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The map label for this gene is dnaG [H]

Identifier: 83309797

GI number: 83309797

Start: 753390

End: 755261

Strand: Reverse

Name: dnaG [H]

Synonym: amb0698

Alternate gene names: 83309797

Gene position: 755261-753390 (Counterclockwise)

Preceding gene: 83309798

Following gene: 83309796

Centisome position: 15.21

GC content: 67.84

Gene sequence:

>1872_bases
ATGGCCTTCCCTCCGCAATTCCTCGACGAGCTGCGCGCCCGCATTCCGGTCACCTCGGTGGTGGCGCGGCGCGTCAAGCT
GGTGCGCAAGGGGCGCGAGCATCAGGGCCTGTGCCCCTTCCATAACGAGAAGACCCCGTCGTTCACCGTCAACGAGGACA
AGGGGTTCTTTCACTGCTTTGGCTGCGGCGCCCATGGTGATGCCATCGGCTTCGAGATGAGGGCCGGGCACCTGTCCTTC
ACCGAGGCGGTGGAGAAGCTGGCGGCCGAGGCCGGCATAGAGGTTCCCAAGGCCAGCCCAGAGGAGCGGGTGCGCGAGCA
GCGCCGCGCCTCGCTGCATGACGCCCTCGAAGCCGCCTCGGCTTTCTTCGAGAAGCATCTGCGCGGCCCGGCGGGACGCG
ACGGCTTCGCCTATCTCAAGAACCGGGGGCTGTCCGACGACACCATCGCCCGCTTCCGCCTAGGGTTCGCCCCCGACTCG
CGCGAAGCCCTCAAATCGGCGCTGATGAGCAAGGAATGCCCGGAAAGCCTGCTGATCGAGGCGGGCCTGTTGAAAAAGCC
GGAAAGCGGGGCGTCCTTCGACTATTTCCGCGGCCGGGTGATGTTTCCCATCACCGACCGGCGTGGCCGGGTGATCGGCT
TTGGCGCCCGCACCCTGGGCGACGGCCAGCCCAAATACCTTAACTCGCCCGACACGCCGCTGTTCCACAAGGGCCAGACC
CTCTACGGCCTGGCTCATGCCCGCGAGATGGCCCGCGAGTTGAACCGGGTGGTGGTGGTCGAGGGCTATATGGACGTCAT
CGCCCTGCATCAGGCGGGCTTCGCCTACGCGGTGGCCCCCCTGGGCACGGCGGTGACCGAGCAGCAGATCGAGGAGCTGT
GGCGCCTCGCCCCCGAGCCCATCCTCTGCCTGGACGGCGACAAGGCGGGACAGCGGGCCATGGAGCGGGCACTGGAGCGG
GCCTTCCCCATCCTCAAGCCCGGCCAGTCCCTGCGCTTCGCCACCCTGCCCGCCCCCGAGGACCCGGATTCGCTGATCAA
GGCCCGTGGCCCCGCCGCCATGGCCGCCGTGCTGGACGCCGCCCAGCCCCTGGTCGAGGTGGCCTGGCGCGTGGCGACCC
AGGACCGGCCGCTGGACACCCCCGAGCGCCGCGCCCTGCTGGAGAAGGAACTGTCCGACAAGGCCTTTTCCATCGCCGAC
GAGACGGTGCGCTACCAGTACCTCGCCACCTTCCGCCAGAAGATCAAGGAGTCGTTCCGCCCGCCACCGCGCCCCTGGAC
CAATGCCGGCGGCGGCAAGATGGGCGGCAAGGGCTTCGGCCGCTTCCGCCCCGATCCCCAGGCGCCGCTGAAAGGTCCCG
ACGGCATGCTGCCCGGCGTCGGCCGCCCCCGCCCGCCGCGCCCCAATGCCGGCGCCCAGGAATGGATGATGCTGACCCTG
GTTCTCGACCATCCCCGCCTGATGGATTCCATCGGTGAGAGGCTGGGCGAGACCACTTTTGCGGACAAGACCCTTGACAA
TCTTCGGCGCGGGATTCTAAAGCACCTTGGGGCTGCCCGCGATCTTGACGCGGACGGCCTCGCGACCTATTTGAGAACGG
ACGGATATTCCGAGATTCTGGACTCTCTTCTCGACGCCAACGAAAGGCGGGTACGCGCACTGCGCGACGACCTTTCCACC
GAGGAGGCCCGGATTCACTGGGAGCACGTCTACAATCTTTACACCAGGAAGGACCTGCTGGCTGACGTGAGCGAAGCCAA
CGCCGAACTCGCCCAAGGCCCCACCGCCGCGTCGTGGAGCCGCTTCGAGGCGGTAAAGAAGCAGCAGCAGACGGCCAGCG
CCCTGGACGACGAAGCGTCCGGGAACGGCTGA

