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The map label for this gene is bphF [H]

Identifier: 82701563

GI number: 82701563

Start: 475928

End: 476689

Strand: Reverse

Name: bphF [H]

Synonym: Nmul_A0429

Alternate gene names: 82701563

Gene position: 476689-475928 (Counterclockwise)

Preceding gene: 82701564

Following gene: 82701562

Centisome position: 14.97

GC content: 55.77

Gene sequence:

>762_bases
GTGCAATTAAAAGCAAAACTGGCGCGCAACGCCTTGACCATCGGCTCCTGGATCACTTTGGGACATCCTTCCATCGCCGA
TATCATGGGCGCGGCGGGATTTGACTGGCTGGTGCTGGATACGGAACACAGCGTTCTGGAACTGAGCGAAGTTCAGATGC
TGATCCAGGTGCTCGACAGCCGTGCATGTCCCGCCATCGTGCGATTGACGTCGAATCATCCTGATCAGATCAAGCGGGTC
ATGGATGCAGGAGCGACAGGCGTAATGGTACCCATGATCAAAACGGCGGCGGATGCCGAAGCGGCCGTAAACGCAGTATA
TTACCCTCCACGCGGACAACGTGGAGTCGGGCTTGCACGCGCCCAGGGGTATGGCGCGCGATTTCAGCAATATCGCCACT
GGCTGGAAGAAAATGCCGTCATCATTGCCATGATCGAGCATATCGATGCAGTCGATGCCATCGATTCGATTCTTTCCGTT
CCGGGAATCGATGCTTATATCATAGGTCCCTACGATCTTTCAGGATCGCTCGGCCGTCCCGGAGAACTCGCTGATCCGGA
AGTCCAGGCTGCGGTAGAACGGGTAAAAGATGCCGGGCGACGCGCGGGCAAGGCAGGCGGCATTCATGTAGTCGAACCCA
ATCCGGAACAATTGCGCCGCAATATCGAGGCGGGCTTCAGTTTTCTTGGCTATGGCCTGGATATCCGCATTCTCGATACC
GTCTGCCGCAGTCATCTGCAAAACATCAGGGAAGCTCTATGA

Upstream 100 bases:

>100_bases
AGCGTGTAGCCAGACTGATGGAGAAAGACCCGTTACTCGCATCATACTGATGCGGACTTCATAATTCTCTCAGACGCACT
TCATTTCTCAAGGTGCATAC

Downstream 100 bases:

>100_bases
ACAAACTTGCGATTTCCACCTCGTCATTCGATGTCAGCCATAATAGTGCTCTCCGGCCTTTACAGGAGAGGGGCATACCC
ATTGCCGAAAACAGTCATAA

Product: 2-dehydro-3-deoxyglucarate aldolase

Products: NA

Alternate protein names: HOA [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MQLKAKLARNALTIGSWITLGHPSIADIMGAAGFDWLVLDTEHSVLELSEVQMLIQVLDSRACPAIVRLTSNHPDQIKRV
MDAGATGVMVPMIKTAADAEAAVNAVYYPPRGQRGVGLARAQGYGARFQQYRHWLEENAVIIAMIEHIDAVDAIDSILSV
PGIDAYIIGPYDLSGSLGRPGELADPEVQAAVERVKDAGRRAGKAGGIHVVEPNPEQLRRNIEAGFSFLGYGLDIRILDT
VCRSHLQNIREAL

Sequences:

>Translated_253_residues
MQLKAKLARNALTIGSWITLGHPSIADIMGAAGFDWLVLDTEHSVLELSEVQMLIQVLDSRACPAIVRLTSNHPDQIKRV
MDAGATGVMVPMIKTAADAEAAVNAVYYPPRGQRGVGLARAQGYGARFQQYRHWLEENAVIIAMIEHIDAVDAIDSILSV
PGIDAYIIGPYDLSGSLGRPGELADPEVQAAVERVKDAGRRAGKAGGIHVVEPNPEQLRRNIEAGFSFLGYGLDIRILDT
VCRSHLQNIREAL
>Mature_253_residues
MQLKAKLARNALTIGSWITLGHPSIADIMGAAGFDWLVLDTEHSVLELSEVQMLIQVLDSRACPAIVRLTSNHPDQIKRV
MDAGATGVMVPMIKTAADAEAAVNAVYYPPRGQRGVGLARAQGYGARFQQYRHWLEENAVIIAMIEHIDAVDAIDSILSV
PGIDAYIIGPYDLSGSLGRPGELADPEVQAAVERVKDAGRRAGKAGGIHVVEPNPEQLRRNIEAGFSFLGYGLDIRILDT
VCRSHLQNIREAL

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-oxovalerate to pyruvate and acetaldehyde (Probable) [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=253, Percent_Identity=33.9920948616601, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788578, Length=234, Percent_Identity=35.042735042735, Blast_Score=140, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 27410; Mature: 27410

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLKAKLARNALTIGSWITLGHPSIADIMGAAGFDWLVLDTEHSVLELSEVQMLIQVLDS
CCCHHHHHHHHHHHCCEEECCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCC
RACPAIVRLTSNHPDQIKRVMDAGATGVMVPMIKTAADAEAAVNAVYYPPRGQRGVGLAR
CCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHEEEECCCCCCCCCCHHH
AQGYGARFQQYRHWLEENAVIIAMIEHIDAVDAIDSILSVPGIDAYIIGPYDLSGSLGRP
HCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
GELADPEVQAAVERVKDAGRRAGKAGGIHVVEPNPEQLRRNIEAGFSFLGYGLDIRILDT
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHH
VCRSHLQNIREAL
HHHHHHHHHHHHC
>Mature Secondary Structure
MQLKAKLARNALTIGSWITLGHPSIADIMGAAGFDWLVLDTEHSVLELSEVQMLIQVLDS
CCCHHHHHHHHHHHCCEEECCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCC
RACPAIVRLTSNHPDQIKRVMDAGATGVMVPMIKTAADAEAAVNAVYYPPRGQRGVGLAR
CCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHEEEECCCCCCCCCCHHH
AQGYGARFQQYRHWLEENAVIIAMIEHIDAVDAIDSILSVPGIDAYIIGPYDLSGSLGRP
HCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
GELADPEVQAAVERVKDAGRRAGKAGGIHVVEPNPEQLRRNIEAGFSFLGYGLDIRILDT
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHH
VCRSHLQNIREAL
HHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9073078 [H]