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The map label for this gene is murF [H]

Identifier: 82703613

GI number: 82703613

Start: 2854075

End: 2855424

Strand: Reverse

Name: murF [H]

Synonym: Nmul_A2498

Alternate gene names: 82703613

Gene position: 2855424-2854075 (Counterclockwise)

Preceding gene: 82703614

Following gene: 82703612

Centisome position: 89.67

GC content: 55.11

Gene sequence:

>1350_bases
ATGGTTGCGGGCAAGATCGATTTCACGGGGGTGAGCACGGATAGCCGCGCACTTGCACGAGGGGAGCTGTTTGTTGCTTT
GGTCGGTCCCAACTTCGATGGTCATGATTTTATCGTCCAGGCACGGGACAGGGGCGCTATGGGGGCCCTGGTGCAGCAGG
ACAGGCTGACCGGGGATCAGGAATACGGGATTCCGCTGATACGGGTGCAGAATACACGGCTGGCATTAGGGCAACTGGCG
GCATACTGGCGCGCGCGCTTCACCATACCCCTAGTTGCAGTAACGGGAAGTAATGGCAAGACGACTGTAAAGGAGATGAT
TGCCTCCATTTTAAGGCGCGCCGCGGAGCAGGCGCCGGAGGAGATGCGAACGGATGCTCCGGATGCAGTGCTGGCAACCC
AGGGCAATCTCAACAACGATATAGGGATGCCCCTCACACTTTTGCGATTACGTGAGCGGCATCACTATGCGGTCATAGAG
ATGGGCATGAACCATCCAGGCGAAATCTCATACCTTTCCCGCCTTGCAAAGCCTTCGGTAGCGCTCATCACCAATGCCGG
CGATGCGCACATTCAGGGACTTGGGTCAGTTGAAGCAGTCGCTCGCGCAAAGGGCGAGATCTTTGAAGGACTCGACCAGC
AGGGAATAGCGGTTATCAATGCAGATGATCCACACAATAGACTGTGGCGTGAACTTGCGGGTAACAGGCAAATAATCGAT
TTTGGTCTGAAGAGTAAGGCAAAAGTAAGTGCAGACTACAAACTGACTTTTTCCGGCGCGCAAATAACACTGATTTTACC
TCAAGGGGTCGAGGAGACGACTCTGCAGGTACCGGGCGAACATAACGTGCGAAATGCGCTGGCTGCCGCGGCCGTGGCCG
TAGCCGTCGGTGTGTCCTTGAAGACGATCGCATCGGGATTGAGCACATTTGGCGGAGTGAAAGGAAGAATGCAAAGGAAA
TGCGGTGTGCGCGGCGCGACGTTGATCGATGATTGTTACAACGCCAACCCCGATTCGGTACGGGCGGCCCTGGCGGTGCT
TGCAAGGGCGGAAGGTAAAAAAGTCCTGGTGCTGGGGGACATGGGAGAACTGGGCGACAAAGGAAGAGAGTTTCATGAAC
AGATCGGTGACGAAGCGAGAATTGCGGGGATAGACGAACTCATGGCTTTGGGAGATTTGAGTGCATACGCGGCGGAAAAG
TTCGGCATCGGAGCTCGCCATTTCAGAAAAATGGAGGATCTGCTGGCAAATATAGAAACGTTGCCAGGCCCGGATGTCAC
CCTACTGATCAAAGGGTCGCGTTTCATGCAGATGGAGCGAGTCGTAAAGCGTCTGGAAGCCGGGTTATGA

Upstream 100 bases:

>100_bases
AAGCATTGATCACGGAGAAAGCGCAGGCGCAGATATGATGAGTTTGCAGGAGGCGGCCCAGGTTCTGTCCGCAACCTTGG
CGGGGGGGCGCCATGATCGG

Downstream 100 bases:

>100_bases
GTTTACAGGCCGCGAATCCAGTTATCGACGTCATAGTCCTCTTCGGTGAGTCTGGTAAATCCCGGGGCATTTCACACGGA
TGGCTGCCATGCTGCTAGAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MVAGKIDFTGVSTDSRALARGELFVALVGPNFDGHDFIVQARDRGAMGALVQQDRLTGDQEYGIPLIRVQNTRLALGQLA
AYWRARFTIPLVAVTGSNGKTTVKEMIASILRRAAEQAPEEMRTDAPDAVLATQGNLNNDIGMPLTLLRLRERHHYAVIE
MGMNHPGEISYLSRLAKPSVALITNAGDAHIQGLGSVEAVARAKGEIFEGLDQQGIAVINADDPHNRLWRELAGNRQIID
FGLKSKAKVSADYKLTFSGAQITLILPQGVEETTLQVPGEHNVRNALAAAAVAVAVGVSLKTIASGLSTFGGVKGRMQRK
CGVRGATLIDDCYNANPDSVRAALAVLARAEGKKVLVLGDMGELGDKGREFHEQIGDEARIAGIDELMALGDLSAYAAEK
FGIGARHFRKMEDLLANIETLPGPDVTLLIKGSRFMQMERVVKRLEAGL

Sequences:

>Translated_449_residues
MVAGKIDFTGVSTDSRALARGELFVALVGPNFDGHDFIVQARDRGAMGALVQQDRLTGDQEYGIPLIRVQNTRLALGQLA
AYWRARFTIPLVAVTGSNGKTTVKEMIASILRRAAEQAPEEMRTDAPDAVLATQGNLNNDIGMPLTLLRLRERHHYAVIE
MGMNHPGEISYLSRLAKPSVALITNAGDAHIQGLGSVEAVARAKGEIFEGLDQQGIAVINADDPHNRLWRELAGNRQIID
FGLKSKAKVSADYKLTFSGAQITLILPQGVEETTLQVPGEHNVRNALAAAAVAVAVGVSLKTIASGLSTFGGVKGRMQRK
CGVRGATLIDDCYNANPDSVRAALAVLARAEGKKVLVLGDMGELGDKGREFHEQIGDEARIAGIDELMALGDLSAYAAEK
FGIGARHFRKMEDLLANIETLPGPDVTLLIKGSRFMQMERVVKRLEAGL
>Mature_449_residues
MVAGKIDFTGVSTDSRALARGELFVALVGPNFDGHDFIVQARDRGAMGALVQQDRLTGDQEYGIPLIRVQNTRLALGQLA
AYWRARFTIPLVAVTGSNGKTTVKEMIASILRRAAEQAPEEMRTDAPDAVLATQGNLNNDIGMPLTLLRLRERHHYAVIE
MGMNHPGEISYLSRLAKPSVALITNAGDAHIQGLGSVEAVARAKGEIFEGLDQQGIAVINADDPHNRLWRELAGNRQIID
FGLKSKAKVSADYKLTFSGAQITLILPQGVEETTLQVPGEHNVRNALAAAAVAVAVGVSLKTIASGLSTFGGVKGRMQRK
CGVRGATLIDDCYNANPDSVRAALAVLARAEGKKVLVLGDMGELGDKGREFHEQIGDEARIAGIDELMALGDLSAYAAEK
FGIGARHFRKMEDLLANIETLPGPDVTLLIKGSRFMQMERVVKRLEAGL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=445, Percent_Identity=42.0224719101124, Blast_Score=295, Evalue=6e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48247; Mature: 48247

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAGKIDFTGVSTDSRALARGELFVALVGPNFDGHDFIVQARDRGAMGALVQQDRLTGDQ
CCCCCEEEECCCCCCHHEECCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCH
EYGIPLIRVQNTRLALGQLAAYWRARFTIPLVAVTGSNGKTTVKEMIASILRRAAEQAPE
HHCCEEEEEECCHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCH
EMRTDAPDAVLATQGNLNNDIGMPLTLLRLRERHHYAVIEMGMNHPGEISYLSRLAKPSV
HHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCE
ALITNAGDAHIQGLGSVEAVARAKGEIFEGLDQQGIAVINADDPHNRLWRELAGNRQIID
EEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCEEEE
FGLKSKAKVSADYKLTFSGAQITLILPQGVEETTLQVPGEHNVRNALAAAAVAVAVGVSL
ECCCCCCEECCCEEEEECCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCHH
KTIASGLSTFGGVKGRMQRKCGVRGATLIDDCYNANPDSVRAALAVLARAEGKKVLVLGD
HHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEC
MGELGDKGREFHEQIGDEARIAGIDELMALGDLSAYAAEKFGIGARHFRKMEDLLANIET
CHHCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
LPGPDVTLLIKGSRFMQMERVVKRLEAGL
CCCCCEEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVAGKIDFTGVSTDSRALARGELFVALVGPNFDGHDFIVQARDRGAMGALVQQDRLTGDQ
CCCCCEEEECCCCCCHHEECCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCH
EYGIPLIRVQNTRLALGQLAAYWRARFTIPLVAVTGSNGKTTVKEMIASILRRAAEQAPE
HHCCEEEEEECCHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCH
EMRTDAPDAVLATQGNLNNDIGMPLTLLRLRERHHYAVIEMGMNHPGEISYLSRLAKPSV
HHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCE
ALITNAGDAHIQGLGSVEAVARAKGEIFEGLDQQGIAVINADDPHNRLWRELAGNRQIID
EEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCEEEE
FGLKSKAKVSADYKLTFSGAQITLILPQGVEETTLQVPGEHNVRNALAAAAVAVAVGVSL
ECCCCCCEECCCEEEEECCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCHH
KTIASGLSTFGGVKGRMQRKCGVRGATLIDDCYNANPDSVRAALAVLARAEGKKVLVLGD
HHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEC
MGELGDKGREFHEQIGDEARIAGIDELMALGDLSAYAAEKFGIGARHFRKMEDLLANIET
CHHCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
LPGPDVTLLIKGSRFMQMERVVKRLEAGL
CCCCCEEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]