Upstream 100 bases:

>100_bases
ATATGACGCATGTCGCGACCTGCGGCCGCTGAGGCCGCGGCCAAGGGTGCTCAGGCACCCGCCCGGCGAGGGACAAACAT
AAACCGGATTCTAAGCCTCC

Downstream 100 bases:

>100_bases
CGACTTTGATTTCAGAGAGGGGCGAGATCGGGCGGGTCCGATGTCGCTCGGGGTCAAGACCCTGAAGGAGCAGCATTTTT
CATGGCGACCAAATCGACGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MAFPPQFLDELRARIPVTSVVARRVKLVRKGREHQGLCPFHNEKTPSFTVNEDKGFFHCFGCGAHGDAIGFEMRAGHLSF
TEAVEKLAAEAGIEVPKASPEERVREQRRASLHDALEAASAFFEKHLRGPAGRDGFAYLKNRGLSDDTIARFRLGFAPDS
REALKSALMSKECPESLLIEAGLLKKPESGASFDYFRGRVMFPITDRRGRVIGFGARTLGDGQPKYLNSPDTPLFHKGQT
LYGLAHAREMARELNRVVVVEGYMDVIALHQAGFAYAVAPLGTAVTEQQIEELWRLAPEPILCLDGDKAGQRAMERALER
AFPILKPGQSLRFATLPAPEDPDSLIKARGPAAMAAVLDAAQPLVEVAWRVATQDRPLDTPERRALLEKELSDKAFSIAD
ETVRYQYLATFRQKIKESFRPPPRPWTNAGGGKMGGKGFGRFRPDPQAPLKGPDGMLPGVGRPRPPRPNAGAQEWMMLTL
VLDHPRLMDSIGERLGETTFADKTLDNLRRGILKHLGAARDLDADGLATYLRTDGYSEILDSLLDANERRVRALRDDLST
EEARIHWEHVYNLYTRKDLLADVSEANAELAQGPTAASWSRFEAVKKQQQTASALDDEASGNG

Sequences:

>Translated_623_residues
MAFPPQFLDELRARIPVTSVVARRVKLVRKGREHQGLCPFHNEKTPSFTVNEDKGFFHCFGCGAHGDAIGFEMRAGHLSF
TEAVEKLAAEAGIEVPKASPEERVREQRRASLHDALEAASAFFEKHLRGPAGRDGFAYLKNRGLSDDTIARFRLGFAPDS
REALKSALMSKECPESLLIEAGLLKKPESGASFDYFRGRVMFPITDRRGRVIGFGARTLGDGQPKYLNSPDTPLFHKGQT
LYGLAHAREMARELNRVVVVEGYMDVIALHQAGFAYAVAPLGTAVTEQQIEELWRLAPEPILCLDGDKAGQRAMERALER
AFPILKPGQSLRFATLPAPEDPDSLIKARGPAAMAAVLDAAQPLVEVAWRVATQDRPLDTPERRALLEKELSDKAFSIAD
ETVRYQYLATFRQKIKESFRPPPRPWTNAGGGKMGGKGFGRFRPDPQAPLKGPDGMLPGVGRPRPPRPNAGAQEWMMLTL
VLDHPRLMDSIGERLGETTFADKTLDNLRRGILKHLGAARDLDADGLATYLRTDGYSEILDSLLDANERRVRALRDDLST
EEARIHWEHVYNLYTRKDLLADVSEANAELAQGPTAASWSRFEAVKKQQQTASALDDEASGNG
>Mature_622_residues
AFPPQFLDELRARIPVTSVVARRVKLVRKGREHQGLCPFHNEKTPSFTVNEDKGFFHCFGCGAHGDAIGFEMRAGHLSFT
EAVEKLAAEAGIEVPKASPEERVREQRRASLHDALEAASAFFEKHLRGPAGRDGFAYLKNRGLSDDTIARFRLGFAPDSR
EALKSALMSKECPESLLIEAGLLKKPESGASFDYFRGRVMFPITDRRGRVIGFGARTLGDGQPKYLNSPDTPLFHKGQTL
YGLAHAREMARELNRVVVVEGYMDVIALHQAGFAYAVAPLGTAVTEQQIEELWRLAPEPILCLDGDKAGQRAMERALERA
FPILKPGQSLRFATLPAPEDPDSLIKARGPAAMAAVLDAAQPLVEVAWRVATQDRPLDTPERRALLEKELSDKAFSIADE
TVRYQYLATFRQKIKESFRPPPRPWTNAGGGKMGGKGFGRFRPDPQAPLKGPDGMLPGVGRPRPPRPNAGAQEWMMLTLV
LDHPRLMDSIGERLGETTFADKTLDNLRRGILKHLGAARDLDADGLATYLRTDGYSEILDSLLDANERRVRALRDDLSTE
EARIHWEHVYNLYTRKDLLADVSEANAELAQGPTAASWSRFEAVKKQQQTASALDDEASGNG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=522, Percent_Identity=39.6551724137931, Blast_Score=348, Evalue=6e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68879; Mature: 68748

