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The map label for this gene is murE [H]

Identifier: 82703614

GI number: 82703614

Start: 2855487

End: 2857061

Strand: Reverse

Name: murE [H]

Synonym: Nmul_A2499

Alternate gene names: 82703614

Gene position: 2857061-2855487 (Counterclockwise)

Preceding gene: 82703615

Following gene: 82703613

Centisome position: 89.72

GC content: 55.87

Gene sequence:

>1575_bases
ATGAGTTCCAGGTCGGACAGGAAGATATCGGAATGTCCCGGCCGCCCGGGAGCCGGTTTTGATTTCCATGCGCTTGACCG
GCTCGGTGTCAGGATGGAGCATCTTGCGGTTGACAGCCGCACCGTGAAACCGGGGGATACTTTTCTGGCCTATCGCGGAG
AAAAAACGGATGGACGTAAATTCATTCCGCACGCGATAGAGCGGGGTGCCCGCGCAGTAGTGTGGGAACGCGATGGCTTT
GCATGGGACCCGGAATGGAAAACACCTAACCTGCCAGTCCCGGATTTGAAATCCGAGGCGGGATTCATCGCAGACTATTT
GTATGATCATCCCTCACAAAAGCTATGGCTGATCGGAGTGACGGGAACGAATGGCAAAACATCCTGCAGCCACTGGATTG
TCCAGGCGATGGCGGCACTCGATCGTAAAGCCGCAGTCATCGGCACGCTGGGCGCCGGTTTTCCCGGAGAAATGGAATTT
TCTGCAAATACCACCCCCGATTCAGTGTTATTGCAAGCTAAAATAGCTAATTTTTTGCGGCGGGGCGCGGAATGCGTGGC
AATGGAAGTCTCGTCGCATGGGATTGTGCAAGGACGGATAAACGGAGCAACGTTTGCAGTTGCCATGCTCACCAATCTTT
CGCGCGATCACCTCGATTATCACGGTACCATGGAAGTGTATGCAGCTGCCAAAGCGCGGCTGTTTCACTGGAGGGGGCTC
AAGTATGCCGTGCTGAACCTCGACGACGTATTTGGCATGCAGCTTTTGCACGATCTGGAAGGAACTGGAACCGGCATGAT
CGGCTATGGGTTTACCGATCCGGTAAGCGCGCACCCCAATCCTGGCTCCTTCAGGAGGGTATGGGGTCGCAATCTCAGGG
CAAGCATGGCGGGGCTGGAATTCGACATTGAATTTGAAGGGGAATGTCTGAAATTCAAAGCGGAAGTGCTGGGCAGCTTC
AACGCTTCCAACTTGTTAGGAGTGCTCGCTACCCTGCTTGCAAGCGGTATCGCTTTCCCGGAAGCGGTCCGAGCCTTGCA
GAAAGTCCAGCCTGTAGCAGGTCGCATGCAGCAGATAGGTGGGGAAGGAGATGAACCCCTCATCGTTGTGGATTACGCTC
ATACTCCGGACGCGCTGGAAAAGGTATTGACCACGCTGCGGGAAGCGGTGGACGCCGGTTTCCCTGGCGGGAAGCCGGAG
ACGGGATCAGGAAGGCTCGTCTGCGTATTCGGTTGCGGGGGCGAGCGCGATCAAGGCAAGCGTCCATTGATGGGGGAAGT
GGCGACCCGATTGGCTGATGAAGCCATCATCACCAGTGACAATCCACGAAGCGAGGATCCGGATTCCATTATTCGTGGCA
TCGCCGTTGGAGCTGCCGCCAATCATCGCATCGAGAAGGATCGGGCAGCTGCGATTCATCGCGCCATACGGGGCACGCAT
AAGGGTGATGTCGTATTGATCGCGGGTAAGGGGCACGAAACCTGGCAGGAGGCAGGTGGTAAAAAGTTTCCTTTCAGCGA
TGCAGAGGTGGCGAGAAAAGCATTGATCACGGAGAAAGCGCAGGCGCAGATATGA

Upstream 100 bases:

>100_bases
TGCACGGCATCCTGCATATGCAGAACGTGCCACACGATGCGCCGGGGGGCAATATGACCTCCCTGACAGCTGCCCCGCCC
AAGGACAACAAGGGTGATGT

Downstream 100 bases:

>100_bases
TGAGTTTGCAGGAGGCGGCCCAGGTTCTGTCCGCAACCTTGGCGGGGGGGCGCCATGATCGGATGGTTGCGGGCAAGATC
GATTTCACGGGGGTGAGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSSRSDRKISECPGRPGAGFDFHALDRLGVRMEHLAVDSRTVKPGDTFLAYRGEKTDGRKFIPHAIERGARAVVWERDGF
AWDPEWKTPNLPVPDLKSEAGFIADYLYDHPSQKLWLIGVTGTNGKTSCSHWIVQAMAALDRKAAVIGTLGAGFPGEMEF
SANTTPDSVLLQAKIANFLRRGAECVAMEVSSHGIVQGRINGATFAVAMLTNLSRDHLDYHGTMEVYAAAKARLFHWRGL
KYAVLNLDDVFGMQLLHDLEGTGTGMIGYGFTDPVSAHPNPGSFRRVWGRNLRASMAGLEFDIEFEGECLKFKAEVLGSF
NASNLLGVLATLLASGIAFPEAVRALQKVQPVAGRMQQIGGEGDEPLIVVDYAHTPDALEKVLTTLREAVDAGFPGGKPE
TGSGRLVCVFGCGGERDQGKRPLMGEVATRLADEAIITSDNPRSEDPDSIIRGIAVGAAANHRIEKDRAAAIHRAIRGTH
KGDVVLIAGKGHETWQEAGGKKFPFSDAEVARKALITEKAQAQI

Sequences:

>Translated_524_residues
MSSRSDRKISECPGRPGAGFDFHALDRLGVRMEHLAVDSRTVKPGDTFLAYRGEKTDGRKFIPHAIERGARAVVWERDGF
AWDPEWKTPNLPVPDLKSEAGFIADYLYDHPSQKLWLIGVTGTNGKTSCSHWIVQAMAALDRKAAVIGTLGAGFPGEMEF
SANTTPDSVLLQAKIANFLRRGAECVAMEVSSHGIVQGRINGATFAVAMLTNLSRDHLDYHGTMEVYAAAKARLFHWRGL
KYAVLNLDDVFGMQLLHDLEGTGTGMIGYGFTDPVSAHPNPGSFRRVWGRNLRASMAGLEFDIEFEGECLKFKAEVLGSF
NASNLLGVLATLLASGIAFPEAVRALQKVQPVAGRMQQIGGEGDEPLIVVDYAHTPDALEKVLTTLREAVDAGFPGGKPE
TGSGRLVCVFGCGGERDQGKRPLMGEVATRLADEAIITSDNPRSEDPDSIIRGIAVGAAANHRIEKDRAAAIHRAIRGTH
KGDVVLIAGKGHETWQEAGGKKFPFSDAEVARKALITEKAQAQI
>Mature_523_residues
SSRSDRKISECPGRPGAGFDFHALDRLGVRMEHLAVDSRTVKPGDTFLAYRGEKTDGRKFIPHAIERGARAVVWERDGFA
WDPEWKTPNLPVPDLKSEAGFIADYLYDHPSQKLWLIGVTGTNGKTSCSHWIVQAMAALDRKAAVIGTLGAGFPGEMEFS
ANTTPDSVLLQAKIANFLRRGAECVAMEVSSHGIVQGRINGATFAVAMLTNLSRDHLDYHGTMEVYAAAKARLFHWRGLK
YAVLNLDDVFGMQLLHDLEGTGTGMIGYGFTDPVSAHPNPGSFRRVWGRNLRASMAGLEFDIEFEGECLKFKAEVLGSFN
ASNLLGVLATLLASGIAFPEAVRALQKVQPVAGRMQQIGGEGDEPLIVVDYAHTPDALEKVLTTLREAVDAGFPGGKPET
GSGRLVCVFGCGGERDQGKRPLMGEVATRLADEAIITSDNPRSEDPDSIIRGIAVGAAANHRIEKDRAAAIHRAIRGTHK
GDVVLIAGKGHETWQEAGGKKFPFSDAEVARKALITEKAQAQI

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=491, Percent_Identity=39.5112016293279, Blast_Score=285, Evalue=5e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 56636; Mature: 56505

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRSDRKISECPGRPGAGFDFHALDRLGVRMEHLAVDSRTVKPGDTFLAYRGEKTDGRK
CCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCC
FIPHAIERGARAVVWERDGFAWDPEWKTPNLPVPDLKSEAGFIADYLYDHPSQKLWLIGV
CCHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEE
TGTNGKTSCSHWIVQAMAALDRKAAVIGTLGAGFPGEMEFSANTTPDSVLLQAKIANFLR
ECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
RGAECVAMEVSSHGIVQGRINGATFAVAMLTNLSRDHLDYHGTMEVYAAAKARLFHWRGL
CCCEEEEEEECCCCEEEEECCCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHEEECCC
KYAVLNLDDVFGMQLLHDLEGTGTGMIGYGFTDPVSAHPNPGSFRRVWGRNLRASMAGLE
EEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCE
FDIEFEGECLKFKAEVLGSFNASNLLGVLATLLASGIAFPEAVRALQKVQPVAGRMQQIG
EEEEECCCHHHEEHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
GEGDEPLIVVDYAHTPDALEKVLTTLREAVDAGFPGGKPETGSGRLVCVFGCGGERDQGK
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCC
RPLMGEVATRLADEAIITSDNPRSEDPDSIIRGIAVGAAANHRIEKDRAAAIHRAIRGTH
CCHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCCCC
KGDVVLIAGKGHETWQEAGGKKFPFSDAEVARKALITEKAQAQI
CCCEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSRSDRKISECPGRPGAGFDFHALDRLGVRMEHLAVDSRTVKPGDTFLAYRGEKTDGRK
CCCCCCCHHHCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCC
FIPHAIERGARAVVWERDGFAWDPEWKTPNLPVPDLKSEAGFIADYLYDHPSQKLWLIGV
CCHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEE
TGTNGKTSCSHWIVQAMAALDRKAAVIGTLGAGFPGEMEFSANTTPDSVLLQAKIANFLR
ECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
RGAECVAMEVSSHGIVQGRINGATFAVAMLTNLSRDHLDYHGTMEVYAAAKARLFHWRGL
CCCEEEEEEECCCCEEEEECCCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHEEECCC
KYAVLNLDDVFGMQLLHDLEGTGTGMIGYGFTDPVSAHPNPGSFRRVWGRNLRASMAGLE
EEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCE
FDIEFEGECLKFKAEVLGSFNASNLLGVLATLLASGIAFPEAVRALQKVQPVAGRMQQIG
EEEEECCCHHHEEHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
GEGDEPLIVVDYAHTPDALEKVLTTLREAVDAGFPGGKPETGSGRLVCVFGCGGERDQGK
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCC
RPLMGEVATRLADEAIITSDNPRSEDPDSIIRGIAVGAAANHRIEKDRAAAIHRAIRGTH
CCHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCCCC
KGDVVLIAGKGHETWQEAGGKKFPFSDAEVARKALITEKAQAQI
CCCEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]