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The map label for this gene is cca

Identifier: 82703566

GI number: 82703566

Start: 2801689

End: 2802930

Strand: Direct

Name: cca

Synonym: Nmul_A2451

Alternate gene names: 82703566

Gene position: 2801689-2802930 (Clockwise)

Preceding gene: 82703565

Following gene: 82703577

Centisome position: 87.99

GC content: 58.94

Gene sequence:

>1242_bases
ATGAAAACCTACCTGGTGGGCGGCTCCGTGCGTGACGAGATACTGGGGCTGCCCGTAACGGATCACGATTATGTGGTTGT
CGGCGTCTCGCCGGAAGAAATGGTACATCTGGGCTTCCGCCCGGTAGGAAAAGATTTTCCCGTTTTTCTTCACCCTCAAA
GCCAGGAACAATACGCCCTGGCCCGCACGGAGCGCAAGGTTTCGCGCGGCTACAAAGGGTTTGAGGTTTATGCTTCCCCC
GAAGTTACGCTACAGGAGGACCTGGCGCGCCGTGATCTGACGATAAACGCAATCGCCAAGGATGAGTACGGCAACATCAT
CGACCCTTTCGGCGGGATCGCTGATCTGGAAGCCGGCGTGCTCCGCCATATCGGCCCGGCATTCACTGAAGACCCGGTGC
GTGTCCTGCGTACCGCTCGCTTTGCCGCACGTTTCGGTTTCCATATCGCGCCCGAAACACTAGCGCTGATGAACGAAATG
GTCCATAACGGGGAGGTGGACGCCCTGGTGTCGGAGCGGGTATGGCAGGAGATTGCACGCGGTTTGATGGAGCGCCACCC
TTCACGCATGTTTTACGCGCTGCGCGACTGTGGTGCCCTCACCCGTATCATGCCTGAAGTGGATGCCTTGTTCGGCGTGC
CGCAACCTCCGCAGCATCACCCCGAAATCGATACCGGGGTACACGTGATGATGGTGATCGATTATGCCGCTTCGAGGAAT
TATTCCCTGCAGGTCCGGTTTGCCGCGCTCACCCACGATCTCGGTAAAGGGACGACTCCTCCGGAAGAATGGCCGCGCCA
TATTGGACACGAAGCGCGCAGCGTCAGACTGGTGCAGGGCTTATGCGAACGTATCAACCCTCCCAACGAAATGCGGAACC
TGGCATTGCTGGTGGCCCGCTATCACGGAGACGTGCACCGCGCGGCCGAACTGCGACCTGTCACCATCGCCAATCTGCTT
CAGGGAGTGGATGCCTATCGCAAACCCGAGCGCTTCGAAGAATTTCTGCAAGCCTGCGCGTGTGATTTTCATGGACGGCC
GGGCTACGCAACACGGCCTTACCCGCAGGCGGATCGACTCCGCGAAGCGTTTCAGGCGGCAAGAAGCGTGGATGCGGGAG
CGATCGCCAAAGAGATGGCAAGAAACGTTTCCGATCCCAGCCGCCTGCCCGTCGCCATTAACACTCGGGTAAGCGAAACC
CGTATCGCTGAAATCAGAAACCGTCTTGGAAGCCTCGCCTGA

Upstream 100 bases:

>100_bases
CAGCACCGATATATCTGAAACATCAGTTGCCCCGTGAACGTTACAACTATCTGCGGCATTGGTCCGGGCGTTGAGATAGA
TTCATACAGATAATTCCGGA

Downstream 100 bases:

>100_bases
ATGCCGCTTTCCCCTTCGACGGCCTGAGGACTAGAATTTGCGCCTTGGCAAAGCCGGCAGAGGCTTTCTGATGGCTAGTC
AGCGCTTACATATGGCAGTG

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKTYLVGGSVRDEILGLPVTDHDYVVVGVSPEEMVHLGFRPVGKDFPVFLHPQSQEQYALARTERKVSRGYKGFEVYASP
EVTLQEDLARRDLTINAIAKDEYGNIIDPFGGIADLEAGVLRHIGPAFTEDPVRVLRTARFAARFGFHIAPETLALMNEM
VHNGEVDALVSERVWQEIARGLMERHPSRMFYALRDCGALTRIMPEVDALFGVPQPPQHHPEIDTGVHVMMVIDYAASRN
YSLQVRFAALTHDLGKGTTPPEEWPRHIGHEARSVRLVQGLCERINPPNEMRNLALLVARYHGDVHRAAELRPVTIANLL
QGVDAYRKPERFEEFLQACACDFHGRPGYATRPYPQADRLREAFQAARSVDAGAIAKEMARNVSDPSRLPVAINTRVSET
RIAEIRNRLGSLA

