Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 82703564

GI number: 82703564

Start: 2798779

End: 2800713

Strand: Direct

Name: slt [H]

Synonym: Nmul_A2449

Alternate gene names: 82703564

Gene position: 2798779-2800713 (Clockwise)

Preceding gene: 82703562

Following gene: 82703565

Centisome position: 87.89

GC content: 57.21

Gene sequence:

>1935_bases
ATGAAGAAACTGTTGATCGGGCTGTTTTTGGCAACAACCTCCACTGCAACGTATGCAGCCGCAAGAAACCAGGACTTCCT
GGCCCTGAAGCACGCATATCAGGCGGGCAACACTGCGCGCGTGAATATGTATGCGCAGCGGCTGCGAGGGCACGTGCTTG
AACCTTATGGAGCTTATTATCAACTGCGACCGCAACTCTGGAATCCGAAGCTGAGTCCGGACACTGTCAAGCAGTTTATC
GAGAAATATCACGATAGCCCTCTCAGCGACCGCCTGCTGGGAGAATGGCTGAAGGTCCTGGGCTGGACCGAGCGATGGGA
TCTTTTCGCAGAAAACTATCCGCGCATGGTCAACAAGGATGTCGAACTGGTCTGTTACTCTCTTCAACAGCGCCTGGCGG
GAGGTGATCCCACCGTCTATAAGGAGGCATTTCCGTTATGGTTCAACGCGCGTGAGATGCCGCACAGCTGTAATCAGATA
TTCGATGTACTGATTACCGCCGGGACGCTGACAGACGACGATATCTGGGCACGGCTGCGTCTTGCGCTCGAGTCCGGCAA
TGTCAGCGCGGCAAAACGCATCAATGAATACCTGCCCAAAAATCAGCGTCTGAACGAACGCAACCTGAATGCCGCCACAG
AAAATCCTCTCCGCTATCTGGAAAGGCATCGGCGTGAGATCAAAACCCGCTCCGATCGGGAAATCGCACTTTTCGCCATG
GTGCGCTTGCTCCGCAGCGGGCTCGATCAGGCGCATGCTTATTGGCCGCGGATACGCGACCGCTTCGGTGCGCCAGACCA
GGCGTATTTTCTGGTGCAACTTGCGGACCAGGGGGCCCGGCGGCTGGACCCGCGCGCGTTGCAGTGGTTTGGAGAAGCCG
CCAATACGCACAAGTCTGTTCCCCTCACCGATGCCCAGCTTGGTTGGAAAACCCGCACCGCCTTGCGTGCCGGGGACTGG
AATCTCGTCCTGGATACAATCGAAACCATGTCGGCGGCGGAACAGAAAGAGGGGGTCTGGCGTTACTGGAAAGCGCGGGC
GTTGAAAGCTAAAGGAAAATTAAAGGAAGCAAACGCCATTCTTGCGCCGTTGAGCAGCGAGCATCATTTTTACGGGCAAT
TGGCCGAAGAGGAGTTGGGCGTTACCATCGGCGCTCCTGCGGAATCCTTCAAGCCGACGGCAAAAGAAATTTCCGCAATG
GAGCGGACGCCTGGAATCGCGCGCGCGCTGGCCTTCTACAACCTGGATTTGCGTTCGGAGGCGGCCCAGGAATGGATTTG
GGCCGTGCGAAAATTTGATGACCATGAGCTGCTGGCGGCAGCGGAAGTCGCCCGCCGTCATGGGCACTACGACCGCGCCA
TCAATACCGCCGACAGAACCGTGCAGCGCCACGATTTCCACCTGAGGTTCCTTGCACCGCATAGAGATGCGATGCGCGAC
ATCCTGAAGCAGCAGGAACTCGACGAAGCATGGGTCTACGGCCTCATTCGCCAGGAGAGCCGCTTCGTCAGCGATGCCAA
GTCCAGCGCCGGCGCTATGGGTCTGATGCAACTGATGCCCGGTACGGCAAAGTGGGCCTCCAAACAGATGGGACTGAGAA
AGTATCGCCCAAGCCGGGTGACGGAGGTAAATACCAACCTGACGCTGGGCACCTATTATATGAAACACGTGCTGTCCCTG
TTCGACAACCAGTCGCTGGCTTCGGCCGCATACAACGCCGGTCCGAGCCGCGTGTGGCAGTGGCGTGATGAGAAGCCGCT
GGAGGGCGCGATTTATGCGGAAACGATCCCCTTCAATGAAACGCGCGATTATGTCAAAAAGGTGATGAGCAACTCCATGT
ATTACGCCCGCAACTTCGGGCATCAGGTCAAGGCGCTGAAGCAGCGCCTGGGTATTGTCTCGTCCCGATTCGGGAGTGAA
AAAGTGTCGGAGTGA

Upstream 100 bases:

>100_bases
GGAAGCTGGTAAAAGGAAGTAAAGAAAAACGGCTTTCAAGTGAACCTGGAGCAGATACGCTCTTCAATTTTTTCCCTCGA
TTACTAATGTATAGCAGGTT

Downstream 100 bases:

>100_bases
GGGGTCTGACATGACATTCAAGAATATCTGCATTTTCGGCGGCAGTGGCTTTGTCGGGAAACATCTCGCAAATCTCCTGA
CCAACCGGGAAATCTACCTG

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MKKLLIGLFLATTSTATYAAARNQDFLALKHAYQAGNTARVNMYAQRLRGHVLEPYGAYYQLRPQLWNPKLSPDTVKQFI
EKYHDSPLSDRLLGEWLKVLGWTERWDLFAENYPRMVNKDVELVCYSLQQRLAGGDPTVYKEAFPLWFNAREMPHSCNQI
FDVLITAGTLTDDDIWARLRLALESGNVSAAKRINEYLPKNQRLNERNLNAATENPLRYLERHRREIKTRSDREIALFAM
VRLLRSGLDQAHAYWPRIRDRFGAPDQAYFLVQLADQGARRLDPRALQWFGEAANTHKSVPLTDAQLGWKTRTALRAGDW
NLVLDTIETMSAAEQKEGVWRYWKARALKAKGKLKEANAILAPLSSEHHFYGQLAEEELGVTIGAPAESFKPTAKEISAM
ERTPGIARALAFYNLDLRSEAAQEWIWAVRKFDDHELLAAAEVARRHGHYDRAINTADRTVQRHDFHLRFLAPHRDAMRD
ILKQQELDEAWVYGLIRQESRFVSDAKSSAGAMGLMQLMPGTAKWASKQMGLRKYRPSRVTEVNTNLTLGTYYMKHVLSL
FDNQSLASAAYNAGPSRVWQWRDEKPLEGAIYAETIPFNETRDYVKKVMSNSMYYARNFGHQVKALKQRLGIVSSRFGSE
KVSE

Sequences:

>Translated_644_residues
MKKLLIGLFLATTSTATYAAARNQDFLALKHAYQAGNTARVNMYAQRLRGHVLEPYGAYYQLRPQLWNPKLSPDTVKQFI
EKYHDSPLSDRLLGEWLKVLGWTERWDLFAENYPRMVNKDVELVCYSLQQRLAGGDPTVYKEAFPLWFNAREMPHSCNQI
FDVLITAGTLTDDDIWARLRLALESGNVSAAKRINEYLPKNQRLNERNLNAATENPLRYLERHRREIKTRSDREIALFAM
VRLLRSGLDQAHAYWPRIRDRFGAPDQAYFLVQLADQGARRLDPRALQWFGEAANTHKSVPLTDAQLGWKTRTALRAGDW
NLVLDTIETMSAAEQKEGVWRYWKARALKAKGKLKEANAILAPLSSEHHFYGQLAEEELGVTIGAPAESFKPTAKEISAM
ERTPGIARALAFYNLDLRSEAAQEWIWAVRKFDDHELLAAAEVARRHGHYDRAINTADRTVQRHDFHLRFLAPHRDAMRD
ILKQQELDEAWVYGLIRQESRFVSDAKSSAGAMGLMQLMPGTAKWASKQMGLRKYRPSRVTEVNTNLTLGTYYMKHVLSL
FDNQSLASAAYNAGPSRVWQWRDEKPLEGAIYAETIPFNETRDYVKKVMSNSMYYARNFGHQVKALKQRLGIVSSRFGSE
KVSE
>Mature_644_residues
MKKLLIGLFLATTSTATYAAARNQDFLALKHAYQAGNTARVNMYAQRLRGHVLEPYGAYYQLRPQLWNPKLSPDTVKQFI
EKYHDSPLSDRLLGEWLKVLGWTERWDLFAENYPRMVNKDVELVCYSLQQRLAGGDPTVYKEAFPLWFNAREMPHSCNQI
FDVLITAGTLTDDDIWARLRLALESGNVSAAKRINEYLPKNQRLNERNLNAATENPLRYLERHRREIKTRSDREIALFAM
VRLLRSGLDQAHAYWPRIRDRFGAPDQAYFLVQLADQGARRLDPRALQWFGEAANTHKSVPLTDAQLGWKTRTALRAGDW
NLVLDTIETMSAAEQKEGVWRYWKARALKAKGKLKEANAILAPLSSEHHFYGQLAEEELGVTIGAPAESFKPTAKEISAM
ERTPGIARALAFYNLDLRSEAAQEWIWAVRKFDDHELLAAAEVARRHGHYDRAINTADRTVQRHDFHLRFLAPHRDAMRD
ILKQQELDEAWVYGLIRQESRFVSDAKSSAGAMGLMQLMPGTAKWASKQMGLRKYRPSRVTEVNTNLTLGTYYMKHVLSL
FDNQSLASAAYNAGPSRVWQWRDEKPLEGAIYAETIPFNETRDYVKKVMSNSMYYARNFGHQVKALKQRLGIVSSRFGSE
KVSE

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=629, Percent_Identity=25.9141494435612, Blast_Score=165, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73874; Mature: 73874

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLIGLFLATTSTATYAAARNQDFLALKHAYQAGNTARVNMYAQRLRGHVLEPYGAYY
CCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCCE
QLRPQLWNPKLSPDTVKQFIEKYHDSPLSDRLLGEWLKVLGWTERWDLFAENYPRMVNKD
EECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHCCC
VELVCYSLQQRLAGGDPTVYKEAFPLWFNAREMPHSCNQIFDVLITAGTLTDDDIWARLR
HHHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
LALESGNVSAAKRINEYLPKNQRLNERNLNAATENPLRYLERHRREIKTRSDREIALFAM
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
VRLLRSGLDQAHAYWPRIRDRFGAPDQAYFLVQLADQGARRLDPRALQWFGEAANTHKSV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEHHCHHHHCCHHHHHHHHHHHCCCCCC
PLTDAQLGWKTRTALRAGDWNLVLDTIETMSAAEQKEGVWRYWKARALKAKGKLKEANAI
CCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEE
LAPLSSEHHFYGQLAEEELGVTIGAPAESFKPTAKEISAMERTPGIARALAFYNLDLRSE
ECCCCCCCHHHHHHHHHHCCEEECCCHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHH
AAQEWIWAVRKFDDHELLAAAEVARRHGHYDRAINTADRTVQRHDFHLRFLAPHRDAMRD
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHH
ILKQQELDEAWVYGLIRQESRFVSDAKSSAGAMGLMQLMPGTAKWASKQMGLRKYRPSRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCHHHCCCCCC
TEVNTNLTLGTYYMKHVLSLFDNQSLASAAYNAGPSRVWQWRDEKPLEGAIYAETIPFNE
EEECCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCCCCCCCCCCCEEEECCCCCH
TRDYVKKVMSNSMYYARNFGHQVKALKQRLGIVSSRFGSEKVSE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKLLIGLFLATTSTATYAAARNQDFLALKHAYQAGNTARVNMYAQRLRGHVLEPYGAYY
CCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCCE
QLRPQLWNPKLSPDTVKQFIEKYHDSPLSDRLLGEWLKVLGWTERWDLFAENYPRMVNKD
EECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHCCC
VELVCYSLQQRLAGGDPTVYKEAFPLWFNAREMPHSCNQIFDVLITAGTLTDDDIWARLR
HHHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
LALESGNVSAAKRINEYLPKNQRLNERNLNAATENPLRYLERHRREIKTRSDREIALFAM
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
VRLLRSGLDQAHAYWPRIRDRFGAPDQAYFLVQLADQGARRLDPRALQWFGEAANTHKSV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEHHCHHHHCCHHHHHHHHHHHCCCCCC
PLTDAQLGWKTRTALRAGDWNLVLDTIETMSAAEQKEGVWRYWKARALKAKGKLKEANAI
CCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEE
LAPLSSEHHFYGQLAEEELGVTIGAPAESFKPTAKEISAMERTPGIARALAFYNLDLRSE
ECCCCCCCHHHHHHHHHHCCEEECCCHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHH
AAQEWIWAVRKFDDHELLAAAEVARRHGHYDRAINTADRTVQRHDFHLRFLAPHRDAMRD
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHH
ILKQQELDEAWVYGLIRQESRFVSDAKSSAGAMGLMQLMPGTAKWASKQMGLRKYRPSRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCHHHCCCCCC
TEVNTNLTLGTYYMKHVLSLFDNQSLASAAYNAGPSRVWQWRDEKPLEGAIYAETIPFNE
EEECCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCCCCCCCCCCCEEEECCCCCH
TRDYVKKVMSNSMYYARNFGHQVKALKQRLGIVSSRFGSEKVSE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]