGCCGCCCCCG
CCGGCGCCGCCGAAGCCAAGCCGGAAGGCGAGGCCGAGCCCAAGGCCGAGGGCGAGGAAGGTGAGAAGGAAGAGGGCGCC
GAGGGCGAGCCCGAGGTCGAGGGCGAAGGCGAGGCCGAGGAAGGCGAGGAGAACTCCATCTCGCTGGCCGCCATGGAAAC
CGAGCTGCTGCCCATCGTGCTCGAAACCTTCGAGGTCATCGCCGCCACTCACCACAAGCTGGAAAAGGTCCAGGACCAGC
GCCTGGCCGCCCTGAACAAGGGCGAGGCGGTCACCCCCGCCATGGAGAAGAAGTACGACAAGCTGAAGCTCGAGCTTGTC
CGCCTGATGGATGGCATCCACCTCAACAACGCCCGCATCGAGCAGCTGGTCGAGCAGCTGAACGGCCTCAACCGCCGCCT
GATGATGCAGGAAGGCCGTCTGCTGCGCCTGGCCGAAAGCTGCCGGGTCAAGCGCCAGGACTTCCTCGACGCCTATTACG
GCTCGGAGCTGGACCCAAACTGGATCGAGCAGGTCACCCAGCGCTCCAAGCAGTGGAAGGATTTCGGCGCCCGTCACGGC
AAGGACGCCGCCGACATCCGCGCCACCATCGCCGACATCTCGGCCGAAGCCGGCCTGCCCATCAGCGAATACCGCCGCAT
CGTCCAGACGGTGCAGCGCGGCGAGCGCGAGGCCAGCCGCGCCAAGAAGGAGATGATCGAGGCCAATCTGCGTCTGGTGA
TCTCCATCGCCAAGAAGTACACCAATCGCGGCCTGCAATTCCTCGACCTGATCCAGGAGGGCAATATCGGCCTGATGAAG
GCGGTGGATAAGTTCGAATACCGCCGCGGCTACAAGTTCTCCACCTATGCCACCTGGTGGATCCGTCAGGCCATCACCCG
CTCCATCGCCGATCAGGCCCGCACCATCCGCATTCCGGTGCACATGATCGAGACCATCAACAAGCTGGTCCGCACCTCGC
GCCAGATGCTGCACGAGATCGGCCGCGAGCCGACCCCGGAAGAGCTGGCGGAAAAGCTGTCCATGCCGCTGGAGAAGGTC
CGCAAGGTCCTGAAGATCGCCAAGGAGCCCATCTCGCTGGAAACCCCCATCGGCGACGAGGAAGACAGCCACCTGGGCGA
CTTCATCGAGGACAAGAACGCCGTCCTGCCGTTGGACGCCGCCATCCAGGCCAATCTGCGCGAGACCACCACCCGCGTGC
TGGCCAGCCTCACGCCCCGCGAGGAGCGCGTGCTGCGCATGCGCTTCGGCATCGGCATGAACACCGACCACACCCTGGAA
GAAGTCGGCCAGCAGTTCAGCGTCACCCGCGAACGTATCCGCCAGATCGAGGCCAAGGCGCTCCGCAAGCTCAAGCACCC
GTCGCGCTCGCGCAAGCTGCGCTCGTTCCTGGATACCTGA

Upstream 100 bases:

>100_bases
AGCGTCCGGGAACGGCTGACGACTTTGATTTCAGAGAGGGGCGAGATCGGGCGGGTCCGATGTCGCTCGGGGTCAAGACC
CTGAAGGAGCAGCATTTTTC

Downstream 100 bases:

>100_bases
TTTATCCAGCCTGCCAGCCCCCCTCCCCTCAACGGGAGGGGGACCGTCGCTGGCGCGATTTCCCGGGCTCCCGCCCGGCC
CCGGTTGGAGGCTCGCCTCC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A; Major vegetative sigma factor [H]

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MATKSTNTAEVSENQDEAMDGPLLDTLGVAVKKMVAKGKERGYVTYDELNAAMPPEEVSSEQIEDTMAMLSELGINVVES
EEGEDNTPEAAEEETTETEGYSGAGNVDEEDLGRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREMMIGGICESPL
TLRAVVDWHDALVEGKMLLRDIIDLDATYGADPEPEELAEVEAEGVIVEEAAPAGAAEAKPEGEAEPKAEGEEGEKEEGA
EGEPEVEGEGEAEEGEENSISLAAMETELLPIVLETFEVIAATHHKLEKVQDQRLAALNKGEAVTPAMEKKYDKLKLELV
RLMDGIHLNNARIEQLVEQLNGLNRRLMMQEGRLLRLAESCRVKRQDFLDAYYGSELDPNWIEQVTQRSKQWKDFGARHG
KDAADIRATIADISAEAGLPISEYRRIVQTVQRGEREASRAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMK
AVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTSRQMLHEIGREPTPEELAEKLSMPLEKV
RKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAVLPLDAAIQANLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLE
EVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDT

Sequences:

>Translated_679_residues
MATKSTNTAEVSENQDEAMDGPLLDTLGVAVKKMVAKGKERGYVTYDELNAAMPPEEVSSEQIEDTMAMLSELGINVVES
EEGEDNTPEAAEEETTETEGYSGAGNVDEEDLGRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREMMIGGICESPL
TLRAVVDWHDALVEGKMLLRDIIDLDATYGADPEPEELAEVEAEGVIVEEAAPAGAAEAKPEGEAEPKAEGEEGEKEEGA
EGEPEVEGEGEAEEGEENSISLAAMETELLPIVLETFEVIAATHHKLEKVQDQRLAALNKGEAVTPAMEKKYDKLKLELV
RLMDGIHLNNARIEQLVEQLNGLNRRLMMQEGRLLRLAESCRVKRQDFLDAYYGSELDPNWIEQVTQRSKQWKDFGARHG
KDAADIRATIADISAEAGLPISEYRRIVQTVQRGEREASRAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMK
AVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTSRQMLHEIGREPTPEELAEKLSMPLEKV
RKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAVLPLDAAIQANLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLE
EVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDT
>Mature_678_residues
ATKSTNTAEVSENQDEAMDGPLLDTLGVAVKKMVAKGKERGYVTYDELNAAMPPEEVSSEQIEDTMAMLSELGINVVESE
EGEDNTPEAAEEETTETEGYSGAGNVDEEDLGRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREMMIGGICESPLT
LRAVVDWHDALVEGKMLLRDIIDLDATYGADPEPEELAEVEAEGVIVEEAAPAGAAEAKPEGEAEPKAEGEEGEKEEGAE
GEPEVEGEGEAEEGEENSISLAAMETELLPIVLETFEVIAATHHKLEKVQDQRLAALNKGEAVTPAMEKKYDKLKLELVR
LMDGIHLNNARIEQLVEQLNGLNRRLMMQEGRLLRLAESCRVKRQDFLDAYYGSELDPNWIEQVTQRSKQWKDFGARHGK
DAADIRATIADISAEAGLPISEYRRIVQTVQRGEREASRAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKA
VDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTSRQMLHEIGREPTPEELAEKLSMPLEKVR
KVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAVLPLDAAIQANLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEE
VGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=363, Percent_Identity=62.8099173553719, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1789098, Length=238, Percent_Identity=42.0168067226891, Blast_Score=215, Evalue=1e-56,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=27.6859504132231, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.2178217821782, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 76087; Mature: 75956

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKSTNTAEVSENQDEAMDGPLLDTLGVAVKKMVAKGKERGYVTYDELNAAMPPEEVSS
CCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHCCCCCCHHHHHH
EQIEDTMAMLSELGINVVESEEGEDNTPEAAEEETTETEGYSGAGNVDEEDLGRTDDPVR
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHCCCCCHHHH
MYLREMGSVELLSREGEIAIAKRIEAGREMMIGGICESPLTLRAVVDWHDALVEGKMLLR
HHHHHCCCCEECCCCCCCHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DIIDLDATYGADPEPEELAEVEAEGVIVEEAAPAGAAEAKPEGEAEPKAEGEEGEKEEGA
HHHHHCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EGEPEVEGEGEAEEGEENSISLAAMETELLPIVLETFEVIAATHHKLEKVQDQRLAALNK
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEAVTPAMEKKYDKLKLELVRLMDGIHLNNARIEQLVEQLNGLNRRLMMQEGRLLRLAES
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHH
CRVKRQDFLDAYYGSELDPNWIEQVTQRSKQWKDFGARHGKDAADIRATIADISAEAGLP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
ISEYRRIVQTVQRGEREASRAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH
AVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTSRQMLHEI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHH
GREPTPEELAEKLSMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAVLPLDA
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEECCHH
AIQANLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRKLKHPSRSRKLRSFLDT
HHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
ATKSTNTAEVSENQDEAMDGPLLDTLGVAVKKMVAKGKERGYVTYDELNAAMPPEEVSS
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHCCCCCCHHHHHH
EQIEDTMAMLSELGINVVESEEGEDNTPEAAEEETTETEGYSGAGNVDEEDLGRTDDPVR
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHCCCCCHHHH
MYLREMGSVELLSREGEIAIAKRIEAGREMMIGGICESPLTLRAVVDWHDALVEGKMLLR
HHHHHCCCCEECCCCCCCHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DIIDLDATYGADPEPEELAEVEAEGVIVEEAAPAGAAEAKPEGEAEPKAEGEEGEKEEGA
HHHHHCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EGEPEVEGEGEAEEGEENSISLAAMETELLPIVLETFEVIAATHHKLEKVQDQRLAALNK
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEAVTPAMEKKYDKLKLELVRLMDGIHLNNARIEQLVEQLNGLNRRLMMQEGRLLRLAES
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHH
CRVKRQDFLDAYYGSELDPNWIEQVTQRSKQWKDFGARHGKDAADIRATIADISAEAGLP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
ISEYRRIVQTVQRGEREASRAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH
AVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTSRQMLHEI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHH
GREPTPEELAEKLSMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAVLPLDA
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEECCHH
AIQANLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRKLKHPSRSRKLRSFLDT
HHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491721; 11743193; 11743194 [H]