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The map label for this gene is ksgA

Identifier: 82701652

GI number: 82701652

Start: 588509

End: 589288

Strand: Reverse

Name: ksgA

Synonym: Nmul_A0518

Alternate gene names: 82701652

Gene position: 589288-588509 (Counterclockwise)

Preceding gene: 82701653

Following gene: 82701651

Centisome position: 18.51

GC content: 53.33

Gene sequence:

>780_bases
ATGCAGCACACCCCCCGCAGGCGTTTCGGCCAGAATTTCCTGGTTGACTCCCAAACCGTAACCGACATTGTTCACGCCCT
CCATCCCCGCCGGGAGGATGTAATGGTAGAGATCGGCCCAGGACTGGGAGCATTGACGCAGCCCCTGCTCCAGTCACTTG
AACATCTGCATGTGGTGGAGATCGACCGGGATATCGTGAAGCGGCTACGTAATGAATTTTCTGCGAAAAGGCTGACAGTA
CATGAAGGGGATGCGCTCGAATTCGATTTCTCCAGCCTCGGTGAAAGTCTGCGGGTAGTGGGCAATCTGCCCTACAACAT
TTCCACTCCGCTCCTTTTCCACCTGAGCCGGTTCACGGACCACCTCCGCGACATGCACTTCATGCTACAGAAAGAAGTGG
TCGCCCGCATGGTAGCCAAACCCTCTACTTCGGATTACGGACGGCTTTCAGTCATGCTGCAATGCCGCTTCGAGATGGAG
CAACTCTTCATCGTTCCGCCTGAATGCTTCCACCCGCCGCCAAAGGTCCAATCGGCCGTGGTTCGCATGATTCCACTCAA
GAAACCGCTAATCGAGGCGAGCCAGGAAAAATTGTTTGCGGAAATCGTTTCCGCCGCCTTCTCGCAGCGCCGCAAAATAC
TGCGCAACACGTTACGCGATTATCTTACGGGGGAGGATTATCTCAAGCTGGGAATCGACTCTAATCTTCGAGCGGAAAAC
TTATCGATTACGCAGTATGTAGCGATTACAAACTACTTTAATAGAGTCAGATCCGGTTGA

Upstream 100 bases:

>100_bases
GGCGGCATCGCTGGTTGCGCATCAGCGATAACTCTCAACCCTGCATCCCGTTCACCTGAGCTTGTCAAAGGATGAACGAA
CCTGCAAACCCTCATAAACC

Downstream 100 bases:

>100_bases
AGTCGATTGAAAGTAGCAGAAGTGTTACGCTAAGTTCTGAACTGAGATCAGAGCAAAAAGCCAATCGAACCAAGCCCTGT
CGCTCTCCGCTTCTCATTTA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MQHTPRRRFGQNFLVDSQTVTDIVHALHPRREDVMVEIGPGLGALTQPLLQSLEHLHVVEIDRDIVKRLRNEFSAKRLTV
HEGDALEFDFSSLGESLRVVGNLPYNISTPLLFHLSRFTDHLRDMHFMLQKEVVARMVAKPSTSDYGRLSVMLQCRFEME
QLFIVPPECFHPPPKVQSAVVRMIPLKKPLIEASQEKLFAEIVSAAFSQRRKILRNTLRDYLTGEDYLKLGIDSNLRAEN
LSITQYVAITNYFNRVRSG

Sequences:

>Translated_259_residues
MQHTPRRRFGQNFLVDSQTVTDIVHALHPRREDVMVEIGPGLGALTQPLLQSLEHLHVVEIDRDIVKRLRNEFSAKRLTV
HEGDALEFDFSSLGESLRVVGNLPYNISTPLLFHLSRFTDHLRDMHFMLQKEVVARMVAKPSTSDYGRLSVMLQCRFEME
QLFIVPPECFHPPPKVQSAVVRMIPLKKPLIEASQEKLFAEIVSAAFSQRRKILRNTLRDYLTGEDYLKLGIDSNLRAEN
LSITQYVAITNYFNRVRSG
>Mature_259_residues
MQHTPRRRFGQNFLVDSQTVTDIVHALHPRREDVMVEIGPGLGALTQPLLQSLEHLHVVEIDRDIVKRLRNEFSAKRLTV
HEGDALEFDFSSLGESLRVVGNLPYNISTPLLFHLSRFTDHLRDMHFMLQKEVVARMVAKPSTSDYGRLSVMLQCRFEME
QLFIVPPECFHPPPKVQSAVVRMIPLKKPLIEASQEKLFAEIVSAAFSQRRKILRNTLRDYLTGEDYLKLGIDSNLRAEN
LSITQYVAITNYFNRVRSG

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=214, Percent_Identity=33.1775700934579, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI156415992, Length=281, Percent_Identity=28.8256227758007, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1786236, Length=256, Percent_Identity=48.4375, Blast_Score=249, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI25141369, Length=291, Percent_Identity=26.8041237113402, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=184, Percent_Identity=35.3260869565217, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=179, Percent_Identity=32.4022346368715, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21358017, Length=189, Percent_Identity=34.9206349206349, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21357273, Length=278, Percent_Identity=28.0575539568345, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_NITMU (Q2YBP5)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411218.1
- ProteinModelPortal:   Q2YBP5
- SMR:   Q2YBP5
- STRING:   Q2YBP5
- GeneID:   3784507
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0518
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q2YBP5
- ProtClustDB:   PRK00274
- BioCyc:   NMUL323848:NMUL_A0518-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 29780; Mature: 29780

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 12-12 BINDING 14-14 BINDING 39-39 BINDING 60-60 BINDING 84-84 BINDING 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHTPRRRFGQNFLVDSQTVTDIVHALHPRREDVMVEIGPGLGALTQPLLQSLEHLHVVE
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
IDRDIVKRLRNEFSAKRLTVHEGDALEFDFSSLGESLRVVGNLPYNISTPLLFHLSRFTD
HCHHHHHHHHHHHHHCEEEEECCCCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
HLRDMHFMLQKEVVARMVAKPSTSDYGRLSVMLQCRFEMEQLFIVPPECFHPPPKVQSAV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHEEHHCCCCEEEECHHHCCCCHHHHHHH
VRMIPLKKPLIEASQEKLFAEIVSAAFSQRRKILRNTLRDYLTGEDYLKLGIDSNLRAEN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
LSITQYVAITNYFNRVRSG
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQHTPRRRFGQNFLVDSQTVTDIVHALHPRREDVMVEIGPGLGALTQPLLQSLEHLHVVE
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
IDRDIVKRLRNEFSAKRLTVHEGDALEFDFSSLGESLRVVGNLPYNISTPLLFHLSRFTD
HCHHHHHHHHHHHHHCEEEEECCCCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
HLRDMHFMLQKEVVARMVAKPSTSDYGRLSVMLQCRFEMEQLFIVPPECFHPPPKVQSAV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHEEHHCCCCEEEECHHHCCCCHHHHHHH
VRMIPLKKPLIEASQEKLFAEIVSAAFSQRRKILRNTLRDYLTGEDYLKLGIDSNLRAEN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
LSITQYVAITNYFNRVRSG
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA