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The map label for this gene is yjbJ [H]

Identifier: 82701651

GI number: 82701651

Start: 587670

End: 588296

Strand: Reverse

Name: yjbJ [H]

Synonym: Nmul_A0517

Alternate gene names: 82701651

Gene position: 588296-587670 (Counterclockwise)

Preceding gene: 82701652

Following gene: 82701650

Centisome position: 18.48

GC content: 53.27

Gene sequence:

>627_bases
ATGAGCTTGAAATCATCGACACCGCTGACCATAGCGATATTGCTGAGCTATGGATTGACACAGCCCGCATCTGCAGAGAT
CATCTACTCGTTTACCGATGAACATGGGGTGGTGCATTTCAGCAACGTCCCTGCGGATGAACGGTATGTTCCATTCATCG
GGAGCAGCGCCAACTCCAGGAAATCACGGACCGCTTCAAATAACGGGCATCCGGTCGCATCACTCAAGGCGCAATATGGT
TCGGTAATCAAGGAAGTCGCCCGCGCCCACGCCCTTGAAGACGCACTGCTCCACGCCGTGGTTACTGTCGAATCGGCCTA
CAACCCCAGGGCGGTTTCAAAAAAGGGTGCGGCGGGCCTGATGCAACTCATGCCGGAAACTGCCCAAAGATATGGCGTAA
TCGATCGCTTCGATCCCGTGCAGAATCTCCATGGCGGCGCAAGATATCTCACCGAGCTGCTGAGAATGTTCAACGGAAAT
CTGAGCCTCACCCTGGCCGCCTACAATGCTGGCGAAAACAACGTCATCAAGTACGGAAACCGGATTCCACCGTTTCAGGA
GACCAGAAATTACGTGCCCAAGGTCCTTGAACTCTATCGCAGATATCAGTCAAGAAAGAGGCTGTAA

Upstream 100 bases:

>100_bases
ATACCTGTTTATAAAAAGTAAATATTTAACATGAGCATGAGTATAATCTAGCAGCCACTTCGGGGCATACAAAAAAGGCG
AAAGCCTGGACTCCGGCTTT

Downstream 100 bases:

>100_bases
GAAGGGGACTCCTGGGATTTTAACGGGCAGCGAAACCGAGTTCCGGGGGAAGAAGAGTGAGAGTTCGCAGGCCGGGAATG
TTGCGTCCAAGGATGTGGTC

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSLKSSTPLTIAILLSYGLTQPASAEIIYSFTDEHGVVHFSNVPADERYVPFIGSSANSRKSRTASNNGHPVASLKAQYG
SVIKEVARAHALEDALLHAVVTVESAYNPRAVSKKGAAGLMQLMPETAQRYGVIDRFDPVQNLHGGARYLTELLRMFNGN
LSLTLAAYNAGENNVIKYGNRIPPFQETRNYVPKVLELYRRYQSRKRL

Sequences:

>Translated_208_residues
MSLKSSTPLTIAILLSYGLTQPASAEIIYSFTDEHGVVHFSNVPADERYVPFIGSSANSRKSRTASNNGHPVASLKAQYG
SVIKEVARAHALEDALLHAVVTVESAYNPRAVSKKGAAGLMQLMPETAQRYGVIDRFDPVQNLHGGARYLTELLRMFNGN
LSLTLAAYNAGENNVIKYGNRIPPFQETRNYVPKVLELYRRYQSRKRL
>Mature_207_residues
SLKSSTPLTIAILLSYGLTQPASAEIIYSFTDEHGVVHFSNVPADERYVPFIGSSANSRKSRTASNNGHPVASLKAQYGS
VIKEVARAHALEDALLHAVVTVESAYNPRAVSKKGAAGLMQLMPETAQRYGVIDRFDPVQNLHGGARYLTELLRMFNGNL
SLTLAAYNAGENNVIKYGNRIPPFQETRNYVPKVLELYRRYQSRKRL

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=123, Percent_Identity=36.5853658536585, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI87082441, Length=145, Percent_Identity=35.8620689655172, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 23012; Mature: 22881

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKSSTPLTIAILLSYGLTQPASAEIIYSFTDEHGVVHFSNVPADERYVPFIGSSANSR
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCEECCCCCCC
KSRTASNNGHPVASLKAQYGSVIKEVARAHALEDALLHAVVTVESAYNPRAVSKKGAAGL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHH
MQLMPETAQRYGVIDRFDPVQNLHGGARYLTELLRMFNGNLSLTLAAYNAGENNVIKYGN
HHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEECCC
RIPPFQETRNYVPKVLELYRRYQSRKRL
CCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLKSSTPLTIAILLSYGLTQPASAEIIYSFTDEHGVVHFSNVPADERYVPFIGSSANSR
CCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCEECCCCCCC
KSRTASNNGHPVASLKAQYGSVIKEVARAHALEDALLHAVVTVESAYNPRAVSKKGAAGL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHH
MQLMPETAQRYGVIDRFDPVQNLHGGARYLTELLRMFNGNLSLTLAAYNAGENNVIKYGN
HHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEECCC
RIPPFQETRNYVPKVLELYRRYQSRKRL
CCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]