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The map label for this gene is def

Identifier: 82750819

GI number: 82750819

Start: 1165416

End: 1165904

Strand: Direct

Name: def

Synonym: SAB1079

Alternate gene names: 82750819

Gene position: 1165416-1165904 (Clockwise)

Preceding gene: 82750817

Following gene: 82750820

Centisome position: 42.49

GC content: 33.74

Gene sequence:

>489_bases
ATGGCGATTAAAAAGTTAGTACCAGCATCGCATCCTATTTTAACGAAAAAAGCGCAAGCAGTTATAAAATTTGATGATTC
GTTAAAAAGATTATTACAAGATTTAGAAGATACAATGTATGCACAAGAAGCTGCTGGCTTATGTGCACCTCAAATTAATC
AGTCATTGCAAGTGGCAATCATTGATATGGAAATGGAAGGATTATTACAACTTGTTAATCCGAAAATTATTAGACAATCA
AATGAAACAATAACAGACTTAGAAGGTTCAATTACATTGCCAGATGTTTACGGCGAAGTGACAAGAAGTAAAATGATCGT
TGTCGAAAGTTATGACGTCAATGGGAACAAAGTTGAACTAACTGCACATGAAGATGTAGCAAGAATGATTTTGCATATTA
TAGATCAAATGAACGGTATCCCTTTTACAGAACGTGCGGACCGTATTTTAACAGATAAAGAAGTGGAGGCATATTTTATA
AATGACTAA

Upstream 100 bases:

>100_bases
AATCTTTAAATTGAAAAATATACATTCTATGATGTAAGGTCGCATTTTTAATATATTTACGTTATAATGGTTTGATGAAT
TTTATTGAGGAGTAAGAGCT

Downstream 100 bases:

>100_bases
AATAATATTTATGGGTACACCAGACTTTTCAACAACTGTTTTAGAAATGCTTATTGCAGAACATGATGTCATTGCAGTCG
TAACGCAACCAGATCGACCT

Product: peptide deformylase

Products: Formate; L-Methionine. [C]

Alternate protein names: Polypeptide deformylase-like [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEAAGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIIRQS
NETITDLEGSITLPDVYGEVTRSKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
ND

Sequences:

>Translated_162_residues
MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEAAGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIIRQS
NETITDLEGSITLPDVYGEVTRSKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
ND
>Mature_161_residues
AIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEAAGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIIRQSN
ETITDLEGSITLPDVYGEVTRSKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFIN
D

Specific function: Removes The Formyl Group From The N-Terminal Met Of Newly Synthesized Proteins. Requires At Least A Dipeptide For An Efficient Rate Of Reaction. N-Terminal L-Methionine Is A Prerequisite For Activity But The Enzyme Has Broad Specificity At Other Positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=131, Percent_Identity=30.5343511450382, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: 3.5.1.88 [C]

Molecular weight: Translated: 18183; Mature: 18052

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEAAGLCAPQINQSLQVAI
CCCCCCCCCCCCCHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
IDMEMEGLLQLVNPKIIRQSNETITDLEGSITLPDVYGEVTRSKMIVVESYDVNGNKVEL
EEECHHHHHHHHCHHHHHCCCCEEECCCCCEECCHHHHHHCCCEEEEEEEECCCCCEEEE
TAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFIND
EEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
AIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEAAGLCAPQINQSLQVAI
CCCCCCCCCCCCHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
IDMEMEGLLQLVNPKIIRQSNETITDLEGSITLPDVYGEVTRSKMIVVESYDVNGNKVEL
EEECHHHHHHHHCHHHHHCCCCEEECCCCCEECCHHHHHHCCCEEEEEEEECCCCCEEEE
TAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFIND
EEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Formyl-L-Methionine; H2O [C]

Specific reaction: N-Formyl-L-Methionine + H2O = Formate + L-Methionine. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; EDTA; o-Phenantroline [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA