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The map label for this gene is priA

Identifier: 82750817

GI number: 82750817

Start: 1160891

End: 1163299

Strand: Direct

Name: priA

Synonym: SAB1076

Alternate gene names: 82750817

Gene position: 1160891-1163299 (Clockwise)

Preceding gene: 82750816

Following gene: 82750819

Centisome position: 42.33

GC content: 34.83

Gene sequence:

>2409_bases
ATGATAGCGAAAGTCATAGTCGATGTCGCGTCGAAGAGCGTTGACTATAAATTTGATTATATAATTCCCGAACAACTCGA
ATCTGTCATCCAACCTGGTGTGCGTGTGATTGTACCTTTTGGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAG
CAGAACCTGATGCACAACTTGACGTTTCGAAGTTAAAAAAAATCATAGAAGTGAAAGATATACAACCTGAATTAACATCA
GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATGTCATTAAACGTATTTCTATGCTAGAAGTGATGCTTCCGAG
TGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATGAAAGATGACATAGAGGTACCTTCAGCTTTATTACAAAAATTTG
ATAAGCATGGTTACTATTATTATAAAGATGCGCAAAAAAATAATGATATTCAATTGCTTATGAAGTTGTTAAAAGATGAT
ATCGTTGAAGAAAAAACGATTCTCACACAAAATATAACTAAAAAAACCAAGCGTGCTGTTCGTGTCATTGAAGGGTATCA
TCCTGATGAAGTATTAGCTAAGTTGGAGAAAGTTATTAAACAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATA
AAACAATATTTTTAACTGATATTGAGGATATGGGCTTTTCAAAATCTAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
GAAAAATATGACGCGGTTGTTGAAAGAGACCCATTTAAAGATCGTGTTTTCGAACAAGAATCAAAACAGCAATTAACAGA
AGACCAATATAAAGCATATGAAGCGATTAAAGCTAAAATTGTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGA
CGGGATCAGGTAAAACAGAAGTATATTTACAAACGATAGAAGATGTTTTAAGTCAAGGAAAACAGGCGATGATGTTAGTT
CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGCTTCAAACGTCGATTTGGTGATGACGTTGCTGTATTACATTCAGG
CTTATCTAGTGGGGAACGTTATGATGAGTGGCAAAAAATTAGGGATGGTCGTGCGAGAGTAAATGTTGGTGCAAGGTCAA
GTGTGTTCGCACCTTTCAAAAATTTAGGGTTAATCATCATTGATGAAGAACATGAATCTACATATAAGCAAGAAGATTAT
CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGCCCAGTCATTTTAGGAAGTGCAACACC
ATGTCTTGAAAGTTATGCACGAGCTGAAAAAGGCGTTTATCATTTGCTATCATTACCAAACAGAGTGAACCAACAAGCTT
TACCTGAAATTGATATAGTAGACATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
GCCATACAATTAAGATTAGATCGACAGGAACAAGTTGTTTTATTTTTAAATCGACGTGGTTATGCATCATTTATGTTATG
TCGGGATTGTGGATATGTACCGCAATGCCCAAACTGTGATATTTCATTAACGTATCATAAAACGACAGACTTATTAAAAT
GTCACTATTGTGGTTACCAAGAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACT
GGTACTCAGAAAGTTGAAGAACTATTGCAACAAGAATTTGAAGATGCGCGCATAATTAGGATGGATGTAGATACAACCTC
AAAGAAAGGTGCACATGAAAAGTTATTGACTGAATTCGAAAAAGGTAACGGTGATATTTTACTAGGTACTCAGATGATTG
CGAAAGGATTAGATTATCCAAACATTACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
GCGAGCGAACGTACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGTCATGAAAAGGCAGGTCAAGTCATCAT
TCAAACGTATAATCCAGACCATTATTCAATATTGGATGTTCAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGG
AATATCGTAAATTAGGAAAGTATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
ATGGAAGCATCACAGCATGTTCATAAAATTTTATTACAGCATTTAACAGAAAAAGCGCTTGTACTAGGTCCATCTCCGGC
AGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATTTTAGTGAAATATAAAAGTGAACCTGGATTATTACAAGCCA
TTCAATTTTTAGATGACTATTACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCACAGATG
ATGATGTAA

Upstream 100 bases:

>100_bases
CAATGCATTTTAAAAATAATGAAAAAGTAAATATCAAGAAAGGAAAAAAAGTAGTATTAGCTGCACAAATTTTAGATGAA
CTAGAAACTAGGTGGCAATA

Downstream 100 bases:

>100_bases
CATTACTAATTATTAGAAACAAGTTCAATATGTACGAGTATTTGAACCAGTCTTTGAATATTTACTTTAAGGGCAGAAAA
AGGGCAGAATATATAACTGT

Product: primosomal protein

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 802; Mature: 802

Protein sequence:

>802_residues
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSSGERYDEWQKIRDGRARVNVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Sequences:

>Translated_802_residues
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSSGERYDEWQKIRDGRARVNVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM
>Mature_802_residues
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSSGERYDEWQKIRDGRARVNVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=618, Percent_Identity=38.9967637540453, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 92540; Mature: 92540

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQL
CHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCCCC
DVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMK
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDDIVEEKTILTQNITKKTKRAV
CCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
HHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEHHHCCCCHHHHHHHHHHCHH
EKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAIKAKIVSQEQETFLLHGVTGSGKTE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCH
VYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFKRRFGDDVAVLHSGLSSGERYDEWQKI
HHHHHHHHHHHCCCCEEEECCCHHCCHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHH
RDGRARVNVGARSSVFAPFKNLGLIIIDEEHESTYKQEDYPRYHAREIAQWRSEYHHCPV
HCCCEEEECCCCCCCCCHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCE
ILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
EECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHCCCCHHHHHHHHHH
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQ
HHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEECCCC
ETPPNQCPNCESEHIRQVGTGTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFE
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHH
KGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHE
CCCCCEEECHHHHHCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHCCCCCC
KAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
CCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHH
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDY
HHHHHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHH
YHEKFIKEKLALKIDIDPQMMM
HHHHHHHHHHEEEEECCCCCCC
>Mature Secondary Structure
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQL
CHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCCCC
DVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMK
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDDIVEEKTILTQNITKKTKRAV
CCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
HHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEHHHCCCCHHHHHHHHHHCHH
EKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAIKAKIVSQEQETFLLHGVTGSGKTE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCH
VYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFKRRFGDDVAVLHSGLSSGERYDEWQKI
HHHHHHHHHHHCCCCEEEECCCHHCCHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHH
RDGRARVNVGARSSVFAPFKNLGLIIIDEEHESTYKQEDYPRYHAREIAQWRSEYHHCPV
HCCCEEEECCCCCCCCCHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCE
ILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
EECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHCCCCHHHHHHHHHH
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQ
HHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEECCCC
ETPPNQCPNCESEHIRQVGTGTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFE
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHH
KGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHE
CCCCCEEECHHHHHCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHCCCCCC
KAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
CCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHH
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDY
HHHHHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHH
YHEKFIKEKLALKIDIDPQMMM
HHHHHHHHHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]