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFPPQFLDELRARIPVTSVVARRVKLVRKGREHQGLCPFHNEKTPSFTVNEDKGFFHCF
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEE
GCGAHGDAIGFEMRAGHLSFTEAVEKLAAEAGIEVPKASPEERVREQRRASLHDALEAAS
ECCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFFEKHLRGPAGRDGFAYLKNRGLSDDTIARFRLGFAPDSREALKSALMSKECPESLLIE
HHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHCCCHHHHHH
AGLLKKPESGASFDYFRGRVMFPITDRRGRVIGFGARTLGDGQPKYLNSPDTPLFHKGQT
HHCCCCCCCCCCCHHHCCEEEEEEECCCCCEEEECCHHCCCCCCCCCCCCCCCCEECCCH
LYGLAHAREMARELNRVVVVEGYMDVIALHQAGFAYAVAPLGTAVTEQQIEELWRLAPEP
HHHHHHHHHHHHHHCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
ILCLDGDKAGQRAMERALERAFPILKPGQSLRFATLPAPEDPDSLIKARGPAAMAAVLDA
EEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHCCCHHHHHHHHHH
AQPLVEVAWRVATQDRPLDTPERRALLEKELSDKAFSIADETVRYQYLATFRQKIKESFR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPPRPWTNAGGGKMGGKGFGRFRPDPQAPLKGPDGMLPGVGRPRPPRPNAGAQEWMMLTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEHHH
VLDHPRLMDSIGERLGETTFADKTLDNLRRGILKHLGAARDLDADGLATYLRTDGYSEIL
HHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHH
DSLLDANERRVRALRDDLSTEEARIHWEHVYNLYTRKDLLADVSEANAELAQGPTAASWS
HHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RFEAVKKQQQTASALDDEASGNG
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AFPPQFLDELRARIPVTSVVARRVKLVRKGREHQGLCPFHNEKTPSFTVNEDKGFFHCF
CCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEE
GCGAHGDAIGFEMRAGHLSFTEAVEKLAAEAGIEVPKASPEERVREQRRASLHDALEAAS
ECCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFFEKHLRGPAGRDGFAYLKNRGLSDDTIARFRLGFAPDSREALKSALMSKECPESLLIE
HHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHCCCHHHHHH
AGLLKKPESGASFDYFRGRVMFPITDRRGRVIGFGARTLGDGQPKYLNSPDTPLFHKGQT
HHCCCCCCCCCCCHHHCCEEEEEEECCCCCEEEECCHHCCCCCCCCCCCCCCCCEECCCH
LYGLAHAREMARELNRVVVVEGYMDVIALHQAGFAYAVAPLGTAVTEQQIEELWRLAPEP
HHHHHHHHHHHHHHCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
ILCLDGDKAGQRAMERALERAFPILKPGQSLRFATLPAPEDPDSLIKARGPAAMAAVLDA
EEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHCCCHHHHHHHHHH
AQPLVEVAWRVATQDRPLDTPERRALLEKELSDKAFSIADETVRYQYLATFRQKIKESFR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPPRPWTNAGGGKMGGKGFGRFRPDPQAPLKGPDGMLPGVGRPRPPRPNAGAQEWMMLTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEHHH
VLDHPRLMDSIGERLGETTFADKTLDNLRRGILKHLGAARDLDADGLATYLRTDGYSEIL
HHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHH
DSLLDANERRVRALRDDLSTEEARIHWEHVYNLYTRKDLLADVSEANAELAQGPTAASWS
HHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RFEAVKKQQQTASALDDEASGNG
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]