Sequences:

>Translated_413_residues
MKTYLVGGSVRDEILGLPVTDHDYVVVGVSPEEMVHLGFRPVGKDFPVFLHPQSQEQYALARTERKVSRGYKGFEVYASP
EVTLQEDLARRDLTINAIAKDEYGNIIDPFGGIADLEAGVLRHIGPAFTEDPVRVLRTARFAARFGFHIAPETLALMNEM
VHNGEVDALVSERVWQEIARGLMERHPSRMFYALRDCGALTRIMPEVDALFGVPQPPQHHPEIDTGVHVMMVIDYAASRN
YSLQVRFAALTHDLGKGTTPPEEWPRHIGHEARSVRLVQGLCERINPPNEMRNLALLVARYHGDVHRAAELRPVTIANLL
QGVDAYRKPERFEEFLQACACDFHGRPGYATRPYPQADRLREAFQAARSVDAGAIAKEMARNVSDPSRLPVAINTRVSET
RIAEIRNRLGSLA
>Mature_413_residues
MKTYLVGGSVRDEILGLPVTDHDYVVVGVSPEEMVHLGFRPVGKDFPVFLHPQSQEQYALARTERKVSRGYKGFEVYASP
EVTLQEDLARRDLTINAIAKDEYGNIIDPFGGIADLEAGVLRHIGPAFTEDPVRVLRTARFAARFGFHIAPETLALMNEM
VHNGEVDALVSERVWQEIARGLMERHPSRMFYALRDCGALTRIMPEVDALFGVPQPPQHHPEIDTGVHVMMVIDYAASRN
YSLQVRFAALTHDLGKGTTPPEEWPRHIGHEARSVRLVQGLCERINPPNEMRNLALLVARYHGDVHRAAELRPVTIANLL
QGVDAYRKPERFEEFLQACACDFHGRPGYATRPYPQADRLREAFQAARSVDAGAIAKEMARNVSDPSRLPVAINTRVSET
RIAEIRNRLGSLA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=386, Percent_Identity=55.440414507772, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI87081691, Length=168, Percent_Identity=36.3095238095238, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=207, Percent_Identity=32.8502415458937, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_NITMU (Q2Y681)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413132.1
- ProteinModelPortal:   Q2Y681
- SMR:   Q2Y681
- STRING:   Q2Y681
- GeneID:   3786408
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2451
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   DRIWREW
- PhylomeDB:   Q2Y681
- ProtClustDB:   PRK10885
- BioCyc:   NMUL323848:NMUL_A2451-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46250; Mature: 46250

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLVGGSVRDEILGLPVTDHDYVVVGVSPEEMVHLGFRPVGKDFPVFLHPQSQEQYAL
CCEEEECCCHHHHHCCCCCCCCCEEEEECCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKVSRGYKGFEVYASPEVTLQEDLARRDLTINAIAKDEYGNIIDPFGGIADLEAGV
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LRHIGPAFTEDPVRVLRTARFAARFGFHIAPETLALMNEMVHNGEVDALVSERVWQEIAR
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GLMERHPSRMFYALRDCGALTRIMPEVDALFGVPQPPQHHPEIDTGVHVMMVIDYAASRN
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHCCCC
YSLQVRFAALTHDLGKGTTPPEEWPRHIGHEARSVRLVQGLCERINPPNEMRNLALLVAR
CEEEEEEHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YHGDVHRAAELRPVTIANLLQGVDAYRKPERFEEFLQACACDFHGRPGYATRPYPQADRL
HHCCHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
REAFQAARSVDAGAIAKEMARNVSDPSRLPVAINTRVSETRIAEIRNRLGSLA
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTYLVGGSVRDEILGLPVTDHDYVVVGVSPEEMVHLGFRPVGKDFPVFLHPQSQEQYAL
CCEEEECCCHHHHHCCCCCCCCCEEEEECCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKVSRGYKGFEVYASPEVTLQEDLARRDLTINAIAKDEYGNIIDPFGGIADLEAGV
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LRHIGPAFTEDPVRVLRTARFAARFGFHIAPETLALMNEMVHNGEVDALVSERVWQEIAR
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GLMERHPSRMFYALRDCGALTRIMPEVDALFGVPQPPQHHPEIDTGVHVMMVIDYAASRN
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHCCCC
YSLQVRFAALTHDLGKGTTPPEEWPRHIGHEARSVRLVQGLCERINPPNEMRNLALLVAR
CEEEEEEHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YHGDVHRAAELRPVTIANLLQGVDAYRKPERFEEFLQACACDFHGRPGYATRPYPQADRL
HHCCHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
REAFQAARSVDAGAIAKEMARNVSDPSRLPVAINTRVSETRIAEIRNRLGSLA
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA