Click here to switch to the map view.

The map label for this gene is penA [H]

Identifier: 82703615

GI number: 82703615

Start: 2857058

End: 2858821

Strand: Reverse

Name: penA [H]

Synonym: Nmul_A2500

Alternate gene names: 82703615

Gene position: 2858821-2857058 (Counterclockwise)

Preceding gene: 82703616

Following gene: 82703614

Centisome position: 89.78

GC content: 59.41

Gene sequence:

>1764_bases
ATGAAAGCCAATCAAAAGCCGCCTGTTATCCTGCCCGCCCAGCGCTCACGCCTCCTGGTGGGTTTATTGTTGCTTGGATT
CGCCGGGCTGGGGGGACGCGCTGCTTATCTGCAGGGAATGAACAACGGGTTTTTGCAGCAGAAGGGCGAATCGCGTTATG
GCCGAGTGCTCGAGATGAACGCAAACAGGGGAATGATCACCGATCGGTACGGCAAGCCCCTGGCAATAAGCACTCCCGTG
GAATCCGTATGGCTGAGTCCTCAGGATGTGGTTGCCACCCCCGAGCAGTTAAGCAGGCTCGCGGATCTGATCGATATGGA
TGCGGACGAGCTTCACAAGCGCCTGGGCGGACTGGCAGGAGCCTCCCAAGGTTCCCGGGCGGCGTCCCGACGCGATTTCG
TATACCTGAAGCGGCATCTGTCCCCAGCCGTGGCAGCCGGCGTCGTTCAACTGAAAATCCCCGGCATATTTCTCAAGCGC
GAGTATCGGCGCTACTACCCTGCGGGAGACCTGACTTCGCACGTCCTCGGGTTCACCGATCTGGACGACAGGGGGCAGGA
GGGTATCGAGCTGGCCTGGCAGGATGAGCTTGCTGGCAAGCCGGGAAGCCGCCGAGTCATCAAGGATCGCAAAGGCCATA
TCATCGAGGACGTCGAAAGCATCCGGGCACCGAAGCCGGGCCAGAACATGGCGCTGTCGATAGATAGCAAAATACAGTAC
CTCGCCTACCGTGAATTGAAGCAGGCGGTGGAAACCCACAAGGCCAAGGCCGGCGGCCTGGTGGCACTCGATGTGAAGAC
AGGCGAAGTCCTGGCTCTGGTGAATCTCCCGACCTACAACCCGAACAACCGGTCCAACAGGAATAGTGCGCAGTCGCGTA
ACAGGGCCGTCACCGATATGTTCGAGCCGGGTTCCACGCTCAAGCCGTTCACCGTAGCGGCAGCGCTGGAAGCAGGGAGA
ATCACCCCGGACACTGTATTTCAAACCGCGCCGGGCTCATTCACCATAGGTAAGGCGACCATACGCGATGCGCACAGGAC
AGAGGCCTTGACCGTCACGCAGGTGATCCAGAAATCCAGCAACGTCGGTTCAGCGAAGATCGCGCTGTCGCTTCCACCAC
AAACACTGTGGGAAATGCTGAATGGAGCAGGATTCGGAGAGCTGCCGGGCTCCGGTTTCCCGGGCGAGGTGAGCGGCAAG
CTGCGGCCTTATAAAACCTGGCGTCCCATCGAGCAGGCGACCATGTCGTATGGTCACGGTATTGCAGTGAGCCTGATGCA
GCTCGCTCGCGCTTATATGATTTTTGCCGCGGAAGGTGAAATAAAACCGGTATCGCTGCTGAAGCTCGACTCGGCTCCCG
TCAGCAAGCGCGCGATTTCACGCGATACAGCGCTGGCCGTGAGCCGCATGCTCGAGATGGTGGCGCAGCCAGGGGGTACC
GCTCCACAGGCGCAGGTCAGCGGATATCGGGTGGCGGGGAAGACTGGTACCGCGCACAAGCTGGAAGGCAGAGGCTATGC
AAAAAACCGCTATCTTTCGACCTTTGTCGGTTATGCGCCCGTTTCGAATCCAAGATATGTCATTGCAGTGATGCTGGACG
AGCCATCAGCCGGACAATATTACGGAGGTACGGTGGCCGCGCCGGTTTTCAGCCGGGTGATGCACGGCATCCTGCATATG
CAGAACGTGCCACACGATGCGCCGGGGGGCAATATGACCTCCCTGACAGCTGCCCCGCCCAAGGACAACAAGGGTGATGT
ATGA

Upstream 100 bases:

>100_bases
AGATGCGCTTGCCGGACCCCAAGCGTATCCAGACCATACCCTTGAGTCAGCCCCTGGGGGAACCTGTCGATTCGACCGAC
TCCAAGGAAGCATCGAAATC

Downstream 100 bases:

>100_bases
GTTCCAGGTCGGACAGGAAGATATCGGAATGTCCCGGCCGCCCGGGAGCCGGTTTTGATTTCCATGCGCTTGACCGGCTC
GGTGTCAGGATGGAGCATCT

Product: peptidoglycan glycosyltransferase

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKANQKPPVILPAQRSRLLVGLLLLGFAGLGGRAAYLQGMNNGFLQQKGESRYGRVLEMNANRGMITDRYGKPLAISTPV
ESVWLSPQDVVATPEQLSRLADLIDMDADELHKRLGGLAGASQGSRAASRRDFVYLKRHLSPAVAAGVVQLKIPGIFLKR
EYRRYYPAGDLTSHVLGFTDLDDRGQEGIELAWQDELAGKPGSRRVIKDRKGHIIEDVESIRAPKPGQNMALSIDSKIQY
LAYRELKQAVETHKAKAGGLVALDVKTGEVLALVNLPTYNPNNRSNRNSAQSRNRAVTDMFEPGSTLKPFTVAAALEAGR
ITPDTVFQTAPGSFTIGKATIRDAHRTEALTVTQVIQKSSNVGSAKIALSLPPQTLWEMLNGAGFGELPGSGFPGEVSGK
LRPYKTWRPIEQATMSYGHGIAVSLMQLARAYMIFAAEGEIKPVSLLKLDSAPVSKRAISRDTALAVSRMLEMVAQPGGT
APQAQVSGYRVAGKTGTAHKLEGRGYAKNRYLSTFVGYAPVSNPRYVIAVMLDEPSAGQYYGGTVAAPVFSRVMHGILHM
QNVPHDAPGGNMTSLTAAPPKDNKGDV

Sequences:

>Translated_587_residues
MKANQKPPVILPAQRSRLLVGLLLLGFAGLGGRAAYLQGMNNGFLQQKGESRYGRVLEMNANRGMITDRYGKPLAISTPV
ESVWLSPQDVVATPEQLSRLADLIDMDADELHKRLGGLAGASQGSRAASRRDFVYLKRHLSPAVAAGVVQLKIPGIFLKR
EYRRYYPAGDLTSHVLGFTDLDDRGQEGIELAWQDELAGKPGSRRVIKDRKGHIIEDVESIRAPKPGQNMALSIDSKIQY
LAYRELKQAVETHKAKAGGLVALDVKTGEVLALVNLPTYNPNNRSNRNSAQSRNRAVTDMFEPGSTLKPFTVAAALEAGR
ITPDTVFQTAPGSFTIGKATIRDAHRTEALTVTQVIQKSSNVGSAKIALSLPPQTLWEMLNGAGFGELPGSGFPGEVSGK
LRPYKTWRPIEQATMSYGHGIAVSLMQLARAYMIFAAEGEIKPVSLLKLDSAPVSKRAISRDTALAVSRMLEMVAQPGGT
APQAQVSGYRVAGKTGTAHKLEGRGYAKNRYLSTFVGYAPVSNPRYVIAVMLDEPSAGQYYGGTVAAPVFSRVMHGILHM
QNVPHDAPGGNMTSLTAAPPKDNKGDV
>Mature_587_residues
MKANQKPPVILPAQRSRLLVGLLLLGFAGLGGRAAYLQGMNNGFLQQKGESRYGRVLEMNANRGMITDRYGKPLAISTPV
ESVWLSPQDVVATPEQLSRLADLIDMDADELHKRLGGLAGASQGSRAASRRDFVYLKRHLSPAVAAGVVQLKIPGIFLKR
EYRRYYPAGDLTSHVLGFTDLDDRGQEGIELAWQDELAGKPGSRRVIKDRKGHIIEDVESIRAPKPGQNMALSIDSKIQY
LAYRELKQAVETHKAKAGGLVALDVKTGEVLALVNLPTYNPNNRSNRNSAQSRNRAVTDMFEPGSTLKPFTVAAALEAGR
ITPDTVFQTAPGSFTIGKATIRDAHRTEALTVTQVIQKSSNVGSAKIALSLPPQTLWEMLNGAGFGELPGSGFPGEVSGK
LRPYKTWRPIEQATMSYGHGIAVSLMQLARAYMIFAAEGEIKPVSLLKLDSAPVSKRAISRDTALAVSRMLEMVAQPGGT
APQAQVSGYRVAGKTGTAHKLEGRGYAKNRYLSTFVGYAPVSNPRYVIAVMLDEPSAGQYYGGTVAAPVFSRVMHGILHM
QNVPHDAPGGNMTSLTAAPPKDNKGDV

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=558, Percent_Identity=41.5770609318996, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1786854, Length=603, Percent_Identity=26.6998341625207, Blast_Score=145, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 63289; Mature: 63289

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKANQKPPVILPAQRSRLLVGLLLLGFAGLGGRAAYLQGMNNGFLQQKGESRYGRVLEMN
CCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHCCCCCCCCEEEEEC
ANRGMITDRYGKPLAISTPVESVWLSPQDVVATPEQLSRLADLIDMDADELHKRLGGLAG
CCCCEEECCCCCCEEEECCHHHHCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCC
ASQGSRAASRRDFVYLKRHLSPAVAAGVVQLKIPGIFLKREYRRYYPAGDLTSHVLGFTD
CCCCCHHHHHHHHHHHHHHCCHHHHHCEEEEEECCEEEEHHHHHHCCCCCHHHHHCCCCC
LDDRGQEGIELAWQDELAGKPGSRRVIKDRKGHIIEDVESIRAPKPGQNMALSIDSKIQY
CCCCCCCCCEEEEEHHHCCCCCCCCHHHCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHH
LAYRELKQAVETHKAKAGGLVALDVKTGEVLALVNLPTYNPNNRSNRNSAQSRNRAVTDM
HHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHCCCHHHH
FEPGSTLKPFTVAAALEAGRITPDTVFQTAPGSFTIGKATIRDAHRTEALTVTQVIQKSS
CCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCC
NVGSAKIALSLPPQTLWEMLNGAGFGELPGSGFPGEVSGKLRPYKTWRPIEQATMSYGHG
CCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCH
IAVSLMQLARAYMIFAAEGEIKPVSLLKLDSAPVSKRAISRDTALAVSRMLEMVAQPGGT
HHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
APQAQVSGYRVAGKTGTAHKLEGRGYAKNRYLSTFVGYAPVSNPRYVIAVMLDEPSAGQY
CCCCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
YGGTVAAPVFSRVMHGILHMQNVPHDAPGGNMTSLTAAPPKDNKGDV
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKANQKPPVILPAQRSRLLVGLLLLGFAGLGGRAAYLQGMNNGFLQQKGESRYGRVLEMN
CCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHCCCCCCCCEEEEEC
ANRGMITDRYGKPLAISTPVESVWLSPQDVVATPEQLSRLADLIDMDADELHKRLGGLAG
CCCCEEECCCCCCEEEECCHHHHCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCC
ASQGSRAASRRDFVYLKRHLSPAVAAGVVQLKIPGIFLKREYRRYYPAGDLTSHVLGFTD
CCCCCHHHHHHHHHHHHHHCCHHHHHCEEEEEECCEEEEHHHHHHCCCCCHHHHHCCCCC
LDDRGQEGIELAWQDELAGKPGSRRVIKDRKGHIIEDVESIRAPKPGQNMALSIDSKIQY
CCCCCCCCCEEEEEHHHCCCCCCCCHHHCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHH
LAYRELKQAVETHKAKAGGLVALDVKTGEVLALVNLPTYNPNNRSNRNSAQSRNRAVTDM
HHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHCCCHHHH
FEPGSTLKPFTVAAALEAGRITPDTVFQTAPGSFTIGKATIRDAHRTEALTVTQVIQKSS
CCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCC
NVGSAKIALSLPPQTLWEMLNGAGFGELPGSGFPGEVSGKLRPYKTWRPIEQATMSYGHG
CCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCH
IAVSLMQLARAYMIFAAEGEIKPVSLLKLDSAPVSKRAISRDTALAVSRMLEMVAQPGGT
HHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
APQAQVSGYRVAGKTGTAHKLEGRGYAKNRYLSTFVGYAPVSNPRYVIAVMLDEPSAGQY
CCCCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
YGGTVAAPVFSRVMHGILHMQNVPHDAPGGNMTSLTAAPPKDNKGDV
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]