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The map label for this gene is gdhA [H]

Identifier: 82703562

GI number: 82703562

Start: 2795836

End: 2797179

Strand: Direct

Name: gdhA [H]

Synonym: Nmul_A2447

Alternate gene names: 82703562

Gene position: 2795836-2797179 (Clockwise)

Preceding gene: 82703560

Following gene: 82703564

Centisome position: 87.8

GC content: 56.1

Gene sequence:

>1344_bases
ATGAAATATCGCAGTTTCGAGGAATTCTGCGCGTACGTGGCGGAGCATAATCCGGGACAGCCCGAATATCTCCAGGCAGT
GACGGAAGTCATGGAGAGCCTCTGGCCCTTTATCACGAAAAACCCTCGTTATGCGGAACACGGCATTCTCGACCGTCTCG
TGGAGCCGGAGCGAATAATCGTTTTTCGTGTGTCCTGGGTGGATGATCGAGGAGAGGTGAAAGTCAACCGGGGCTACCGC
ATACAGCACAGCTCTTCCATTGGCCCATATAAGGGTGGCACCCGCTTTCATCCCTCGGTAAACCTTTCCATCCTCAAATT
CCTGGCGTTCGAGCAAACGTTCAAGAACGCGCTTACGACACTTCCCATGGGGGGTGGCAAGGGTGGCGCCGATTTCAACC
CCAGGGACCGTAGTCCGGGGGAGATCATGCGCTTCTGTCAGGCTTATATGACTGAGCTGTTCCGTCATATCGGCTCGGAT
GTCGATGTGCCGGCAGGCGATATCGGCGTGGGGGGGCGGGAAGTAGGTTTCATGGCAGGGATGATGAAAAAACTTTCCAA
TCGTGCTGACTGCGTCTTCACCGGGAAGGGGTTGAGTTTCGGTGGATCCCTCATTCGTCCGGAAGCTACCGGCTACGGTA
CTGTCTACTTTGCCGAGGAGATGCTCAAGCAATCCGGACGCTCGCTGGAAGGCATGCGTGTTGCGGTTTCAGGTTCGGGA
AACGTGGCGCAATATGCGGTGGAGAAAGCGATGGCCTTGGGTGCCAAAGTGATAACGGTTTCCGATTCAGACGGCACTGT
AGTCGATGAAGAAGGTTTTACTCCCGAAAAGCTCGCTGAACTGATGGATGTGAAAAATAAGCAGTATGGCCGTATCAATG
CCTACGCAGAGCGTGTGAAAGCCAATTTTATTCCCGGTAAAAGACCGTGGCATGTTCCCGTAGAAGTCGCGCTGCCCTGC
GCCACGCAGAACGAGCTGAATGAAGAGGATGCTGCCCTGCTTATCAGGAACGGCGTGGTATGCGTGGCAGAAGGCGCCAA
CATGCCTTCCACCGCCGAGGCGGTAAAGCAATTCGAGCGCAGCGGGGTTCTTTTTGCGCCGGGCAAGGCCAGCAATGCCG
GCGGCGTAGCCACTTCCGGTCTGGAAATGAGCCAGAACGCCATGCGTCTTTCCTGGCAGCGGGAAGAAGTGGACGCGCGA
TTGCTCCAGATCATGCAGGCTATCCACCGCTCCTGCACCGAGCACGGGCGGCGGGAAGACGGCACTATCAGCTACGTCAA
CGGAGCGAATGTCGCAGGTTTCGTAAAGGTGGCGGATGCGATGATAGGCCAGGGTGTAATCTAG

Upstream 100 bases:

>100_bases
CAATTCCGAACATAAATTCAGTGCGATTCCGCAAGTTATTTGGCAGAATACCTGTCTTGGGAAAATGGTTTAGCACAAGA
GGACATAAGGGGAATAACCA

Downstream 100 bases:

>100_bases
AGCCTGATCCAGCAGTCTCCGGTCCCGGTTCCACCCCTTCCTGTTTCAAGGAGGGGGGAAGAGAAAACAGGATGAGCCCG
CGCTGCAACGGGTTTTTTGG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKYRSFEEFCAYVAEHNPGQPEYLQAVTEVMESLWPFITKNPRYAEHGILDRLVEPERIIVFRVSWVDDRGEVKVNRGYR
IQHSSSIGPYKGGTRFHPSVNLSILKFLAFEQTFKNALTTLPMGGGKGGADFNPRDRSPGEIMRFCQAYMTELFRHIGSD
VDVPAGDIGVGGREVGFMAGMMKKLSNRADCVFTGKGLSFGGSLIRPEATGYGTVYFAEEMLKQSGRSLEGMRVAVSGSG
NVAQYAVEKAMALGAKVITVSDSDGTVVDEEGFTPEKLAELMDVKNKQYGRINAYAERVKANFIPGKRPWHVPVEVALPC
ATQNELNEEDAALLIRNGVVCVAEGANMPSTAEAVKQFERSGVLFAPGKASNAGGVATSGLEMSQNAMRLSWQREEVDAR
LLQIMQAIHRSCTEHGRREDGTISYVNGANVAGFVKVADAMIGQGVI

Sequences:

>Translated_447_residues
MKYRSFEEFCAYVAEHNPGQPEYLQAVTEVMESLWPFITKNPRYAEHGILDRLVEPERIIVFRVSWVDDRGEVKVNRGYR
IQHSSSIGPYKGGTRFHPSVNLSILKFLAFEQTFKNALTTLPMGGGKGGADFNPRDRSPGEIMRFCQAYMTELFRHIGSD
VDVPAGDIGVGGREVGFMAGMMKKLSNRADCVFTGKGLSFGGSLIRPEATGYGTVYFAEEMLKQSGRSLEGMRVAVSGSG
NVAQYAVEKAMALGAKVITVSDSDGTVVDEEGFTPEKLAELMDVKNKQYGRINAYAERVKANFIPGKRPWHVPVEVALPC
ATQNELNEEDAALLIRNGVVCVAEGANMPSTAEAVKQFERSGVLFAPGKASNAGGVATSGLEMSQNAMRLSWQREEVDAR
LLQIMQAIHRSCTEHGRREDGTISYVNGANVAGFVKVADAMIGQGVI
>Mature_447_residues
MKYRSFEEFCAYVAEHNPGQPEYLQAVTEVMESLWPFITKNPRYAEHGILDRLVEPERIIVFRVSWVDDRGEVKVNRGYR
IQHSSSIGPYKGGTRFHPSVNLSILKFLAFEQTFKNALTTLPMGGGKGGADFNPRDRSPGEIMRFCQAYMTELFRHIGSD
VDVPAGDIGVGGREVGFMAGMMKKLSNRADCVFTGKGLSFGGSLIRPEATGYGTVYFAEEMLKQSGRSLEGMRVAVSGSG
NVAQYAVEKAMALGAKVITVSDSDGTVVDEEGFTPEKLAELMDVKNKQYGRINAYAERVKANFIPGKRPWHVPVEVALPC
ATQNELNEEDAALLIRNGVVCVAEGANMPSTAEAVKQFERSGVLFAPGKASNAGGVATSGLEMSQNAMRLSWQREEVDAR
LLQIMQAIHRSCTEHGRREDGTISYVNGANVAGFVKVADAMIGQGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=410, Percent_Identity=31.9512195121951, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4885281, Length=410, Percent_Identity=30.7317073170732, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1788059, Length=443, Percent_Identity=68.3972911963883, Blast_Score=640, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=391, Percent_Identity=28.6445012787724, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324951, Length=451, Percent_Identity=49.6674057649667, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=48.4581497797357, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45549226, Length=393, Percent_Identity=31.0432569974555, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI45553475, Length=379, Percent_Identity=31.6622691292876, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24649283, Length=366, Percent_Identity=29.2349726775956, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24649460, Length=328, Percent_Identity=30.4878048780488, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48813; Mature: 48813

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYRSFEEFCAYVAEHNPGQPEYLQAVTEVMESLWPFITKNPRYAEHGILDRLVEPERII
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEE
VFRVSWVDDRGEVKVNRGYRIQHSSSIGPYKGGTRFHPSVNLSILKFLAFEQTFKNALTT
EEEEEEECCCCCEEECCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHH
LPMGGGKGGADFNPRDRSPGEIMRFCQAYMTELFRHIGSDVDVPAGDIGVGGREVGFMAG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
MMKKLSNRADCVFTGKGLSFGGSLIRPEATGYGTVYFAEEMLKQSGRSLEGMRVAVSGSG
HHHHHCCCCCEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCC
NVAQYAVEKAMALGAKVITVSDSDGTVVDEEGFTPEKLAELMDVKNKQYGRINAYAERVK
CHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
ANFIPGKRPWHVPVEVALPCATQNELNEEDAALLIRNGVVCVAEGANMPSTAEAVKQFER
HHCCCCCCCCCCEEEEEECCCCCCCCCCHHHEEEEECCEEEEECCCCCCHHHHHHHHHHH
SGVLFAPGKASNAGGVATSGLEMSQNAMRLSWQREEVDARLLQIMQAIHRSCTEHGRRED
CCCEECCCCCCCCCCCEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GTISYVNGANVAGFVKVADAMIGQGVI
CCEEEECCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKYRSFEEFCAYVAEHNPGQPEYLQAVTEVMESLWPFITKNPRYAEHGILDRLVEPERII
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEE
VFRVSWVDDRGEVKVNRGYRIQHSSSIGPYKGGTRFHPSVNLSILKFLAFEQTFKNALTT
EEEEEEECCCCCEEECCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHH
LPMGGGKGGADFNPRDRSPGEIMRFCQAYMTELFRHIGSDVDVPAGDIGVGGREVGFMAG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
MMKKLSNRADCVFTGKGLSFGGSLIRPEATGYGTVYFAEEMLKQSGRSLEGMRVAVSGSG
HHHHHCCCCCEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCC
NVAQYAVEKAMALGAKVITVSDSDGTVVDEEGFTPEKLAELMDVKNKQYGRINAYAERVK
CHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
ANFIPGKRPWHVPVEVALPCATQNELNEEDAALLIRNGVVCVAEGANMPSTAEAVKQFER
HHCCCCCCCCCCEEEEEECCCCCCCCCCHHHEEEEECCEEEEECCCCCCHHHHHHHHHHH
SGVLFAPGKASNAGGVATSGLEMSQNAMRLSWQREEVDARLLQIMQAIHRSCTEHGRRED
CCCEECCCCCCCCCCCEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GTISYVNGANVAGFVKVADAMIGQGVI
CCEEEECCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]