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The map label for this gene is kdsB [H]

Identifier: 82701564

GI number: 82701564

Start: 476740

End: 477507

Strand: Reverse

Name: kdsB [H]

Synonym: Nmul_A0430

Alternate gene names: 82701564

Gene position: 477507-476740 (Counterclockwise)

Preceding gene: 82701567

Following gene: 82701563

Centisome position: 15.0

GC content: 54.17

Gene sequence:

>768_bases
ATGAAAACAATTGCTGTTATACCGGCCCGTATGGGTTCGTCCCGTTTTCCCGGAAAACCCATTGCGAACCTGCTGGGCCG
AACCATGCTCGAGCATGTGTATAAACGTGTCGCCTTGAGCAAAGCGCTTGATGCAACCTATATAGCCACCTGCGATGACG
AAATACGACGCGCCGCAGAGAATTTCGGTGCACCGGTCATTATGACTGCGGATACTCACGAACGTGCGAGTGACCGCGTG
GCCGAGGCGGTGGCAGAAATGGATGCTGAACTTATCGTCATGGTACAGGGCGATGAACCCATGACTCATCCGGAGATGAT
CGACGCAGCCGTGGATCCGTTCCGGCATGACCCGGACTTGGGGTGCGTGAATCTCATGCGGAGCATCGATAATAAAGGGG
ATTTCTACGACTTCAATACAATCAAGGTTGTGACAGACCGGCGTGGCGACGCTCTCTTCATGTCACGCCAACCGATTCCG
ACTGTGCCGGCGTCTGCTCTGTCCGCCGGATCTGATTTTCCGCATACCCTGGCCTTTAAACAGGTGTGCATCATTCCTTT
CCGCCGAAAAACGTTGCTCGAATTCTCCACGCTGCCGTCCACGCCGCTCGAAAAGCTTGAATCGGTGGACATGCTGCGGC
TATTGGAGCATGGTTATCGTGTAAAAATGGTGGAAACCCGGTTCAATACCCAAGCTGTCGACACCCAGGCCGACCTGGAG
CGTGTAGCCAGACTGATGGAGAAAGACCCGTTACTCGCATCATACTGA

Upstream 100 bases:

>100_bases
ATGCGCACCTTTAAAGGGCAACCTATCCCGATAAATAAAGGCAGTCATCAGTCAAAGTGAAAAGCTCGGCCTCATCGCCT
TCAATTAACATAAATCCCAC

Downstream 100 bases:

>100_bases
TGCGGACTTCATAATTCTCTCAGACGCACTTCATTTCTCAAGGTGCATACGTGCAATTAAAAGCAAAACTGGCGCGCAAC
GCCTTGACCATCGGCTCCTG

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKTIAVIPARMGSSRFPGKPIANLLGRTMLEHVYKRVALSKALDATYIATCDDEIRRAAENFGAPVIMTADTHERASDRV
AEAVAEMDAELIVMVQGDEPMTHPEMIDAAVDPFRHDPDLGCVNLMRSIDNKGDFYDFNTIKVVTDRRGDALFMSRQPIP
TVPASALSAGSDFPHTLAFKQVCIIPFRRKTLLEFSTLPSTPLEKLESVDMLRLLEHGYRVKMVETRFNTQAVDTQADLE
RVARLMEKDPLLASY

Sequences:

>Translated_255_residues
MKTIAVIPARMGSSRFPGKPIANLLGRTMLEHVYKRVALSKALDATYIATCDDEIRRAAENFGAPVIMTADTHERASDRV
AEAVAEMDAELIVMVQGDEPMTHPEMIDAAVDPFRHDPDLGCVNLMRSIDNKGDFYDFNTIKVVTDRRGDALFMSRQPIP
TVPASALSAGSDFPHTLAFKQVCIIPFRRKTLLEFSTLPSTPLEKLESVDMLRLLEHGYRVKMVETRFNTQAVDTQADLE
RVARLMEKDPLLASY
>Mature_255_residues
MKTIAVIPARMGSSRFPGKPIANLLGRTMLEHVYKRVALSKALDATYIATCDDEIRRAAENFGAPVIMTADTHERASDRV
AEAVAEMDAELIVMVQGDEPMTHPEMIDAAVDPFRHDPDLGCVNLMRSIDNKGDFYDFNTIKVVTDRRGDALFMSRQPIP
TVPASALSAGSDFPHTLAFKQVCIIPFRRKTLLEFSTLPSTPLEKLESVDMLRLLEHGYRVKMVETRFNTQAVDTQADLE
RVARLMEKDPLLASY

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria [H]

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family [H]

Homologues:

Organism=Escherichia coli, GI1787147, Length=250, Percent_Identity=32.8, Blast_Score=118, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329
- InterPro:   IPR004528 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: =2.7.7.38 [H]

Molecular weight: Translated: 28484; Mature: 28484

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIAVIPARMGSSRFPGKPIANLLGRTMLEHVYKRVALSKALDATYIATCDDEIRRAAE
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHH
NFGAPVIMTADTHERASDRVAEAVAEMDAELIVMVQGDEPMTHPEMIDAAVDPFRHDPDL
HCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCH
GCVNLMRSIDNKGDFYDFNTIKVVTDRRGDALFMSRQPIPTVPASALSAGSDFPHTLAFK
HHHHHHHHHCCCCCEEECCEEEEEECCCCCEEEECCCCCCCCCHHHHHCCCCCCHHHHHH
QVCIIPFRRKTLLEFSTLPSTPLEKLESVDMLRLLEHGYRVKMVETRFNTQAVDTQADLE
HHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHCCCHHHHH
RVARLMEKDPLLASY
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTIAVIPARMGSSRFPGKPIANLLGRTMLEHVYKRVALSKALDATYIATCDDEIRRAAE
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHH
NFGAPVIMTADTHERASDRVAEAVAEMDAELIVMVQGDEPMTHPEMIDAAVDPFRHDPDL
HCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCH
GCVNLMRSIDNKGDFYDFNTIKVVTDRRGDALFMSRQPIPTVPASALSAGSDFPHTLAFK
HHHHHHHHHCCCCCEEECCEEEEEECCCCCEEEECCCCCCCCCHHHHHCCCCCCHHHHHH
QVCIIPFRRKTLLEFSTLPSTPLEKLESVDMLRLLEHGYRVKMVETRFNTQAVDTQADLE
HHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHCCCHHHHH
RVARLMEKDPLLASY
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA